FastQCFastQC Report
Thu 26 May 2016
SRR937524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025107
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99030.48901119792682557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86650.42787862567261875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59800.29529303883696023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.11401866666798346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.039.872241
TAGATCG651.3279777E-436.54115
GTATCAA97550.035.3145521
ATCAACG122300.027.9660433
TATCAAC123650.027.8143832
TCAACGC123300.027.7777584
CAACGCA126050.027.1717365
AACGCAG129700.026.4038146
ACGCAGA150100.022.8175417
CGCAGAG149850.022.760518
GTACATG81600.021.8947831
TACATGG79850.021.8331382
GAGTACT91250.021.81199612-13
GCAGAGT158000.021.1054339
AGTACTT94550.020.69902612-13
CCCGTAG1150.003716830220.6592791
GTACTTT96300.020.54307614-15
ATACGTC3052.0918378E-1020.2473665
ACATGGG81700.020.059593
AGAGTAC136200.019.89723810-11
Produced by FastQC (version 0.11.5)