##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2169198 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866978947979852 33.0 31.0 34.0 30.0 34.0 2 32.009173436449785 33.0 31.0 34.0 30.0 34.0 3 32.06319939443057 34.0 31.0 34.0 30.0 34.0 4 35.652603404576254 37.0 35.0 37.0 35.0 37.0 5 35.64046896594963 37.0 35.0 37.0 35.0 37.0 6 35.729562723181566 37.0 35.0 37.0 35.0 37.0 7 35.68116557363597 37.0 35.0 37.0 35.0 37.0 8 35.664348759311046 37.0 35.0 37.0 33.0 37.0 9 37.3109190585645 39.0 37.0 39.0 35.0 39.0 10-11 37.210074184099376 39.0 37.0 39.0 34.0 39.0 12-13 37.089906961005866 39.0 37.0 39.0 34.0 39.0 14-15 38.26699476027546 40.0 38.0 41.0 33.5 41.0 16-17 38.1967830967943 40.0 38.0 41.0 33.0 41.0 18-19 38.141954999036514 40.0 38.0 41.0 33.5 41.0 20-21 37.97133710246828 40.0 38.0 41.0 33.0 41.0 22-23 37.82540851503643 40.0 38.0 41.0 33.0 41.0 24-25 37.67898734924152 40.0 37.0 41.0 33.0 41.0 26-27 37.78503253276095 40.0 38.0 41.0 33.0 41.0 28-29 37.72504008393886 40.0 38.0 41.0 33.0 41.0 30-31 37.51967593553009 40.0 37.0 41.0 32.5 41.0 32-33 37.28352921217888 39.5 36.5 41.0 32.0 41.0 34-35 37.02399250783009 39.0 36.0 41.0 31.0 41.0 36-37 37.039279494080304 39.0 36.0 41.0 31.0 41.0 38-39 36.988632204160254 39.5 36.0 41.0 31.0 41.0 40-41 36.79828005557815 39.0 36.0 41.0 31.0 41.0 42-43 36.55770773345725 39.0 36.0 41.0 30.0 41.0 44-45 36.32667810868349 39.0 35.5 41.0 30.0 41.0 46-47 35.97725587982286 39.0 35.0 40.5 29.5 41.0 48-49 35.637322180824434 39.0 35.0 40.0 27.5 41.0 50-51 34.749711414080224 38.0 34.0 39.5 26.5 40.5 52-53 34.86522853146647 38.0 34.5 39.5 27.0 40.5 54-55 35.16230330287968 39.0 35.0 40.0 27.0 41.0 56-57 34.88359292236117 38.0 34.5 40.0 26.0 41.0 58-59 34.65194786275849 38.0 34.0 40.0 26.0 41.0 60-61 34.343667567460415 38.0 34.0 40.0 25.0 41.0 62-63 33.97346231187748 37.5 34.0 40.0 23.5 41.0 64-65 33.596271064236646 37.0 33.0 40.0 22.5 41.0 66-67 33.17238260407763 36.0 33.0 39.0 21.5 41.0 68-69 32.678608407346864 36.0 32.0 39.0 20.0 40.0 70-71 32.22246563015456 35.0 31.5 38.5 18.5 40.0 72-73 31.74918172522748 35.0 31.0 38.0 16.5 40.0 74-75 31.24382467621674 35.0 31.0 37.0 12.0 39.0 76-77 30.767242317206637 35.0 30.5 36.5 7.5 39.0 78-79 30.223601072838903 34.0 30.0 36.0 6.5 38.5 80-81 29.69157725574152 34.0 29.0 35.5 2.0 37.5 82-83 29.166301554768168 34.0 29.0 35.0 2.0 37.0 84-85 28.68051072331802 34.0 28.5 35.0 2.0 36.5 86-87 28.137520871769198 33.5 27.5 35.0 2.0 36.0 88-89 27.630457431732836 33.0 26.5 35.0 2.0 35.5 90-91 27.098864649515626 33.0 25.5 35.0 2.0 35.0 92-93 26.51400241010733 32.5 25.0 35.0 2.0 35.0 94-95 25.847993359757844 32.0 23.5 34.5 2.0 35.0 96-97 25.04173547089754 32.0 19.0 34.0 2.0 35.0 98-99 24.065356412830916 31.5 4.5 34.0 2.0 35.0 100-101 21.486944253129497 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14946155130547112 1101 2 0.21014713832714804 1101 3 0.25916759716707816 1101 4 0.1554010680171345 1101 5 0.18175043918160583 1101 6 0.08472660543004906 1101 7 0.17294246497583288 1101 8 0.10668226288618143 1101 9 0.16018013449848922 1101 10-11 0.0038025482941961286 1101 12-13 0.17045660338244062 1101 14-15 0.24428954126850755 1101 16-17 0.32767714121418123 1101 18-19 0.32649636493130885 1101 20-21 0.41922941450300755 1101 22-23 0.27834753153694436 1101 24-25 0.3266343529123361 1101 26-27 0.3993952282023443 1101 28-29 0.3264648245685109 1101 30-31 0.4095520965995476 1101 32-33 0.2876272735300134 1101 34-35 0.44010942889645577 1101 36-37 0.39269612758466366 1101 38-39 0.30362319186294684 1101 40-41 0.31698918940131193 1101 42-43 0.3436269196766659 1101 44-45 0.3971979986678633 1101 46-47 0.35882270468788846 1101 48-49 0.39448523785717526 1101 50-51 0.4355421955815686 1101 52-53 0.2741849364464102 1101 54-55 0.3581250987972808 1101 56-57 0.5564107189076211 1101 58-59 0.521295458115425 1101 60-61 0.5625751880090277 1101 62-63 0.5584983704708435 1101 64-65 0.5903606400166908 1101 66-67 0.7235046412039878 1101 68-69 0.6842557689097504 1101 70-71 0.7220350030537119 1101 72-73 0.5541787173229196 1101 74-75 0.553064554242475 1101 76-77 0.4888349361603801 1101 78-79 0.5718004569522925 1101 80-81 0.6602639517938762 1101 82-83 0.7797262127489795 1101 84-85 0.8726226706589557 1101 86-87 1.0314575281792209 1101 88-89 1.112179334521059 1101 90-91 1.1154540771970858 1101 92-93 1.49364765183876 1101 94-95 1.3280753312726965 1101 96-97 1.492584526441366 1101 98-99 1.6350199655501498 1101 100-101 1.8888501642918456 1102 1 0.11716450149734214 1102 2 0.18490969873460372 1102 3 0.14017058693586648 1102 4 0.09593617257128528 1102 5 0.058971068674047444 1102 6 0.050001059536178616 1102 7 0.048976839857914456 1102 8 0.061402929673946005 1102 9 0.12613257374440678 1102 10-11 0.07436732237666632 1102 12-13 0.1614424548522706 1102 14-15 0.3226829113004399 1102 16-17 0.354106627161741 1102 18-19 0.24044350034316864 1102 20-21 0.3253523416507562 1102 22-23 0.391066553820238 1102 24-25 0.2725266457675204 1102 26-27 0.29193045912183635 1102 28-29 0.27704228783699847 1102 30-31 0.302273628638865 1102 32-33 0.22253411858241634 1102 34-35 0.3209349806698256 1102 36-37 0.3692596573195672 1102 38-39 0.3797296125384193 1102 40-41 0.3416617583823154 1102 42-43 0.5385938058153599 1102 44-45 0.4029692089582113 1102 46-47 0.43731593843831007 1102 48-49 0.4814915464711689 1102 50-51 0.4267985039761797 1102 52-53 0.40395073057796793 1102 54-55 0.5887460528029393 1102 56-57 0.6489366479341783 1102 58-59 0.547736790813417 1102 60-61 0.5596441264436649 1102 62-63 0.5819872296989743 1102 64-65 0.3996231315374885 1102 66-67 0.4287149297995967 1102 68-69 0.5619458807342994 1102 70-71 0.5696792986678716 1102 72-73 0.4460456160103874 1102 74-75 0.45185601019055355 1102 76-77 0.5592637797508075 1102 78-79 0.6399972850067996 1102 80-81 0.7237284856944477 1102 82-83 0.8783549733243952 1102 84-85 0.9670272310435948 1102 86-87 1.0178895765350227 1102 88-89 0.9597177114109421 1102 90-91 1.149299628917806 1102 92-93 1.3510601635867907 1102 94-95 1.2503574892767553 1102 96-97 1.3418595491915148 1102 98-99 1.6879265877081373 1102 100-101 1.8806074585993535 1103 1 0.16280673528130407 1103 2 0.17906684089525626 1103 3 0.09028777144313693 1103 4 0.0797835158087139 1103 5 0.0895314996622929 1103 6 0.14033168352191439 1103 7 0.12689826877758748 1103 8 0.002325222241701397 1103 9 0.09102162282608361 1103 10-11 0.18786164946247652 1103 12-13 0.1680662525357448 1103 14-15 0.276951962053694 1103 16-17 0.15734365328709288 1103 18-19 0.13572451680123976 1103 20-21 0.06639921893611955 1103 22-23 0.0781437841565733 1103 24-25 0.10330490412366089 1103 26-27 -0.08619800156476032 1103 28-29 0.04049952680981761 1103 30-31 0.009305395565320396 1103 32-33 0.033806293095047124 1103 34-35 -0.0907886066735557 1103 36-37 -0.06893970608822286 1103 38-39 0.034804415605769634 1103 40-41 0.07710780823709484 1103 42-43 0.0736492986867816 1103 44-45 -0.06797251569127383 1103 46-47 -0.06672971302396746 1103 48-49 0.023410603841078625 1103 50-51 -0.16976092058346381 1103 52-53 -0.08787247230271333 1103 54-55 0.004035757978556376 1103 56-57 0.2989551049293837 1103 58-59 0.29489502298152104 1103 60-61 0.30752131081163014 1103 62-63 0.15210326499710902 1103 64-65 0.32009176509300374 1103 66-67 0.3969780394898663 1103 68-69 0.3040099822268729 1103 70-71 0.3660340421156363 1103 72-73 0.296397517777379 1103 74-75 0.30791974254322696 1103 76-77 0.2458445865652692 1103 78-79 0.3189101679894506 1103 80-81 0.48377979962355866 1103 82-83 0.292925726300151 1103 84-85 0.4748919134091558 1103 86-87 0.4695629817461615 1103 88-89 0.30770450595324306 1103 90-91 0.4296451926222993 1103 92-93 0.5793361931736989 1103 94-95 0.5822057309867752 1103 96-97 0.7174186568480287 1103 98-99 0.952403314481927 1103 100-101 1.120216257362383 1104 1 0.17923801136062067 1104 2 0.22030788522504352 1104 3 0.3169889131124677 1104 4 0.16285567840004944 1104 5 0.18584477489675777 1104 6 0.1970931012607977 1104 7 0.22246532529503327 1104 8 0.2646819210621061 1104 9 0.39337142757539567 1104 10-11 0.34253334110998424 1104 12-13 0.33621484242285504 1104 14-15 0.45262033492745246 1104 16-17 0.4563181340708269 1104 18-19 0.39432929692289775 1104 20-21 0.5406749755063984 1104 22-23 0.5118776625560386 1104 24-25 0.42689859029773913 1104 26-27 0.4820829279986114 1104 28-29 0.4146691362944992 1104 30-31 0.4053394574349056 1104 32-33 0.487534633234155 1104 34-35 0.43807561079709245 1104 36-37 0.3918174148807694 1104 38-39 0.501701546863579 1104 40-41 0.4831730398123568 1104 42-43 0.4817905936847069 1104 44-45 0.44446887773363386 1104 46-47 0.24313220688770087 1104 48-49 0.41337532139328914 1104 50-51 0.34887389649710343 1104 52-53 0.4696264507165182 1104 54-55 0.5704819863152082 1104 56-57 0.6541914621273079 1104 58-59 0.6437645591339205 1104 60-61 0.6545869913031552 1104 62-63 0.7053852332021293 1104 64-65 0.6530490845889361 1104 66-67 0.6734164361896404 1104 68-69 0.7843129972153804 1104 70-71 0.9748455223821395 1104 72-73 0.8819814156252335 1104 74-75 0.8598947823810619 1104 76-77 0.8150872181346784 1104 78-79 0.7223351411172132 1104 80-81 0.9479451813487643 1104 82-83 1.0909282232225657 1104 84-85 1.0707898191172234 1104 86-87 0.9231741261619639 1104 88-89 0.9641804017990978 1104 90-91 1.2298403387501189 1104 92-93 1.30560453844328 1104 94-95 1.259659225632543 1104 96-97 1.3417374403686217 1104 98-99 1.3773713884499372 1104 100-101 1.4622919269377128 1105 1 -0.027429379360917494 1105 2 0.07331873269759015 1105 3 0.14041267753147224 1105 4 0.05051092773170751 1105 5 0.13572568470898716 1105 6 0.1488187272826096 1105 7 0.15043010447573835 1105 8 0.11372802687971983 1105 9 0.1396710802094674 1105 10-11 0.1719248752246969 1105 12-13 0.19593802133164928 1105 14-15 0.15465022636612247 1105 16-17 0.1987178130811884 1105 18-19 0.1744248721658508 1105 20-21 0.35054932191757615 1105 22-23 0.3257568923685241 1105 24-25 0.2609686675033984 1105 26-27 0.281368693572972 1105 28-29 0.21425682192526807 1105 30-31 0.27663870414120595 1105 32-33 0.3323314871315475 1105 34-35 0.3020444333792369 1105 36-37 0.299731076028138 1105 38-39 0.345956622494235 1105 40-41 0.453239925884489 1105 42-43 0.3718136648151571 1105 44-45 0.4363374228055008 1105 46-47 0.42008676715246196 1105 48-49 0.40637613433130326 1105 50-51 0.38187889139323516 1105 52-53 0.37360017690614455 1105 54-55 0.4305618404265701 1105 56-57 0.5542055884137724 1105 58-59 0.6822415506065482 1105 60-61 0.6688835870304857 1105 62-63 0.7658627882829236 1105 64-65 0.6650850234493575 1105 66-67 0.674479696334501 1105 68-69 0.5938997663514485 1105 70-71 0.6885863398448961 1105 72-73 0.5895893860982255 1105 74-75 0.5479495728880508 1105 76-77 0.5992482953262623 1105 78-79 0.5703402677143572 1105 80-81 0.7322613229493626 1105 82-83 0.7050233344449701 1105 84-85 0.7661907296222381 1105 86-87 0.9197149900989459 1105 88-89 0.8287935670651017 1105 90-91 0.795104052884593 1105 92-93 0.8360290955941245 1105 94-95 1.0564185869584186 1105 96-97 0.946079640728076 1105 98-99 1.407481757505039 1105 100-101 1.6414826079906994 1106 1 0.08288853014231634 1106 2 0.184281526121719 1106 3 0.18084561660062803 1106 4 0.023641541798035348 1106 5 0.09826294337313612 1106 6 0.04932701553755692 1106 7 -0.06806892917018104 1106 8 0.03034364185564442 1106 9 0.11067657046896073 1106 10-11 -0.14933188264568287 1106 12-13 -0.2028229774571102 1106 14-15 0.012188410180193898 1106 16-17 -0.002784506013128407 1106 18-19 0.03266877433940607 1106 20-21 0.09347931061365244 1106 22-23 -0.11775648498846891 1106 24-25 0.004946058083042715 1106 26-27 -0.018407093620773196 1106 28-29 0.3355655514534064 1106 30-31 0.2764633921169022 1106 32-33 0.2651080719755683 1106 34-35 0.06998087480169346 1106 36-37 0.2102719933104069 1106 38-39 0.22625485506915766 1106 40-41 0.1821453413545555 1106 42-43 0.06003697268484132 1106 44-45 0.025734116210820446 1106 46-47 0.3368432925692062 1106 48-49 0.48984895280445784 1106 50-51 0.38201533157180023 1106 52-53 0.410823856390671 1106 54-55 0.43062539589436 1106 56-57 0.5810895512896153 1106 58-59 0.36301973386087383 1106 60-61 0.1849946065768009 1106 62-63 0.3283564716079823 1106 64-65 0.4010087506685096 1106 66-67 0.6833257622704778 1106 68-69 0.568352994969203 1106 70-71 0.3771888425698151 1106 72-73 0.6885765412855207 1106 74-75 0.6895721459243624 1106 76-77 0.7705013214994558 1106 78-79 0.6325429952740294 1106 80-81 0.32021373292605304 1106 82-83 0.2541993290588884 1106 84-85 0.329608785439099 1106 86-87 0.2813848623971573 1106 88-89 0.6229826925885114 1106 90-91 0.9192210789085173 1106 92-93 0.9259944841026702 1106 94-95 0.8054489287663316 1106 96-97 0.7319272568732629 1106 98-99 0.8127738094564805 1106 100-101 0.9007605401328966 1107 1 0.1254013066753501 1107 2 0.1931381855078449 1107 3 0.2549469456334066 1107 4 0.19507194973310504 1107 5 0.22314944300028117 1107 6 0.24259668900670306 1107 7 0.21557586997153066 1107 8 0.14569867273031178 1107 9 0.23867167767396325 1107 10-11 0.2339372014329939 1107 12-13 0.280322163787325 1107 14-15 0.48322990583109515 1107 16-17 0.3698755497995805 1107 18-19 0.4766836209996512 1107 20-21 0.49339867516531655 1107 22-23 0.44734660281960004 1107 24-25 0.3489829855307818 1107 26-27 0.3993308685061905 1107 28-29 0.3973920767574768 1107 30-31 0.4254132520726017 1107 32-33 0.6065654552015189 1107 34-35 0.5455832062253521 1107 36-37 0.5094052690144508 1107 38-39 0.4991542760143872 1107 40-41 0.5524153761456745 1107 42-43 0.5588983386527531 1107 44-45 0.3504218460410655 1107 46-47 0.6781579194466048 1107 48-49 0.5495079733400061 1107 50-51 0.46952806966631755 1107 52-53 0.4186896564540561 1107 54-55 0.6186854769910752 1107 56-57 0.6635695141494651 1107 58-59 0.5941605204494138 1107 60-61 0.7003363761661276 1107 62-63 0.7157601511128249 1107 64-65 0.7357035190167025 1107 66-67 0.8999357250192901 1107 68-69 1.0410258849727256 1107 70-71 0.9123759868534549 1107 72-73 0.7979074037345981 1107 74-75 0.9226905937603149 1107 76-77 0.9624531947787958 1107 78-79 1.0210973742315943 1107 80-81 1.0401307954381735 1107 82-83 1.078078782480386 1107 84-85 1.1702285295069679 1107 86-87 1.0161290455831278 1107 88-89 1.1954452598879328 1107 90-91 1.2557807555467022 1107 92-93 1.3352447965223213 1107 94-95 1.536020718659735 1107 96-97 1.5284481020762648 1107 98-99 1.8650133437184309 1107 100-101 1.8962653701449987 1108 1 0.17055405485627162 1108 2 0.19417428257598246 1108 3 0.14914216122891588 1108 4 0.13366238066856084 1108 5 0.1847120520140919 1108 6 0.05764218144138766 1108 7 0.16567006642235071 1108 8 0.19397973125733614 1108 9 0.2272690727150959 1108 10-11 0.30342886005296066 1108 12-13 0.18141408018568939 1108 14-15 0.16500190228898504 1108 16-17 0.21945683028351937 1108 18-19 0.30188297118683494 1108 20-21 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-0.32672905465187085 2316 80-81 -0.15871358083755993 2316 82-83 -0.06325230182664043 2316 84-85 -0.004173794881502602 2316 86-87 0.005344399576451053 2316 88-89 -0.035343729252712563 2316 90-91 -0.014204256582107888 2316 92-93 -0.19532939521587878 2316 94-95 -0.25210283944261747 2316 96-97 0.13504286687475897 2316 98-99 0.13039709461331483 2316 100-101 0.3741864194786366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8208.0 3 1477.0 4 1463.0 5 1521.0 6 1787.0 7 2032.0 8 2479.0 9 3168.0 10 4069.0 11 5070.0 12 6977.0 13 8997.0 14 10971.0 15 13427.0 16 16699.0 17 21566.0 18 19186.0 19 13873.0 20 13359.0 21 14254.0 22 15440.0 23 17306.0 24 19398.0 25 21897.0 26 25546.0 27 30267.0 28 36296.0 29 44269.0 30 54967.0 31 69611.0 32 89699.0 33 118182.0 34 162727.0 35 229474.0 36 323361.0 37 414589.0 38 292167.0 39 33416.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3124326623848 19.051239012119613 14.64101248029002 22.99531584520556 2 15.231057722484836 19.72270022699256 40.951982129015526 24.09425992150707 3 19.43781463356917 24.13039579816863 29.36595385158632 27.065835716675878 4 12.521042350937881 16.29230309859884 37.21789845499918 33.968756095464094 5 13.5772384405 37.3327256582928 34.19651230888628 14.893523592320923 6 32.105547117797265 35.88784925852429 17.42236954425019 14.584234079428251 7 28.5435113855073 32.72165377951916 20.37622680394277 18.35860803103077 8 26.216437915802192 35.107422686888135 19.55006992774441 19.126069469565266 9 26.05934933849706 17.022296767017284 20.543937828285934 36.37441606619972 10-11 24.89469164596068 26.918704583679204 26.958245739971627 21.228358030388485 12-13 25.64185293383346 24.053806058274116 27.920732533967136 22.38360847392529 14-15 22.61785514752261 25.44067858069174 26.359000984584995 25.58246528720065 16-17 21.813224928344265 28.862204844417228 27.000841487261766 22.32372873997674 18-19 21.72517675423454 27.150928428824304 29.65212642012487 21.471768396816287 20-21 22.370688858921543 26.277386252378403 29.94319546302625 21.408729425673805 22-23 22.007914510721495 26.154880730411058 29.913534339859325 21.923670419008126 24-25 21.81756417847579 26.338115793295007 30.017195240571716 21.827124787657485 26-27 21.54344184895075 26.657010638796585 29.874698720614795 21.924848791637867 28-29 21.581431485436823 26.903495384569904 29.910615304455796 21.60445782553748 30-31 21.692783698962547 26.568332581589598 29.868891735738174 21.869991983709678 32-33 21.409033577684873 26.599681131979164 29.88257129665835 22.108713993677608 34-35 21.951354138976757 26.67979538779574 29.68486681245752 21.683983660769982 36-37 21.92315472413344 26.405333983593852 29.661508918452345 22.010002373820363 38-39 21.593835141999538 26.70891249134149 29.753728930962826 21.943523435696143 40-41 21.936460014019637 26.659382858916093 29.655133560590595 21.749023566473674 42-43 21.670457615650886 26.60839766400569 29.533909557578937 22.18723516276449 44-45 21.73587645344709 26.834778861747687 29.417147887267024 22.012196797538195 46-47 21.95453632267942 27.070539361596495 28.885553366939998 22.089370948784087 48-49 21.96828498248191 27.13128289694005 28.774542069975357 22.12589005060268 50-51 22.081614516719807 27.328918567006944 28.597667351652905 21.991799564620347 52-53 22.172712025665124 27.313267113220867 28.47715068882976 22.036870172284242 54-55 21.931201798988692 27.294637741768547 28.624943510267908 22.149216948974846 56-57 22.054172168519116 27.570693657086615 28.375636123429594 21.999498050964682 58-59 22.085218282348794 27.16148324447434 28.56687998828913 22.18641848488774 60-61 21.98040274071656 27.299949069843578 28.745686751371718 21.973961438068145 62-63 21.809512397341067 27.38294209171523 28.792109181473723 22.015436329469978 64-65 21.776202961377237 27.30735948326895 28.735799639270894 22.180637916082922 66-67 21.77207076979269 27.442422949504802 28.510166618066744 22.27533966263576 68-69 21.813522955508933 27.278967495617202 28.71078485359163 22.19672469528223 70-71 21.89768483115559 27.320894888305205 28.549569992574614 22.231850287964594 72-73 21.89953645458989 27.364140466934785 28.43918162774466 22.297141450730667 74-75 21.7682274360245 27.688550565943803 28.296705199829493 22.246516798202208 76-77 21.96232712794512 27.44115071249943 28.289564977865496 22.306957181689953 78-79 21.962820966337155 27.493380018786244 28.277156238945533 22.26664277593107 80-81 21.835259858689405 27.763946057136103 28.19527562400327 22.205518460171227 82-83 22.022656075055462 27.64625219023346 28.183197013664284 22.147894721046786 84-85 21.984433102880217 27.65795210995264 28.07309552984979 22.284519257317356 86-87 21.981064494808102 27.586155948390118 28.189597915436863 22.24318164136492 88-89 22.014901941952886 27.592302556425974 28.02981681240471 22.36297868921643 90-91 22.062896932730357 27.612399481804157 28.04119707765619 22.283506507809303 92-93 22.057105204649186 27.436388608695832 28.16789450098457 22.33861168567041 94-95 21.901645369610208 27.457849928476243 28.175257674836107 22.465247027077446 96-97 22.004703068307624 27.39286782115411 28.093133220124145 22.509295890414123 98-99 21.94473518509924 27.39518154800869 27.974692517224508 22.685390749667565 100-101 22.31411751125476 26.874754703913194 28.319496710146602 22.491631074685444 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2655.0 1 1601.0 2 671.0 3 763.0 4 600.0 5 513.0 6 912.0 7 1915.5 8 3208.0 9 3836.5 10 3291.5 11 2637.0 12 2658.5 13 2933.5 14 3101.5 15 3198.5 16 3279.5 17 3431.0 18 3692.5 19 3950.0 20 4331.0 21 5078.5 22 6019.5 23 7213.0 24 8893.5 25 11149.5 26 13942.0 27 16992.0 28 19855.0 29 23025.5 30 26873.5 31 30869.0 32 35556.5 33 39761.0 34 43602.0 35 47443.0 36 52353.5 37 57463.5 38 60624.0 39 63111.0 40 65279.5 41 68951.0 42 71691.0 43 74196.5 44 77909.0 45 80554.0 46 81949.5 47 83049.5 48 84543.5 49 83371.0 50 79622.0 51 75435.5 52 72178.0 53 70217.0 54 68375.5 55 63243.0 56 56198.5 57 50599.0 58 46072.5 59 40965.5 60 34073.0 61 27767.0 62 22535.5 63 17106.5 64 12757.0 65 9941.5 66 7308.0 67 5470.5 68 4114.0 69 2901.0 70 2200.5 71 1607.0 72 1231.5 73 1052.5 74 862.5 75 666.0 76 489.0 77 316.5 78 198.0 79 147.5 80 103.5 81 70.0 82 49.5 83 33.0 84 19.5 85 17.5 86 16.5 87 12.0 88 10.0 89 7.5 90 7.5 91 7.5 92 5.5 93 4.0 94 3.0 95 3.0 96 2.0 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3037067155695331 2 0.2623550270653025 3 0.2567769286160138 4 0.26295432689869713 5 0.2655820261681967 6 0.1994285445588646 7 0.18997804718610287 8 0.18928654737833983 9 0.20076544418720652 10-11 0.20122644405904858 12-13 0.19705439521887813 14-15 0.1994515945524567 16-17 0.183662348941867 18-19 0.18972449725658977 20-21 0.17877575030034143 22-23 0.18721204795505067 24-25 0.18735034791660327 26-27 0.18700459801272176 28-29 0.19758454507149648 30-31 0.19507209576995738 32-33 0.20065019421924601 34-35 0.19474939585966794 36-37 0.18034314986460434 38-39 0.17047775260718478 40-41 0.16891035304292185 42-43 0.1822332493391567 44-45 0.1824406992814856 46-47 0.1822332493391567 48-49 0.19790724498178588 50-51 0.19320504628899715 52-53 0.20968579170734988 54-55 0.18354709897390648 56-57 0.16778090335690887 58-59 0.17003980272893485 60-61 0.16075065531131782 62-63 0.1656142039592513 64-65 0.16522235406818556 66-67 0.16395460442061996 68-69 0.16727380349788262 70-71 0.1684493531710798 72-73 0.16625960377983015 74-75 0.1787296503131572 76-77 0.16999370274175063 78-79 0.17444235150502627 80-81 0.16985540278019803 82-83 0.15365125728495047 84-85 0.15305195745155584 86-87 0.1554722067787265 88-89 0.15858395591366026 90-91 0.16630570376701437 92-93 0.16266380477946227 94-95 0.1606123553497652 96-97 0.1655220039848829 98-99 0.17294410192153967 100-101 0.15895275581113388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2169198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.93558650672567 #Duplication Level Percentage of deduplicated Percentage of total 1 78.52346673461106 45.49303099811055 2 12.439157798504281 14.41339805412112 3 3.788954826901957 6.585459604321624 4 1.7200439884060048 3.986070291426781 5 0.8874602450216318 2.5707764898365357 6 0.5503749949741797 1.913177887947918 7 0.38024293838986495 1.5420718355460306 8 0.2564410268219966 1.1885649034655472 9 0.20541374454520886 1.0710689190092457 >10 1.1254227402655903 12.049813289431516 >50 0.08385979075785475 3.3258080272257335 >100 0.03676536607930625 3.7461521393627453 >500 0.001916643776802579 0.7394001273295855 >1k 2.3958047210030452E-4 0.2940759157315921 >5k 1.5972031473353635E-4 0.6095273936198858 >10k+ 7.986015736676817E-5 0.4716041235135491 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10193 0.4698971693685869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7905 0.3644203986911292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5269 0.24290083247356856 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2789 0.12857286425674372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.6099987184203564E-5 0.0 0.0 0.0 4 0.0 4.6099987184203564E-5 0.0 0.0 0.0 5 0.0 4.6099987184203564E-5 0.0 0.0 0.0 6 0.0 4.6099987184203564E-5 0.0 0.0 0.0 7 0.0 4.6099987184203564E-5 0.0 0.0 0.0 8 0.0 4.6099987184203564E-5 0.0 0.0 0.0 9 0.0 4.6099987184203564E-5 0.0 0.0 0.0 10-11 0.0 4.6099987184203564E-5 0.0 4.6099987184203564E-5 0.0 12-13 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 4.6099987184203564E-5 14-15 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 4.6099987184203564E-5 16-17 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 4.6099987184203564E-5 18-19 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 4.6099987184203564E-5 20-21 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 4.6099987184203564E-5 22-23 0.0 4.6099987184203564E-5 0.0 1.3829996155261069E-4 9.219997436840713E-5 24-25 0.0 4.6099987184203564E-5 0.0 1.3829996155261069E-4 9.219997436840713E-5 26-27 0.0 4.6099987184203564E-5 0.0 2.0744994232891603E-4 9.219997436840713E-5 28-29 0.0 4.6099987184203564E-5 0.0 8.067497757235623E-4 9.219997436840713E-5 30-31 0.0 4.6099987184203564E-5 0.0 0.002512449301539094 1.1524996796050891E-4 32-33 0.0 4.6099987184203564E-5 0.0 0.005393698500551817 1.3829996155261069E-4 34-35 0.0 4.6099987184203564E-5 0.0 0.009427447379169628 1.8439994873681426E-4 36-37 0.0 6.914998077630534E-5 0.0 0.015443495706708193 1.8439994873681426E-4 38-39 0.0 9.219997436840713E-5 0.0 0.026276992694996032 1.8439994873681426E-4 40-41 0.0 9.219997436840713E-5 0.0 0.03738708960638909 1.8439994873681426E-4 42-43 0.0 9.219997436840713E-5 0.0 0.04856633649855845 2.3049993592101783E-4 44-45 0.0 9.219997436840713E-5 0.0 0.06235023266663532 2.3049993592101783E-4 46-47 0.0 9.219997436840713E-5 0.0 0.08005262774536949 2.3049993592101783E-4 48-49 0.0 9.219997436840713E-5 0.0 0.09980647225380071 3.226999102894249E-4 50-51 0.0 9.219997436840713E-5 0.0 0.12336356570492873 3.226999102894249E-4 52-53 0.0 9.219997436840713E-5 0.0 0.14791180888051714 3.226999102894249E-4 54-55 0.0 1.1524996796050891E-4 0.0 0.1784069504028678 3.226999102894249E-4 56-57 0.0 1.8439994873681426E-4 0.0 0.21176029113063904 3.226999102894249E-4 58-59 0.0 1.8439994873681426E-4 0.0 0.25412617935292214 3.226999102894249E-4 60-61 0.0 1.8439994873681426E-4 0.0 0.30006481658198103 3.226999102894249E-4 62-63 0.0 1.8439994873681426E-4 0.0 0.3498067027537366 3.226999102894249E-4 64-65 0.0 1.8439994873681426E-4 0.0 0.4024528881180971 3.226999102894249E-4 66-67 0.0 1.8439994873681426E-4 0.0 0.4665779702913243 3.457499038815267E-4 68-69 0.0 1.8439994873681426E-4 0.0 0.5402226998180895 3.687998974736285E-4 70-71 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.6224881269483007 3.687998974736285E-4 72-73 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.7102625025470244 3.687998974736285E-4 74-75 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.8063809758260887 3.687998974736285E-4 76-77 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.9145776457474144 3.687998974736285E-4 78-79 4.6099987184203564E-5 1.8439994873681426E-4 0.0 1.0357514620610937 3.687998974736285E-4 80-81 4.6099987184203564E-5 2.0744994232891603E-4 0.0 1.1755035732100065 3.687998974736285E-4 82-83 9.219997436840713E-5 2.3049993592101783E-4 0.0 1.318713183397735 3.687998974736285E-4 84-85 9.219997436840713E-5 2.3049993592101783E-4 0.0 1.4692757415413438 3.687998974736285E-4 86-87 9.219997436840713E-5 2.535499295131196E-4 0.0 1.6329998460260429 3.687998974736285E-4 88-89 9.219997436840713E-5 2.7659992310522137E-4 0.0 1.8166391449743178 3.687998974736285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5210 0.0 45.635666 1 GTATCAA 8815 0.0 39.217888 1 ATCAACG 11060 0.0 30.858213 3 TATCAAC 11170 0.0 30.852213 2 TCAACGC 11155 0.0 30.596828 4 CAACGCA 11325 0.0 30.011614 5 AACGCAG 11760 0.0 28.882803 6 GTACATG 8575 0.0 26.008234 1 TACATGG 8495 0.0 25.459476 2 ACGCAGA 13480 0.0 24.986588 7 CGCAGAG 13540 0.0 24.805695 8 ACATGGG 8740 0.0 23.984388 3 GCAGAGT 14470 0.0 22.52144 9 GAGTACT 8360 0.0 22.329899 12-13 GTACTTT 9010 0.0 20.58646 14-15 AGTACTT 8905 0.0 20.376514 12-13 AGAGTAC 12770 0.0 20.29104 10-11 CATGGGG 4085 0.0 19.433273 4 GTACCTG 1640 0.0 19.136929 1 GAGTACA 6565 0.0 19.122353 1 >>END_MODULE