FastQCFastQC Report
Thu 26 May 2016
SRR937522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972259
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112580.3787691449500195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83130.2796862588354514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.19288359459925936No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44520.14978506247268492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.050.234551
GTATCAA104650.043.7691351
ATCAACG135000.033.629153
TATCAAC137150.033.2406162
TCAACGC138900.032.5507624
CAACGCA140100.032.2714165
AACGCAG144100.031.3837036
ACGCAGA163450.027.4939697
CGCAGAG164600.027.244168
GTACATG112200.024.7995551
TACATGG111900.024.512752
GCAGAGT178950.024.3957989
ACATGGG114250.023.2595833
GAGTACT107450.021.55131512-13
AGAGTAC166050.021.54188310-11
AGTACTT113700.021.035112-13
CAGAGTA172750.020.54140310-11
CATGGGG59400.020.4097024
GTACTTT115850.019.80350514-15
TGGTATC28950.019.7051142
Produced by FastQC (version 0.11.5)