FastQCFastQC Report
Thu 26 May 2016
SRR937526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169198
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107280.4945606625121358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.4299284804798824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64750.29849741701771804No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24150.1113314690498516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.045.1785281
GTATCAA103850.037.6106761
ATCAACG133650.029.0052683
TATCAAC135550.028.6687972
TCAACGC135300.028.6515464
CAACGCA138450.028.0339815
AACGCAG143300.027.0801756
ACGCAGA165300.023.3903757
CGCAGAG165850.023.2555278
GCAGAGT175100.021.7828669
GAGTACT108300.021.49083312-13
AGTACTT112000.020.54761312-13
AGAGTAC157250.020.3740310-11
TGCACCG3503.6379788E-1220.3600545
GTACTTT113950.020.17467914-15
GTACCGT2151.0801286E-619.8828966
GTACATG87100.019.14851
TACATGG85950.019.013772
CAGAGTA172750.018.9996510-11
GCACCGT3506.366463E-1118.9992126
Produced by FastQC (version 0.11.5)