##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937524_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2025107 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66726103855253 31.0 31.0 34.0 30.0 34.0 2 31.925294317781727 31.0 31.0 34.0 30.0 34.0 3 32.025213976347914 33.0 31.0 34.0 30.0 34.0 4 35.789147931442635 37.0 35.0 37.0 35.0 37.0 5 35.56859168429125 37.0 35.0 37.0 33.0 37.0 6 35.695265978538416 37.0 35.0 37.0 33.0 37.0 7 35.64297244540659 37.0 35.0 37.0 33.0 37.0 8 35.635116070410106 37.0 35.0 37.0 33.0 37.0 9 37.21660978901362 39.0 37.0 39.0 33.0 39.0 10-11 37.12232736344302 39.0 37.0 39.0 33.0 39.0 12-13 37.01576163629872 39.0 37.0 39.0 33.0 39.0 14-15 38.061973515473504 40.0 37.0 41.0 33.0 41.0 16-17 38.00646434978498 40.0 37.0 41.0 33.0 41.0 18-19 37.971330650676734 40.0 37.0 41.0 33.0 41.0 20-21 37.86402323432787 39.5 37.0 41.0 33.0 41.0 22-23 37.68238196796515 39.0 37.0 40.5 32.0 41.0 24-25 37.60500185916102 39.0 36.5 41.0 32.0 41.0 26-27 37.49290901665937 39.0 36.5 41.0 32.0 41.0 28-29 37.34366208797856 39.0 36.0 40.0 32.0 41.0 30-31 37.405111433618075 39.0 36.0 40.0 32.0 41.0 32-33 37.30209465475158 39.0 36.0 40.0 32.0 41.0 34-35 37.12177134344012 39.0 36.0 40.0 31.0 41.0 36-37 36.90577732435867 39.0 35.5 40.0 31.0 41.0 38-39 36.727357122364396 39.0 35.0 40.0 30.5 41.0 40-41 36.49120737817804 39.0 35.0 40.0 30.0 41.0 42-43 36.24670424821997 38.0 35.0 40.0 30.0 41.0 44-45 35.88115961280071 38.0 35.0 40.0 29.5 41.0 46-47 35.4815688751261 38.0 35.0 40.0 27.5 41.0 48-49 35.10349230929526 38.0 34.0 40.0 26.5 41.0 50-51 34.79142361366584 38.0 34.0 40.0 26.0 41.0 52-53 34.50099056494299 38.0 33.5 40.0 24.5 41.0 54-55 34.22686825930679 38.0 33.0 40.0 24.0 41.0 56-57 33.86600288281063 37.0 33.0 40.0 23.5 41.0 58-59 33.54467023223958 37.0 32.5 39.5 23.0 40.5 60-61 33.11380238180007 36.5 32.0 39.0 22.0 40.0 62-63 32.65457133869964 36.0 31.0 39.0 20.5 40.0 64-65 32.16546261506182 35.0 31.0 38.5 19.0 40.0 66-67 31.642195943226703 35.0 30.5 38.0 17.5 40.0 68-69 31.092582021591944 34.5 29.5 38.0 13.5 40.0 70-71 30.606947188469547 34.0 29.0 37.0 11.5 39.0 72-73 30.089414781539936 34.0 29.0 36.5 8.0 39.0 74-75 29.499054370954227 34.0 28.0 36.0 7.0 38.5 76-77 28.466450414718828 32.0 27.0 35.0 7.0 37.0 78-79 29.034608788572655 33.0 28.0 35.0 4.0 37.0 80-81 28.77827245671463 33.0 28.0 35.0 2.0 37.0 82-83 28.32320736632682 33.0 27.0 35.0 2.0 36.0 84-85 27.775352857898373 33.0 26.0 35.0 2.0 36.0 86-87 27.178067628031506 32.0 26.0 34.5 2.0 35.5 88-89 26.547221455458896 32.0 25.0 34.0 2.0 35.0 90-91 25.784595085593008 31.0 23.5 34.0 2.0 35.0 92-93 25.071353513666192 31.0 20.5 34.0 2.0 35.0 94-95 24.303027444969572 31.0 17.5 34.0 2.0 35.0 96-97 23.384150072070266 30.5 4.5 34.0 2.0 35.0 98-99 22.161944035549727 30.0 2.0 34.0 2.0 35.0 100-101 19.634539804563413 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1677341704687585 1101 2 0.12091150532210193 1101 3 0.06781261889032919 1101 4 0.15682037454039488 1101 5 -0.06983875207342294 1101 6 0.08073960975308836 1101 7 0.022917717766205215 1101 8 0.1482348056650835 1101 9 0.20489005613788436 1101 10-11 0.07604781248192438 1101 12-13 0.10635329496555102 1101 14-15 0.15675075496767477 1101 16-17 0.008104766788548545 1101 18-19 0.15845297336083064 1101 20-21 0.3376936285645158 1101 22-23 0.31740588282007565 1101 24-25 0.14108198811857875 1101 26-27 0.17318629516498163 1101 28-29 0.2530808799984996 1101 30-31 0.307861663998132 1101 32-33 0.31101641051878204 1101 34-35 0.16732504925187897 1101 36-37 0.24174518907831555 1101 38-39 0.466432286793264 1101 40-41 0.2984046509306424 1101 42-43 0.1033124737671045 1101 44-45 0.34555794832564146 1101 46-47 0.12760512163754356 1101 48-49 0.20419979722102966 1101 50-51 0.40019931066637326 1101 52-53 0.2200787847454322 1101 54-55 0.26671718932884403 1101 56-57 0.38809976259367573 1101 58-59 0.37000026860462754 1101 60-61 0.47215657630072627 1101 62-63 0.4194814488246479 1101 64-65 0.31683809467883606 1101 66-67 0.5123075792469045 1101 68-69 0.3171677009598639 1101 70-71 0.3054172107014743 1101 72-73 0.29105701726964384 1101 74-75 0.26841013625212895 1101 76-77 0.03925148860401251 1101 78-79 0.2066909178730647 1101 80-81 0.30527679169017574 1101 82-83 0.334763479505753 1101 84-85 0.6816555782891065 1101 86-87 0.8642622946376548 1101 88-89 0.8051426941592901 1101 90-91 0.9050376775003315 1101 92-93 1.2311949457374567 1101 94-95 1.2624412454256415 1101 96-97 1.4813850029929725 1101 98-99 1.7477776035966919 1101 100-101 2.4069805428158375 1102 1 0.1997630143721203 1102 2 0.20308300840747862 1102 3 0.1454723395903983 1102 4 0.0754197511494894 1102 5 0.12133736885995461 1102 6 0.1364859141744077 1102 7 0.08344793439268727 1102 8 0.11002220633305626 1102 9 0.243105256027917 1102 10-11 0.18952557585343754 1102 12-13 0.211641158161342 1102 14-15 0.20592582081751942 1102 16-17 0.20315609065832518 1102 18-19 0.3573118856588664 1102 20-21 0.1812223257221106 1102 22-23 0.3209836933731438 1102 24-25 0.30343129506987765 1102 26-27 0.27000683437210427 1102 28-29 0.08283349263052742 1102 30-31 0.2848775608377139 1102 32-33 0.1958879429533127 1102 34-35 0.2682577208051171 1102 36-37 0.25441464329549035 1102 38-39 0.22793696965521093 1102 40-41 0.3010062305838872 1102 42-43 0.300921725066452 1102 44-45 0.2724793891833741 1102 46-47 0.22959631317607432 1102 48-49 0.21134743089898933 1102 50-51 0.3412587779977869 1102 52-53 0.28461256042130145 1102 54-55 0.3271483061199021 1102 56-57 0.5054887652053779 1102 58-59 0.3732374989360494 1102 60-61 0.6566777764210983 1102 62-63 0.5735838290782453 1102 64-65 0.5168179403542865 1102 66-67 0.5881731006773627 1102 68-69 0.4665601920343754 1102 70-71 0.5922110198873902 1102 72-73 0.4333123697752832 1102 74-75 0.3822950727056309 1102 76-77 0.3433938059983994 1102 78-79 0.5854147311827198 1102 80-81 0.6263521781516701 1102 82-83 0.5919780775523655 1102 84-85 0.8579711820086473 1102 86-87 0.9344959361990313 1102 88-89 0.8298408436765143 1102 90-91 1.049328709661804 1102 92-93 1.2453921349057637 1102 94-95 1.306267985106068 1102 96-97 1.4418745394620807 1102 98-99 1.498736652238268 1102 100-101 1.908746693209821 1103 1 0.11522477797194242 1103 2 0.19021139083845284 1103 3 0.2127802229571074 1103 4 0.055224148042974264 1103 5 -0.07391672236458646 1103 6 0.021083098649505416 1103 7 0.09471025479792416 1103 8 -0.009134593338423258 1103 9 0.12527791574886749 1103 10-11 0.1590913323942047 1103 12-13 0.16950634737288794 1103 14-15 0.21458662505765602 1103 16-17 0.1796793390873006 1103 18-19 0.1721849412371128 1103 20-21 0.3162724757416484 1103 22-23 0.18279074992134525 1103 24-25 0.2536505289215185 1103 26-27 0.21962122491450486 1103 28-29 0.012055326526478893 1103 30-31 0.09284666350965409 1103 32-33 0.121987681477016 1103 34-35 0.3342300733484578 1103 36-37 0.17289583988989676 1103 38-39 0.12486504638044238 1103 40-41 0.1485749536626031 1103 42-43 0.29105390195777403 1103 44-45 0.18257078813673644 1103 46-47 0.2842008312038047 1103 48-49 0.42459135597238884 1103 50-51 0.4771451296418192 1103 52-53 0.17097174774739443 1103 54-55 0.3479748780869656 1103 56-57 0.43473985235948476 1103 58-59 0.23631523238625363 1103 60-61 0.21341925415587326 1103 62-63 0.35323136227425067 1103 64-65 0.4458810891234819 1103 66-67 0.5781315058559677 1103 68-69 0.21495466651706963 1103 70-71 0.4383872694798647 1103 72-73 0.650910246761466 1103 74-75 0.696912392253509 1103 76-77 0.5514635840342486 1103 78-79 0.6895722447072927 1103 80-81 0.7967295010259505 1103 82-83 0.7228197140274588 1103 84-85 0.920367461730141 1103 86-87 0.9514149904099156 1103 88-89 1.1901934245498147 1103 90-91 1.4871600166090673 1103 92-93 1.6980250385289715 1103 94-95 1.502510523685416 1103 96-97 1.8592984791510645 1103 98-99 2.2258309831408205 1103 100-101 2.742151779817867 1104 1 0.10811134450687376 1104 2 0.2299549523249773 1104 3 0.12207843614987723 1104 4 0.08518906972287255 1104 5 -0.11615312209806206 1104 6 0.04115126599236163 1104 7 0.04531916628492638 1104 8 0.0859136951594266 1104 9 0.11653631021636102 1104 10-11 0.08697789045201887 1104 12-13 0.14343411892469504 1104 14-15 0.183712108465393 1104 16-17 0.04471257787248817 1104 18-19 0.15706769419859512 1104 20-21 0.2606184914107601 1104 22-23 0.30081401000671093 1104 24-25 0.23582597251505177 1104 26-27 0.18972072934210615 1104 28-29 0.10228211207627425 1104 30-31 0.2166832495137072 1104 32-33 0.3722856522241713 1104 34-35 0.31494462669751044 1104 36-37 0.24454304026470197 1104 38-39 0.05904162703824767 1104 40-41 0.21910091371050555 1104 42-43 0.2544505480702526 1104 44-45 0.17057114304547127 1104 46-47 0.35853753899580454 1104 48-49 0.3614060161896617 1104 50-51 0.249656544083102 1104 52-53 0.49387991963399 1104 54-55 0.29886494736764035 1104 56-57 0.6623595506983619 1104 58-59 0.5376036415078573 1104 60-61 0.558749124303354 1104 62-63 0.48886979391673435 1104 64-65 0.566141461020365 1104 66-67 0.7506168672836004 1104 68-69 0.667116586279441 1104 70-71 0.7835925259720682 1104 72-73 0.7115046962827627 1104 74-75 0.7591429258203561 1104 76-77 0.5034522879900649 1104 78-79 0.7340726004637368 1104 80-81 0.871661020029677 1104 82-83 0.8293889729407269 1104 84-85 0.908243485554241 1104 86-87 1.1258082688454252 1104 88-89 1.42266062233627 1104 90-91 1.3290094640986787 1104 92-93 1.6126621415242752 1104 94-95 1.760553473293463 1104 96-97 2.0075898592204524 1104 98-99 2.3890581998934195 1104 100-101 2.7921656540366264 1105 1 0.15609465863105143 1105 2 0.15690777341279372 1105 3 0.04811618849529253 1105 4 0.014665960348033025 1105 5 -0.036807190471641604 1105 6 0.003690356283669871 1105 7 0.06005616426476479 1105 8 0.1162702687182886 1105 9 -0.014538081308216988 1105 10-11 0.08596771127222524 1105 12-13 -0.04105246292237297 1105 14-15 0.17303502651662228 1105 16-17 0.10636324215883519 1105 18-19 0.1608212089033998 1105 20-21 0.2144010216910246 1105 22-23 0.27523540674385316 1105 24-25 0.11594999810074569 1105 26-27 0.05151187351673059 1105 28-29 -0.05853949638497369 1105 30-31 0.08044660608116061 1105 32-33 0.23961062883547157 1105 34-35 0.07448220487071211 1105 36-37 0.22840262181422588 1105 38-39 0.0032090434626681485 1105 40-41 0.00788800358120767 1105 42-43 0.003149376242710389 1105 44-45 0.168742187056786 1105 46-47 0.20369006719073468 1105 48-49 0.261855614845274 1105 50-51 0.3549488196387145 1105 52-53 0.24476500791242728 1105 54-55 0.04453166267256137 1105 56-57 0.32699877002874445 1105 58-59 0.3112633473901454 1105 60-61 0.37883256943575816 1105 62-63 0.399327353887017 1105 64-65 0.5274330883284364 1105 66-67 0.6096887981456014 1105 68-69 0.2990399812218918 1105 70-71 0.46751651875155176 1105 72-73 0.5579320587876317 1105 74-75 0.5856184618602214 1105 76-77 0.5627610300570574 1105 78-79 0.8151976683259221 1105 80-81 0.8447390556821119 1105 82-83 0.8177892409828118 1105 84-85 0.9613184571934106 1105 86-87 0.9697576401614967 1105 88-89 1.110424674411103 1105 90-91 1.2479633595602984 1105 92-93 1.5882982147274127 1105 94-95 1.5896335190646198 1105 96-97 1.8909286372817213 1105 98-99 2.2334267069568945 1105 100-101 2.832409479390236 1106 1 -0.294407395952863 1106 2 -0.09809868547896272 1106 3 -0.2033639961819489 1106 4 0.0035152181927742276 1106 5 -0.12839218625821758 1106 6 -0.14734598930682097 1106 7 -0.24481956564487461 1106 8 -0.1615194797405337 1106 9 -0.0962955583116667 1106 10-11 -0.05083606905243698 1106 12-13 -0.24601543693758998 1106 14-15 -0.16227737343702842 1106 16-17 -0.05175699136160006 1106 18-19 0.13076546679985057 1106 20-21 -0.027895422974495432 1106 22-23 0.05039232056487464 1106 24-25 0.0389099073871364 1106 26-27 -0.21704749499971143 1106 28-29 -0.14033612138452867 1106 30-31 -0.19051946727352487 1106 32-33 -0.09508257855457458 1106 34-35 -0.4501302774520184 1106 36-37 -0.1985035321030395 1106 38-39 -0.1364724838958793 1106 40-41 -0.142153391995258 1106 42-43 -0.06509349497051176 1106 44-45 -0.15284213973168903 1106 46-47 -0.049147845965720194 1106 48-49 -0.06588486622978706 1106 50-51 0.01827246551375339 1106 52-53 0.025642805173923477 1106 54-55 -0.10112557942181155 1106 56-57 -0.06584482855635798 1106 58-59 -0.02215855256867627 1106 60-61 -0.06715480317233613 1106 62-63 0.11581830479079258 1106 64-65 0.19142203909672162 1106 66-67 0.39510174830271083 1106 68-69 -0.10301363190600554 1106 70-71 0.07367427694524764 1106 72-73 0.24130401603337504 1106 74-75 0.27995797818514845 1106 76-77 0.241353449612145 1106 78-79 0.47378672811767686 1106 80-81 0.48333003172079003 1106 82-83 0.47165827647960157 1106 84-85 0.6806870496108566 1106 86-87 0.6916439446729683 1106 88-89 0.6957922086719357 1106 90-91 0.9389759293381701 1106 92-93 1.1740998591120828 1106 94-95 0.9623841712322481 1106 96-97 1.5695883787276763 1106 98-99 1.8795906570762284 1106 100-101 2.2202691476473717 1107 1 0.11173677052302367 1107 2 0.18435692858243513 1107 3 0.14276738145969858 1107 4 0.08092318989945113 1107 5 -0.13123809659428076 1107 6 0.01628793426016273 1107 7 0.05908892314121772 1107 8 -0.038951923907802666 1107 9 0.21868869247579426 1107 10-11 0.13856301362501 1107 12-13 0.062422430642712357 1107 14-15 0.07087215329716656 1107 16-17 0.10617327002006505 1107 18-19 0.11297359323562972 1107 20-21 0.10471373604124068 1107 22-23 0.09400461363443213 1107 24-25 0.13813389355625105 1107 26-27 -0.12293509535653868 1107 28-29 -0.1829142556357155 1107 30-31 -0.10611118768751027 1107 32-33 0.036299010260727016 1107 34-35 0.0998093308307304 1107 36-37 0.19314662223135315 1107 38-39 0.03302890945550985 1107 40-41 0.1405252372311736 1107 42-43 0.22637351062657984 1107 44-45 -0.057967936073453075 1107 46-47 0.04324556067973617 1107 48-49 0.1702191180446917 1107 50-51 0.17419164323907665 1107 52-53 0.14218500437974768 1107 54-55 0.10617984478066234 1107 56-57 0.17692987481373734 1107 58-59 0.05371791676575555 1107 60-61 0.2719802137610259 1107 62-63 0.17554492372300246 1107 64-65 0.38222828825794863 1107 66-67 0.5009885592253163 1107 68-69 0.5255477031851363 1107 70-71 0.5735065221750908 1107 72-73 0.4970155725608123 1107 74-75 0.4946011933928105 1107 76-77 0.5493591019611088 1107 78-79 0.6720057645342798 1107 80-81 0.6216724567048075 1107 82-83 0.5579394887347746 1107 84-85 0.8315834521591476 1107 86-87 0.9522795971008762 1107 88-89 0.9813364840897414 1107 90-91 0.9857262234024162 1107 92-93 1.2689957722111629 1107 94-95 1.337716720394262 1107 96-97 1.79404426119671 1107 98-99 2.12217194906426 1107 100-101 2.283879782905082 1108 1 0.05451611468129869 1108 2 0.11745337423221613 1108 3 0.08169904228876135 1108 4 0.053190293799239896 1108 5 -0.10740423403530741 1108 6 -0.03204256685023665 1108 7 -0.08428922817950024 1108 8 0.006379565594663461 1108 9 0.11668408369475003 1108 10-11 0.1301920334211104 1108 12-13 -0.00917021409622265 1108 14-15 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2316 100-101 -1.217374834697317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 12.0 4 24.0 5 61.0 6 136.0 7 307.0 8 792.0 9 1370.0 10 2384.0 11 3796.0 12 5653.0 13 8146.0 14 10419.0 15 14241.0 16 18610.0 17 24142.0 18 21886.0 19 16424.0 20 15532.0 21 16495.0 22 17850.0 23 19936.0 24 23488.0 25 27588.0 26 33901.0 27 41089.0 28 50770.0 29 61025.0 30 75167.0 31 91819.0 32 113323.0 33 142561.0 34 183243.0 35 240765.0 36 306071.0 37 309709.0 38 122493.0 39 3872.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38021314453895 20.983582910894043 15.857453785256926 21.778750159310082 2 16.453698495931327 21.6253758443381 38.84342901387433 23.077496645856243 3 20.237301041377073 24.853797848706265 30.262252809357726 24.646648300558933 4 13.836855947293621 17.84178210918342 36.488361596874434 31.83300034664852 5 13.492575791623349 37.85331354532187 34.20612950940713 14.447981153647655 6 31.863600293712874 37.07093007925014 16.71353661806512 14.351933008971871 7 28.221817415079798 33.19607309638454 20.30110013940004 18.281009349135626 8 26.350755787225072 36.01982512528968 19.018402484411933 18.61101660307332 9 25.959220920178538 17.06848082595142 20.367862043832748 36.60443621003729 10-11 25.025819629733054 27.425273683137156 26.656425229981735 20.892481457148058 12-13 25.79312650444596 24.428807438077133 27.884814098687947 21.89325195878895 14-15 22.563844774621785 25.876064820278632 26.317794565916763 25.24229583918282 16-17 21.620857564563256 29.348251722007774 27.090296957148436 21.94059375628053 18-19 21.5487132284862 27.433661529983354 29.924937299609354 21.092687941921092 20-21 22.1148340739947 26.419846481996363 30.485760117564304 20.979559326444637 22-23 21.94363241321416 26.286454989877043 30.355809589649894 21.414103007258902 24-25 21.795608702007215 26.573159988464745 30.385201491682366 21.24602981784567 26-27 21.53612011083112 26.83990572145163 30.274391463376695 21.349582704340555 28-29 21.486240212893563 27.01289524033857 30.337381876344256 21.163482670423612 30-31 21.643377664532203 26.673851505915145 30.354538485184047 21.328232344368605 32-33 21.441385566293533 26.730488808739487 30.28716507325292 21.540960551714058 34-35 21.967209633861323 26.72036588683956 30.13095110529962 21.181473373999495 36-37 21.942988691461736 26.5819781374515 30.05068374164921 21.424349429437555 38-39 21.676262044425307 26.833890752439256 30.132012777596444 21.357834425538996 40-41 21.885164586365065 26.74283383544672 30.00668606646464 21.36531551172358 42-43 21.728052110958018 26.772152836422357 29.832214765100673 21.667580287518952 44-45 21.899311986971554 26.970723028462203 29.658013132145612 21.471951852420638 46-47 22.088264965752426 27.287175443075352 29.078858549202586 21.545701041969632 48-49 22.11293032911347 27.33208665023626 28.9387178060221 21.616265214628168 50-51 22.234405391912627 27.520669278215916 28.689521096909942 21.555404232961518 52-53 22.371533948576545 27.628293221049553 28.45234844381062 21.547824386563278 54-55 22.108782400139845 27.60197362410974 28.575922161150004 21.713321814600413 56-57 22.240898875960628 27.810826785942666 28.398129086512462 21.550145251584237 58-59 22.32037615790178 27.435612044203094 28.517431424611146 21.72658037328398 60-61 22.150409830196626 27.58962859740251 28.74662919045759 21.513332381943275 62-63 22.00155349816084 27.608047377249694 28.851364397041735 21.53903472754773 64-65 21.99459090309796 27.52980459797927 28.723716820888974 21.751887678033803 66-67 21.947852631984386 27.637527301026566 28.573058114953927 21.84156195203513 68-69 21.995603195287952 27.56214856795221 28.658288179340648 21.78396005741919 70-71 22.076512500327144 27.58313511335451 28.505777719399518 21.834574666918837 72-73 22.134062184429943 27.57469365785972 28.36996106374731 21.92128309396303 74-75 22.05518573961419 27.949690547139127 28.060326703805455 21.934797009441233 76-77 22.090758661147287 27.685969185825737 28.31734323174035 21.905928921286627 78-79 22.09996805107088 27.722806745520113 28.250976368162274 21.92624883524673 80-81 22.032909865997205 27.931585837192802 28.155796212249523 21.87970808456047 82-83 22.22638936074394 27.873383292907427 28.092137490222726 21.808089856125907 84-85 22.11196977927016 27.935632808256383 27.998049479038073 21.954347933435386 86-87 22.151669022920764 27.875070304927096 28.022074882956804 21.95118578919534 88-89 22.09125246221558 27.89904434679254 27.992422128806034 22.01728106218585 90-91 22.148215273388118 27.86988384955752 28.00512108881163 21.97677978824273 92-93 22.20665608092243 27.714370579894183 28.084583569644312 21.99438976953908 94-95 22.070142466546212 27.714066466611392 28.043925580228603 22.171865486613793 96-97 22.103202448068178 27.6673035054444 27.99911313328135 22.23038091320607 98-99 22.02480271354367 27.747529629386385 27.793082912901628 22.434584744168315 100-101 22.126405276788415 27.51836169073737 28.169046081028494 22.186186951445723 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 632.0 1 455.5 2 403.0 3 574.5 4 488.0 5 401.5 6 754.5 7 1702.0 8 2736.0 9 3214.5 10 2994.0 11 2623.5 12 2680.5 13 2945.0 14 3200.5 15 3424.5 16 3617.5 17 3862.5 18 4085.5 19 4235.5 20 4587.0 21 5248.0 22 6143.5 23 7340.5 24 8968.5 25 11116.5 26 13688.0 27 16672.0 28 19402.5 29 22322.5 30 26187.0 31 29989.0 32 34307.0 33 38191.5 34 41776.0 35 45693.0 36 50219.5 37 54889.5 38 58028.5 39 60192.0 40 61906.5 41 65426.5 42 68301.5 43 70245.5 44 73089.5 45 75450.0 46 76419.0 47 77551.0 48 78929.5 49 76968.5 50 73443.5 51 70009.5 52 66934.5 53 64771.0 54 62731.5 55 57797.0 56 50926.5 57 45190.5 58 40923.5 59 36320.0 60 29931.0 61 24127.0 62 19517.0 63 14809.0 64 10935.0 65 8400.5 66 6211.5 67 4761.5 68 3560.0 69 2425.0 70 1822.5 71 1382.0 72 1012.0 73 854.0 74 708.5 75 553.0 76 401.5 77 249.5 78 160.0 79 108.5 80 69.0 81 39.0 82 24.0 83 13.0 84 6.5 85 5.0 86 4.0 87 1.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03718322044217911 2 0.0 3 0.0 4 4.938010682892311E-5 5 0.015406593330624012 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4690053414461557E-5 12-13 0.00333315721095231 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.2345026707230778E-4 22-23 3.456607478024618E-4 24-25 5.431811751181542E-4 26-27 0.002172724700472617 28-29 0.0028393561426630787 30-31 4.6911101487476963E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009727881045297853 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.876021365784623E-5 74-75 2.4690053414461557E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4814032048676934E-4 84-85 3.456607478024618E-4 86-87 0.0 88-89 0.0 90-91 0.007258875703851697 92-93 0.002789976035834156 94-95 0.0 96-97 0.0 98-99 2.962806409735387E-4 100-101 0.011801845532112625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2025107.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.564871619155966 #Duplication Level Percentage of deduplicated Percentage of total 1 78.85579426272241 46.18179467422914 2 12.36849046425282 14.487181123234425 3 3.6738771157222843 6.454804248804917 4 1.6339314292435398 3.827639375526077 5 0.8992496404796694 2.6332219874131995 6 0.551015550897639 1.9362092999087235 7 0.3468111691703059 1.4217666118983845 8 0.2601463776312735 1.21883513665312 9 0.1931381392848475 1.0179999298781781 >10 1.1021185474341486 11.889744439017855 >50 0.07791226978644467 3.09681982117615 >100 0.03540373519957372 3.6885437957545006 >500 0.0016045866130386195 0.6735348766768696 >1k 2.5335578100609744E-4 0.2577811556204906 >5k 2.5335578100609744E-4 1.2141235242079438 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9903 0.48901119792682557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8665 0.42787862567261875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5980 0.29529303883696023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.11401866666798346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4690053414461557E-5 0.0 18-19 0.0 0.0 0.0 4.938010682892311E-5 0.0 20-21 0.0 0.0 0.0 4.938010682892311E-5 0.0 22-23 0.0 0.0 0.0 9.876021365784623E-5 0.0 24-25 0.0 0.0 0.0 1.4814032048676934E-4 0.0 26-27 0.0 0.0 0.0 1.728303739012309E-4 2.4690053414461557E-5 28-29 0.0 0.0 0.0 2.962806409735387E-4 4.938010682892311E-5 30-31 0.0 0.0 0.0 0.0017283037390123089 4.938010682892311E-5 32-33 0.0 0.0 0.0 0.003925718492899388 4.938010682892311E-5 34-35 0.0 0.0 0.0 0.007604536451654159 4.938010682892311E-5 36-37 0.0 0.0 0.0 0.012789447668691086 4.938010682892311E-5 38-39 0.0 0.0 0.0 0.022072907752528632 9.876021365784623E-5 40-41 0.0 0.0 0.0 0.03209706943880002 9.876021365784623E-5 42-43 0.0 0.0 0.0 0.04276317251384741 9.876021365784623E-5 44-45 0.0 0.0 0.0 0.05505881911424927 9.876021365784623E-5 46-47 0.0 4.938010682892311E-5 0.0 0.07044072239145882 9.876021365784623E-5 48-49 0.0 4.938010682892311E-5 0.0 0.08964958394790991 9.876021365784623E-5 50-51 0.0 9.876021365784623E-5 0.0 0.11105586025824808 9.876021365784623E-5 52-53 0.0 9.876021365784623E-5 0.0 0.1346101712156444 9.876021365784623E-5 54-55 0.0 2.22210480730154E-4 0.0 0.16097914826228935 9.876021365784623E-5 56-57 0.0 2.4690053414461557E-4 0.0 0.19250834647255677 9.876021365784623E-5 58-59 0.0 2.4690053414461557E-4 0.0 0.23161739108106388 9.876021365784623E-5 60-61 0.0 2.715905875590771E-4 0.0 0.2777631009126925 9.876021365784623E-5 62-63 0.0 2.962806409735387E-4 0.0 0.32524207362870206 1.4814032048676934E-4 64-65 0.0 3.2097069438800024E-4 0.0 0.3748937710451843 1.4814032048676934E-4 66-67 0.0 3.456607478024618E-4 0.0 0.4353103317503717 1.728303739012309E-4 68-69 0.0 3.456607478024618E-4 0.0 0.5070102468659681 1.9752042731569245E-4 70-71 0.0 3.456607478024618E-4 0.0 0.5841172836793316 2.22210480730154E-4 72-73 0.0 3.456607478024618E-4 0.0 0.6665573720302187 2.4690053414461557E-4 74-75 0.0 3.456607478024618E-4 0.0 0.7599598440971267 2.4690053414461557E-4 76-77 0.0 3.456607478024618E-4 0.0 0.866373974313456 2.962806409735387E-4 78-79 0.0 3.456607478024618E-4 0.0 0.9918488257657496 2.962806409735387E-4 80-81 0.0 3.456607478024618E-4 0.0 1.1256936053255457 2.962806409735387E-4 82-83 0.0 3.456607478024618E-4 0.0 1.27087111940258 2.962806409735387E-4 84-85 0.0 3.456607478024618E-4 0.0 1.4221223866195714 2.962806409735387E-4 86-87 0.0 3.456607478024618E-4 0.0 1.5847557684606295 3.456607478024618E-4 88-89 0.0 3.456607478024618E-4 0.0 1.7705978005112817 3.950408546313849E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6185 0.0 39.87224 1 TAGATCG 65 1.3279777E-4 36.5411 5 GTATCAA 9755 0.0 35.314552 1 ATCAACG 12230 0.0 27.966043 3 TATCAAC 12365 0.0 27.814383 2 TCAACGC 12330 0.0 27.777758 4 CAACGCA 12605 0.0 27.171736 5 AACGCAG 12970 0.0 26.403814 6 ACGCAGA 15010 0.0 22.817541 7 CGCAGAG 14985 0.0 22.76051 8 GTACATG 8160 0.0 21.894783 1 TACATGG 7985 0.0 21.833138 2 GAGTACT 9125 0.0 21.811996 12-13 GCAGAGT 15800 0.0 21.105433 9 AGTACTT 9455 0.0 20.699026 12-13 CCCGTAG 115 0.0037168302 20.659279 1 GTACTTT 9630 0.0 20.543076 14-15 ATACGTC 305 2.0918378E-10 20.247366 5 ACATGGG 8170 0.0 20.05959 3 AGAGTAC 13620 0.0 19.897238 10-11 >>END_MODULE