FastQCFastQC Report
Thu 26 May 2016
SRR937524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025107
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95660.47237010192547846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75070.37069646196472583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49590.2448759497646297No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28300.13974570232585243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.047.072661
GTATCAA86900.040.7717671
ATCAACG107900.032.2940673
TATCAAC109400.031.9389742
TCAACGC109350.031.865844
CAACGCA111200.031.2494245
AACGCAG114650.030.3295146
ACGCAGA130600.026.3707987
CGCAGAG130650.026.3607088
GCAGAGT139550.024.4071889
GAGTACT82650.023.04440312-13
GTACATG78700.022.9027441
TACATGG78350.022.6925622
GTACTTT87900.021.39679714-15
ACATGGG79350.021.3875733
AGTACTT87100.021.26720612-13
CGCGGAA1259.5278665E-920.8959982-83
AGAGTAC126250.020.82384710-11
CAGAGTA137400.020.30934110-11
TACTTTT100100.018.57548514-15
Produced by FastQC (version 0.11.5)