FastQCFastQC Report
Thu 26 May 2016
SRR937527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180931
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109190.5006577466228872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95250.43674008943886805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66760.30610780441930535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.11385046111041569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62850.044.7608071
GTATCAA104150.036.866641
ATCAACG132450.028.7696233
TATCAAC133400.028.6003572
TCAACGC135100.028.2053014
CAACGCA137650.027.6827935
AACGCAG142250.026.8791736
GGACCGA4500.023.2210856
ACGCAGA165650.022.9685367
CGCAGAG165850.022.8835568
GTACATG86650.021.8270971
GCAGAGT175050.021.2467219
AGTACTT109500.021.10381512-13
GAGTACT107850.021.09636312-13
TACATGG88400.020.6390972
AGAGTAC155150.020.26741810-11
GTACTTT113700.019.92646414-15
ACATGGG89300.019.4733813
AGGACCG5900.019.3272915
TGGTATC34550.019.1151852
Produced by FastQC (version 0.11.5)