FastQCFastQC Report
Thu 26 May 2016
SRR937523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049999
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131370.4307214526955583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101740.33357387986028847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70490.23111482987371473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.051.9235731
GTATCAA118850.042.7793351
ATCAACG152800.033.0131383
TATCAAC156250.032.4362072
TCAACGC156050.032.3864674
CAACGCA159550.031.7355525
AACGCAG163200.031.1125876
ACGCAGA186900.027.0683387
CGCAGAG188750.026.6016948
GCAGAGT204100.024.0657189
GTACATG114550.023.2296371
TACATGG116200.022.4006232
GAGTACT127950.022.33125112-13
TGGTATC31050.021.5696542
AGTACTT133150.021.54832312-13
GTACTTT133650.021.2166514-15
AGAGTAC191550.021.16618710-11
ACATGGG117200.021.07473
TATACCG2056.8380723E-720.8532755
GTGGTAT34650.020.7072851
Produced by FastQC (version 0.11.5)