##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937525_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2109277 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950832915733685 33.0 31.0 34.0 30.0 34.0 2 32.10313344335523 33.0 31.0 34.0 31.0 34.0 3 32.12921015115606 34.0 31.0 34.0 30.0 34.0 4 35.728388921891245 37.0 35.0 37.0 35.0 37.0 5 35.72587479027174 37.0 35.0 37.0 35.0 37.0 6 35.78657900313709 37.0 35.0 37.0 35.0 37.0 7 35.73810362508101 37.0 35.0 37.0 35.0 37.0 8 35.720148183477086 37.0 35.0 37.0 35.0 37.0 9 37.383483060783384 39.0 37.0 39.0 35.0 39.0 10-11 37.28199164927129 39.0 37.0 39.0 35.0 39.0 12-13 37.16678748215621 39.0 37.0 39.0 34.0 39.0 14-15 38.36584384127832 40.0 38.0 41.0 34.0 41.0 16-17 38.29015653230941 40.0 38.0 41.0 34.0 41.0 18-19 38.21972742318813 40.0 38.0 41.0 33.5 41.0 20-21 38.13311291025313 40.0 38.0 41.0 34.0 41.0 22-23 37.93569716068586 40.0 38.0 41.0 33.0 41.0 24-25 37.76842301888277 40.0 37.5 41.0 33.0 41.0 26-27 37.71920046537274 40.0 37.5 41.0 33.0 41.0 28-29 37.76703913236621 40.0 38.0 41.0 33.0 41.0 30-31 37.59149201361414 40.0 37.0 41.0 33.0 41.0 32-33 37.35381673435968 40.0 37.0 41.0 32.5 41.0 34-35 37.378629739005355 40.0 37.0 41.0 33.0 41.0 36-37 37.28670487565171 40.0 37.0 41.0 32.0 41.0 38-39 37.15487510649383 40.0 37.0 41.0 32.0 41.0 40-41 36.9222299868628 39.5 36.0 41.0 31.0 41.0 42-43 36.677094568423215 39.0 36.0 41.0 31.0 41.0 44-45 36.442107888153146 39.0 36.0 41.0 30.0 41.0 46-47 36.10537520676516 39.0 35.5 41.0 29.5 41.0 48-49 35.75176233372857 39.0 35.0 40.0 28.5 41.0 50-51 34.82559118598458 38.0 34.0 39.5 26.5 40.5 52-53 34.93659391345945 38.0 34.5 39.5 27.0 40.5 54-55 35.28483480358435 39.0 35.0 40.0 27.0 41.0 56-57 35.0823331881019 38.0 35.0 40.0 26.5 41.0 58-59 34.83440249905536 38.0 34.0 40.0 26.0 41.0 60-61 34.51003092528862 38.0 34.0 40.0 25.5 41.0 62-63 34.14867298131065 37.5 34.0 40.0 24.5 41.0 64-65 33.77643998393762 37.0 33.5 40.0 23.0 41.0 66-67 33.356745937114944 36.5 33.0 39.0 22.5 41.0 68-69 32.867235313332486 36.0 32.0 39.0 21.5 40.0 70-71 32.41456930502727 35.0 32.0 38.5 19.5 40.0 72-73 31.954996901781982 35.0 31.5 38.0 18.0 40.0 74-75 31.49067026284362 35.0 31.0 37.0 16.0 39.0 76-77 30.989297280537357 35.0 31.0 36.5 10.5 39.0 78-79 30.4576264758019 34.0 30.0 36.0 7.0 38.5 80-81 29.922603100493674 34.0 29.5 35.5 4.5 37.5 82-83 29.393825467209854 34.0 29.0 35.0 2.0 37.0 84-85 28.91641970210646 34.0 29.0 35.0 2.0 36.5 86-87 28.435512263206775 34.0 28.5 35.0 2.0 36.0 88-89 27.942456348786813 33.5 27.0 35.0 2.0 36.0 90-91 27.424681774845126 33.0 26.0 35.0 2.0 35.0 92-93 26.838040238432413 33.0 25.0 35.0 2.0 35.0 94-95 26.18084419447991 32.0 24.5 34.5 2.0 35.0 96-97 25.436879793407883 32.0 21.5 34.0 2.0 35.0 98-99 24.470581151740618 31.5 10.0 34.0 2.0 35.0 100-101 22.04813924392102 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1370489920265463 1101 2 0.1986396183655259 1101 3 0.2248167909804124 1101 4 0.1319536172403062 1101 5 0.11866478345629616 1101 6 0.15183569705991573 1101 7 0.10899027669187689 1101 8 0.01084232844867472 1101 9 0.09533895093797895 1101 10-11 0.1431688976242711 1101 12-13 0.20691743006382524 1101 14-15 0.2951583673947127 1101 16-17 0.48454345006256716 1101 18-19 0.4385743895456642 1101 20-21 0.39842608402083357 1101 22-23 0.35835524157852916 1101 24-25 0.31096463895273274 1101 26-27 0.35585391528562127 1101 28-29 0.1465235880609299 1101 30-31 0.2364432156069256 1101 32-33 0.36249702754216884 1101 34-35 0.4843382075358349 1101 36-37 0.4710513594054575 1101 38-39 0.5460236285901274 1101 40-41 0.5373233307597118 1101 42-43 0.48078425986383166 1101 44-45 0.4785813203728395 1101 46-47 0.5379107117517208 1101 48-49 0.7550605955411029 1101 50-51 0.6450105070718166 1101 52-53 0.7837641983205899 1101 54-55 0.7681978628319186 1101 56-57 0.7249846387695484 1101 58-59 0.7712633882257549 1101 60-61 0.8406929902999778 1101 62-63 1.000034743076263 1101 64-65 0.8261217263170977 1101 66-67 0.8309093773972052 1101 68-69 0.7352519403139652 1101 70-71 0.7798474055703437 1101 72-73 0.7752225755436726 1101 74-75 0.8581340566907905 1101 76-77 0.9177122444755206 1101 78-79 1.0546009915681758 1101 80-81 0.9689353419984741 1101 82-83 1.020550420235633 1101 84-85 1.301958861133265 1101 86-87 1.350517924737769 1101 88-89 1.475699549173445 1101 90-91 1.7040225202552897 1101 92-93 1.599735425586811 1101 94-95 1.773213723754111 1101 96-97 1.775183268036848 1101 98-99 1.6882202484029953 1101 100-101 2.0446407616213627 1102 1 0.18579455236811881 1102 2 0.10441375052823787 1102 3 0.12246557535556235 1102 4 0.115098715926095 1102 5 0.07298044059444209 1102 6 0.015092843450140947 1102 7 0.1119797039501762 1102 8 0.18932724563229897 1102 9 0.22362086323711594 1102 10-11 0.14552371993487867 1102 12-13 0.1130611616439623 1102 14-15 0.19004168252286746 1102 16-17 0.2178307552156653 1102 18-19 0.22268504953481738 1102 20-21 0.33493501829671146 1102 22-23 0.431161689645684 1102 24-25 0.3052476985214412 1102 26-27 0.28256657578314304 1102 28-29 0.06298931080640813 1102 30-31 0.18601522222948574 1102 32-33 0.23716212100685397 1102 34-35 0.3742702300965206 1102 36-37 0.4480226707963979 1102 38-39 0.3793581641520447 1102 40-41 0.40721008082669385 1102 42-43 0.37757997718210845 1102 44-45 0.5174728999638063 1102 46-47 0.4858344629797031 1102 48-49 0.3847683509459827 1102 50-51 0.3802608797626732 1102 52-53 0.4176871389030197 1102 54-55 0.4309164108145822 1102 56-57 0.4808068600757949 1102 58-59 0.2741544044867581 1102 60-61 0.5131483876193457 1102 62-63 0.5436952199831708 1102 64-65 0.40698568213004904 1102 66-67 0.5774321888259308 1102 68-69 0.5420156783517882 1102 70-71 0.6133993727598082 1102 72-73 0.7831521318182837 1102 74-75 0.7633998179075867 1102 76-77 0.7565485215514265 1102 78-79 0.8872925065822947 1102 80-81 0.9918085996257595 1102 82-83 1.031915774068679 1102 84-85 0.9899348577807636 1102 86-87 1.0410149947349545 1102 88-89 1.0786791868560996 1102 90-91 1.0320335876281774 1102 92-93 1.2960554038779968 1102 94-95 1.5138265511896556 1102 96-97 1.396850175149229 1102 98-99 1.4743010175544953 1102 100-101 1.9032473443381228 1103 1 0.2369621920678675 1103 2 0.22587102553013239 1103 3 0.278895175780157 1103 4 0.14727325531498536 1103 5 0.1439372985821592 1103 6 0.09945102071907996 1103 7 0.09594740430653559 1103 8 0.17045545853699196 1103 9 0.1335988752167978 1103 10-11 0.15455965897499624 1103 12-13 0.2565140390417895 1103 14-15 0.32333836915567105 1103 16-17 0.2858230214981674 1103 18-19 0.2253198209352263 1103 20-21 0.30859488636858856 1103 22-23 0.37177318407285753 1103 24-25 0.35474159495029056 1103 26-27 0.27473997786062654 1103 28-29 0.2091717355543068 1103 30-31 0.25712372173665443 1103 32-33 0.16154039077711246 1103 34-35 0.19212791453050926 1103 36-37 0.21452316363133406 1103 38-39 0.2012912144212109 1103 40-41 0.23409422670584235 1103 42-43 0.10755761005798803 1103 44-45 0.09892145557833487 1103 46-47 0.23030902511482054 1103 48-49 0.3748585776500519 1103 50-51 0.38189102609673853 1103 52-53 0.18562443513473426 1103 54-55 0.34518966883262436 1103 56-57 0.27866871465157317 1103 58-59 0.2566985700793012 1103 60-61 0.3032291367284543 1103 62-63 0.5240348969867341 1103 64-65 0.4062919221785748 1103 66-67 0.41905778591441845 1103 68-69 0.49386191385107736 1103 70-71 0.5506216792273122 1103 72-73 0.5571683574608528 1103 74-75 0.5729873529746818 1103 76-77 0.6590183827994345 1103 78-79 0.4517799582465578 1103 80-81 0.486425325685353 1103 82-83 0.7443371100304645 1103 84-85 0.8499090787224581 1103 86-87 0.8196581822396958 1103 88-89 0.6796288418716436 1103 90-91 0.8656961094266471 1103 92-93 1.026795035389103 1103 94-95 1.1640672821017795 1103 96-97 1.3202067601822094 1103 98-99 1.5966700371920695 1103 100-101 1.8902971585772654 1104 1 0.16927712974790765 1104 2 0.2587769267718869 1104 3 0.2187288701685972 1104 4 0.2042389994307996 1104 5 0.15960953079565599 1104 6 0.16618739810708405 1104 7 0.11270262323451163 1104 8 0.1564746786936908 1104 9 0.21236562052382624 1104 10-11 0.26283529195250566 1104 12-13 0.2429034820644418 1104 14-15 0.4033152592478757 1104 16-17 0.4875283148100422 1104 18-19 0.4426002812760146 1104 20-21 0.41438004280694685 1104 22-23 0.40559892751002735 1104 24-25 0.3975747553462625 1104 26-27 0.4780543029673865 1104 28-29 0.4022702848450237 1104 30-31 0.5242388051484141 1104 32-33 0.4416204182089629 1104 34-35 0.5259527650037015 1104 36-37 0.618108235416706 1104 38-39 0.6508709855724035 1104 40-41 0.6141070139500258 1104 42-43 0.6132883849899926 1104 44-45 0.6517275055878855 1104 46-47 0.7555687436882863 1104 48-49 0.8745501478914903 1104 50-51 0.8246306038676607 1104 52-53 0.8196271640326458 1104 54-55 0.8798130076963844 1104 56-57 0.947395955232146 1104 58-59 0.9265483556786407 1104 60-61 0.9724370598192493 1104 62-63 1.0329425698690287 1104 64-65 0.886781105705893 1104 66-67 0.9477172862474887 1104 68-69 0.9873888179628096 1104 70-71 1.0131175492689053 1104 72-73 1.0674080647115431 1104 74-75 1.2339901872423589 1104 76-77 1.1999285452653048 1104 78-79 1.3676307832559793 1104 80-81 1.2946746162254712 1104 82-83 1.3048638159042056 1104 84-85 1.4814774985155417 1104 86-87 1.5606829119841414 1104 88-89 1.5773373177071441 1104 90-91 1.644344894191967 1104 92-93 1.7647951175417909 1104 94-95 1.8755662471565273 1104 96-97 1.8931762940371542 1104 98-99 2.097446380075951 1104 100-101 2.5345372730195272 1105 1 0.1985165420458479 1105 2 0.15554923673504106 1105 3 0.21972707152996662 1105 4 0.12225687860708234 1105 5 0.1354277486405593 1105 6 0.12453804935977075 1105 7 0.1054263211359725 1105 8 0.1907784217573507 1105 9 0.18615776145898622 1105 10-11 0.2721498278051442 1105 12-13 0.2869141303114162 1105 14-15 0.43338947343232803 1105 16-17 0.4481477012435846 1105 18-19 0.42474599302096294 1105 20-21 0.3853689303025334 1105 22-23 0.42495013491440403 1105 24-25 0.4140400044802064 1105 26-27 0.3023561219950395 1105 28-29 0.4215522843874666 1105 30-31 0.41760231681085713 1105 32-33 0.37073414596648746 1105 34-35 0.3822915063778396 1105 36-37 0.24127209723454968 1105 38-39 0.30938149454214425 1105 40-41 0.42127878633178284 1105 42-43 0.544788587444522 1105 44-45 0.4251494849710298 1105 46-47 0.4375097992661239 1105 48-49 0.5292039697831896 1105 50-51 0.36171663699362 1105 52-53 0.31330617930911586 1105 54-55 0.3167759408078936 1105 56-57 0.3316909396244796 1105 58-59 0.24292927268760423 1105 60-61 0.3166554273752169 1105 62-63 0.42079079884653936 1105 64-65 0.49366944174020944 1105 66-67 0.4080886539116193 1105 68-69 0.49370606948217954 1105 70-71 0.5944721088991898 1105 72-73 0.7083591128325466 1105 74-75 0.8849208558975086 1105 76-77 0.8395220471926912 1105 78-79 0.8675783269512216 1105 80-81 0.940606428772675 1105 82-83 1.0499838958522076 1105 84-85 1.1313565522727238 1105 86-87 1.1291119700967336 1105 88-89 1.1609249077706956 1105 90-91 1.2829877691803588 1105 92-93 1.5987313793025812 1105 94-95 1.615418393126724 1105 96-97 1.833733249810173 1105 98-99 2.0663314634364873 1105 100-101 2.44634520636556 1106 1 0.0949482573241589 1106 2 0.15491480005353253 1106 3 0.21022477297371012 1106 4 0.1719324869275809 1106 5 0.11044410242772784 1106 6 0.042638710779606015 1106 7 0.09590264508015167 1106 8 0.016567477142650944 1106 9 0.12214450170858981 1106 10-11 -7.548565750781222E-4 1106 12-13 -0.03506462330435056 1106 14-15 0.04965076728411333 1106 16-17 0.05756736388763528 1106 18-19 0.24599636772101974 1106 20-21 0.33974043894118466 1106 22-23 0.31879244117606476 1106 24-25 0.13787893309693544 1106 26-27 0.12233370412383238 1106 28-29 0.24672649913252798 1106 30-31 0.3447144256441774 1106 32-33 0.407763775655944 1106 34-35 0.4485100927360506 1106 36-37 0.3955321340893576 1106 38-39 0.5286083463917919 1106 40-41 0.5728482273386746 1106 42-43 0.5451643563813136 1106 44-45 0.4942320624513954 1106 46-47 0.5921406169744898 1106 48-49 0.5105330991916475 1106 50-51 0.5463581387012084 1106 52-53 0.5413548126455652 1106 54-55 0.7816282436634054 1106 56-57 0.7230473989784869 1106 58-59 0.8284199124306539 1106 60-61 0.7662190175980044 1106 62-63 0.9293281408252554 1106 64-65 0.8505651342690612 1106 66-67 0.9405705768604662 1106 68-69 0.9551562620459464 1106 70-71 0.997299259463766 1106 72-73 1.0181513852940043 1106 74-75 1.0765412798655838 1106 76-77 1.1751319258777535 1106 78-79 1.2345973343986678 1106 80-81 1.1350534332386744 1106 82-83 0.9797436080065509 1106 84-85 1.002140968740619 1106 86-87 1.144827757665194 1106 88-89 1.340189493282356 1106 90-91 1.5150793627399395 1106 92-93 1.5000148368708572 1106 94-95 1.9759116813044422 1106 96-97 2.0629329427342675 1106 98-99 2.305940692245567 1106 100-101 2.361938643378185 1107 1 0.06617149250115162 1107 2 0.18730026534379363 1107 3 0.1550094173229084 1107 4 0.179914277304853 1107 5 0.09943680019935641 1107 6 0.04111582268819802 1107 7 0.1892121167701717 1107 8 0.1685249880229449 1107 9 0.11251423150964257 1107 10-11 0.2511828612370337 1107 12-13 0.26627623924928656 1107 14-15 0.44451658677603945 1107 16-17 0.3449220674511011 1107 18-19 0.4844971841850878 1107 20-21 0.48006704812940626 1107 22-23 0.527125205065964 1107 24-25 0.5076413691523669 1107 26-27 0.4244684100907321 1107 28-29 0.483096144106149 1107 30-31 0.5723284655272067 1107 32-33 0.540906325804265 1107 34-35 0.506940054728652 1107 36-37 0.5552339185287067 1107 38-39 0.6862166604229571 1107 40-41 0.6946874039470359 1107 42-43 0.7102397232010631 1107 44-45 0.579048187204279 1107 46-47 0.47535774457615787 1107 48-49 0.7106099151467404 1107 50-51 0.7378299182629746 1107 52-53 0.7533200758109047 1107 54-55 0.6878807325239151 1107 56-57 0.6592083920250005 1107 58-59 0.8858203992458797 1107 60-61 0.9192913023542744 1107 62-63 0.9720362802532776 1107 64-65 0.9206321243795941 1107 66-67 0.7630802436535902 1107 68-69 0.8595121508371761 1107 70-71 1.0332189655838349 1107 72-73 0.9684877496372621 1107 74-75 0.9850990720902111 1107 76-77 1.0833425146979714 1107 78-79 1.3879828915853167 1107 80-81 1.3587406697729065 1107 82-83 1.266792814987852 1107 84-85 1.356735905956537 1107 86-87 1.452388709711773 1107 88-89 1.4453441737539023 1107 90-91 1.5444151639232828 1107 92-93 1.5069655026484092 1107 94-95 1.6355913163579672 1107 96-97 1.845106575068602 1107 98-99 2.2266543737326856 1107 100-101 2.802622470320788 1108 1 0.2176414121329593 1108 2 0.18637457719798078 1108 3 0.2187979639305695 1108 4 0.16415979250366775 1108 5 0.14316077646161318 1108 6 0.18362643202961237 1108 7 0.19693737604578843 1108 8 0.18455106397054521 1108 9 0.19294737841966736 1108 10-11 0.2594326171747241 1108 12-13 0.2653000899081235 1108 14-15 0.2456510144011972 1108 16-17 0.21911381399799978 1108 18-19 0.3110736468852693 1108 20-21 0.47638098271370666 1108 22-23 0.3729425715111674 1108 24-25 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64-65 -0.3820926458888678 2316 66-67 -0.4008936609387206 2316 68-69 -0.447424897774539 2316 70-71 -0.5986518842628215 2316 72-73 -0.4265754228985408 2316 74-75 -0.5301979348526586 2316 76-77 -0.6295835580130067 2316 78-79 -0.608160412009493 2316 80-81 -0.5944877930772208 2316 82-83 -0.6315898982594703 2316 84-85 -0.5019915646395745 2316 86-87 -0.6748648126444969 2316 88-89 -0.7538119738562479 2316 90-91 -0.5461528942268785 2316 92-93 -0.4409757885039447 2316 94-95 -0.29078343557852904 2316 96-97 -0.09341033980706115 2316 98-99 -0.19483359991584237 2316 100-101 0.01815808380147743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7930.0 3 1373.0 4 1270.0 5 1428.0 6 1542.0 7 1931.0 8 2295.0 9 2864.0 10 3550.0 11 4708.0 12 6316.0 13 8400.0 14 9952.0 15 12507.0 16 15840.0 17 20428.0 18 18172.0 19 12612.0 20 12580.0 21 13226.0 22 14108.0 23 15659.0 24 17590.0 25 20357.0 26 23339.0 27 28017.0 28 33766.0 29 40983.0 30 51506.0 31 64536.0 32 83674.0 33 111666.0 34 155163.0 35 221472.0 36 315763.0 37 414519.0 38 302914.0 39 35313.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.292582320263875 19.058987768990207 14.623148623197125 23.02528128754879 2 15.205431781359579 19.739269170210015 40.992273726486495 24.06302532194391 3 19.475193075841833 24.136536124089524 29.415645809991602 26.972624990077037 4 12.498389964595855 16.274784445370727 37.22173716522551 34.0050884248079 5 13.584641122401454 37.30468712869405 34.17388287406253 14.936788874841971 6 32.0695799898621 35.98696990412704 17.38055547505835 14.562894630952512 7 28.518381816791287 32.737414085735416 20.39179137268538 18.352412724787914 8 26.207367704425273 35.15102221542324 19.530816114293913 19.11079396585758 9 26.039032394920913 17.08996340386352 20.47668570033166 36.394318500883905 10-11 24.865861419470885 26.906107625855803 26.96062536478781 21.2674055898855 12-13 25.58265928989858 24.08545978388961 27.92904544237686 22.402835483834956 14-15 22.579360941359568 25.447070021195223 26.359583373615266 25.613985663829947 16-17 21.800834393506516 28.859687544239865 27.048454110732433 22.291023951521186 18-19 21.684654432752946 27.176875199970734 29.689944705344978 21.448525661931345 20-21 22.295349137677153 26.24558008477877 30.017874412657154 21.44119636488692 22-23 21.95726728949679 26.148700751096175 29.9483777229819 21.945654236425135 24-25 21.803505623783927 26.408237719546772 29.9636622884565 21.8245943682128 26-27 21.505817402948672 26.656117927994643 29.90382153472272 21.934243134333958 28-29 21.476342189729973 26.884462696522878 29.984549923889848 21.6546451898573 30-31 21.68249912824864 26.539508151706592 29.912045751031613 21.86594696901316 32-33 21.394383517941943 26.573876581252375 29.916856643807648 22.114883256998034 34-35 21.90096327013036 26.641138765150114 29.746196824063652 21.71170114065587 36-37 21.882543440240994 26.443115632596875 29.672503664550426 22.001837262611705 38-39 21.615501741017447 26.718163701003377 29.76540143152699 21.900933126452184 40-41 21.89872254344732 26.63173101288143 29.640668292806883 21.828878150864366 42-43 21.652870223814944 26.580613472219316 29.549738448705355 22.216777855260382 44-45 21.742635190531697 26.816394912821213 29.45679156608307 21.98417833056402 46-47 21.900924293966188 27.11715141610519 28.902183302192615 22.07974098773601 48-49 21.945349153173627 27.07906183077154 28.819507124818767 22.156081891236067 50-51 22.0756764820534 27.2772795481275 28.66821563523904 21.97882833458006 52-53 22.132303311324677 27.3427271049091 28.473206036158317 22.05176354760791 54-55 21.898685049966772 27.271876524572004 28.660694817678404 22.16874360778282 56-57 21.959533424295426 27.57379113893692 28.429042205240307 22.037633231527344 58-59 22.05175940910165 27.189991487017405 28.5789493056042 22.179299798276745 60-61 21.933877298809833 27.26946951610915 28.77521801008756 22.02143517499345 62-63 21.751893257942633 27.398073290759818 28.874870297551485 21.97516315374606 64-65 21.774431590833263 27.305202091142565 28.73900968750408 22.181356630520092 66-67 21.683512382141142 27.413477949769444 28.59819946647516 22.304810201614252 68-69 21.760458148407103 27.25200727292072 28.757865697957197 22.229668880714982 70-71 21.877600357163214 27.291116514998954 28.573626916427607 22.257656211410225 72-73 21.86489518540613 27.34835416199621 28.4982369413973 22.288513711200363 74-75 21.749509535354658 27.6765263062651 28.326758577876955 22.24720558050329 76-77 21.89292136563463 27.44717453879631 28.33945294714093 22.320451148428127 78-79 21.941697484113448 27.456652684900522 28.316223702240872 22.285426128745154 80-81 21.821338937872127 27.717220015119715 28.233062107919988 22.228378939088167 82-83 22.026491670585553 27.64945206845938 28.200969983706937 22.123086277248134 84-85 21.96841956269587 27.646419556996115 28.132513582038936 22.25264729826908 86-87 21.911599614599343 27.569256579039553 28.22523091859444 22.293912887766666 88-89 21.95924827430274 27.591847184828683 28.078324710388763 22.370579830479816 90-91 21.978570062926543 27.61212835161912 28.111479373418373 22.29782221203596 92-93 22.00619487395792 27.410916031005723 28.293621445796752 22.289267649239605 94-95 21.830635854761518 27.434006636208156 28.218348395940946 22.517009113089376 96-97 21.99096655917094 27.386595171716117 28.092386167721834 22.530052101391114 98-99 21.861594461555562 27.39023058565141 27.99570999020831 22.75246496258472 100-101 22.15011304773414 26.90194115343597 28.409497576971845 22.538448221858047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2867.0 1 1727.0 2 674.0 3 735.5 4 575.0 5 492.5 6 943.0 7 2023.0 8 3285.5 9 3803.0 10 3224.5 11 2607.5 12 2592.0 13 2779.5 14 3001.5 15 3159.5 16 3209.0 17 3363.5 18 3593.5 19 3837.5 20 4237.0 21 4947.5 22 5834.0 23 7026.5 24 8540.5 25 10651.0 26 13554.0 27 16819.5 28 19573.0 29 22486.0 30 26247.0 31 29972.0 32 34517.0 33 38373.0 34 42132.5 35 46322.5 36 51016.5 37 55806.0 38 58912.5 39 61476.5 40 63655.5 41 67150.0 42 70064.0 43 72433.5 44 75766.5 45 78357.0 46 79461.0 47 80713.5 48 82335.0 49 81032.0 50 77381.5 51 73292.0 52 70202.0 53 68255.0 54 66411.0 55 61643.0 56 54705.0 57 48946.0 58 44592.5 59 39693.5 60 33136.5 61 27019.0 62 21670.5 63 16282.5 64 12171.5 65 9471.0 66 7007.5 67 5251.0 68 3916.0 69 2780.0 70 2076.0 71 1583.5 72 1253.5 73 1044.0 74 832.5 75 641.0 76 463.5 77 309.0 78 208.5 79 162.0 80 112.5 81 84.0 82 52.5 83 33.5 84 26.0 85 22.0 86 16.5 87 12.0 88 10.5 89 8.5 90 5.0 91 4.5 92 6.5 93 4.0 94 2.0 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29299138994072377 2 0.2752127861821847 3 0.2640715278268336 4 0.2506071985803666 5 0.24733593548879546 6 0.20405096153800567 7 0.19561205095395248 8 0.2080333687799184 9 0.20907638020041938 10-11 0.211328336676501 12-13 0.20767779670474765 14-15 0.20488062971340415 16-17 0.20267608284734534 18-19 0.20298424531249334 20-21 0.19120295722183478 22-23 0.1843048589635216 24-25 0.1843522685735444 26-27 0.19570687017399802 28-29 0.21016680123094308 30-31 0.2047621056883472 32-33 0.20947936188561292 34-35 0.20196493869700377 36-37 0.188263561400423 38-39 0.1916533485170511 40-41 0.18406781091340776 42-43 0.19651283354438512 44-45 0.1926252455225179 46-47 0.19193780617718773 48-49 0.20106415610657113 50-51 0.21028532525600002 52-53 0.22408152177262633 54-55 0.19807735067513657 56-57 0.17247616126284032 58-59 0.17396956397855756 60-61 0.18074913821181382 62-63 0.188571723865571 64-65 0.17681414057992384 66-67 0.18231365534256525 68-69 0.1864619962195577 70-71 0.18001428925646087 72-73 0.18228995053755387 74-75 0.18373594364324836 76-77 0.19724768249973806 78-79 0.20260496843231116 80-81 0.19336009447787086 82-83 0.1937630761630644 84-85 0.18598790011932997 86-87 0.18658052024461463 88-89 0.19158223410201694 90-91 0.19174816773709663 92-93 0.1890695247708101 94-95 0.1900414217762769 96-97 0.17859200095577774 98-99 0.18586937609427306 100-101 0.177809742390402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2109277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.1149714788958 #Duplication Level Percentage of deduplicated Percentage of total 1 78.57314437277394 45.66276044230919 2 12.404194680003414 14.417388400941386 3 3.872203886579494 6.750990552871102 4 1.651304717168549 3.8386210616486527 5 0.8925795235154531 2.5936116775873477 6 0.5368233544734735 1.871848436065865 7 0.36833659258073503 1.498410940072415 8 0.26360432546511603 1.2255486284895025 9 0.2021357429895848 1.057240164483462 >10 1.1156255023951307 12.008080505689103 >50 0.08188443118683528 3.270898177744223 >100 0.035876303395942055 3.6832060071968673 >500 0.0017965887282586843 0.7123521034354099 >1k 2.449893720352715E-4 0.3135108591135419 >5k 1.6332624802351434E-4 0.6130689188659906 >10k+ 8.166312401175717E-5 0.48246312348596226 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10166 0.48196609549148833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7673 0.3637739377047206 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.24866340456943303 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2884 0.1367293153056711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.7409610022770836E-5 10-11 0.0 0.0 0.0 0.0 4.7409610022770836E-5 12-13 0.0 0.0 0.0 0.0 1.422288300683125E-4 14-15 0.0 0.0 0.0 2.3704805011385418E-5 1.422288300683125E-4 16-17 0.0 0.0 0.0 9.481922004554167E-5 1.422288300683125E-4 18-19 0.0 0.0 0.0 1.1852402505692708E-4 1.422288300683125E-4 20-21 0.0 0.0 0.0 1.8963844009108334E-4 1.422288300683125E-4 22-23 0.0 0.0 0.0 3.792768801821667E-4 1.422288300683125E-4 24-25 0.0 0.0 0.0 5.215057102504792E-4 1.422288300683125E-4 26-27 0.0 0.0 0.0 5.452105152618646E-4 1.422288300683125E-4 28-29 4.7409610022770836E-5 0.0 0.0 0.0012800594706148126 1.422288300683125E-4 30-31 4.7409610022770836E-5 0.0 0.0 0.00284457660136625 1.422288300683125E-4 32-33 4.7409610022770836E-5 0.0 0.0 0.005760267617766657 1.422288300683125E-4 34-35 4.7409610022770836E-5 0.0 0.0 0.010596047840089282 1.422288300683125E-4 36-37 4.7409610022770836E-5 0.0 0.0 0.01732821246332274 1.422288300683125E-4 38-39 4.7409610022770836E-5 0.0 0.0 0.02699977290796799 1.422288300683125E-4 40-41 4.7409610022770836E-5 0.0 0.0 0.039515909953979494 1.422288300683125E-4 42-43 4.7409610022770836E-5 0.0 0.0 0.05222168544008207 1.6593363507969792E-4 44-45 4.7409610022770836E-5 0.0 0.0 0.0690758018031771 1.8963844009108334E-4 46-47 4.7409610022770836E-5 0.0 0.0 0.0879448265922399 1.8963844009108334E-4 48-49 4.7409610022770836E-5 0.0 0.0 0.1079516820218492 1.8963844009108334E-4 50-51 4.7409610022770836E-5 0.0 0.0 0.13229651676854204 1.8963844009108334E-4 52-53 4.7409610022770836E-5 0.0 0.0 0.1578502965708155 2.3704805011385419E-4 54-55 4.7409610022770836E-5 2.3704805011385418E-5 0.0 0.188879886330719 2.3704805011385419E-4 56-57 9.481922004554167E-5 4.7409610022770836E-5 0.0 0.2245082082628313 2.84457660136625E-4 58-59 9.481922004554167E-5 4.7409610022770836E-5 0.0 0.26812504948378046 2.84457660136625E-4 60-61 9.481922004554167E-5 4.7409610022770836E-5 0.0 0.3158902315817221 2.84457660136625E-4 62-63 9.481922004554167E-5 7.111441503415625E-5 0.0 0.36232794459902606 2.84457660136625E-4 64-65 9.481922004554167E-5 1.6593363507969792E-4 0.0 0.4148340876992448 3.0816246514801045E-4 66-67 9.481922004554167E-5 1.8963844009108334E-4 0.0 0.4820135051015111 3.3186727015939584E-4 68-69 9.481922004554167E-5 2.3704805011385419E-4 0.0 0.5577977667229103 3.3186727015939584E-4 70-71 9.481922004554167E-5 2.3704805011385419E-4 0.0 0.6350043166449926 3.3186727015939584E-4 72-73 9.481922004554167E-5 2.3704805011385419E-4 0.0 0.7252485093233368 3.5557207517078124E-4 74-75 9.481922004554167E-5 2.3704805011385419E-4 0.0 0.8228886011652334 3.792768801821667E-4 76-77 9.481922004554167E-5 2.3704805011385419E-4 0.0 0.9317647705825267 3.792768801821667E-4 78-79 9.481922004554167E-5 2.3704805011385419E-4 0.0 1.0606003858194062 3.792768801821667E-4 80-81 9.481922004554167E-5 2.607528551252396E-4 0.0 1.202947739912776 4.0298168519355213E-4 82-83 9.481922004554167E-5 2.84457660136625E-4 0.0 1.3490167483929327 4.2668649020493753E-4 84-85 9.481922004554167E-5 2.84457660136625E-4 0.0 1.5040461731673935 4.2668649020493753E-4 86-87 9.481922004554167E-5 2.84457660136625E-4 0.0 1.6737014626338786 4.2668649020493753E-4 88-89 9.481922004554167E-5 2.84457660136625E-4 0.0 1.8601397540484252 4.2668649020493753E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5330 0.0 48.25219 1 GTATCAA 8700 0.0 38.46807 1 ATCAACG 10930 0.0 30.563747 3 TATCAAC 11115 0.0 30.102085 2 TCAACGC 11100 0.0 30.096373 4 CAACGCA 11195 0.0 29.798527 5 AACGCAG 11660 0.0 28.602003 6 GTACATG 8455 0.0 26.313538 1 TACATGG 8555 0.0 25.221401 2 ACGCAGA 13310 0.0 24.877838 7 CGCAGAG 13400 0.0 24.675295 8 ACATGGG 8470 0.0 24.236603 3 GCAGAGT 14390 0.0 22.713037 9 GAGTACT 8585 0.0 22.131222 12-13 GTACTTT 8835 0.0 21.450455 14-15 TAACGCA 210 8.578918E-7 20.365965 4 ATAACGC 235 1.12810085E-7 20.221045 3 AGTACTT 8905 0.0 20.055782 12-13 CAGAGTA 14110 0.0 19.91359 10-11 TATAACG 240 1.4063698E-7 19.802597 2 >>END_MODULE