FastQCFastQC Report
Thu 26 May 2016
SRR937526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169198
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101930.4698971693685869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.3644203986911292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.24290083247356856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27890.12857286425674372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.045.6356661
GTATCAA88150.039.2178881
ATCAACG110600.030.8582133
TATCAAC111700.030.8522132
TCAACGC111550.030.5968284
CAACGCA113250.030.0116145
AACGCAG117600.028.8828036
GTACATG85750.026.0082341
TACATGG84950.025.4594762
ACGCAGA134800.024.9865887
CGCAGAG135400.024.8056958
ACATGGG87400.023.9843883
GCAGAGT144700.022.521449
GAGTACT83600.022.32989912-13
GTACTTT90100.020.5864614-15
AGTACTT89050.020.37651412-13
AGAGTAC127700.020.2910410-11
CATGGGG40850.019.4332734
GTACCTG16400.019.1369291
GAGTACA65650.019.1223531
Produced by FastQC (version 0.11.5)