FastQCFastQC Report
Thu 26 May 2016
SRR937525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109277
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104790.49680530342861556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91420.43341865482817094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62160.2946981359015435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.1145890274250371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62650.044.292831
GTATCAA98950.035.679171
TATCAAC127350.027.4887982
ATCAACG127200.027.4838713
TCAACGC128050.027.3756224
CAACGCA130650.026.7944775
AACGCAG135150.025.8659486
ACGCAGA156350.022.2398227
CGCAGAG157050.022.08028
TAGACCG1302.942929E-421.9227545
GTACATG84600.021.79231
TACATGG83950.021.6137372
GAGTACT99500.021.48362412-13
TATTCCG1604.9923863E-520.7809455
AGTACTT103550.020.4598712-13
GCAGAGT166550.020.3644039
GTACTTT104800.020.0550514-15
GGACCGA4500.020.054316
AGAGTAC147250.019.9366110-11
ACATGGG85750.019.8860053
Produced by FastQC (version 0.11.5)