FastQCFastQC Report
Thu 26 May 2016
SRR937523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049999
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112700.3695083178715796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80900.2652459886052422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57320.18793448784737307No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53290.17472136876110453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58250.052.6382261
GTATCAA110300.043.5294651
ATCAACG142350.033.7088663
TATCAAC143050.033.6119962
TCAACGC144500.033.044014
CAACGCA147200.032.4066935
AACGCAG149250.031.9860446
ACGCAGA169650.027.7780867
CGCAGAG171500.027.3122398
GTACATG114800.025.2595391
GCAGAGT185350.024.7331399
TACATGG117500.023.8974342
ACATGGG117500.023.047153
GAGTACT112150.021.91967612-13
AGAGTAC173850.021.39524510-11
TGGTATC29900.021.2929232
AGTACTT118500.020.86534112-13
CAGAGTA181050.020.5312810-11
TAGCGCG1404.846664E-420.3618744
GTGGTAT34450.020.1465681
Produced by FastQC (version 0.11.5)