##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937525_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2109277 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71315858467143 31.0 31.0 34.0 30.0 34.0 2 31.953174002276608 31.0 31.0 34.0 30.0 34.0 3 32.010121477643764 31.0 31.0 34.0 30.0 34.0 4 35.75004468355745 37.0 35.0 37.0 35.0 37.0 5 35.63538880858228 37.0 35.0 37.0 33.0 37.0 6 35.691240647861804 37.0 35.0 37.0 33.0 37.0 7 35.634993886530786 37.0 35.0 37.0 33.0 37.0 8 35.586219827931565 37.0 35.0 37.0 33.0 37.0 9 37.18933501858694 39.0 37.0 39.0 33.0 39.0 10-11 37.07208394155913 39.0 37.0 39.0 33.0 39.0 12-13 36.93830824495787 39.0 37.0 39.0 33.0 39.0 14-15 37.97612286105618 40.0 37.0 41.0 33.0 41.0 16-17 37.909379612066125 40.0 37.0 41.0 33.0 41.0 18-19 37.86241707466587 40.0 37.0 41.0 33.0 41.0 20-21 37.72981310657633 39.0 37.0 41.0 32.0 41.0 22-23 37.66768518312199 39.0 37.0 40.5 32.0 41.0 24-25 37.6888466996037 39.0 37.0 41.0 32.0 41.0 26-27 37.450842160607635 39.0 36.0 41.0 32.0 41.0 28-29 37.388682235666536 39.0 36.0 40.5 32.0 41.0 30-31 37.3783083018494 39.0 36.0 40.5 32.0 41.0 32-33 37.220195356039056 39.0 36.0 40.0 31.5 41.0 34-35 37.02465347130794 39.0 36.0 40.0 31.0 41.0 36-37 36.79405265406108 39.0 35.0 40.0 30.5 41.0 38-39 36.60946144105303 39.0 35.0 40.0 30.0 41.0 40-41 36.38240496625147 38.5 35.0 40.0 30.0 41.0 42-43 36.10639688386115 38.0 35.0 40.0 30.0 41.0 44-45 35.73260956242352 38.0 35.0 40.0 29.0 41.0 46-47 35.344579682990904 38.0 34.5 40.0 27.5 41.0 48-49 34.939710621222346 38.0 34.0 40.0 26.0 41.0 50-51 34.631445514268634 38.0 34.0 40.0 25.0 41.0 52-53 34.29362999738773 38.0 33.0 40.0 24.0 41.0 54-55 34.003635605944595 38.0 33.0 40.0 23.5 41.0 56-57 33.6514772597435 37.0 33.0 40.0 23.0 41.0 58-59 33.33748293846659 37.0 32.0 39.0 22.0 40.5 60-61 32.90066619984004 36.0 31.5 39.0 21.0 40.0 62-63 32.38720874498703 36.0 31.0 39.0 19.0 40.0 64-65 31.909290244951233 35.0 30.5 38.5 18.0 40.0 66-67 31.403435632209522 35.0 30.0 38.0 14.5 40.0 68-69 30.83641954091378 34.0 29.0 38.0 11.5 39.5 70-71 30.342403107794755 34.0 29.0 37.0 8.0 39.0 72-73 29.778107142874077 34.0 28.0 36.0 7.5 39.0 74-75 29.211102903980844 33.5 27.5 36.0 7.0 38.5 76-77 28.16454500760213 31.5 26.5 34.5 2.0 37.0 78-79 28.776589324209198 33.0 28.0 35.0 2.0 37.0 80-81 28.55531563659017 33.0 27.5 35.0 2.0 37.0 82-83 28.04982489260538 33.0 26.5 35.0 2.0 36.0 84-85 27.493072033687373 32.5 26.0 35.0 2.0 36.0 86-87 26.971737472129078 32.0 25.5 34.5 2.0 35.5 88-89 26.364987149625204 32.0 24.5 34.0 2.0 35.0 90-91 25.624780434243583 31.0 22.5 34.0 2.0 35.0 92-93 24.888173293502938 31.0 19.5 34.0 2.0 35.0 94-95 24.084119582207553 31.0 15.5 34.0 2.0 35.0 96-97 23.22419506778863 30.0 4.5 34.0 2.0 35.0 98-99 21.988566461398857 29.5 2.0 34.0 2.0 35.0 100-101 19.43447897075633 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20107283157796374 1101 2 0.19878351319355048 1101 3 0.1927758199614722 1101 4 0.18436528109691608 1101 5 0.1642205933055365 1101 6 0.12663384406334188 1101 7 0.09448215593438647 1101 8 0.05197800889340698 1101 9 0.10804857671774215 1101 10-11 0.12798143329760592 1101 12-13 0.2086852022313579 1101 14-15 0.2862235734660672 1101 16-17 0.24257077109466962 1101 18-19 0.23318634261942606 1101 20-21 0.33416946217426613 1101 22-23 0.20737750952170586 1101 24-25 0.19267615710671748 1101 26-27 0.19492250955769208 1101 28-29 0.2335615883449904 1101 30-31 0.38592004664494794 1101 32-33 0.1257230793008901 1101 34-35 0.14042455167166423 1101 36-37 -0.0013506605961808305 1101 38-39 0.11349147193378428 1101 40-41 0.019627404638363544 1101 42-43 0.172232052615108 1101 44-45 0.20999317212897495 1101 46-47 0.34571562213260165 1101 48-49 0.12430480469362237 1101 50-51 0.1713583975576256 1101 52-53 0.09203702620578014 1101 54-55 0.2110006383067784 1101 56-57 0.3761335286431944 1101 58-59 0.12673866200967154 1101 60-61 0.09744617990423166 1101 62-63 0.07536785736193963 1101 64-65 0.4065861709875769 1101 66-67 0.1426888773252024 1101 68-69 0.2509399912377823 1101 70-71 0.0575972816399819 1101 72-73 0.027243969578027105 1101 74-75 0.23973109058460906 1101 76-77 0.13551822756172882 1101 78-79 0.29961457914502887 1101 80-81 0.3879705844332513 1101 82-83 0.5780624456881434 1101 84-85 0.6649606608295429 1101 86-87 0.5405335627413201 1101 88-89 0.5947717116201545 1101 90-91 0.5733297669640294 1101 92-93 0.7922701692567067 1101 94-95 1.008951185258173 1101 96-97 0.9818991375733042 1101 98-99 1.4938183076781577 1101 100-101 1.6867644170703926 1102 1 0.11902210440141658 1102 2 0.18476449737222467 1102 3 0.1363219134055491 1102 4 0.16083595015430063 1102 5 -0.023386490912294278 1102 6 0.06744297825266443 1102 7 0.059335518395997155 1102 8 0.10205426453529753 1102 9 0.22337973916973652 1102 10-11 0.07583150508782666 1102 12-13 0.1748711492096433 1102 14-15 0.12263049005887439 1102 16-17 0.27199308308328796 1102 18-19 0.2443177771185816 1102 20-21 0.2142912994543451 1102 22-23 0.24723980282426794 1102 24-25 0.24862890608825694 1102 26-27 0.23185136510476667 1102 28-29 0.22335312190777756 1102 30-31 0.28619758970190645 1102 32-33 0.235016649782402 1102 34-35 0.34709193967548657 1102 36-37 0.3081029780345119 1102 38-39 0.3208925628703554 1102 40-41 0.4061165880160118 1102 42-43 0.32558352242542554 1102 44-45 0.24529180571030906 1102 46-47 0.30355655698554784 1102 48-49 0.523558735981112 1102 50-51 0.3804539438159438 1102 52-53 0.5531141886715076 1102 54-55 0.48698114159401484 1102 56-57 0.5266248966419269 1102 58-59 0.5646282699457501 1102 60-61 0.5405129276354543 1102 62-63 0.5036260685831948 1102 64-65 0.6654356038791711 1102 66-67 0.5117704777319005 1102 68-69 0.46869278556051697 1102 70-71 0.3824863331159776 1102 72-73 0.43486936201978565 1102 74-75 0.6229612040062911 1102 76-77 0.41946504096897996 1102 78-79 0.4426449165247206 1102 80-81 0.6373512649633319 1102 82-83 0.6715910209442164 1102 84-85 0.7653409085573344 1102 86-87 0.778126220216091 1102 88-89 1.0297483196867638 1102 90-91 0.8427816537759227 1102 92-93 1.2218941121542848 1102 94-95 1.4880002911435959 1102 96-97 1.5283788405197924 1102 98-99 1.4170529208696934 1102 100-101 1.6831604792565251 1103 1 0.10481708506712195 1103 2 0.08998475667922534 1103 3 0.15878760451177598 1103 4 0.044413173598073286 1103 5 0.05174809093621491 1103 6 0.12722764729504377 1103 7 0.06977224597201115 1103 8 0.06825716088831513 1103 9 0.16277241488434413 1103 10-11 0.140093389991911 1103 12-13 0.23143154044475978 1103 14-15 0.29349636313492766 1103 16-17 0.26072232715031163 1103 18-19 0.19590811755379178 1103 20-21 0.25028530961432693 1103 22-23 0.23797640150498012 1103 24-25 0.15198018366805854 1103 26-27 0.13004872654233424 1103 28-29 0.2955667089879199 1103 30-31 0.1957206771232407 1103 32-33 0.08593028993707463 1103 34-35 0.24866711595875302 1103 36-37 0.3252005593522753 1103 38-39 0.26444211173683385 1103 40-41 0.350098315212918 1103 42-43 0.4046911120834764 1103 44-45 0.3704131189258675 1103 46-47 0.2794758836972093 1103 48-49 0.20423581536544333 1103 50-51 0.45108287701786054 1103 52-53 0.197736601288085 1103 54-55 0.31671425251979457 1103 56-57 0.28080072498372743 1103 58-59 0.2134018691572095 1103 60-61 0.390643718445979 1103 62-63 0.40260248832692724 1103 64-65 0.41348701035427027 1103 66-67 0.5519460027906646 1103 68-69 0.6699589922479063 1103 70-71 0.6717444269820732 1103 72-73 0.7021925100977278 1103 74-75 0.5755068860140256 1103 76-77 0.44157058346651823 1103 78-79 0.6487428011255219 1103 80-81 0.8031674754417608 1103 82-83 0.8671213871584875 1103 84-85 0.7718842401215156 1103 86-87 0.8421303145371155 1103 88-89 0.9295701409014114 1103 90-91 1.0762015491432777 1103 92-93 1.3674661797864012 1103 94-95 1.4067412874283782 1103 96-97 1.2688690217314083 1103 98-99 1.3333922945902188 1103 100-101 1.6808896475027915 1104 1 0.2163666811795153 1104 2 0.1922600288992946 1104 3 0.24600036032363093 1104 4 0.1370972983058536 1104 5 0.16714654039355992 1104 6 0.2529775137969281 1104 7 0.2602177099828822 1104 8 0.24285894360160398 1104 9 0.2514196280330623 1104 10-11 0.36040421289393976 1104 12-13 0.21030436987257417 1104 14-15 0.27175626208712345 1104 16-17 0.3552553337778974 1104 18-19 0.282173706472463 1104 20-21 0.2755266331298145 1104 22-23 0.22987248550074924 1104 24-25 0.16091410969134756 1104 26-27 0.3162404673398882 1104 28-29 0.2950641488601846 1104 30-31 0.3492517245878517 1104 32-33 0.2839114769719515 1104 34-35 0.2853743648617453 1104 36-37 0.33199824764712815 1104 38-39 0.3854965057446904 1104 40-41 0.43776532180120853 1104 42-43 0.4661032810394019 1104 44-45 0.456153734320921 1104 46-47 0.4186361046522151 1104 48-49 0.18706679692930805 1104 50-51 0.31663839883921696 1104 52-53 0.5352221575957188 1104 54-55 0.4454004524758517 1104 56-57 0.496279541199236 1104 58-59 0.4494155529372392 1104 60-61 0.41450862728328275 1104 62-63 0.3085484675780137 1104 64-65 0.5959557164316003 1104 66-67 0.6168216218379428 1104 68-69 0.599860605476632 1104 70-71 0.70799688933025 1104 72-73 0.5871951800600286 1104 74-75 0.8963967960568304 1104 76-77 0.7605643737950025 1104 78-79 0.8922737235751725 1104 80-81 0.8969155667312556 1104 82-83 0.7649520204663993 1104 84-85 0.6384132712734569 1104 86-87 0.8876834432150105 1104 88-89 1.0792262604728577 1104 90-91 1.1415548146232517 1104 92-93 1.4887221157644568 1104 94-95 1.442593969802875 1104 96-97 1.9145500228511843 1104 98-99 2.0624579149412234 1104 100-101 1.9932565735457253 1105 1 0.15129654958885297 1105 2 0.13407622315609657 1105 3 0.06340991866643364 1105 4 0.08566886909458304 1105 5 0.06404003590749596 1105 6 0.1698639449398911 1105 7 0.1751153962578087 1105 8 0.14156907369898875 1105 9 0.23458479168620272 1105 10-11 0.2587936703253604 1105 12-13 0.31891802057293006 1105 14-15 0.26498336821254753 1105 16-17 0.3557600821264302 1105 18-19 0.3236284499869768 1105 20-21 0.3687269367637569 1105 22-23 0.3259553013704988 1105 24-25 0.4137293763904992 1105 26-27 0.4676997194163661 1105 28-29 0.49933713606487373 1105 30-31 0.3643187451750336 1105 32-33 0.20325406928977685 1105 34-35 0.2787849723306479 1105 36-37 0.23567440080039148 1105 38-39 0.41400880473796775 1105 40-41 0.4790486250934194 1105 42-43 0.6100574526078972 1105 44-45 0.6269918578019116 1105 46-47 0.5376887514461117 1105 48-49 0.5528643289941826 1105 50-51 0.564809559549829 1105 52-53 0.6661050412374792 1105 54-55 0.5689415593613134 1105 56-57 0.6766329320494435 1105 58-59 0.5463187164927419 1105 60-61 0.6874283471206653 1105 62-63 0.64858315765985 1105 64-65 0.4865005632218633 1105 66-67 0.47387244797688055 1105 68-69 0.5086500039425736 1105 70-71 0.4843127720080851 1105 72-73 0.543029364532007 1105 74-75 0.7115220163398099 1105 76-77 0.8605623636081425 1105 78-79 0.9678869023602346 1105 80-81 1.099359858231324 1105 82-83 1.0648809938639694 1105 84-85 1.1520283626077301 1105 86-87 1.3672100729261452 1105 88-89 1.6397862985323997 1105 90-91 1.6333612929768897 1105 92-93 1.6880617215189524 1105 94-95 1.818948524646995 1105 96-97 1.866062592741077 1105 98-99 2.1029730396828903 1105 100-101 2.5978998180256134 1106 1 -0.21539573840692228 1106 2 -0.1927743438464553 1106 3 -0.22114289017074285 1106 4 -0.2644325790172388 1106 5 -0.047757873563071485 1106 6 -0.07115144828495801 1106 7 -0.18376484081391453 1106 8 -0.15527484727647334 1106 9 -0.21771203061974376 1106 10-11 -0.38734113523678104 1106 12-13 -0.4996067229327039 1106 14-15 -0.2934364687768962 1106 16-17 -0.2415967325691284 1106 18-19 -0.38251691605994864 1106 20-21 -0.2669729174416915 1106 22-23 -0.28736884280505137 1106 24-25 -0.3659454433377931 1106 26-27 -0.3865919561377993 1106 28-29 -0.44227141151455385 1106 30-31 -0.5104268031982073 1106 32-33 -0.6382055246232383 1106 34-35 -0.7749307458797077 1106 36-37 -0.7467950994459258 1106 38-39 -0.6036094615278174 1106 40-41 -0.8260232579365052 1106 42-43 -0.9681175692565276 1106 44-45 -0.9503412468713464 1106 46-47 -0.7829822930157633 1106 48-49 -0.690820638870683 1106 50-51 -0.5325235023684343 1106 52-53 -0.6657331339411527 1106 54-55 -0.7420643612559488 1106 56-57 -0.9010068209786795 1106 58-59 -0.9554328133048813 1106 60-61 -0.8754580808749246 1106 62-63 -0.9054152186002966 1106 64-65 -1.2955084419310658 1106 66-67 -1.0492040877470608 1106 68-69 -0.8820857953407568 1106 70-71 -0.8826375899129815 1106 72-73 -0.8150821139026085 1106 74-75 -0.6983834618582314 1106 76-77 -0.7359815122845497 1106 78-79 -0.7122235195508466 1106 80-81 -0.8153713529655455 1106 82-83 -0.9578308179646271 1106 84-85 -0.667595303139386 1106 86-87 -0.43117195914295436 1106 88-89 -0.5987965727698281 1106 90-91 -0.7713471963132221 1106 92-93 -0.42746299870896465 1106 94-95 -0.12853895407645055 1106 96-97 -0.18676572170959105 1106 98-99 -0.21125791427139973 1106 100-101 0.03134774267419971 1107 1 0.19818961095251453 1107 2 0.1694147694947823 1107 3 0.07137533005541385 1107 4 0.12209376107951186 1107 5 0.1357245754049785 1107 6 0.09082536083681703 1107 7 0.045457268043250565 1107 8 0.09281217624664606 1107 9 0.18390181133260342 1107 10-11 0.14423505275966164 1107 12-13 0.2280811875083728 1107 14-15 0.24725254206452263 1107 16-17 0.26802210948925875 1107 18-19 0.41564916192003665 1107 20-21 0.2623410878034633 1107 22-23 0.21814788587204248 1107 24-25 0.16249707782782252 1107 26-27 0.2676959528894898 1107 28-29 0.27413421442944497 1107 30-31 0.2943007947009022 1107 32-33 0.39695980640404827 1107 34-35 0.388812358945799 1107 36-37 0.35898989599459696 1107 38-39 0.4320848628098801 1107 40-41 0.47374912177937034 1107 42-43 0.4906611277469395 1107 44-45 0.7457604612233126 1107 46-47 0.5398798788964569 1107 48-49 0.48541118508411074 1107 50-51 0.5514124199296333 1107 52-53 0.5461448276555885 1107 54-55 0.6701297003523266 1107 56-57 0.63664807745225 1107 58-59 0.7813436499094664 1107 60-61 0.8150593900422223 1107 62-63 0.7705178242776114 1107 64-65 0.8878101251450481 1107 66-67 1.1263189558613078 1107 68-69 1.202516611402082 1107 70-71 0.9921567624648944 1107 72-73 0.9898834720782439 1107 74-75 1.157787851859549 1107 76-77 0.9098500439925665 1107 78-79 1.1481528786291335 1107 80-81 1.3745816620606917 1107 82-83 1.311552722214124 1107 84-85 1.3667519144029256 1107 86-87 1.5834981210425347 1107 88-89 1.654491315050599 1107 90-91 1.7053345664951323 1107 92-93 1.5889068112068934 1107 94-95 1.786140787011366 1107 96-97 1.813286117278512 1107 98-99 1.8533560855542355 1107 100-101 2.3551822490609986 1108 1 0.1329351916009962 1108 2 0.06977319386644254 1108 3 0.04359829059876219 1108 4 0.1063366782761932 1108 5 0.053382696310002586 1108 6 0.11048224469313084 1108 7 0.05721502632712827 1108 8 0.02257259048809601 1108 9 0.06166461173908999 1108 10-11 0.05074035018594003 1108 12-13 0.1361046174907159 1108 14-15 0.09395272962029111 1108 16-17 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80-81 0.31777468311044643 2316 82-83 0.16758568483437486 2316 84-85 -0.04447015844772295 2316 86-87 0.32060143767341387 2316 88-89 0.7360225870562331 2316 90-91 0.6511941610968037 2316 92-93 0.8941951468389995 2316 94-95 0.585639713281946 2316 96-97 0.9416133690549664 2316 98-99 0.9515944709445314 2316 100-101 1.0735468049327572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 28.0 5 83.0 6 162.0 7 388.0 8 899.0 9 1722.0 10 2766.0 11 4447.0 12 6463.0 13 9166.0 14 11938.0 15 15830.0 16 20317.0 17 25772.0 18 23960.0 19 18157.0 20 16991.0 21 18046.0 22 19579.0 23 22050.0 24 25329.0 25 30077.0 26 36447.0 27 44056.0 28 54134.0 29 65358.0 30 79570.0 31 95881.0 32 118500.0 33 148525.0 34 189815.0 35 246465.0 36 310025.0 37 314953.0 38 126996.0 39 4365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34775488795188 20.959374524270334 15.837215445486894 21.855655142290892 2 16.52727451159805 21.563360336266882 38.83420717146207 23.075157980672998 3 20.264716298523144 24.740799809603008 30.158627814175187 24.83585607769866 4 13.881499567386898 17.82844816346849 36.41189508243354 31.878157186711075 5 13.529549542886024 37.80163311210174 34.18350558673786 14.485311758274374 6 31.924730606743445 37.03591325368835 16.677041469659983 14.36231466990822 7 28.27651370588121 33.07631003419655 20.32919336815411 18.31798289176813 8 26.311859466537584 36.00423272998283 19.046004863277798 18.637902940201784 9 25.9298328289741 17.01886475792416 20.363849793080757 36.687452620020984 10-11 25.05232835706263 27.36333350242761 26.660272690594926 20.924065449914828 12-13 25.767218319174578 24.406261586267256 27.881430585754263 21.945089508803903 14-15 22.538670833655324 25.886026349313056 26.311006093557175 25.264296723474445 16-17 21.638551977762997 29.28228487770928 27.065577446679594 22.013585697848125 18-19 21.58334348689148 27.36283570152237 29.959460042469527 21.094360769116623 20-21 22.108665428483043 26.381347123237987 30.470150253227118 21.03983719505185 22-23 21.96023788618102 26.22792811073541 30.38519962602714 21.426634377056423 24-25 21.83693749090328 26.506404801266026 30.387521411365125 21.269136296465565 26-27 21.521082088225462 26.795930316628386 30.31897518318079 21.364012411965362 28-29 21.477571708732675 26.962568824998712 30.40749898838186 21.15236047788675 30-31 21.67859453229579 26.628751612223432 30.365791303321306 21.326862552159472 32-33 21.47795192381086 26.646879475763498 30.272979793550114 21.60218880687553 34-35 21.953138445069094 26.703652483765765 30.15400537719797 21.189203693967173 36-37 21.995048055668484 26.487900449088492 30.055362690216654 21.461688805026377 38-39 21.67384843242495 26.792735140998552 30.147107278939657 21.386309147636844 40-41 21.96525392972797 26.700721029908625 29.971798360052237 21.36222668031117 42-43 21.750747967583763 26.724687944770725 29.81815241871106 21.706411668934457 44-45 21.902670915199852 26.889403335834984 29.684745057192586 21.523180691772584 46-47 22.187555261826684 27.22385442974062 29.027268585396797 21.561321723035903 48-49 22.202370764958797 27.248886703832643 28.93403758728702 21.61470494392154 50-51 22.21661735277064 27.474627561956062 28.717849765583182 21.590905319690112 52-53 22.408673682972886 27.551762997463115 28.442874027451115 21.596689292112888 54-55 22.191395440238527 27.476192079086815 28.618977023880692 21.713435456793963 56-57 22.28367824614785 27.76019934792822 28.38920634890534 21.566916057018588 58-59 22.38153168123485 27.317891391220783 28.549213782732185 21.75136314481218 60-61 22.220481235987496 27.503334080824853 28.715858561962225 21.560326121225426 62-63 22.039447640115547 27.5756811457196 28.809397722537156 21.5754734916277 64-65 22.12163219909002 27.41704859058341 28.66614958585335 21.79516962447322 66-67 22.004886034408948 27.61130946765171 28.502728660104857 21.88107583783448 68-69 22.02821156254015 27.482379033194785 28.59897016845109 21.89043923581398 70-71 22.116393437182506 27.513977538274965 28.4667921757076 21.902836848834934 72-73 22.1594887726932 27.50354742406995 28.346964386375046 21.989999416861796 74-75 22.100795675485013 27.886427434613854 28.022279672134097 21.990497217767036 76-77 22.120849940524646 27.640750835475853 28.274759550310367 21.963639673689137 78-79 22.198103900056747 27.599030378655815 28.21625609154227 21.986609629745168 80-81 22.07799165306406 27.890741709125923 28.13404782776278 21.897218810047235 82-83 22.27025356153856 27.781802338343436 28.056233646601125 21.89171045351688 84-85 22.169384074074863 27.83522652252211 28.01690062184977 21.978488781553253 86-87 22.191703602703676 27.817707204885846 28.038778216422028 21.951810975988455 88-89 22.18165276537885 27.80419546602936 27.96799566865803 22.04615609993377 90-91 22.227184945688997 27.78009318856721 27.981628756358884 22.011093109384905 92-93 22.218360875848177 27.6298297383303 28.0749460465081 22.076863339313416 94-95 22.106721876737858 27.620838799266288 28.029011836757334 22.243427487238517 96-97 22.178523731117345 27.554939441334636 27.9739692795209 22.29256754802712 98-99 22.03387620729087 27.580682018596026 27.810429969069837 22.575011805043268 100-101 22.26434060651896 27.35430612339246 28.09844938543221 22.28290388465637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 641.0 1 464.5 2 415.5 3 574.5 4 484.0 5 405.0 6 795.0 7 1807.0 8 2848.5 9 3311.5 10 3065.0 11 2662.5 12 2658.0 13 2886.0 14 3223.0 15 3527.0 16 3750.5 17 3976.0 18 4138.0 19 4310.0 20 4778.0 21 5551.5 22 6477.0 23 7662.5 24 9234.0 25 11320.5 26 14123.0 27 17365.5 28 20070.0 29 23294.5 30 27423.5 31 31079.5 32 35495.5 33 39342.5 34 42974.0 35 47065.0 36 51769.0 37 56745.0 38 60202.5 39 62276.5 40 63892.5 41 67573.5 42 70610.0 43 73038.0 44 76469.5 45 78836.0 46 79458.0 47 80683.5 48 82248.0 49 80473.0 50 76906.0 51 72932.5 52 69382.0 53 67381.0 54 65808.5 55 60728.5 56 53633.0 57 47940.5 58 43086.0 59 37964.0 60 31530.0 61 25600.0 62 20615.5 63 15672.0 64 11657.0 65 8950.5 66 6680.5 67 5048.5 68 3772.5 69 2666.0 70 1987.0 71 1480.5 72 1124.5 73 948.5 74 798.0 75 624.5 76 445.0 77 280.0 78 160.5 79 105.0 80 80.5 81 56.0 82 35.0 83 21.0 84 9.0 85 4.5 86 2.0 87 1.5 88 2.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031954077155347545 2 0.0 3 0.0 4 9.481922004554167E-5 5 0.008296681753984896 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004693551392254313 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.1334324510246874E-4 22-23 0.0015645171307514375 24-25 0.0 26-27 0.0010430114205009584 28-29 0.001540812325740052 30-31 2.3704805011385418E-5 32-33 0.0 34-35 0.0 36-37 2.1334324510246874E-4 38-39 0.0 40-41 1.1852402505692709E-4 42-43 0.018750500764005865 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.84457660136625E-4 84-85 2.607528551252396E-4 86-87 0.0 88-89 0.0 90-91 0.010430114205009583 92-93 0.002512709331206854 94-95 0.0 96-97 0.0 98-99 4.266864902049375E-4 100-101 0.013013937951250595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2109277.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.92434225286037 #Duplication Level Percentage of deduplicated Percentage of total 1 78.32400471698908 45.368664558415254 2 12.669157600268196 14.677052417867243 3 3.8094986956106016 6.61988118769121 4 1.685744092709386 3.9058247110734428 5 0.8914839349026227 2.581931027911311 6 0.5536920414273881 1.9243348386194987 7 0.36864758023799565 1.4947568025876126 8 0.2590840194310823 1.2005817131018188 9 0.191850253124528 1.000151975095475 >10 1.1277905238854606 12.089191039328808 >50 0.08032622627006444 3.174185239951132 >100 0.03634281018935877 3.7258065051271236 >500 0.0018856073775745954 0.753018367998288 >1k 2.4594878837929084E-4 0.2576702821945297 >5k 1.639658589195272E-4 0.7293218197463301 >10k+ 8.19829294597636E-5 0.49762751329090993 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10479 0.49680530342861556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9142 0.43341865482817094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6216 0.2946981359015435 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 0.1145890274250371 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.7409610022770836E-5 2 0.0 0.0 0.0 0.0 4.7409610022770836E-5 3 0.0 0.0 0.0 0.0 4.7409610022770836E-5 4 0.0 0.0 0.0 0.0 4.7409610022770836E-5 5 0.0 0.0 0.0 0.0 4.7409610022770836E-5 6 0.0 0.0 0.0 0.0 4.7409610022770836E-5 7 0.0 0.0 0.0 0.0 4.7409610022770836E-5 8 0.0 0.0 0.0 0.0 4.7409610022770836E-5 9 0.0 0.0 0.0 0.0 4.7409610022770836E-5 10-11 0.0 0.0 0.0 0.0 4.7409610022770836E-5 12-13 0.0 0.0 0.0 0.0 1.1852402505692708E-4 14-15 0.0 0.0 0.0 2.3704805011385418E-5 1.422288300683125E-4 16-17 0.0 0.0 0.0 9.481922004554167E-5 1.422288300683125E-4 18-19 0.0 0.0 0.0 1.1852402505692708E-4 1.422288300683125E-4 20-21 0.0 0.0 0.0 1.8963844009108334E-4 1.422288300683125E-4 22-23 0.0 0.0 0.0 2.84457660136625E-4 1.422288300683125E-4 24-25 0.0 0.0 0.0 4.2668649020493753E-4 1.422288300683125E-4 26-27 0.0 0.0 0.0 4.503912952163229E-4 1.422288300683125E-4 28-29 0.0 0.0 0.0 0.0012326498605920418 1.422288300683125E-4 30-31 0.0 4.7409610022770836E-5 0.0 0.002773462186332094 1.422288300683125E-4 32-33 0.0 4.7409610022770836E-5 0.0 0.00564174359270973 1.422288300683125E-4 34-35 0.0 7.111441503415625E-5 0.0 0.0104064093999982 1.422288300683125E-4 36-37 0.0 9.481922004554167E-5 0.0 0.01718598363325443 1.422288300683125E-4 38-39 0.0 9.481922004554167E-5 0.0 0.026739020052842753 1.422288300683125E-4 40-41 0.0 9.481922004554167E-5 0.0 0.03932627151388841 1.422288300683125E-4 42-43 0.0 9.481922004554167E-5 0.0 0.05212686622003653 1.422288300683125E-4 44-45 0.0 9.481922004554167E-5 0.0 0.06905209699816572 1.6593363507969792E-4 46-47 0.0 9.481922004554167E-5 0.0 0.08792112178722852 1.8963844009108334E-4 48-49 0.0 1.1852402505692708E-4 0.0 0.10745388111661011 1.8963844009108334E-4 50-51 0.0 1.422288300683125E-4 0.0 0.13182242066831432 1.8963844009108334E-4 52-53 0.0 1.6593363507969792E-4 0.0 0.15692580917537147 1.8963844009108334E-4 54-55 0.0 1.8963844009108334E-4 0.0 0.18759982686010418 2.84457660136625E-4 56-57 0.0 2.3704805011385419E-4 0.0 0.22280146230201153 2.84457660136625E-4 58-59 0.0 2.84457660136625E-4 0.0 0.2651856536623687 2.84457660136625E-4 60-61 0.0 3.0816246514801045E-4 0.0 0.3118367099247752 2.84457660136625E-4 62-63 0.0 3.5557207517078124E-4 0.0 0.3574684595716921 2.84457660136625E-4 64-65 0.0 4.503912952163229E-4 0.0 0.4090264104714554 2.84457660136625E-4 66-67 0.0 4.7409610022770837E-4 0.0 0.47551838852839146 3.0816246514801045E-4 68-69 0.0 4.7409610022770837E-4 0.0 0.5498803618491075 3.3186727015939584E-4 70-71 2.3704805011385418E-5 4.7409610022770837E-4 0.0 0.6256646234705068 3.3186727015939584E-4 72-73 4.7409610022770836E-5 4.7409610022770837E-4 0.0 0.7140361365529515 3.3186727015939584E-4 74-75 9.481922004554167E-5 4.978009052390938E-4 0.0 0.8099457776290169 3.792768801821667E-4 76-77 9.481922004554167E-5 5.215057102504792E-4 0.0 0.9165225809602058 3.792768801821667E-4 78-79 9.481922004554167E-5 5.215057102504792E-4 0.0 1.0437225646512998 3.792768801821667E-4 80-81 1.422288300683125E-4 5.452105152618646E-4 0.0 1.1829408844831666 3.792768801821667E-4 82-83 1.422288300683125E-4 5.6891532027325E-4 0.0 1.3261890211669685 3.792768801821667E-4 84-85 1.422288300683125E-4 5.6891532027325E-4 0.0 1.4777338396047557 3.792768801821667E-4 86-87 1.422288300683125E-4 5.6891532027325E-4 0.0 1.6437622939044991 4.0298168519355213E-4 88-89 1.422288300683125E-4 5.6891532027325E-4 0.0 1.824582546531347 4.2668649020493753E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6265 0.0 44.29283 1 GTATCAA 9895 0.0 35.67917 1 TATCAAC 12735 0.0 27.488798 2 ATCAACG 12720 0.0 27.483871 3 TCAACGC 12805 0.0 27.375622 4 CAACGCA 13065 0.0 26.794477 5 AACGCAG 13515 0.0 25.865948 6 ACGCAGA 15635 0.0 22.239822 7 CGCAGAG 15705 0.0 22.0802 8 TAGACCG 130 2.942929E-4 21.922754 5 GTACATG 8460 0.0 21.7923 1 TACATGG 8395 0.0 21.613737 2 GAGTACT 9950 0.0 21.483624 12-13 TATTCCG 160 4.9923863E-5 20.780945 5 AGTACTT 10355 0.0 20.45987 12-13 GCAGAGT 16655 0.0 20.364403 9 GTACTTT 10480 0.0 20.05505 14-15 GGACCGA 450 0.0 20.05431 6 AGAGTAC 14725 0.0 19.93661 10-11 ACATGGG 8575 0.0 19.886005 3 >>END_MODULE