##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180931 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.530566991803042 31.0 31.0 34.0 30.0 34.0 2 31.840805600910805 31.0 31.0 34.0 30.0 34.0 3 31.93324593946347 31.0 31.0 34.0 30.0 34.0 4 35.727990018941455 37.0 35.0 37.0 33.0 37.0 5 35.60344137434885 37.0 35.0 37.0 33.0 37.0 6 35.653418196174016 37.0 35.0 37.0 33.0 37.0 7 35.57917329800897 37.0 35.0 37.0 33.0 37.0 8 35.54032520973841 37.0 35.0 37.0 33.0 37.0 9 37.132558526610886 39.0 37.0 39.0 33.0 39.0 10-11 37.01216407121546 39.0 37.0 39.0 33.0 39.0 12-13 36.86763749059461 39.0 37.0 39.0 33.0 39.0 14-15 37.880310518764695 40.0 37.0 41.0 33.0 41.0 16-17 37.80245798697896 39.0 37.0 41.0 33.0 41.0 18-19 37.7520386935671 39.0 37.0 41.0 32.5 41.0 20-21 37.61452150480689 39.0 37.0 40.0 32.0 41.0 22-23 37.54777730244561 39.0 36.5 40.5 32.0 41.0 24-25 37.576047797935836 39.0 36.5 41.0 32.0 41.0 26-27 37.378803364251326 39.0 36.0 40.5 32.0 41.0 28-29 37.178954308962545 39.0 36.0 40.0 31.0 41.0 30-31 37.196393879494586 39.0 36.0 40.0 31.0 41.0 32-33 37.085558873710355 39.0 36.0 40.0 31.0 41.0 34-35 36.886766706512034 39.0 35.0 40.0 31.0 41.0 36-37 36.677757572339516 39.0 35.0 40.0 30.0 41.0 38-39 36.49756961591174 38.0 35.0 40.0 30.0 41.0 40-41 36.25251486635753 38.0 35.0 40.0 30.0 41.0 42-43 35.974204594276486 38.0 35.0 40.0 29.5 41.0 44-45 35.60480340735218 38.0 35.0 40.0 28.5 41.0 46-47 35.181577959137634 38.0 34.0 40.0 27.0 41.0 48-49 34.78575227735311 38.0 34.0 40.0 26.0 41.0 50-51 34.439268367499935 38.0 33.0 40.0 24.0 41.0 52-53 34.12049372492757 38.0 33.0 40.0 24.0 41.0 54-55 33.809659269367074 37.0 33.0 40.0 23.0 41.0 56-57 33.442669437960205 37.0 32.0 39.5 22.5 40.5 58-59 33.099288331451106 36.5 32.0 39.0 22.0 40.0 60-61 32.66587503226833 36.0 31.0 39.0 20.0 40.0 62-63 32.170575318522225 35.5 31.0 39.0 18.5 40.0 64-65 31.64541037749475 35.0 30.0 38.0 17.0 40.0 66-67 31.107466260968366 34.5 29.0 38.0 13.0 40.0 68-69 30.562965082343275 34.0 29.0 37.5 8.5 39.5 70-71 30.077274567604384 34.0 28.0 37.0 8.0 39.0 72-73 29.46596384754951 34.0 28.0 36.0 7.0 39.0 74-75 28.877832219359533 33.0 26.5 36.0 6.0 38.5 76-77 27.90213995766028 31.5 26.0 34.5 2.0 36.5 78-79 28.49628025829336 33.0 27.0 35.0 2.0 37.0 80-81 28.248985181099265 33.0 26.5 35.0 2.0 37.0 82-83 27.754125417081053 32.5 26.0 35.0 2.0 36.0 84-85 27.1788965354704 32.0 26.0 35.0 2.0 36.0 86-87 26.57176178430221 32.0 25.0 34.0 2.0 35.0 88-89 25.884013065979623 31.0 23.5 34.0 2.0 35.0 90-91 25.026312157514383 31.0 20.0 34.0 2.0 35.0 92-93 24.248362281979578 30.5 17.5 34.0 2.0 35.0 94-95 23.40845262871682 30.0 8.5 34.0 2.0 35.0 96-97 22.41054256186922 29.5 2.0 34.0 2.0 35.0 98-99 20.99376688212511 29.0 2.0 34.0 2.0 35.0 100-101 18.09653767129726 23.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2492185413408059 1101 2 0.25288551985645924 1101 3 0.23234980748163636 1101 4 0.07812019645182744 1101 5 -0.0177472179082514 1101 6 0.08922565101843105 1101 7 0.03608589097068915 1101 8 0.03630344708052036 1101 9 0.05700906010377338 1101 10-11 0.12745281739433523 1101 12-13 0.1201301900064422 1101 14-15 0.14687422461874888 1101 16-17 0.08952232310848274 1101 18-19 0.1497748427059804 1101 20-21 0.13884495711022993 1101 22-23 0.05305523186081729 1101 24-25 0.1520898543860696 1101 26-27 -0.07736132634413906 1101 28-29 -0.19957419245111652 1101 30-31 0.03521845399698975 1101 32-33 0.08596487566074984 1101 34-35 0.11670193472673418 1101 36-37 0.02261860458657594 1101 38-39 0.1110336603298876 1101 40-41 0.18448622480264731 1101 42-43 0.24675051661947123 1101 44-45 0.16625347551960346 1101 46-47 -0.14791423177386065 1101 48-49 -0.028511257163934545 1101 50-51 0.010048979852726347 1101 52-53 -0.15932504244720747 1101 54-55 -0.4168971249693527 1101 56-57 -0.2963630313985348 1101 58-59 -0.358539327417553 1101 60-61 -0.22137904053727908 1101 62-63 -0.2969785978451043 1101 64-65 -0.1693865661472671 1101 66-67 -0.5429045570425153 1101 68-69 -0.3190728390782276 1101 70-71 -0.23335323547547304 1101 72-73 -0.3787858667687267 1101 74-75 -0.6406149352938648 1101 76-77 -0.45321422654408394 1101 78-79 -0.405386245722525 1101 80-81 -0.38505692381103174 1101 82-83 -0.4595846903031564 1101 84-85 -0.603162918653755 1101 86-87 -0.63342057104094 1101 88-89 -0.5734267048766171 1101 90-91 -0.6922222070908468 1101 92-93 -1.021386747708661 1101 94-95 -0.940009819767468 1101 96-97 -0.9186573966012297 1101 98-99 -1.1497783220965339 1101 100-101 -1.135685407724381 1102 1 0.31000732016021004 1102 2 0.22666396267841193 1102 3 0.15119694281984764 1102 4 0.15160080925730313 1102 5 0.10995344723437483 1102 6 0.17515387498128376 1102 7 0.21409627422515598 1102 8 0.15891733651088913 1102 9 0.23432997074664996 1102 10-11 0.18266388277443468 1102 12-13 0.3158446977654563 1102 14-15 0.34789647160168613 1102 16-17 0.3595479489687037 1102 18-19 0.29260513977545344 1102 20-21 0.3108051489737491 1102 22-23 0.23379572359970524 1102 24-25 0.22175972345457495 1102 26-27 0.224110711526464 1102 28-29 0.14090741201349033 1102 30-31 0.2747710518042652 1102 32-33 0.36706682826195447 1102 34-35 0.40168099395751256 1102 36-37 0.23104608277450467 1102 38-39 0.42334444763192636 1102 40-41 0.4527414659020792 1102 42-43 0.5324782120420437 1102 44-45 0.41705296839265316 1102 46-47 0.2779766923667992 1102 48-49 0.08711782244643729 1102 50-51 0.28985210265601324 1102 52-53 0.3347975496596831 1102 54-55 0.11524346689534326 1102 56-57 0.2690021103430098 1102 58-59 0.14510232477665141 1102 60-61 0.023834155100900034 1102 62-63 0.09211285631262456 1102 64-65 0.0015408935808878255 1102 66-67 -0.031779127298943166 1102 68-69 0.043755800303415526 1102 70-71 -0.2928549064543411 1102 72-73 -0.1559260746701021 1102 74-75 -0.12118352960412082 1102 76-77 -0.06626032208006549 1102 78-79 0.16980872642536582 1102 80-81 0.41011377742415434 1102 82-83 0.34355444988480954 1102 84-85 0.2328759716626898 1102 86-87 0.08906661049101317 1102 88-89 0.18579699880411127 1102 90-91 0.1231938836308295 1102 92-93 0.1513413708119593 1102 94-95 -0.21097744796691487 1102 96-97 -0.3486443603078335 1102 98-99 -0.07672486849407179 1102 100-101 -0.1442769563961619 1103 1 0.38678349847458193 1103 2 0.2757570088822696 1103 3 0.23183892099490677 1103 4 0.23227471220599227 1103 5 0.16006083056383602 1103 6 0.25846447400613215 1103 7 0.15281376900110644 1103 8 0.1794855974814027 1103 9 0.2423053596484337 1103 10-11 0.29127123708137503 1103 12-13 0.3235886320584669 1103 14-15 0.25014208920094205 1103 16-17 0.28083524290880035 1103 18-19 0.23587191479286673 1103 20-21 0.14915550885302054 1103 22-23 0.05618587335591485 1103 24-25 0.10957244112348974 1103 26-27 0.21840851161933728 1103 28-29 0.23647930079918922 1103 30-31 0.17114541330879263 1103 32-33 0.2990336845424295 1103 34-35 0.32208963105990307 1103 36-37 0.3674054976804726 1103 38-39 0.30901167540067576 1103 40-41 0.4106181782308411 1103 42-43 0.4914004607070055 1103 44-45 0.5127532818358915 1103 46-47 0.4933651588167649 1103 48-49 0.41039678692286685 1103 50-51 0.34327240681203364 1103 52-53 0.4486282322693924 1103 54-55 0.2448500666167277 1103 56-57 0.45518013448974415 1103 58-59 0.378992648667996 1103 60-61 0.33408055630823696 1103 62-63 0.26553693339644013 1103 64-65 0.23256379235033364 1103 66-67 0.37466895141013623 1103 68-69 0.49191359575268123 1103 70-71 0.45539721218972673 1103 72-73 0.2790685561634234 1103 74-75 0.3574798381692723 1103 76-77 0.21209976034389655 1103 78-79 0.39849894003112496 1103 80-81 0.4471407103240814 1103 82-83 0.49599742063010765 1103 84-85 0.6147448769331803 1103 86-87 0.40499997478170613 1103 88-89 0.2152425235313018 1103 90-91 0.4491733937306641 1103 92-93 0.34236470491922333 1103 94-95 0.18874901476816675 1103 96-97 0.24966435225310946 1103 98-99 0.5055249573708025 1103 100-101 0.0968827835620445 1104 1 0.2727861686443589 1104 2 0.25201962793329 1104 3 0.16271008173341883 1104 4 0.04686961746493523 1104 5 0.12532185036878474 1104 6 0.09145713460898719 1104 7 0.14354349641532593 1104 8 0.15720717159646824 1104 9 0.1134586016914767 1104 10-11 0.25716731464480347 1104 12-13 0.21331865219183044 1104 14-15 0.08149284527308254 1104 16-17 0.08799579579101646 1104 18-19 0.11416446973125716 1104 20-21 0.17241811362504933 1104 22-23 0.20230788012414536 1104 24-25 0.11101211956079737 1104 26-27 0.09032853503686766 1104 28-29 0.030332555693313168 1104 30-31 0.15589385332992123 1104 32-33 0.1219423490291689 1104 34-35 0.24045954238741984 1104 36-37 0.2991749849221961 1104 38-39 0.18515006730163464 1104 40-41 0.055563418331935566 1104 42-43 0.12450199855344835 1104 44-45 0.07495110286311757 1104 46-47 -0.05451351980617147 1104 48-49 0.06143433350804628 1104 50-51 0.1224084926572857 1104 52-53 0.1027759197881295 1104 54-55 -0.1755961872921361 1104 56-57 -0.1219090591760974 1104 58-59 -0.05264261613574206 1104 60-61 0.01888289948930577 1104 62-63 -0.01414817933612511 1104 64-65 -0.06990789956633492 1104 66-67 -0.02901895286356293 1104 68-69 -0.0044084952615541795 1104 70-71 0.013208172736899115 1104 72-73 0.0027252608597834183 1104 74-75 -0.1814978580596467 1104 76-77 -0.25008628052255233 1104 78-79 -0.1472205779104172 1104 80-81 -0.25852608247359754 1104 82-83 -0.15819917240460768 1104 84-85 0.06968451253353791 1104 86-87 -0.14412097534181 1104 88-89 -0.19259610254497517 1104 90-91 -0.260110622376029 1104 92-93 -0.16844287864852348 1104 94-95 -0.25739226862680553 1104 96-97 -0.3603102311575661 1104 98-99 -0.2319452352526774 1104 100-101 -0.267167200017127 1105 1 0.2528739375258944 1105 2 0.16072353176435072 1105 3 0.08998889267370558 1105 4 -0.03746625768638978 1105 5 0.032145198710466616 1105 6 0.11414941148161972 1105 7 0.04295326909551278 1105 8 0.09609453245882094 1105 9 0.12879505508654177 1105 10-11 0.11319039819272803 1105 12-13 0.20093911061422887 1105 14-15 0.12698460150977553 1105 16-17 -0.04629102609907676 1105 18-19 0.1439472709865015 1105 20-21 -0.10766481851107557 1105 22-23 -0.009129181906750716 1105 24-25 -0.02073306480944126 1105 26-27 -0.05002677805577349 1105 28-29 -0.09449139038347454 1105 30-31 -0.12009625372096622 1105 32-33 -0.21098022189592314 1105 34-35 -0.19986729165951544 1105 36-37 -0.18273955772742312 1105 38-39 -0.12711165982074135 1105 40-41 -0.0713441182558725 1105 42-43 -0.0586227772056489 1105 44-45 -0.12303746076066346 1105 46-47 -0.17990379823666558 1105 48-49 -0.2416610391704026 1105 50-51 -0.01740196467504518 1105 52-53 0.0945576035386182 1105 54-55 -0.11836377301262502 1105 56-57 -0.07960829157738658 1105 58-59 0.07630122636250292 1105 60-61 -0.14549894640042993 1105 62-63 -0.3590718345584776 1105 64-65 -0.27815469486155564 1105 66-67 -0.293551387572915 1105 68-69 -0.2653399008269872 1105 70-71 -0.6776155105674526 1105 72-73 -0.49440391417192586 1105 74-75 -0.5516014654776278 1105 76-77 -0.6626409916930527 1105 78-79 -0.4856364643811766 1105 80-81 -0.501335935573703 1105 82-83 -0.512460453391359 1105 84-85 -0.40155742652491355 1105 86-87 -0.321712550453217 1105 88-89 -0.4733536523796964 1105 90-91 -0.2835403224472799 1105 92-93 -0.34345708207625947 1105 94-95 -0.4206087837300849 1105 96-97 -0.43927948461674404 1105 98-99 -0.4464088726729152 1105 100-101 -0.561501927343798 1106 1 0.1062130662673546 1106 2 0.13342479321600464 1106 3 0.04341790066973772 1106 4 0.09141931326022501 1106 5 0.04776118581316524 1106 6 -0.029649443634340855 1106 7 0.018789055003665567 1106 8 -0.02936014911763607 1106 9 0.13938913096914973 1106 10-11 -0.082544662436014 1106 12-13 -0.2822957124182892 1106 14-15 -0.04374108134175003 1106 16-17 0.08397590015842127 1106 18-19 0.03278889924015971 1106 20-21 0.14024588643010816 1106 22-23 0.1297181680517312 1106 24-25 0.061341590443298344 1106 26-27 0.11879464385188498 1106 28-29 0.13084806187675468 1106 30-31 0.14953860065485713 1106 32-33 0.18360134304271014 1106 34-35 -0.3346792880767566 1106 36-37 0.10564726287831405 1106 38-39 0.05516915136571754 1106 40-41 -0.17693274979088613 1106 42-43 -0.2406030534224044 1106 44-45 -0.010974380807688533 1106 46-47 0.03747889614709976 1106 48-49 0.03710138905129412 1106 50-51 0.23210286541359437 1106 52-53 0.3056114135539687 1106 54-55 0.12805676724820358 1106 56-57 0.17407270417768927 1106 58-59 0.26939295634613103 1106 60-61 0.25360344418602665 1106 62-63 -0.17220667710297732 1106 64-65 -0.3954276294909782 1106 66-67 -0.41928701455345063 1106 68-69 0.15465902853536662 1106 70-71 0.0698728549761718 1106 72-73 -0.03583196635232255 1106 74-75 -0.0719811112594897 1106 76-77 -0.07904106570669711 1106 78-79 -0.11216333525257838 1106 80-81 -0.20296929803923902 1106 82-83 -0.4419198822372117 1106 84-85 -0.5912478454429255 1106 86-87 -0.46488027243499985 1106 88-89 -0.38903725257722144 1106 90-91 -0.5361179989541149 1106 92-93 -0.7222819447650402 1106 94-95 -0.632435411807247 1106 96-97 -0.6561097920410326 1106 98-99 -0.5963023131941441 1106 100-101 -0.9458820805148065 1107 1 0.1724397004929763 1107 2 0.07668421845846396 1107 3 0.09814185914615692 1107 4 0.035172242131601195 1107 5 0.09643629081457306 1107 6 -0.02137952521871256 1107 7 0.05803603097508159 1107 8 -0.00444289408873999 1107 9 0.047930108094391244 1107 10-11 0.07132505101336051 1107 12-13 0.10151802549644628 1107 14-15 -0.007141984260293555 1107 16-17 0.007712767766072659 1107 18-19 0.05360274823800637 1107 20-21 0.11336626269527272 1107 22-23 0.12997487354970616 1107 24-25 0.07737836810945709 1107 26-27 0.05702895196935032 1107 28-29 0.07734323055785097 1107 30-31 0.1856202057301246 1107 32-33 0.11244226048566475 1107 34-35 -0.014952540578221374 1107 36-37 0.09426233920515159 1107 38-39 0.10421463293089772 1107 40-41 0.04742556609364357 1107 42-43 0.19319897896050264 1107 44-45 0.05878431801495054 1107 46-47 -0.06480475755548554 1107 48-49 0.011495239585990191 1107 50-51 0.09743311071615324 1107 52-53 0.05871089106521765 1107 54-55 -0.1426361444948725 1107 56-57 -0.12935190045204337 1107 58-59 0.037347410679238635 1107 60-61 0.02584504954796074 1107 62-63 0.032738571665454685 1107 64-65 0.03953798849540746 1107 66-67 0.056932341538782794 1107 68-69 0.191376574450711 1107 70-71 0.10997325977256978 1107 72-73 0.05989380250005283 1107 74-75 0.03274816248393009 1107 76-77 0.14305059736668824 1107 78-79 0.12755659994496327 1107 80-81 0.001568247852215876 1107 82-83 -0.25191710316876836 1107 84-85 -0.09750841376640196 1107 86-87 -0.15984078219100084 1107 88-89 -0.04537547953951915 1107 90-91 0.021678163940702433 1107 92-93 -0.12137070494616253 1107 94-95 -0.15388231507695238 1107 96-97 -0.10977619155083218 1107 98-99 -0.11965206214233959 1107 100-101 0.05739606561562027 1108 1 0.12627324601517387 1108 2 0.07075523099913283 1108 3 0.011061393437721279 1108 4 -0.03361808144104117 1108 5 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0.28348624852100457 2316 100-101 0.6555612066654852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 17.0 5 89.0 6 197.0 7 371.0 8 985.0 9 1761.0 10 2905.0 11 4692.0 12 6649.0 13 9420.0 14 12537.0 15 16718.0 16 21702.0 17 27552.0 18 25364.0 19 19348.0 20 18707.0 21 19561.0 22 21666.0 23 24564.0 24 28441.0 25 33906.0 26 40730.0 27 49487.0 28 60425.0 29 72248.0 30 87811.0 31 106213.0 32 129619.0 33 161154.0 34 204377.0 35 261041.0 36 316692.0 37 291584.0 38 99674.0 39 2705.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.100374529430624 21.11773669943589 16.033271530856375 21.748617240277117 2 16.591354792976027 21.602884272817434 38.674400978297804 23.131359955908735 3 20.24314386837548 24.809175531000292 30.214940316772974 24.732740283851253 4 13.86261824084221 17.832851123144714 36.407633440779854 31.896897195233226 5 13.487343789776416 37.79075340973463 34.206329366489996 14.51557343399896 6 31.896745013941292 37.09278285282753 16.656556305541073 14.3539158276901 7 28.178974942352603 33.17583178926798 20.30362262721746 18.34157064116196 8 26.318393383376183 35.97537015155454 19.046774061169288 18.659462403899983 9 25.89233680478658 16.963306037650895 20.345944002813475 36.79841315474905 10-11 25.087382406871196 27.387386395993268 26.55946015715307 20.965771039982467 12-13 25.73838617689905 24.44374288988207 27.866366236242307 21.951504696976578 14-15 22.514994743070734 25.880919662291014 26.323299545010826 25.28078604962743 16-17 21.63367387597315 29.283411533881633 27.040951777016332 22.041962813128887 18-19 21.563038904027685 27.33206598466435 29.94817350938659 21.15672160192138 20-21 22.18563998585925 26.342213485892035 30.430123649028783 21.042022879219928 22-23 21.96455579192364 26.222964110796337 30.340583253722453 21.471896843557573 24-25 21.851859748597672 26.491493408879208 30.368446621195165 21.288200221327955 26-27 21.526222455560358 26.75684319486269 30.32088706192262 21.396047287654337 28-29 21.50932261851786 26.90017976478119 30.360783628634984 21.229713988065964 30-31 21.668636009117208 26.61769216907825 30.34206492548366 21.371606896320884 32-33 21.46608489677115 26.6203057318182 30.288876631126797 21.62473274028385 34-35 21.978526601712755 26.621979329011324 30.1629670998303 21.236526969445617 36-37 21.96380811680883 26.478600194137275 30.056773919028156 21.500817770025737 38-39 21.655751603329037 26.768797362227414 30.151504105356842 21.423946929086707 40-41 21.95728542813498 26.641183519695943 29.97280732865534 21.42872372351374 42-43 21.740940768348032 26.668974775503933 29.832268507956673 21.757815948191364 44-45 21.887258239715056 26.904977736572132 29.67225923241038 21.53550479130243 46-47 22.145175615367933 27.171538210058 29.042482316038427 21.640803858535644 48-49 22.117114204896897 27.22477235639275 28.94082848104777 21.717284957662574 50-51 22.289242529910393 27.41331568949224 28.68905527043267 21.608386510164696 52-53 22.414876949339522 27.484065291382443 28.468484330774334 21.632573428503697 54-55 22.19242350392883 27.46533701922438 28.559022324889987 21.783217151956805 56-57 22.227479915687383 27.741203183411123 28.359287845420145 21.67202905548135 58-59 22.367603560130974 27.29414639894614 28.548450180221195 21.78979986070169 60-61 22.162393033067072 27.512952037455563 28.726493410382997 21.59816151909437 62-63 22.069611555798875 27.52205365506749 28.785802943788685 21.622531845344948 64-65 22.092084089795616 27.346011255287593 28.69339486058817 21.868509794328613 66-67 22.017088115121478 27.53273716591676 28.52692267660004 21.923252042361728 68-69 22.064384430318977 27.436356308383896 28.605375410776407 21.89388385052072 70-71 22.13284143331449 27.445136962150567 28.49622477740011 21.925796827134832 72-73 22.157005425664543 27.497866736728486 28.306924886665374 22.038202950941592 74-75 22.091653560636974 27.83225504715878 28.038497338291464 22.03759405391278 76-77 22.16232425510023 27.578726699744283 28.27925321800644 21.979695827149047 78-79 22.196850794454296 27.57854329183271 28.223428434920685 22.001177478792314 80-81 22.108127217229708 27.840174677694986 28.140321724988087 21.91137638008722 82-83 22.29971755039158 27.788983548227353 28.022302514535152 21.888996386845918 84-85 22.22363853952213 27.780213028386974 27.9876016194926 22.008546812598297 86-87 22.231400259797308 27.734417090682832 28.02695270964556 22.0072299398743 88-89 22.18683213728449 27.742234852913732 27.948683383380768 22.12224962642101 90-91 22.266249979937587 27.727926188669212 27.945769582633062 22.060054248760146 92-93 22.290771824966956 27.601921681505708 27.991851997051683 22.115454496475657 94-95 22.120942845051037 27.619259848202443 27.97628627407286 22.283511032673662 96-97 22.209093272551954 27.484065291382443 27.933277118808437 22.373564317257173 98-99 22.096622140869766 27.53201877383182 27.746516588503507 22.624842496794916 100-101 22.46032049943008 27.20436510620754 28.098743192992824 22.236571201369554 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 658.0 1 486.0 2 446.5 3 614.0 4 501.0 5 402.0 6 826.0 7 1921.0 8 3026.0 9 3481.0 10 3174.0 11 2718.0 12 2705.5 13 2924.5 14 3253.5 15 3554.5 16 3754.0 17 3950.5 18 4268.0 19 4580.5 20 4964.0 21 5658.0 22 6554.0 23 7870.0 24 9585.0 25 11778.0 26 14604.0 27 17867.0 28 20885.5 29 24020.0 30 27932.5 31 31966.5 32 36536.5 33 40430.0 34 44445.0 35 48778.0 36 53528.0 37 58424.0 38 61830.5 39 64095.5 40 66071.0 41 69668.5 42 72919.5 43 75473.0 44 78580.0 45 81037.0 46 81951.5 47 83506.0 48 85161.0 49 82990.0 50 79154.5 51 75497.5 52 72037.0 53 69729.0 54 68118.0 55 63087.0 56 55568.0 57 49561.0 58 44815.0 59 39817.5 60 33366.0 61 27074.0 62 21682.0 63 16404.5 64 12111.5 65 9404.5 66 7052.5 67 5263.0 68 3953.5 69 2828.0 70 2043.5 71 1499.0 72 1142.5 73 949.0 74 788.5 75 629.0 76 459.5 77 295.5 78 185.0 79 132.5 80 93.5 81 59.0 82 39.0 83 27.0 84 14.0 85 8.5 86 6.5 87 4.5 88 3.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015039448749180968 2 0.0 3 0.0 4 4.585197789384442E-5 5 0.035581134845623266 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002751118673630665 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.814457678853664E-4 24-25 2.0633390052229988E-4 26-27 0.0010775214805053438 28-29 0.0011692254362930326 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.731497236730552E-4 42-43 0.009789397280335782 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 9.170395578768883E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.292598894692221E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.585197789384442E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.043717568322887E-4 84-85 4.585197789384442E-5 86-87 0.0 88-89 0.0 90-91 0.010821066782947282 92-93 0.0013067813699745659 94-95 0.0 96-97 0.0 98-99 0.0010545954915584215 100-101 0.016758897920200133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180931.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.43962004450436 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19358724146873 44.91409941066767 2 12.51952742280629 14.38233796605492 3 3.966183497940485 6.834482194454542 4 1.7058615043823444 3.9193614664107383 5 0.9324844618556115 2.678077659319522 6 0.5484278275814791 1.8900891622867864 7 0.3771412483532101 1.516399500596291 8 0.27251993698289284 1.2522753307879708 9 0.20608844961348774 1.0653878017223746 >10 1.1561801699427008 12.23546830999966 >50 0.08312450922397266 3.2809321435296432 >100 0.03639606358861372 3.7174406787204797 >500 0.0019981179517567487 0.8097333183756671 >1k 2.3977415421079276E-4 0.258877774255593 >5k 1.5984943614052853E-4 0.743762869429839 >10k+ 7.992471807026426E-5 0.5012744133883349 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10919 0.5006577466228872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9525 0.43674008943886805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6676 0.30610780441930535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.11385046111041569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.585197789384442E-5 2 0.0 0.0 0.0 0.0 4.585197789384442E-5 3 0.0 0.0 0.0 0.0 4.585197789384442E-5 4 0.0 0.0 0.0 0.0 4.585197789384442E-5 5 0.0 0.0 0.0 0.0 4.585197789384442E-5 6 0.0 0.0 0.0 0.0 4.585197789384442E-5 7 0.0 0.0 0.0 0.0 4.585197789384442E-5 8 0.0 0.0 0.0 0.0 4.585197789384442E-5 9 0.0 0.0 0.0 4.585197789384442E-5 4.585197789384442E-5 10-11 0.0 0.0 0.0 4.585197789384442E-5 4.585197789384442E-5 12-13 0.0 2.292598894692221E-5 0.0 4.585197789384442E-5 9.170395578768883E-5 14-15 0.0 4.585197789384442E-5 0.0 4.585197789384442E-5 9.170395578768883E-5 16-17 0.0 4.585197789384442E-5 0.0 4.585197789384442E-5 9.170395578768883E-5 18-19 0.0 4.585197789384442E-5 0.0 6.877796684076662E-5 9.170395578768883E-5 20-21 0.0 4.585197789384442E-5 0.0 9.170395578768883E-5 9.170395578768883E-5 22-23 0.0 4.585197789384442E-5 0.0 9.170395578768883E-5 9.170395578768883E-5 24-25 0.0 4.585197789384442E-5 0.0 1.3755593368153325E-4 1.3755593368153325E-4 26-27 0.0 4.585197789384442E-5 0.0 1.8340791157537767E-4 1.3755593368153325E-4 28-29 0.0 4.585197789384442E-5 0.0 5.731497236730552E-4 1.3755593368153325E-4 30-31 0.0 4.585197789384442E-5 0.0 0.0013984853257622547 1.3755593368153325E-4 32-33 0.0 4.585197789384442E-5 0.0 0.003278416419409876 1.3755593368153325E-4 34-35 0.0 4.585197789384442E-5 0.0 0.005777349214624397 1.3755593368153325E-4 36-37 0.0 4.585197789384442E-5 0.0 0.009743545302441939 1.3755593368153325E-4 38-39 0.0 4.585197789384442E-5 0.0 0.016827675887040903 1.3755593368153325E-4 40-41 0.0 4.585197789384442E-5 0.0 0.02524151383056135 1.3755593368153325E-4 42-43 0.0 4.585197789384442E-5 0.0 0.03409094556407333 1.3755593368153325E-4 44-45 0.0 4.585197789384442E-5 0.0 0.04470567844649831 1.3755593368153325E-4 46-47 0.0 4.585197789384442E-5 0.0 0.05775056615729704 1.3755593368153325E-4 48-49 0.0 4.585197789384442E-5 0.0 0.07189590133754806 1.3755593368153325E-4 50-51 0.0 4.585197789384442E-5 0.0 0.0895030608487843 1.3755593368153325E-4 52-53 0.0 4.585197789384442E-5 0.0 0.1084857796968359 1.8340791157537767E-4 54-55 0.0 9.170395578768883E-5 0.0 0.13120543474323582 2.292598894692221E-4 56-57 0.0 9.170395578768883E-5 0.0 0.16066533054003085 2.292598894692221E-4 58-59 0.0 9.170395578768883E-5 0.0 0.19276171506572193 2.292598894692221E-4 60-61 0.0 9.170395578768883E-5 0.0 0.2311168945739228 2.292598894692221E-4 62-63 0.0 9.170395578768883E-5 0.0 0.26951792606001745 4.1266780104459976E-4 64-65 0.0 1.3755593368153325E-4 0.0 0.31298560110338197 4.1266780104459976E-4 66-67 0.0 2.292598894692221E-4 0.0 0.36587585760393154 4.1266780104459976E-4 68-69 0.0 2.292598894692221E-4 0.0 0.4290369571527022 4.1266780104459976E-4 70-71 0.0 2.292598894692221E-4 0.0 0.4987778155292396 4.1266780104459976E-4 72-73 0.0 2.292598894692221E-4 0.0 0.5750525807556498 4.8144576788536635E-4 74-75 0.0 2.292598894692221E-4 0.0 0.6571276211856313 5.50223734726133E-4 76-77 0.0 2.292598894692221E-4 0.0 0.7508490640006493 5.50223734726133E-4 78-79 0.0 2.292598894692221E-4 0.0 0.8608021069900882 5.50223734726133E-4 80-81 4.585197789384442E-5 2.292598894692221E-4 0.0 0.9882247535570818 5.50223734726133E-4 82-83 4.585197789384442E-5 2.292598894692221E-4 0.0 1.1204160058250352 5.50223734726133E-4 84-85 4.585197789384442E-5 2.292598894692221E-4 0.0 1.2535472236398126 5.50223734726133E-4 86-87 4.585197789384442E-5 2.751118673630665E-4 0.0 1.397522434226484 5.731497236730552E-4 88-89 4.585197789384442E-5 2.751118673630665E-4 0.0 1.566693306665823 5.960757126199774E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6285 0.0 44.760807 1 GTATCAA 10415 0.0 36.86664 1 ATCAACG 13245 0.0 28.769623 3 TATCAAC 13340 0.0 28.600357 2 TCAACGC 13510 0.0 28.205301 4 CAACGCA 13765 0.0 27.682793 5 AACGCAG 14225 0.0 26.879173 6 GGACCGA 450 0.0 23.221085 6 ACGCAGA 16565 0.0 22.968536 7 CGCAGAG 16585 0.0 22.883556 8 GTACATG 8665 0.0 21.827097 1 GCAGAGT 17505 0.0 21.246721 9 AGTACTT 10950 0.0 21.103815 12-13 GAGTACT 10785 0.0 21.096363 12-13 TACATGG 8840 0.0 20.639097 2 AGAGTAC 15515 0.0 20.267418 10-11 GTACTTT 11370 0.0 19.926464 14-15 ACATGGG 8930 0.0 19.473381 3 AGGACCG 590 0.0 19.327291 5 TGGTATC 3455 0.0 19.115185 2 >>END_MODULE