##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180931 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91662505599673 33.0 31.0 34.0 30.0 34.0 2 32.0767387872427 33.0 31.0 34.0 30.0 34.0 3 32.110783880828876 34.0 31.0 34.0 30.0 34.0 4 35.70138670136745 37.0 35.0 37.0 35.0 37.0 5 35.69725864779766 37.0 35.0 37.0 35.0 37.0 6 35.76675328105291 37.0 35.0 37.0 35.0 37.0 7 35.71435685035428 37.0 35.0 37.0 35.0 37.0 8 35.70255638532352 37.0 35.0 37.0 35.0 37.0 9 37.36866870157745 39.0 37.0 39.0 35.0 39.0 10-11 37.254306303133845 39.0 37.0 39.0 34.0 39.0 12-13 37.1436088532833 39.0 37.0 39.0 34.0 39.0 14-15 38.333126770172925 40.0 38.0 41.0 34.0 41.0 16-17 38.23775007095594 40.0 38.0 41.0 34.0 41.0 18-19 38.16401573456473 40.0 38.0 41.0 33.5 41.0 20-21 38.0619652799653 40.0 38.0 41.0 33.5 41.0 22-23 37.977328489530386 40.0 38.0 41.0 33.0 41.0 24-25 38.01953821556023 40.0 38.0 41.0 33.0 41.0 26-27 37.930271292397606 40.0 38.0 41.0 33.0 41.0 28-29 37.80739716203768 40.0 38.0 41.0 33.0 41.0 30-31 37.56690330872458 40.0 37.0 41.0 33.0 41.0 32-33 37.41741233445717 40.0 37.0 41.0 32.5 41.0 34-35 37.41412474764218 40.0 37.0 41.0 33.0 41.0 36-37 37.270643821377206 40.0 37.0 41.0 32.0 41.0 38-39 37.11763347854655 40.0 37.0 41.0 31.5 41.0 40-41 36.89196150634752 39.5 36.0 41.0 31.0 41.0 42-43 36.62048570083144 39.0 36.0 41.0 30.5 41.0 44-45 36.383238855332884 39.0 36.0 41.0 30.0 41.0 46-47 36.028072873465504 39.0 35.0 40.5 29.5 41.0 48-49 35.7043418613427 39.0 35.0 40.0 28.0 41.0 50-51 34.82280480216935 38.0 34.0 39.5 26.5 40.5 52-53 34.92452099584993 38.0 34.5 39.5 27.0 40.5 54-55 35.20637768916119 39.0 35.0 40.0 27.0 41.0 56-57 34.9654023900802 38.0 34.5 40.0 26.0 41.0 58-59 34.70193325694394 38.0 34.0 40.0 26.0 41.0 60-61 34.37230338786509 38.0 34.0 40.0 25.0 41.0 62-63 33.985651082037904 37.5 34.0 40.0 23.5 41.0 64-65 33.5822726624547 37.0 33.0 39.5 22.5 41.0 66-67 33.13359203019261 36.0 32.5 39.0 21.5 41.0 68-69 32.60609368201011 36.0 32.0 39.0 19.5 40.0 70-71 32.13518722050354 35.0 31.5 38.5 18.0 40.0 72-73 31.659215949518806 35.0 31.0 37.5 16.0 40.0 74-75 31.112308459093846 35.0 31.0 37.0 10.0 39.0 76-77 30.605968964630243 34.5 30.0 36.5 7.5 39.0 78-79 30.042458473009923 34.0 29.5 36.0 4.5 38.5 80-81 29.472183897610698 34.0 29.0 35.5 2.0 37.0 82-83 28.952943719906774 34.0 29.0 35.0 2.0 37.0 84-85 28.429299688986035 34.0 27.5 35.0 2.0 36.0 86-87 27.88065234525989 33.0 26.5 35.0 2.0 36.0 88-89 27.378547968734452 33.0 26.0 35.0 2.0 35.5 90-91 26.7974585165693 32.5 25.0 35.0 2.0 35.0 92-93 26.135498326173547 32.0 24.5 34.5 2.0 35.0 94-95 25.371137372067253 32.0 21.5 34.0 2.0 35.0 96-97 24.511647548684483 31.0 14.0 34.0 2.0 35.0 98-99 23.42176964791642 31.0 2.0 34.0 2.0 35.0 100-101 20.514256526226646 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12273760858607474 1101 2 0.19644200883306695 1101 3 0.18172107913297708 1101 4 0.07968909614812958 1101 5 -0.005118641634439314 1101 6 0.07665504234444143 1101 7 0.11114033153551617 1101 8 0.14000125538574082 1101 9 0.14890571425943477 1101 10-11 0.15516596451902132 1101 12-13 0.1425434992102126 1101 14-15 0.31912918057511774 1101 16-17 0.35001831734911093 1101 18-19 0.29796715763919934 1101 20-21 0.22802182007996663 1101 22-23 0.2639351734181332 1101 24-25 0.16572732529114376 1101 26-27 0.09295309098670401 1101 28-29 0.17515671639468877 1101 30-31 0.3230637703637669 1101 32-33 0.1618956335496975 1101 34-35 0.24011318847447427 1101 36-37 0.2810103778299933 1101 38-39 0.29811637898770726 1101 40-41 0.1790020353898285 1101 42-43 0.185492741219214 1101 44-45 0.3002137466332826 1101 46-47 0.24673077670580312 1101 48-49 0.27394012611277674 1101 50-51 0.24089690291402377 1101 52-53 0.1627475526883515 1101 54-55 0.32319526499419027 1101 56-57 0.11156629901387305 1101 58-59 0.16201736169606562 1101 60-61 0.204301274826868 1101 62-63 0.27635827667680246 1101 64-65 0.36182586603784017 1101 66-67 0.29382028267450977 1101 68-69 0.32043880827110627 1101 70-71 0.4653043699902568 1101 72-73 0.4317607339570273 1101 74-75 0.367837203483969 1101 76-77 0.32431448513852956 1101 78-79 0.4159970820713781 1101 80-81 0.6625424250219467 1101 82-83 0.6085871415301085 1101 84-85 0.5272370541760942 1101 86-87 0.619426343593446 1101 88-89 0.4415856400317679 1101 90-91 0.4892740622254017 1101 92-93 0.5426403621967317 1101 94-95 0.5267631417985363 1101 96-97 0.6562062250985008 1101 98-99 0.8329287462857948 1101 100-101 1.103695337727146 1102 1 0.21598421051061578 1102 2 0.3127443164526724 1102 3 0.3411184708405486 1102 4 0.19823758923973855 1102 5 0.07169617580576215 1102 6 0.11433181315377539 1102 7 0.11422717517974235 1102 8 0.14595287157599302 1102 9 0.180761748686173 1102 10-11 0.18330383098248149 1102 12-13 0.30671098918286077 1102 14-15 0.36382555788111404 1102 16-17 0.3857283836861569 1102 18-19 0.3425083805328626 1102 20-21 0.4037679272825301 1102 22-23 0.48198480953903555 1102 24-25 0.4083998097406294 1102 26-27 0.3297562898442621 1102 28-29 0.4430846656760892 1102 30-31 0.6029698064149756 1102 32-33 0.48966759034495766 1102 34-35 0.6030541555894899 1102 36-37 0.6957059096782743 1102 38-39 0.6091563030515772 1102 40-41 0.6709397284790839 1102 42-43 0.5890256468764719 1102 44-45 0.5644441106009808 1102 46-47 0.4986968350859513 1102 48-49 0.5579063897656269 1102 50-51 0.45637666140108735 1102 52-53 0.48400505474107547 1102 54-55 0.3768875523523363 1102 56-57 0.45744480341763705 1102 58-59 0.5014004249259685 1102 60-61 0.5196142677028703 1102 62-63 0.6554327183055193 1102 64-65 0.573081843313112 1102 66-67 0.5919025519863936 1102 68-69 0.49940845375481047 1102 70-71 0.4669147084982299 1102 72-73 0.5094906024643535 1102 74-75 0.5344423990985199 1102 76-77 0.5335094284891717 1102 78-79 0.6189644355893655 1102 80-81 0.779965333307878 1102 82-83 0.7909557854602411 1102 84-85 0.9191477775334711 1102 86-87 0.909193683968077 1102 88-89 0.8866089215005175 1102 90-91 0.9553401954783993 1102 92-93 0.8271641110669954 1102 94-95 1.0005436151957845 1102 96-97 1.131887501569821 1102 98-99 1.0790205349346564 1102 100-101 1.5576005401348922 1103 1 0.3263713709471183 1103 2 0.3204193222571803 1103 3 0.27485279867895684 1103 4 0.25065371415267634 1103 5 0.2203553353135419 1103 6 0.27634423479296544 1103 7 0.23052332098888684 1103 8 0.20144248151331112 1103 9 0.28038735815912474 1103 10-11 0.3397729510196328 1103 12-13 0.4222619817530884 1103 14-15 0.4536466340362111 1103 16-17 0.45696416265063533 1103 18-19 0.4115384478651123 1103 20-21 0.36562798068396773 1103 22-23 0.40767699667625834 1103 24-25 0.4291545404116306 1103 26-27 0.41812914217766917 1103 28-29 0.4756319723860969 1103 30-31 0.5784421479520105 1103 32-33 0.42884944546453596 1103 34-35 0.5864126417747642 1103 36-37 0.5277670891535493 1103 38-39 0.538598925526621 1103 40-41 0.7095933737077758 1103 42-43 0.5496943257712914 1103 44-45 0.45659279297850475 1103 46-47 0.6959157519654511 1103 48-49 0.6924758406595188 1103 50-51 0.5661782924667946 1103 52-53 0.6840152126235992 1103 54-55 0.7361297743165878 1103 56-57 0.7233150437241207 1103 58-59 0.6651766903079022 1103 60-61 0.8347952231242743 1103 62-63 0.8977997951571552 1103 64-65 0.9888635649290904 1103 66-67 1.0237337279789642 1103 68-69 0.9245513896730841 1103 70-71 0.8385778440914535 1103 72-73 0.7365004120766088 1103 74-75 0.649436884819437 1103 76-77 0.7940017955660217 1103 78-79 0.8864831551157195 1103 80-81 0.8925473219310476 1103 82-83 0.9641962950957179 1103 84-85 1.0228038896764922 1103 86-87 0.8784806006153971 1103 88-89 1.0102434118650372 1103 90-91 1.0297887675703414 1103 92-93 1.0933768171268952 1103 94-95 1.0682212330272236 1103 96-97 1.2512672902858668 1103 98-99 1.152574487802081 1103 100-101 1.3711859824381172 1104 1 0.2543428272605688 1104 2 0.29923502342662545 1104 3 0.26890024773868504 1104 4 0.27459742640261453 1104 5 0.24702883209362625 1104 6 0.14520926984786797 1104 7 0.238094037651031 1104 8 0.23410065694488935 1104 9 0.3246543465788747 1104 10-11 0.23818923334368236 1104 12-13 0.3303808328661475 1104 14-15 0.43022464011905726 1104 16-17 0.3140038796411986 1104 18-19 0.24779510544082228 1104 20-21 0.3624278821589897 1104 22-23 0.34175164719815854 1104 24-25 0.29484821339436706 1104 26-27 0.24798988639825126 1104 28-29 0.20773719421008963 1104 30-31 0.2854213577531013 1104 32-33 0.347376604089753 1104 34-35 0.2420001862180996 1104 36-37 0.3128648287905733 1104 38-39 0.31865529181109054 1104 40-41 0.3183958311207107 1104 42-43 0.3316324834969393 1104 44-45 0.27965567047911577 1104 46-47 0.36398384129476113 1104 48-49 0.2747202825654753 1104 50-51 0.29188653001195064 1104 52-53 0.3755718716272085 1104 54-55 0.462148758224167 1104 56-57 0.49816797020162795 1104 58-59 0.5285602023664993 1104 60-61 0.6249468302237844 1104 62-63 0.7145361908563217 1104 64-65 0.7061607676763586 1104 66-67 0.6891473544122135 1104 68-69 0.7262200672964951 1104 70-71 0.6905483437130542 1104 72-73 0.8236095249234126 1104 74-75 0.8486572523604501 1104 76-77 0.8222793575725049 1104 78-79 0.7180250710696257 1104 80-81 0.8966346807476064 1104 82-83 0.9397413952627787 1104 84-85 0.8893261006341859 1104 86-87 1.0328712093284338 1104 88-89 1.0630260286314197 1104 90-91 1.0829649487991908 1104 92-93 1.0243691707316245 1104 94-95 1.2241172034982881 1104 96-97 1.1348357508687315 1104 98-99 1.143842889841121 1104 100-101 1.1296193281465676 1105 1 0.09225121641499712 1105 2 0.1712449943411869 1105 3 0.07242334752940138 1105 4 0.1587238055067175 1105 5 0.11561806896349225 1105 6 0.07136732746867125 1105 7 0.12992058762941383 1105 8 0.10339519236105588 1105 9 0.13923374103278263 1105 10-11 0.15560577602362713 1105 12-13 0.1513375122310734 1105 14-15 0.2624267525495654 1105 16-17 0.19429677706104798 1105 18-19 0.12119979159741234 1105 20-21 0.15157249290566455 1105 22-23 0.24113813852918753 1105 24-25 0.14524352709339894 1105 26-27 0.1024440957744659 1105 28-29 0.1394995598550537 1105 30-31 0.24340772679389033 1105 32-33 0.2851913986110972 1105 34-35 0.32583957306317046 1105 36-37 0.24834571317864373 1105 38-39 0.30040578216321734 1105 40-41 0.20008416568488485 1105 42-43 0.133078128946174 1105 44-45 0.14940301911244092 1105 46-47 0.2683898997876213 1105 48-49 0.33443152886481897 1105 50-51 0.3708785233935501 1105 52-53 0.30971471010373364 1105 54-55 0.3576319238235115 1105 56-57 0.43484576860175395 1105 58-59 0.5447983117332527 1105 60-61 0.7079951926245158 1105 62-63 0.8253846498475781 1105 64-65 0.6950561055591251 1105 66-67 0.7342861004246046 1105 68-69 0.5707688747499873 1105 70-71 0.5691131875042288 1105 72-73 0.5982422751762613 1105 74-75 0.6831717983308288 1105 76-77 0.7432794232656121 1105 78-79 0.7663921641639817 1105 80-81 0.7160481134724463 1105 82-83 0.8641367479445385 1105 84-85 0.7441217697979141 1105 86-87 0.8423193222397245 1105 88-89 0.9724494885791977 1105 90-91 0.8205144803206927 1105 92-93 1.0241341915332391 1105 94-95 1.3724652383719445 1105 96-97 1.2465427321692957 1105 98-99 1.141249636306867 1105 100-101 1.1597298730456451 1106 1 0.07662553150753482 1106 2 0.10044326130468306 1106 3 -0.008829709703547906 1106 4 0.14196852030009666 1106 5 0.12154672492921037 1106 6 0.11309892093824203 1106 7 0.08951235659822032 1106 8 0.11384283139169327 1106 9 0.1989489436606604 1106 10-11 0.07676194442871775 1106 12-13 0.08692934792077978 1106 14-15 0.11021960976723477 1106 16-17 0.1139370816031473 1106 18-19 0.17832791084781974 1106 20-21 0.15755555355611506 1106 22-23 0.1242916163753307 1106 24-25 0.07674318010595016 1106 26-27 0.14948785576078194 1106 28-29 0.22101814298250133 1106 30-31 0.260241758175205 1106 32-33 0.3397301070109009 1106 34-35 0.41147603805040234 1106 36-37 0.44500090152359206 1106 38-39 0.4456859769572077 1106 40-41 0.42171740789764556 1106 42-43 0.4872711493972943 1106 44-45 0.5553562299198305 1106 46-47 0.5603489067789624 1106 48-49 0.6068037825919816 1106 50-51 0.5835411992196029 1106 52-53 0.5699499672099932 1106 54-55 0.5555396806320445 1106 56-57 0.5719172565514512 1106 58-59 0.46358123335598833 1106 60-61 0.41130666322678167 1106 62-63 0.5031718862176078 1106 64-65 0.7658991512439357 1106 66-67 0.8042263774637419 1106 68-69 0.7214576322400887 1106 70-71 0.5789492829663132 1106 72-73 0.827868448077254 1106 74-75 0.785250697342935 1106 76-77 0.8721473442075585 1106 78-79 0.8650559812276981 1106 80-81 0.8956931583015191 1106 82-83 0.7974416473142973 1106 84-85 0.6280977184662682 1106 86-87 0.6961414986365888 1106 88-89 0.87250507639612 1106 90-91 0.9522236641030162 1106 92-93 0.9886573912036702 1106 94-95 0.9749129448993017 1106 96-97 1.2582736817046367 1106 98-99 1.2035849918264496 1106 100-101 1.1124834289397185 1107 1 0.1238219172852375 1107 2 0.11395717834590613 1107 3 0.0881866537540077 1107 4 0.037736134437992064 1107 5 0.10728309644527201 1107 6 0.0564371793541838 1107 7 0.08233674614876207 1107 8 0.08561060160334222 1107 9 0.09469426154746685 1107 10-11 0.05376774105585724 1107 12-13 0.1260194538399091 1107 14-15 0.19821027161950866 1107 16-17 0.1927457690188632 1107 18-19 0.26464486081622596 1107 20-21 0.1956111731470358 1107 22-23 0.17647907768039062 1107 24-25 0.10566908209879955 1107 26-27 0.07428289946342659 1107 28-29 -0.09763509974842322 1107 30-31 0.20202226512029142 1107 32-33 0.24311069645211347 1107 34-35 0.2628677421727801 1107 36-37 0.2936392177107052 1107 38-39 0.1402314181104387 1107 40-41 0.28317218652175313 1107 42-43 0.2690490803290757 1107 44-45 0.3732026270646429 1107 46-47 0.43333711803435193 1107 48-49 0.3957251735754568 1107 50-51 0.3186755719563834 1107 52-53 0.24913100201882088 1107 54-55 0.269050372679807 1107 56-57 0.32612089617555284 1107 58-59 0.30263644166070236 1107 60-61 0.36333170032416007 1107 62-63 0.40859984882202127 1107 64-65 0.4391002120677072 1107 66-67 0.5496946644308238 1107 68-69 0.5403987354984068 1107 70-71 0.4468921711191598 1107 72-73 0.5792085124895294 1107 74-75 0.5444102759915666 1107 76-77 0.44740485595389856 1107 78-79 0.5710250658829779 1107 80-81 0.6712729305055021 1107 82-83 0.6099686964492648 1107 84-85 0.6729596668480582 1107 86-87 0.8581261516491914 1107 88-89 0.9615466481544139 1107 90-91 1.139517946348871 1107 92-93 1.3390696528676962 1107 94-95 1.4460216817251066 1107 96-97 1.7608516857362773 1107 98-99 1.5623206231866043 1107 100-101 1.1686232988968328 1108 1 -0.04976202955069198 1108 2 0.015442731051777514 1108 3 0.051246955369720126 1108 4 -0.001676794321902264 1108 5 0.05304616143656915 1108 6 0.0812467475344647 1108 7 0.10858127997904887 1108 8 0.08086941051650598 1108 9 0.1369536732000185 1108 10-11 0.17454760433242456 1108 12-13 0.24381733318132603 1108 14-15 0.2727173937849372 1108 16-17 0.23636960889930236 1108 18-19 0.2619481017705283 1108 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2316 64-65 -0.3328500312737859 2316 66-67 -0.44434369773821203 2316 68-69 -0.3676068211249799 2316 70-71 -0.5120499319937295 2316 72-73 -0.3428032214881611 2316 74-75 -0.44454512374333177 2316 76-77 -0.5776771246270407 2316 78-79 -0.39229793302949645 2316 80-81 -0.4819976807147519 2316 82-83 -0.44377237222304444 2316 84-85 -0.65331576460569 2316 86-87 -0.5673476549207379 2316 88-89 -0.518282717410333 2316 90-91 -0.49444094395732563 2316 92-93 -0.64264353949482 2316 94-95 -0.6980265821837222 2316 96-97 -0.5061497679056899 2316 98-99 -1.206011532754978 2316 100-101 -0.8562808248033562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8153.0 3 1477.0 4 1343.0 5 1576.0 6 1763.0 7 2002.0 8 2492.0 9 2976.0 10 3866.0 11 5037.0 12 6781.0 13 8866.0 14 10523.0 15 13482.0 16 16845.0 17 21945.0 18 19213.0 19 14109.0 20 13827.0 21 14471.0 22 15839.0 23 17812.0 24 20049.0 25 22580.0 26 26107.0 27 31575.0 28 37316.0 29 45622.0 30 56142.0 31 71157.0 32 91164.0 33 121061.0 34 166325.0 35 233390.0 36 329994.0 37 417711.0 38 280190.0 39 26148.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33959578445823 19.029174437241732 14.587540558377619 23.043689219922413 2 15.210502094377798 19.722597810040785 40.931328039192515 24.1355720563889 3 19.521588513710768 24.068980260813795 29.375090512455664 27.034340713019773 4 12.530240572895067 16.229268368935447 37.1685720564693 34.07191900170019 5 13.606215086463951 37.3534200494106 34.11469457251279 14.925670291612658 6 32.13823560891736 35.92767031968546 17.3358151260427 14.59827894535447 7 28.60505412243743 32.668509028368575 20.365140409643733 18.361296439550262 8 26.246936548952053 35.18507034165949 19.46009761699684 19.107895492391616 9 26.023047156359784 17.052148715988768 20.549737979391143 36.3750661482603 10-11 24.911474015073765 26.84468949751971 26.94603268524533 21.29780380216119 12-13 25.633670638721263 24.073994962697963 27.861043777122124 22.43129062145865 14-15 22.619377374030808 25.466974070496168 26.301746349579524 25.6119022058935 16-17 21.835030461950357 28.883979062979197 26.95289997216115 22.328090502909298 18-19 21.75234094020712 27.190874048241202 29.607596660750918 21.449188350800764 20-21 22.337086867941743 26.307385018672747 29.909051177401537 21.44647693598398 22-23 21.996717679060115 26.188153329309667 29.854651883820722 21.9604771078095 24-25 21.834890451302005 26.35165411104178 29.96347572891793 21.84997970873829 26-27 21.572128640858086 26.693777269259407 29.82131070661343 21.912783383269083 28-29 21.521408931621743 26.866779963190496 29.917793028428026 21.694018076759736 30-31 21.705489923685725 26.51629373186548 29.863771759123992 21.914444585324798 32-33 21.455277577745544 26.541341207520908 29.853162940324545 22.150218274409006 34-35 22.015551133579834 26.65668085215857 29.660625486245696 21.667142528015905 36-37 21.946485999388223 26.455681493545562 29.567181166070043 22.03065134099617 38-39 21.63455245627209 26.69711603597894 29.69178624818303 21.976545259565945 40-41 21.994947813206874 26.628744646404336 29.555223846321667 21.821083694067127 42-43 21.69458001818504 26.597948751633222 29.482314373322428 22.225156856859314 44-45 21.804199575375453 26.80304660304954 29.394225583858756 21.99852823771625 46-47 21.98791597130445 27.07227127463856 28.84153458804009 22.098278166016897 48-49 22.041941646604442 27.070106850003466 28.783974290954845 22.103977212437247 50-51 22.073572987636776 27.308426502923076 28.583542786426293 22.034457723013855 52-53 22.1821230210512 27.34785940871381 28.410114280043082 22.05990329019191 54-55 21.956978361949663 27.323177181775883 28.57116938437958 22.14867507189488 56-57 22.05841829705153 27.586640029392857 28.337673371911453 22.01726830164416 58-59 22.096843157219375 27.221379472829256 28.478798308255225 22.202979061696144 60-61 22.021488725216003 27.322419052185197 28.724729209548137 21.93136301305066 62-63 21.90296243667225 27.395340143099705 28.76040880643237 21.941288613795678 64-65 21.840686277886697 27.28240717140001 28.6636475068969 22.213259043816393 66-67 21.79045831044751 27.44216239352351 28.47962503375468 22.287754262274305 68-69 21.846849639845328 27.322495853731688 28.626337059578123 22.20431744684486 70-71 21.882802537824762 27.353721324722663 28.499389058346136 22.26408707910644 72-73 21.898218512834912 27.36624035553049 28.42611304772929 22.309428083905306 74-75 21.82591878518554 27.69038195340652 28.22891761038236 22.25478165102559 76-77 21.92735775225414 27.461503273737353 28.276148563744187 22.334990410264325 78-79 22.024546175014418 27.44332455235255 28.23175267258807 22.300376600044967 80-81 21.893562051603062 27.74811077107912 28.123717656233243 22.234609521084572 82-83 22.06346505486158 27.661084638826104 28.087366118246038 22.188084188066277 84-85 22.036680921902356 27.612433627048595 28.046519904709967 22.304365546339085 86-87 22.006864753698434 27.62306812564304 28.146867798271114 22.223199322387412 88-89 22.0741345908995 27.5768820726329 27.982339700382013 22.36664363608559 90-91 22.10551212925626 27.618674737528586 27.983930228072268 22.291882905142877 92-93 22.079329889864884 27.413759254943336 28.126279389360302 22.380631465831478 94-95 21.96391774119402 27.371916661040867 28.146049381185218 22.51811621657989 96-97 22.069681621886122 27.347433822298566 28.023280938897756 22.559603616917556 98-99 21.942626278584445 27.351373743460115 27.914979645523484 22.791020332431955 100-101 22.36234251103496 26.6991635332261 28.367872733258608 22.570621222480327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2678.0 1 1574.5 2 612.0 3 749.0 4 603.0 5 509.5 6 937.0 7 1945.5 8 3231.5 9 3816.0 10 3191.5 11 2575.0 12 2585.5 13 2764.5 14 3053.0 15 3238.5 16 3288.5 17 3441.0 18 3620.0 19 3866.0 20 4351.0 21 5083.0 22 5968.5 23 7128.0 24 8707.0 25 11079.5 26 13916.0 27 17079.5 28 19935.5 29 22966.0 30 27187.5 31 31116.0 32 35651.5 33 39557.5 34 43229.5 35 47792.5 36 52897.0 37 57766.0 38 60797.5 39 63144.0 40 65245.5 41 69199.0 42 72215.0 43 74391.0 44 77899.0 45 80693.0 46 82349.5 47 83723.5 48 85097.0 49 83510.5 50 79895.0 51 75803.0 52 72738.0 53 71069.0 54 69093.5 55 64032.5 56 56893.5 57 51106.5 58 46581.5 59 41588.0 60 34708.0 61 28275.0 62 22723.0 63 17161.0 64 12963.5 65 10113.0 66 7426.5 67 5533.0 68 4203.5 69 2983.5 70 2254.5 71 1702.0 72 1272.5 73 1087.5 74 871.0 75 648.0 76 499.5 77 345.0 78 210.5 79 155.0 80 110.5 81 65.5 82 51.0 83 43.0 84 23.5 85 13.0 86 14.5 87 15.0 88 9.5 89 6.5 90 5.5 91 5.5 92 4.5 93 3.0 94 3.0 95 3.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3024396461877978 2 0.2669502152979622 3 0.26667510343059914 4 0.26928866617054825 5 0.2794219532850879 6 0.1838664313543161 7 0.18409569124378533 8 0.18597562233743295 9 0.1835454675090592 10-11 0.19115689583943737 12-13 0.18212405619435001 14-15 0.1829493917964392 16-17 0.18877259298895746 18-19 0.18223868613908462 20-21 0.1754984453886895 22-23 0.17483359170922877 24-25 0.1781578601065325 26-27 0.17423751599660878 28-29 0.18558588052533528 30-31 0.18996474441419742 32-33 0.1925095291873058 34-35 0.1915007856736412 36-37 0.16823090689251516 38-39 0.1625911136115723 40-41 0.1676348311798952 42-43 0.17728667252654945 44-45 0.18106946070279162 46-47 0.18377472739852843 48-49 0.18129872059226082 50-51 0.18485224887903376 52-53 0.2073884960138583 54-55 0.1739624041292457 56-57 0.16190333394316464 58-59 0.16204088987684617 60-61 0.15667620846326638 62-63 0.1635998571252369 64-65 0.16066533054003082 66-67 0.15841858362323247 68-69 0.1683684628261967 70-71 0.16298085542367 72-73 0.15830395367849784 74-75 0.16254526163367847 76-77 0.16520467635152142 78-79 0.16907916848355128 80-81 0.1614677401531731 82-83 0.16032144070582702 84-85 0.15445238753541493 86-87 0.15064667337022583 88-89 0.15550698302697336 90-91 0.16282037350104153 92-93 0.15491090731435336 94-95 0.15873954746848937 96-97 0.15779958192166557 98-99 0.16153651812001388 100-101 0.1412240919130408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180931.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.394485615754995 #Duplication Level Percentage of deduplicated Percentage of total 1 78.086226603761 44.81718809598146 2 12.525811939683207 14.378250663956003 3 3.99799172998758 6.883880365160387 4 1.7584635413416168 4.037044417174441 5 0.9313200264549619 2.672631693101694 6 0.5791792599013248 1.9945017420810132 7 0.38091879634483045 1.5303846864308857 8 0.2644280695519598 1.2141370427441454 9 0.20148448031003696 1.0407688296257058 >10 1.1496018129069523 12.186030756242848 >50 0.0862046817189796 3.4067073168387614 >100 0.03597277591237755 3.7188099385887754 >500 0.0019170257001931853 0.7602386800056677 >1k 2.396282125241396E-4 0.29464234435242675 >5k 1.5975214168275975E-4 0.6008833370821935 >10k+ 7.987607084137987E-5 0.46390009063360926 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10119 0.4639761643078117 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7786 0.3570034998814726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.24397837437314615 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2739 0.12558856745123986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.585197789384442E-5 0.0 10-11 0.0 0.0 0.0 4.585197789384442E-5 0.0 12-13 0.0 0.0 0.0 4.585197789384442E-5 4.585197789384442E-5 14-15 4.585197789384442E-5 0.0 0.0 4.585197789384442E-5 4.585197789384442E-5 16-17 4.585197789384442E-5 0.0 0.0 4.585197789384442E-5 4.585197789384442E-5 18-19 4.585197789384442E-5 0.0 0.0 6.877796684076662E-5 4.585197789384442E-5 20-21 4.585197789384442E-5 0.0 0.0 9.170395578768883E-5 4.585197789384442E-5 22-23 4.585197789384442E-5 0.0 0.0 9.170395578768883E-5 9.170395578768883E-5 24-25 4.585197789384442E-5 0.0 0.0 1.3755593368153325E-4 9.170395578768883E-5 26-27 4.585197789384442E-5 0.0 0.0 1.8340791157537767E-4 9.170395578768883E-5 28-29 4.585197789384442E-5 0.0 0.0 5.272977457792108E-4 9.170395578768883E-5 30-31 4.585197789384442E-5 0.0 0.0 0.0012838553810276437 9.170395578768883E-5 32-33 4.585197789384442E-5 0.0 0.0 0.003095008507834498 9.170395578768883E-5 34-35 4.585197789384442E-5 0.0 0.0 0.005571015314102097 9.170395578768883E-5 36-37 4.585197789384442E-5 0.0 0.0 0.009720619313495016 9.170395578768883E-5 38-39 4.585197789384442E-5 0.0 0.0 0.01659841599757168 9.170395578768883E-5 40-41 4.585197789384442E-5 0.0 0.0 0.025081031907932896 9.170395578768883E-5 42-43 4.585197789384442E-5 0.0 0.0 0.03399924160828564 9.170395578768883E-5 44-45 4.585197789384442E-5 0.0 0.0 0.04479738240228599 9.170395578768883E-5 46-47 4.585197789384442E-5 0.0 0.0 0.0579110480799255 9.170395578768883E-5 48-49 4.585197789384442E-5 0.0 0.0 0.07207930924912342 1.8340791157537767E-4 50-51 4.585197789384442E-5 0.0 0.0 0.09016791452824505 1.8340791157537767E-4 52-53 4.585197789384442E-5 0.0 0.0 0.10979256106681046 1.8340791157537767E-4 54-55 4.585197789384442E-5 0.0 0.0 0.13319999578161804 1.8340791157537767E-4 56-57 4.585197789384442E-5 0.0 0.0 0.1634852271805023 1.8340791157537767E-4 58-59 4.585197789384442E-5 0.0 0.0 0.1960172054961849 1.8340791157537767E-4 60-61 4.585197789384442E-5 0.0 0.0 0.23577087033014799 2.292598894692221E-4 62-63 4.585197789384442E-5 0.0 0.0 0.2756391651088457 2.292598894692221E-4 64-65 4.585197789384442E-5 0.0 0.0 0.32039069553323785 2.292598894692221E-4 66-67 4.585197789384442E-5 0.0 0.0 0.37477114131533734 2.292598894692221E-4 68-69 4.585197789384442E-5 0.0 0.0 0.4404540996482695 2.751118673630665E-4 70-71 9.170395578768883E-5 2.292598894692221E-5 0.0 0.5123270749968706 2.751118673630665E-4 72-73 9.170395578768883E-5 6.877796684076662E-5 0.0 0.5902754374164061 3.209638452569109E-4 74-75 9.170395578768883E-5 9.170395578768883E-5 0.0 0.6746430767410798 3.6681582315075534E-4 76-77 9.170395578768883E-5 9.170395578768883E-5 0.0 0.7711385642186754 3.8974181209767755E-4 78-79 9.170395578768883E-5 9.170395578768883E-5 0.0 0.8843012456606834 4.1266780104459976E-4 80-81 9.170395578768883E-5 9.170395578768883E-5 0.0 1.0148189007355115 4.35593789991522E-4 82-83 9.170395578768883E-5 9.170395578768883E-5 0.0 1.1506553852460257 5.50223734726133E-4 84-85 9.170395578768883E-5 9.170395578768883E-5 0.0 1.2875235392591513 5.50223734726133E-4 86-87 9.170395578768883E-5 9.170395578768883E-5 0.0 1.4360610216462604 5.960757126199774E-4 88-89 9.170395578768883E-5 9.170395578768883E-5 0.0 1.6122243207144105 6.419276905138218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5425 0.0 43.9226 1 GTATCAA 8970 0.0 38.388065 1 ATCAACG 11450 0.0 29.64306 3 TCAACGC 11640 0.0 29.242218 4 TATCAAC 11745 0.0 29.222977 2 CAACGCA 11845 0.0 28.736788 5 AACGCAG 12120 0.0 28.142494 6 GTACATG 8820 0.0 24.48146 1 TACATGG 8675 0.0 24.440248 2 CGCAGAG 14060 0.0 24.055008 8 ACGCAGA 14145 0.0 23.979826 7 ACATGGG 8870 0.0 22.72336 3 GCAGAGT 14965 0.0 22.188158 9 GAGTACT 8710 0.0 21.733566 12-13 CATGGGG 4160 0.0 21.141151 4 GTACTTT 9065 0.0 20.855043 14-15 AGTACTT 9100 0.0 20.828228 12-13 TGGTATC 2970 0.0 20.487728 2 AGAGTAC 13605 0.0 20.320774 10-11 GAGTACA 7345 0.0 19.03731 1 >>END_MODULE