FastQCFastQC Report
Thu 26 May 2016
SRR937525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109277
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101660.48196609549148833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76730.3637739377047206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.24866340456943303No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28840.1367293153056711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.048.252191
GTATCAA87000.038.468071
ATCAACG109300.030.5637473
TATCAAC111150.030.1020852
TCAACGC111000.030.0963734
CAACGCA111950.029.7985275
AACGCAG116600.028.6020036
GTACATG84550.026.3135381
TACATGG85550.025.2214012
ACGCAGA133100.024.8778387
CGCAGAG134000.024.6752958
ACATGGG84700.024.2366033
GCAGAGT143900.022.7130379
GAGTACT85850.022.13122212-13
GTACTTT88350.021.45045514-15
TAACGCA2108.578918E-720.3659654
ATAACGC2351.12810085E-720.2210453
AGTACTT89050.020.05578212-13
CAGAGTA141100.019.9135910-11
TATAACG2401.4063698E-719.8025972
Produced by FastQC (version 0.11.5)