##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937526_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2169198 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.634527138601456 31.0 31.0 34.0 30.0 34.0 2 31.873126381270865 31.0 31.0 34.0 30.0 34.0 3 31.962977100292367 31.0 31.0 34.0 30.0 34.0 4 35.73014588801944 37.0 35.0 37.0 33.0 37.0 5 35.60866458479124 37.0 35.0 37.0 33.0 37.0 6 35.65864895689559 37.0 35.0 37.0 33.0 37.0 7 35.5846363494711 37.0 35.0 37.0 33.0 37.0 8 35.55433113989594 37.0 35.0 37.0 33.0 37.0 9 37.12886052817677 39.0 37.0 39.0 33.0 39.0 10-11 37.02420733377036 39.0 37.0 39.0 33.0 39.0 12-13 36.8952852621107 39.0 37.0 39.0 33.0 39.0 14-15 37.917554782919765 40.0 37.0 41.0 33.0 41.0 16-17 37.84933717438427 39.0 37.0 41.0 33.0 41.0 18-19 37.81438301160152 39.0 37.0 41.0 33.0 41.0 20-21 37.67653828742235 39.0 37.0 40.5 32.0 41.0 22-23 37.47872208991526 39.0 36.0 40.0 32.0 41.0 24-25 37.56879524137493 39.0 36.5 41.0 32.0 41.0 26-27 37.39278364630614 39.0 36.0 40.5 32.0 41.0 28-29 37.20700945695137 39.0 36.0 40.0 31.0 41.0 30-31 37.22793147513505 39.0 36.0 40.0 32.0 41.0 32-33 37.12529008416936 39.0 36.0 40.0 31.0 41.0 34-35 36.950179974349965 39.0 35.5 40.0 31.0 41.0 36-37 36.72566957926386 39.0 35.0 40.0 30.5 41.0 38-39 36.5331866892741 39.0 35.0 40.0 30.0 41.0 40-41 36.30012636006487 38.0 35.0 40.0 30.0 41.0 42-43 36.027955032228505 38.0 35.0 40.0 30.0 41.0 44-45 35.650941961038136 38.0 35.0 40.0 28.5 41.0 46-47 35.25254402779276 38.0 34.0 40.0 27.0 41.0 48-49 34.86406035779122 38.0 34.0 40.0 26.0 41.0 50-51 34.5473737298301 38.0 33.5 40.0 24.5 41.0 52-53 34.221711434364224 38.0 33.0 40.0 24.0 41.0 54-55 33.92714772925293 37.5 33.0 40.0 23.5 41.0 56-57 33.576712683673875 37.0 32.5 40.0 23.0 41.0 58-59 33.23404617743516 37.0 32.0 39.0 22.0 40.0 60-61 32.78023144959566 36.0 31.0 39.0 20.5 40.0 62-63 32.332699228009616 36.0 31.0 39.0 19.0 40.0 64-65 31.839429826138506 35.0 30.5 38.5 18.0 40.0 66-67 31.2899057624062 35.0 29.5 38.0 14.0 40.0 68-69 30.756825333602556 34.0 29.0 37.5 10.5 39.5 70-71 30.256409281218218 34.0 29.0 37.0 8.0 39.0 72-73 29.644242480400592 34.0 28.0 36.0 7.0 39.0 74-75 29.019152239675677 33.0 27.0 36.0 6.5 38.5 76-77 28.015276383253166 31.5 26.0 34.5 2.0 36.5 78-79 28.62247683245144 33.0 27.0 35.0 2.0 37.0 80-81 28.352599900977225 33.0 26.5 35.0 2.0 37.0 82-83 27.872740293878195 33.0 26.0 35.0 2.0 36.0 84-85 27.322713279285708 32.0 26.0 35.0 2.0 36.0 86-87 26.713703866590325 32.0 25.0 34.0 2.0 35.5 88-89 26.099302368893945 31.5 24.5 34.0 2.0 35.0 90-91 25.27171309396376 31.0 21.0 34.0 2.0 35.0 92-93 24.481516671138365 31.0 18.5 34.0 2.0 35.0 94-95 23.680933690700435 30.5 10.5 34.0 2.0 35.0 96-97 22.742496074586093 30.0 2.0 34.0 2.0 35.0 98-99 21.41802707728847 29.0 2.0 34.0 2.0 35.0 100-101 18.7014539474958 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17290372226045747 1101 2 0.24870730040980504 1101 3 0.26172395618445066 1101 4 0.07601965490395202 1101 5 0.13837016158296933 1101 6 0.1336170350459227 1101 7 0.1852544512243881 1101 8 0.10450937960657569 1101 9 0.22975713368950323 1101 10-11 0.20123036104843806 1101 12-13 0.24920732824822522 1101 14-15 0.36772150530983794 1101 16-17 0.35720651731872266 1101 18-19 0.2942504089845954 1101 20-21 0.3169972985141314 1101 22-23 0.3116188678871694 1101 24-25 0.3980220522214708 1101 26-27 0.2230657800259408 1101 28-29 0.3697094941120582 1101 30-31 0.4574125732717249 1101 32-33 0.46499844979965843 1101 34-35 0.4539289628684742 1101 36-37 0.42037474404996544 1101 38-39 0.26682379911846965 1101 40-41 0.2744227463031166 1101 42-43 0.4704886260472847 1101 44-45 0.4132968735209701 1101 46-47 0.491289586738354 1101 48-49 0.49403869330374306 1101 50-51 0.3419808127083712 1101 52-53 0.3927367626950655 1101 54-55 0.49150474004414235 1101 56-57 0.5893187074822279 1101 58-59 0.548041098474414 1101 60-61 0.6707437347846934 1101 62-63 0.690544443436238 1101 64-65 0.750259191579179 1101 66-67 0.7908841120017698 1101 68-69 0.727041135699892 1101 70-71 0.704257581370328 1101 72-73 0.825392594789264 1101 74-75 0.8486334428170075 1101 76-77 0.6771397672803943 1101 78-79 0.6069123171275308 1101 80-81 0.5979801497025115 1101 82-83 0.7223938210516359 1101 84-85 0.7092875623432384 1101 86-87 0.5341739798943834 1101 88-89 0.7427032617815179 1101 90-91 0.9315381471960258 1101 92-93 0.9900453093292079 1101 94-95 1.1043550988127109 1101 96-97 1.3112135344930707 1101 98-99 1.3305849856864107 1101 100-101 1.7505499768347086 1102 1 0.1964485683536168 1102 2 0.24802949077743364 1102 3 0.17065982840803073 1102 4 0.14233039253528545 1102 5 0.16600959564176065 1102 6 0.20830228443757193 1102 7 0.18652652896012967 1102 8 0.20370932906043748 1102 9 0.3720617039903118 1102 10-11 0.33933091451106634 1102 12-13 0.3267746726367733 1102 14-15 0.3813597476732866 1102 16-17 0.3738630484322343 1102 18-19 0.36422073945627886 1102 20-21 0.3949552346522509 1102 22-23 0.38294104421419917 1102 24-25 0.2924832137470901 1102 26-27 0.3238614428867308 1102 28-29 0.33086005051444545 1102 30-31 0.41681709416720025 1102 32-33 0.45504226522552926 1102 34-35 0.35729387876093455 1102 36-37 0.3549640961906704 1102 38-39 0.47379978005134404 1102 40-41 0.43991860301184715 1102 42-43 0.4765096711412866 1102 44-45 0.44099159241512353 1102 46-47 0.4457258437619416 1102 48-49 0.4746511790766661 1102 50-51 0.5182592306494342 1102 52-53 0.33019267742653824 1102 54-55 0.34918068842039673 1102 56-57 0.052448373237133694 1102 58-59 0.06690568836185662 1102 60-61 0.19176610057198218 1102 62-63 0.18778783624026119 1102 64-65 0.4352368099962156 1102 66-67 0.29112991013489875 1102 68-69 0.3468326679936986 1102 70-71 0.2655395928256965 1102 72-73 0.5262863662944071 1102 74-75 0.42484341286666805 1102 76-77 0.4700110948974512 1102 78-79 0.5914968866912105 1102 80-81 0.5535954655324922 1102 82-83 0.5323267508934215 1102 84-85 0.46582569615535263 1102 86-87 0.7307177123930018 1102 88-89 0.7216059543285098 1102 90-91 1.0755997222171096 1102 92-93 1.1853071356769043 1102 94-95 1.3299339003252335 1102 96-97 1.3288432542165047 1102 98-99 1.3206813655827396 1102 100-101 1.4229480136087744 1103 1 0.2688407342297694 1103 2 0.2869875063370273 1103 3 0.2393579834050037 1103 4 0.07104965119091844 1103 5 0.0753011256221896 1103 6 0.1515404075352862 1103 7 0.12320667271574592 1103 8 0.18120729182606254 1103 9 0.19751755694726114 1103 10-11 0.14116738553358488 1103 12-13 0.19174634289036163 1103 14-15 0.27786301769564403 1103 16-17 0.24427143556945197 1103 18-19 0.1896817290298216 1103 20-21 0.08520079974972106 1103 22-23 0.21102040232866415 1103 24-25 0.10441661881893083 1103 26-27 0.14358535409812845 1103 28-29 0.06857396012392769 1103 30-31 0.29598027838193985 1103 32-33 0.38663097720586137 1103 34-35 0.34564603474692035 1103 36-37 0.30104163646522863 1103 38-39 0.27516925427075023 1103 40-41 0.08942017708011463 1103 42-43 0.5156149073559959 1103 44-45 0.3166413308829874 1103 46-47 0.46639558891610733 1103 48-49 0.6358623236193139 1103 50-51 0.5115139824550852 1103 52-53 0.3194067402639007 1103 54-55 0.4255178628981966 1103 56-57 0.3992521788605927 1103 58-59 0.317886520946459 1103 60-61 0.2648956756431957 1103 62-63 0.26253527141442135 1103 64-65 0.4897462369935788 1103 66-67 0.5317854468852126 1103 68-69 0.37048452379232444 1103 70-71 0.5352901447943275 1103 72-73 0.4093833282033472 1103 74-75 0.2471015914050163 1103 76-77 0.20522695468000762 1103 78-79 0.34632783106252063 1103 80-81 0.40287964753936123 1103 82-83 0.6510056653435079 1103 84-85 0.7125033499578457 1103 86-87 0.9905299559974807 1103 88-89 1.1305276479145299 1103 90-91 1.0551728780846936 1103 92-93 1.0766016152941127 1103 94-95 1.1958103115888754 1103 96-97 1.4755728836139745 1103 98-99 1.5420968778165935 1103 100-101 1.8940594207283006 1104 1 0.28063837895297894 1104 2 0.2779037486012399 1104 3 0.16823595067873143 1104 4 0.06163722262367344 1104 5 0.127619039193263 1104 6 0.20314556198617595 1104 7 0.20604792923514026 1104 8 0.20559532974136374 1104 9 0.2193058538956052 1104 10-11 0.2710183300004161 1104 12-13 0.350863717064847 1104 14-15 0.34948433034351467 1104 16-17 0.24561975276085235 1104 18-19 0.27098933355969024 1104 20-21 0.29848891267028677 1104 22-23 0.2718182324738052 1104 24-25 0.196672830802207 1104 26-27 0.22436518576848385 1104 28-29 0.19472674779629529 1104 30-31 0.3535601492278957 1104 32-33 0.3665434559624998 1104 34-35 0.3581622765928856 1104 36-37 0.43999010589779175 1104 38-39 0.4259537075479116 1104 40-41 0.42268603936877724 1104 42-43 0.3021253397794652 1104 44-45 0.4850878331700912 1104 46-47 0.5148924862355742 1104 48-49 0.46461828535269234 1104 50-51 0.4811482655762589 1104 52-53 0.4796014437491394 1104 54-55 0.3208524972057134 1104 56-57 0.5288262103098305 1104 58-59 0.35248426688495726 1104 60-61 0.4395047560201206 1104 62-63 0.6525202041397193 1104 64-65 0.5592216044566278 1104 66-67 0.48816877117980795 1104 68-69 0.5942481045547297 1104 70-71 0.6859975712912565 1104 72-73 0.5708594296863012 1104 74-75 0.5806482006234113 1104 76-77 0.5184100881154841 1104 78-79 0.7061994751695195 1104 80-81 0.7729544645920434 1104 82-83 0.7323749610050534 1104 84-85 0.7859829772458191 1104 86-87 0.9123255548057614 1104 88-89 1.0613244882132697 1104 90-91 1.0659822448199954 1104 92-93 1.1996756843853937 1104 94-95 1.369360622275174 1104 96-97 1.5328124947576889 1104 98-99 1.600756953407398 1104 100-101 1.701453869641771 1105 1 0.11257814720971737 1105 2 0.10341019311055177 1105 3 0.1580222666911517 1105 4 0.06511250559141502 1105 5 0.0424934595735067 1105 6 -0.037832648171828964 1105 7 0.0222209215795246 1105 8 0.09669428988070194 1105 9 0.13388151143545457 1105 10-11 0.10329384562791688 1105 12-13 0.18357763468640798 1105 14-15 0.15197710573123402 1105 16-17 0.20562946255316916 1105 18-19 0.18804791597331416 1105 20-21 0.16712301803614338 1105 22-23 0.209245007613994 1105 24-25 0.2704169173366253 1105 26-27 0.08474380272024007 1105 28-29 0.06335489955482387 1105 30-31 0.15376105641332316 1105 32-33 0.3007716104633005 1105 34-35 0.13622640885100878 1105 36-37 -0.017369978015594256 1105 38-39 0.02667719712675165 1105 40-41 0.03915618888370176 1105 42-43 -0.022289519847106476 1105 44-45 0.06218659702079776 1105 46-47 0.017979161015986733 1105 48-49 0.13155256366182044 1105 50-51 0.16998099410727718 1105 52-53 0.32426497292487966 1105 54-55 0.2638714235430015 1105 56-57 0.20448937902170883 1105 58-59 0.33076884498527903 1105 60-61 0.27147627684926334 1105 62-63 0.392103556266008 1105 64-65 0.4633731270105166 1105 66-67 0.5732822631725654 1105 68-69 0.2144956375061433 1105 70-71 0.3165134531702485 1105 72-73 0.32096370802069174 1105 74-75 0.3720028591768134 1105 76-77 0.34087378824996506 1105 78-79 0.5811443140955745 1105 80-81 0.3309422391300174 1105 82-83 0.5841707313459175 1105 84-85 0.6074106594992692 1105 86-87 0.6876218008939716 1105 88-89 0.6546254531667266 1105 90-91 0.7730011689923266 1105 92-93 0.79282537358905 1105 94-95 0.9370445679941533 1105 96-97 1.0942051012433716 1105 98-99 1.2344361853126706 1105 100-101 1.0501165090238338 1106 1 0.05336699689851088 1106 2 0.06695617617175742 1106 3 0.14776981737615458 1106 4 0.11780834583745303 1106 5 0.1456993761794081 1106 6 0.219173613305081 1106 7 0.11122656421665056 1106 8 0.11974024106699233 1106 9 0.12342381877492414 1106 10-11 0.03373015099890608 1106 12-13 -0.0437214017582761 1106 14-15 0.18596470405756804 1106 16-17 0.279502101282894 1106 18-19 0.22701771876866417 1106 20-21 0.19872796657760006 1106 22-23 0.22903524735999525 1106 24-25 0.30521091210292184 1106 26-27 0.30086698915427235 1106 28-29 0.3591875267162976 1106 30-31 0.37810894232912773 1106 32-33 0.38102992165263316 1106 34-35 0.3216937028574378 1106 36-37 0.181419280605013 1106 38-39 0.23555972485404197 1106 40-41 0.3615326502707461 1106 42-43 0.28482566054158553 1106 44-45 0.4351308594586243 1106 46-47 0.43783046843164897 1106 48-49 0.5289796750638729 1106 50-51 0.6805943685100146 1106 52-53 0.3921521248469375 1106 54-55 0.4098606653709709 1106 56-57 0.3228034215537292 1106 58-59 0.439495924201438 1106 60-61 0.5204257652180289 1106 62-63 0.5828152453409956 1106 64-65 0.6418026733449871 1106 66-67 0.8613251668061537 1106 68-69 0.6501193906916889 1106 70-71 0.7609251588823867 1106 72-73 0.8263873022981372 1106 74-75 0.5429324715934172 1106 76-77 0.5483159197332057 1106 78-79 0.7531547581830864 1106 80-81 0.6927730408696107 1106 82-83 0.5408428115649642 1106 84-85 0.7260473383509627 1106 86-87 0.8969626276825764 1106 88-89 0.7500921796442057 1106 90-91 1.0474822687234884 1106 92-93 1.2401361415567855 1106 94-95 1.517777698779252 1106 96-97 1.5881459522880128 1106 98-99 1.5699313115606515 1106 100-101 1.6094411651246183 1107 1 0.25013713718198005 1107 2 0.34038081662222197 1107 3 0.3081196846778802 1107 4 0.24289515280182883 1107 5 0.16461646090044013 1107 6 0.19854558787416465 1107 7 0.19295665584551358 1107 8 0.2146878408384083 1107 9 0.3341289860208718 1107 10-11 0.3120701156867369 1107 12-13 0.2845743159356502 1107 14-15 0.35324353955868304 1107 16-17 0.36540501329886155 1107 18-19 0.36760335933785626 1107 20-21 0.405905226185304 1107 22-23 0.3902823335075212 1107 24-25 0.4361473126014275 1107 26-27 0.4247854790163288 1107 28-29 0.45256128312377797 1107 30-31 0.408629011074261 1107 32-33 0.47695215115444967 1107 34-35 0.47174760218783973 1107 36-37 0.3654390473943181 1107 38-39 0.6177281863143165 1107 40-41 0.6644170972815502 1107 42-43 0.6317985426011319 1107 44-45 0.6197349983463525 1107 46-47 0.6492830511150132 1107 48-49 0.6719947361255763 1107 50-51 0.7306599915854193 1107 52-53 0.8290146328656363 1107 54-55 0.911148248622375 1107 56-57 0.8726768520880839 1107 58-59 0.8711205448347101 1107 60-61 0.879722139783361 1107 62-63 0.9562191930612016 1107 64-65 1.0472219477797005 1107 66-67 0.990866890098431 1107 68-69 0.9326579082123878 1107 70-71 1.0494236738746885 1107 72-73 1.117528571503506 1107 74-75 1.0624893439288812 1107 76-77 0.9316848371596009 1107 78-79 1.0842035272539263 1107 80-81 0.8843249401163007 1107 82-83 0.9972745776606651 1107 84-85 1.2212641998723974 1107 86-87 1.5025077241675042 1107 88-89 1.4493346634210234 1107 90-91 1.5217158689339634 1107 92-93 1.7888469139914207 1107 94-95 2.008875660598708 1107 96-97 2.0096962670139753 1107 98-99 1.8234196061807104 1107 100-101 1.6631685899751965 1108 1 0.13586723740973028 1108 2 0.10776563095320313 1108 3 0.050594723576775635 1108 4 0.009162089741110435 1108 5 -0.05512300913750323 1108 6 -0.08840069650924676 1108 7 -0.0060442612015521036 1108 8 -0.03173820994291532 1108 9 -0.030975009160904676 1108 10-11 0.050276711525391704 1108 12-13 0.10619311484678207 1108 14-15 -0.05544659948064634 1108 16-17 -0.07966043864603733 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-0.03271023641585913 2316 66-67 -0.41058709576709873 2316 68-69 -0.06788663250373261 2316 70-71 0.09436952930210651 2316 72-73 0.049597339144320074 2316 74-75 0.07667188341410736 2316 76-77 0.030054284632797135 2316 78-79 0.10757324109923871 2316 80-81 0.19432982614201322 2316 82-83 0.23637742114497584 2316 84-85 0.41840379459102195 2316 86-87 0.2300432949928073 2316 88-89 0.4630845384798121 2316 90-91 0.6035673276637858 2316 92-93 0.5461158285681655 2316 94-95 0.39730189353376844 2316 96-97 0.5135103352163988 2316 98-99 0.8152371620820738 2316 100-101 1.1291389176433455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 26.0 5 68.0 6 167.0 7 390.0 8 974.0 9 1657.0 10 2831.0 11 4408.0 12 6486.0 13 9363.0 14 12042.0 15 16419.0 16 21097.0 17 26960.0 18 24672.0 19 19080.0 20 18193.0 21 19185.0 22 20897.0 23 23494.0 24 27624.0 25 32748.0 26 39454.0 27 48646.0 28 58524.0 29 71478.0 30 85692.0 31 104025.0 32 127222.0 33 157003.0 34 198326.0 35 252779.0 36 310830.0 37 302619.0 38 119763.0 39 4035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.191274200739855 21.0104542308673 15.96841921313619 21.829852355256648 2 16.50504011159885 21.571382603155637 38.72177643534615 23.201800849899364 3 20.1931312863095 24.760303116635736 30.260769187506163 24.7857964095486 4 13.864827212319414 17.835288130061212 36.45289448523276 31.846990172386608 5 13.475397393729772 37.79691270272003 34.24334380200425 14.484346101545952 6 31.868275740619346 37.085457390242844 16.642510273382143 14.403756595755665 7 28.255834644877968 33.135887088223384 20.305707455013327 18.302570811885314 8 26.319865683077342 35.97845839798857 19.080922995503407 18.620752923430686 9 25.902338099150008 17.00370367297038 20.354757841377317 36.73920038650229 10-11 25.07657207871296 27.408355530477163 26.603611104196112 20.91146128661376 12-13 25.730700292095715 24.442662386038418 27.855954769421416 21.97068255244446 14-15 22.510531542072233 25.89793555037392 26.304767937274516 25.28676497027934 16-17 21.666993976575675 29.256457916704697 27.049951180113574 22.026596926606054 18-19 21.540818311652508 27.407617930682214 29.926728680369425 21.12483507729585 20-21 22.139242245290657 26.376960517204978 30.43747043838322 21.04632679912115 22-23 22.013433647737855 26.23316183995796 30.30914907937562 21.44425543292857 24-25 21.82092183378373 26.526877675528006 30.369519057273703 21.28268143341456 26-27 21.505287117872477 26.847702192641254 30.265779237617775 21.381231451868498 28-29 21.465456774204554 26.97246209212864 30.3173996916337 21.244681442033105 30-31 21.606879593287474 26.668565986138653 30.341144515161826 21.383409905412044 32-33 21.48973952585241 26.652684540553693 30.27114168462261 21.58643424897128 34-35 21.98549878803134 26.701596626956135 30.129522523992737 21.18338206101979 36-37 21.974734441023823 26.494331084575958 30.032989150829014 21.497945323571198 38-39 21.619515973072993 26.795047477235244 30.164311917195185 21.42112463249658 40-41 21.950850147463388 26.654465449541476 29.98362286253001 21.41106154046513 42-43 21.742257631713628 26.69027299426901 29.82675629694178 21.740713077075583 44-45 21.88392251051368 26.92620197100541 29.636028992288622 21.55384652619229 46-47 22.13961104518813 27.222457332156864 29.03013002962385 21.607801593031155 48-49 22.18384398289137 27.24154272685112 28.90851826343192 21.666095026825584 50-51 22.240109017249694 27.49866540537102 28.659347832701304 21.601877744677985 52-53 22.39143222518184 27.55078144088276 28.447887191487357 21.60989914244804 54-55 22.136568446033973 27.521185249110502 28.56382865925563 21.778417645599895 56-57 22.1931100803154 27.750394385390358 28.41050010188097 21.64599543241327 58-59 22.338601639868745 27.371429440742617 28.524920270072162 21.765048649316476 60-61 22.169276386941164 27.504750603679334 28.76602320304555 21.559949806333954 62-63 22.0369694237225 27.542713943125523 28.79866199397196 21.62165463918001 64-65 22.03662367381862 27.438412722121264 28.693623173172757 21.831340430887362 66-67 21.9677963929526 27.580562032603755 28.496407427998733 21.95523414644491 68-69 22.028358881189675 27.47947742874437 28.618562537196944 21.87360115286901 70-71 22.143552594092377 27.48833900824176 28.446942141750085 21.92116625591578 72-73 22.140141195040748 27.562329487672404 28.35103111841335 21.946498198873503 74-75 22.057115118122 27.879750949429237 28.065372547826435 21.997761384622336 76-77 22.135692546277472 27.619608721748772 28.30398608149187 21.940712650481885 78-79 22.165473137998468 27.640468965949626 28.233868000984696 21.96018989506721 80-81 22.073703327476327 27.895305934709004 28.120504527828622 21.910486209986047 82-83 22.227126849252485 27.808036637333412 28.08415817506691 21.880678338347195 84-85 22.146167137196038 27.84972994503012 27.981438215590348 22.02266470218349 86-87 22.22153072241446 27.77349047896965 28.007194363999965 21.997784434615927 88-89 22.175108035319965 27.760328932628557 28.02215380984124 22.042409222210235 90-91 22.212952574417354 27.750059763486433 27.986116890233205 22.050870771863003 92-93 22.249557545786345 27.612346950488924 28.065957602767167 22.072137900957564 94-95 22.12315334976337 27.6064010754205 28.00244606531999 22.267999509496136 96-97 22.134424796629908 27.57438463432107 27.9469877807374 22.344202788311627 98-99 22.04409362715562 27.589358446622352 27.809766651284345 22.556781274937684 100-101 22.320423443584545 27.28422929011783 28.101182710447464 22.294164555850166 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 703.0 1 505.5 2 432.5 3 608.5 4 508.5 5 425.0 6 828.0 7 1815.0 8 2916.5 9 3475.5 10 3212.0 11 2733.0 12 2734.5 13 2972.0 14 3303.5 15 3610.5 16 3798.0 17 3944.0 18 4149.0 19 4506.5 20 4977.0 21 5642.0 22 6595.0 23 7932.0 24 9582.0 25 11618.5 26 14464.5 27 17719.5 28 20550.0 29 23899.0 30 27952.0 31 31706.5 32 36534.5 33 40731.0 34 44323.5 35 48549.0 36 53625.0 37 58841.5 38 62141.5 39 64242.0 40 66105.5 41 69428.0 42 72330.0 43 74940.0 44 78122.5 45 80517.5 46 81781.0 47 83016.5 48 84335.0 49 82710.0 50 79208.5 51 75297.0 52 71446.5 53 69336.0 54 67737.5 55 62366.5 56 54943.5 57 48919.5 58 44100.5 59 38894.5 60 32392.0 61 26407.5 62 21146.5 63 16162.5 64 12136.0 65 9333.5 66 7048.0 67 5391.0 68 3975.0 69 2732.5 70 2034.5 71 1541.5 72 1186.5 73 999.0 74 795.0 75 602.5 76 458.0 77 295.0 78 187.0 79 135.0 80 85.0 81 57.5 82 41.5 83 25.5 84 17.0 85 9.0 86 5.0 87 4.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018947094732707663 2 0.0 3 0.0 4 1.8439994873681426E-4 5 0.0232804935280228 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002120599410473364 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.297997693156642E-4 24-25 0.0 26-27 4.840498654341374E-4 28-29 0.0011294496860129873 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.3049993592101782E-5 40-41 7.145498013551552E-4 42-43 0.013276796309050628 44-45 2.3049993592101782E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.6099987184203564E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.914998077630534E-5 82-83 0.0020514494296970586 84-85 4.609998718420356E-4 86-87 0.0 88-89 0.0 90-91 0.010672147033143124 92-93 0.0021666993976575673 94-95 0.0 96-97 0.0 98-99 0.0018900994745523459 100-101 0.018831844764747155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2169198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.7870869954075 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33664453829628 45.26846492862841 2 12.598212948840267 14.560280553226038 3 3.8321472125188847 6.643458730471114 4 1.6901594626781202 3.906775676235669 5 0.9064280134135692 2.6189917233102156 6 0.556206487180667 1.9284931597271529 7 0.36226103257717546 1.4653806863208372 8 0.2579128199454217 1.1923224450733563 9 0.19478192566202904 1.01302920750282 >10 1.1447437161176977 12.15838766209156 >50 0.08136632374354612 3.216006336579507 >100 0.036816376651528135 3.788115434710135 >500 0.0018393198145676198 0.7622302955009946 >1k 2.3991128016098291E-4 0.25208962422443254 >5k 1.599408534406553E-4 0.730204977519754 >10k+ 7.997042672032765E-5 0.4957685588780407 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10728 0.4945606625121358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9326 0.4299284804798824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6475 0.29849741701771804 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.1113314690498516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.6099987184203564E-5 0.0 0.0 0.0 8 0.0 4.6099987184203564E-5 0.0 0.0 0.0 9 0.0 4.6099987184203564E-5 0.0 0.0 0.0 10-11 0.0 4.6099987184203564E-5 0.0 4.6099987184203564E-5 0.0 12-13 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 1.8439994873681426E-4 14-15 0.0 4.6099987184203564E-5 0.0 9.219997436840713E-5 1.8439994873681426E-4 16-17 0.0 4.6099987184203564E-5 0.0 1.3829996155261069E-4 1.8439994873681426E-4 18-19 0.0 4.6099987184203564E-5 0.0 1.3829996155261069E-4 1.8439994873681426E-4 20-21 0.0 4.6099987184203564E-5 0.0 1.3829996155261069E-4 1.8439994873681426E-4 22-23 0.0 4.6099987184203564E-5 0.0 1.8439994873681426E-4 2.3049993592101783E-4 24-25 0.0 4.6099987184203564E-5 0.0 1.8439994873681426E-4 2.3049993592101783E-4 26-27 0.0 4.6099987184203564E-5 0.0 2.535499295131196E-4 2.3049993592101783E-4 28-29 0.0 4.6099987184203564E-5 0.0 8.297997693156641E-4 2.3049993592101783E-4 30-31 0.0 4.6099987184203564E-5 0.0 0.0024893993079469923 2.3049993592101783E-4 32-33 0.0 4.6099987184203564E-5 0.0 0.005278448532591308 2.7659992310522137E-4 34-35 0.0 6.914998077630534E-5 0.0 0.009243047430432815 3.226999102894249E-4 36-37 0.0 9.219997436840713E-5 0.0 0.015189945777195074 3.226999102894249E-4 38-39 0.0 9.219997436840713E-5 0.0 0.025977342778298707 3.226999102894249E-4 40-41 0.0 9.219997436840713E-5 0.0 0.03704133970250756 3.226999102894249E-4 42-43 0.0 9.219997436840713E-5 0.0 0.048151436613900624 3.226999102894249E-4 44-45 0.0 9.219997436840713E-5 0.0 0.061773982826832774 3.226999102894249E-4 46-47 4.6099987184203564E-5 9.219997436840713E-5 0.0 0.07940722792479063 3.457499038815267E-4 48-49 4.6099987184203564E-5 9.219997436840713E-5 0.0 0.09925327240759027 3.687998974736285E-4 50-51 4.6099987184203564E-5 9.219997436840713E-5 0.0 0.12269511589075778 3.687998974736285E-4 52-53 4.6099987184203564E-5 9.219997436840713E-5 0.0 0.14712810909838567 3.687998974736285E-4 54-55 4.6099987184203564E-5 1.6134995514471248E-4 0.0 0.17755410063996002 3.687998974736285E-4 56-57 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.2105616914638498 3.687998974736285E-4 58-59 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.2522130298847777 3.687998974736285E-4 60-61 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.29739101732529716 3.687998974736285E-4 62-63 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.3465566536572503 3.687998974736285E-4 64-65 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.3977967894124925 3.687998974736285E-4 66-67 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.46017007207272 3.687998974736285E-4 68-69 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.5318094521569723 3.687998974736285E-4 70-71 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.612576629703697 3.687998974736285E-4 72-73 4.6099987184203564E-5 1.8439994873681426E-4 0.0 0.6985992057894208 4.1489988465783206E-4 74-75 9.219997436840713E-5 1.8439994873681426E-4 0.0 0.7932424794785907 4.6099987184203565E-4 76-77 9.219997436840713E-5 1.8439994873681426E-4 0.0 0.8986731501688643 4.6099987184203565E-4 78-79 9.219997436840713E-5 1.8439994873681426E-4 0.0 1.017495867136149 4.6099987184203565E-4 80-81 1.3829996155261069E-4 1.8439994873681426E-4 0.0 1.1539979291885758 4.6099987184203565E-4 82-83 1.3829996155261069E-4 1.8439994873681426E-4 0.0 1.2927819406066205 4.6099987184203565E-4 84-85 1.3829996155261069E-4 1.8439994873681426E-4 0.0 1.4386653500510327 4.6099987184203565E-4 86-87 1.3829996155261069E-4 1.8439994873681426E-4 0.0 1.5978716557916797 4.840498654341374E-4 88-89 1.3829996155261069E-4 1.8439994873681426E-4 0.0 1.7744576567007715 5.070998590262391E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6300 0.0 45.178528 1 GTATCAA 10385 0.0 37.610676 1 ATCAACG 13365 0.0 29.005268 3 TATCAAC 13555 0.0 28.668797 2 TCAACGC 13530 0.0 28.651546 4 CAACGCA 13845 0.0 28.033981 5 AACGCAG 14330 0.0 27.080175 6 ACGCAGA 16530 0.0 23.390375 7 CGCAGAG 16585 0.0 23.255527 8 GCAGAGT 17510 0.0 21.782866 9 GAGTACT 10830 0.0 21.490833 12-13 AGTACTT 11200 0.0 20.547613 12-13 AGAGTAC 15725 0.0 20.37403 10-11 TGCACCG 350 3.6379788E-12 20.360054 5 GTACTTT 11395 0.0 20.174679 14-15 GTACCGT 215 1.0801286E-6 19.882896 6 GTACATG 8710 0.0 19.1485 1 TACATGG 8595 0.0 19.01377 2 CAGAGTA 17275 0.0 18.99965 10-11 GCACCGT 350 6.366463E-11 18.999212 6 >>END_MODULE