##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937522_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2972259 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.512531714093555 31.0 31.0 34.0 30.0 34.0 2 31.62855895128924 33.0 31.0 34.0 30.0 34.0 3 31.66130407881682 33.0 31.0 34.0 30.0 34.0 4 35.293468368671775 37.0 35.0 37.0 33.0 37.0 5 35.29222251492888 37.0 35.0 37.0 33.0 37.0 6 35.319660231493955 37.0 35.0 37.0 33.0 37.0 7 35.25112851874618 37.0 35.0 37.0 33.0 37.0 8 35.24291994742046 37.0 35.0 37.0 32.0 37.0 9 36.83502615350816 39.0 37.0 39.0 33.0 39.0 10-11 36.70229646878014 39.0 37.0 39.0 32.0 39.0 12-13 36.558988466348325 39.0 37.0 39.0 32.0 39.0 14-15 37.694522751886694 40.0 37.0 41.0 32.0 41.0 16-17 37.65080835822181 40.0 37.0 41.0 32.0 41.0 18-19 37.616906871171054 40.0 37.0 41.0 32.0 41.0 20-21 37.54787065999295 40.0 37.0 41.0 32.0 41.0 22-23 37.39418755229608 40.0 37.0 41.0 32.0 41.0 24-25 37.22350020640866 40.0 37.0 41.0 31.0 41.0 26-27 37.054945581794854 39.0 36.5 41.0 31.0 41.0 28-29 37.10438777374381 39.0 37.0 41.0 31.0 41.0 30-31 36.93753909063779 39.0 36.0 41.0 30.5 41.0 32-33 36.72557404990615 39.0 36.0 41.0 30.0 41.0 34-35 36.58944392127334 39.0 36.0 41.0 30.0 41.0 36-37 36.5756808205476 39.0 36.0 41.0 30.0 41.0 38-39 36.47860263859913 39.0 35.5 41.0 30.0 41.0 40-41 36.28579608977549 39.0 35.0 41.0 30.0 41.0 42-43 36.036716685860824 39.0 35.0 41.0 29.0 41.0 44-45 35.80918957600936 39.0 35.0 41.0 27.5 41.0 46-47 35.468931711536584 39.0 35.0 40.5 26.5 41.0 48-49 35.147686826753656 39.0 35.0 40.0 25.5 41.0 50-51 34.26475821925344 38.0 34.0 39.5 23.5 40.5 52-53 34.345660489210395 38.0 34.0 39.5 24.0 40.5 54-55 34.6564182327314 38.0 34.0 40.0 24.0 41.0 56-57 34.44078359254695 38.0 34.0 40.0 23.0 41.0 58-59 34.18671774566079 38.0 34.0 40.0 23.0 41.0 60-61 33.89995673324566 38.0 33.5 40.0 22.0 41.0 62-63 33.566312357032146 37.0 33.0 40.0 21.0 41.0 64-65 33.19618663784011 37.0 33.0 40.0 19.5 41.0 66-67 32.819351375502606 36.0 32.0 39.0 18.5 41.0 68-69 32.34142986866219 36.0 31.5 39.0 15.5 40.5 70-71 31.900880946108668 35.0 31.0 38.5 12.5 40.0 72-73 31.512462742984376 35.0 31.0 38.0 9.0 40.0 74-75 31.053494833391035 35.0 31.0 37.0 7.0 39.0 76-77 30.61538227321374 35.0 30.0 37.0 4.5 39.0 78-79 30.128420504404225 34.0 30.0 36.0 2.0 39.0 80-81 29.641287990043935 34.0 29.0 36.0 2.0 37.5 82-83 29.1540330099093 34.0 29.0 35.0 2.0 37.0 84-85 28.704571506049774 34.0 29.0 35.0 2.0 36.5 86-87 28.258171478326755 34.0 27.5 35.0 2.0 36.0 88-89 27.83950675227159 34.0 27.0 35.0 2.0 36.0 90-91 27.40707606571298 33.0 26.0 35.0 2.0 35.0 92-93 26.93365551252431 33.0 25.5 35.0 2.0 35.0 94-95 26.385125253216493 33.0 25.0 35.0 2.0 35.0 96-97 25.734632648096955 32.0 23.5 35.0 2.0 35.0 98-99 24.946270328393318 32.0 19.0 34.0 2.0 35.0 100-101 23.025189594850247 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05601544851841922 1101 2 0.03670814704927494 1101 3 0.006105668346240378 1101 4 0.009268170772820383 1101 5 0.0347635057908775 1101 6 -0.0018190998970979422 1101 7 -0.07367359111393057 1101 8 0.0646144905469086 1101 9 0.20920598980752914 1101 10-11 0.17425545965237887 1101 12-13 0.09299479753665452 1101 14-15 0.07449109269746401 1101 16-17 0.24676102911674036 1101 18-19 0.2722830850644655 1101 20-21 0.08019329648055873 1101 22-23 0.25504302704735693 1101 24-25 0.2608578063463085 1101 26-27 0.2310370299874407 1101 28-29 0.1187274346006646 1101 30-31 0.09045339489014026 1101 32-33 0.09854094520505896 1101 34-35 0.10560138819801779 1101 36-37 0.10803083178388562 1101 38-39 0.1566329491613203 1101 40-41 0.11363511951783778 1101 42-43 0.3886802452140543 1101 44-45 0.2811356786539392 1101 46-47 0.4354416316282226 1101 48-49 0.5390757874281746 1101 50-51 0.38998627704963695 1101 52-53 0.4459686094887587 1101 54-55 0.6092747611206235 1101 56-57 0.5474355787071374 1101 58-59 0.5787628392346633 1101 60-61 0.4350315158195528 1101 62-63 0.5208364750608467 1101 64-65 0.5292528954635642 1101 66-67 0.5937714605168196 1101 68-69 0.7415370790056954 1101 70-71 0.5896214794660892 1101 72-73 0.47620449129178155 1101 74-75 0.37318297584183213 1101 76-77 0.513404631311797 1101 78-79 0.4569047572710687 1101 80-81 0.3636098704041686 1101 82-83 0.37841626286463637 1101 84-85 0.39755456649435317 1101 86-87 0.45457827279571816 1101 88-89 0.6638135977621538 1101 90-91 0.6609941474176644 1101 92-93 0.7714516642975333 1101 94-95 0.8766136145201671 1101 96-97 0.9318953307901552 1101 98-99 1.0629877404477774 1101 100-101 1.3264751295656865 1102 1 0.11133271226945851 1102 2 -0.037601518188868255 1102 3 0.12474761748060814 1102 4 -0.057925624598787806 1102 5 -1.8818292867450737E-4 1102 6 -0.0810873452537777 1102 7 0.045876454045775006 1102 8 0.03746413509043123 1102 9 0.010091128119583459 1102 10-11 0.06947617028755815 1102 12-13 -0.029955195089904407 1102 14-15 0.04721451066795623 1102 16-17 0.1609776134910348 1102 18-19 0.14354331646351426 1102 20-21 0.025911887203761808 1102 22-23 0.08520067954356136 1102 24-25 0.07724685625237271 1102 26-27 0.04588174475010476 1102 28-29 0.08112631547341209 1102 30-31 0.08614290039652417 1102 32-33 0.06659735010069312 1102 34-35 0.07331638902115145 1102 36-37 0.113233797168796 1102 38-39 0.20739298427599806 1102 40-41 0.09775875783336119 1102 42-43 0.11711071786108818 1102 44-45 -0.006451551465275429 1102 46-47 0.07896686203257275 1102 48-49 0.005931916504444246 1102 50-51 -0.03789739760606636 1102 52-53 0.01418937445446744 1102 54-55 -0.022828189925931497 1102 56-57 0.06936938614585131 1102 58-59 0.05505624289539668 1102 60-61 0.08694203057478944 1102 62-63 0.0018114956798953585 1102 64-65 0.12889927723399808 1102 66-67 0.22430393240485813 1102 68-69 0.1409905669645397 1102 70-71 0.13705652690725856 1102 72-73 0.15735014245954204 1102 74-75 0.2847861799542848 1102 76-77 0.29766013350360154 1102 78-79 0.23162085981933345 1102 80-81 0.23381709031904663 1102 82-83 0.2220931285567005 1102 84-85 0.3644049765673145 1102 86-87 0.32660913989057505 1102 88-89 0.33180584769566934 1102 90-91 0.3396631637776899 1102 92-93 0.2892855591144432 1102 94-95 0.2542705511197063 1102 96-97 0.41147940810454386 1102 98-99 0.5365888211760037 1102 100-101 1.0535519345546192 1103 1 -0.15489102184054104 1103 2 -0.18151840184024692 1103 3 -0.14395397199041682 1103 4 -0.16695537079628053 1103 5 -0.2199411239897131 1103 6 -0.13614365255451588 1103 7 -0.2442185153827836 1103 8 -0.28064857921926745 1103 9 -0.2061051994887464 1103 10-11 -0.10620951598741613 1103 12-13 -0.10439281818381829 1103 14-15 -0.08888257013521184 1103 16-17 -0.17449580034607948 1103 18-19 -0.19178205983558172 1103 20-21 -0.3310663490903991 1103 22-23 -0.43939204817370836 1103 24-25 -0.2951677904609866 1103 26-27 -0.15894771308909839 1103 28-29 -0.17671642556921796 1103 30-31 -0.1980761787185088 1103 32-33 -0.2760843581875321 1103 34-35 -0.13348973126037578 1103 36-37 -0.06053780948332843 1103 38-39 -0.2351316570462103 1103 40-41 -0.046328902664392047 1103 42-43 -0.14469283944183786 1103 44-45 -0.16048736103635974 1103 46-47 -0.2404248223548464 1103 48-49 -0.15782438387505948 1103 50-51 -0.1473858413967548 1103 52-53 -0.1926526779119655 1103 54-55 -0.30812848006094384 1103 56-57 -0.2759508774551094 1103 58-59 -0.24315420668526144 1103 60-61 -0.17335019141496133 1103 62-63 -0.26230059863559774 1103 64-65 -0.13152374550026735 1103 66-67 -0.25988528728019844 1103 68-69 -0.15330865528481752 1103 70-71 -0.1044185527996504 1103 72-73 -0.06575562011874325 1103 74-75 -0.11036028373190376 1103 76-77 -0.09602644006846717 1103 78-79 -0.08177082934134816 1103 80-81 -0.17006391958733502 1103 82-83 -0.22791208225856607 1103 84-85 -0.19367168102219168 1103 86-87 -0.14116309471120303 1103 88-89 -0.12012020486912789 1103 90-91 0.009163890772605043 1103 92-93 0.03593485904870519 1103 94-95 0.10800787246365573 1103 96-97 0.2040823972827539 1103 98-99 0.15264706880932621 1103 100-101 0.26380634167123773 1104 1 0.03596689733081604 1104 2 -0.03322948408791504 1104 3 0.06173478327175275 1104 4 0.03965924283569677 1104 5 -0.036290438036473915 1104 6 0.008766838048977377 1104 7 0.016435000947709 1104 8 0.08098534036098215 1104 9 0.15850872511420988 1104 10-11 0.1737210439845711 1104 12-13 0.10409080944346272 1104 14-15 0.19704217171625515 1104 16-17 0.2565124371793175 1104 18-19 0.24189041025544356 1104 20-21 0.20201442480183118 1104 22-23 0.17600239883497437 1104 24-25 0.09247099237925482 1104 26-27 0.21439882208201766 1104 28-29 0.1821077303994798 1104 30-31 0.1869662600332802 1104 32-33 0.058002186095208685 1104 34-35 0.12332034786351187 1104 36-37 0.15879340838262834 1104 38-39 0.18956347231970483 1104 40-41 0.1983057942519224 1104 42-43 0.27633595450635795 1104 44-45 0.2785837061041718 1104 46-47 0.20797509045658558 1104 48-49 0.24549917325745696 1104 50-51 0.15535846575776446 1104 52-53 0.14428310153943613 1104 54-55 0.2776470502007342 1104 56-57 0.2527730038513809 1104 58-59 0.36827318626325933 1104 60-61 0.31955435725607373 1104 62-63 0.09022686856106787 1104 64-65 0.1425221710403406 1104 66-67 0.25465581977798024 1104 68-69 0.4186499441888998 1104 70-71 0.33609478683092675 1104 72-73 0.39736490754899734 1104 74-75 0.32228556822361654 1104 76-77 0.30067223562502576 1104 78-79 0.4520158806698973 1104 80-81 0.4270794191289582 1104 82-83 0.4170419441068738 1104 84-85 0.5222032395307288 1104 86-87 0.5962865758020897 1104 88-89 0.45489123017753386 1104 90-91 0.5342400669762348 1104 92-93 0.7255175608046116 1104 94-95 0.9566082773755049 1104 96-97 0.9157098986707624 1104 98-99 1.021543429765856 1104 100-101 1.2263439648280254 1105 1 -0.0035877261322383447 1105 2 -0.05013845530991645 1105 3 -0.05042651819768906 1105 4 -0.01648832354239005 1105 5 -0.025374932972930253 1105 6 -0.07859061172106863 1105 7 -0.04680329274805928 1105 8 -0.03907258974490446 1105 9 0.059172695342667225 1105 10-11 0.019626651554339958 1105 12-13 -0.20197930377702988 1105 14-15 -0.11411372463966529 1105 16-17 -0.1392625149259814 1105 18-19 -0.05680004380555914 1105 20-21 -0.10221769342500409 1105 22-23 -0.13001089921662157 1105 24-25 -0.003174479412933806 1105 26-27 -0.017581882903911605 1105 28-29 0.0022270208877728237 1105 30-31 -0.17732823250820218 1105 32-33 -0.17839631103188935 1105 34-35 -0.005332697536374553 1105 36-37 -0.10673252623661256 1105 38-39 0.0945451040241565 1105 40-41 -0.027952409227182784 1105 42-43 0.14001995106210074 1105 44-45 0.08115769547886487 1105 46-47 0.07412379702284255 1105 48-49 0.1322648906154953 1105 50-51 0.14265546807290974 1105 52-53 0.08746200833012807 1105 54-55 0.1363905550614959 1105 56-57 0.03246553375412731 1105 58-59 0.13056609497264304 1105 60-61 0.26888425875896615 1105 62-63 0.22020171643723785 1105 64-65 0.18630345458921482 1105 66-67 0.19396935268503057 1105 68-69 0.14117106923413303 1105 70-71 0.204467540179472 1105 72-73 0.1883126680892815 1105 74-75 0.12399499583166929 1105 76-77 0.13633110608508048 1105 78-79 0.09552779233425923 1105 80-81 0.19848557804865408 1105 82-83 0.219358662742426 1105 84-85 0.32420732905745453 1105 86-87 0.21052954621177733 1105 88-89 0.17664617596226506 1105 90-91 0.34541606716115325 1105 92-93 0.5603029344629213 1105 94-95 0.48763285590892735 1105 96-97 0.6449419149216453 1105 98-99 0.7505082690430278 1105 100-101 1.0439865024323822 1106 1 -0.07335037813832201 1106 2 -0.15947938859903132 1106 3 -0.15662033352598215 1106 4 -0.17896624249114268 1106 5 -0.15406119618688763 1106 6 -0.158718866614997 1106 7 -0.07089023439489495 1106 8 -0.2922028205370921 1106 9 -0.1406281886823635 1106 10-11 -0.5561986266990928 1106 12-13 -0.4872801216468261 1106 14-15 -0.5207572097690729 1106 16-17 -0.5974187652287242 1106 18-19 -0.34762197050343246 1106 20-21 -0.3792543821757093 1106 22-23 -0.41643957425268496 1106 24-25 -0.29439934258042655 1106 26-27 -0.44472675570072795 1106 28-29 -0.2548258105363459 1106 30-31 -0.2250927429654439 1106 32-33 -0.1444793783840268 1106 34-35 -0.22681886259905326 1106 36-37 -0.18775168710158852 1106 38-39 -0.3224181663508858 1106 40-41 -0.17847368195037916 1106 42-43 -0.34164147958697555 1106 44-45 -0.2719942066267862 1106 46-47 -0.055531570701390365 1106 48-49 -0.1149754341649043 1106 50-51 -0.14600028941646315 1106 52-53 -0.3499923647962859 1106 54-55 -0.365294711378219 1106 56-57 -0.3651750003342613 1106 58-59 -0.25986786478507184 1106 60-61 -0.4244849032406677 1106 62-63 -0.3401941970935596 1106 64-65 -0.16464770771910509 1106 66-67 0.0047528084884902455 1106 68-69 -0.27357311438041165 1106 70-71 -0.08330792802640019 1106 72-73 -0.029823434394664616 1106 74-75 -0.26487648611325554 1106 76-77 -0.007231691024578879 1106 78-79 0.06482045021800431 1106 80-81 -0.12685770259007612 1106 82-83 -0.13512201734134877 1106 84-85 -0.1494285825251822 1106 86-87 -0.09023387988353804 1106 88-89 -0.037918340121816385 1106 90-91 0.08615655723010462 1106 92-93 0.11773367351575814 1106 94-95 0.1919780803304434 1106 96-97 0.21662227247045251 1106 98-99 0.484453813663837 1106 100-101 0.6273876166802843 1107 1 -0.0485836004776381 1107 2 -0.03146737503519148 1107 3 -0.018747184096351788 1107 4 -0.0445284449282255 1107 5 -0.0806168768117459 1107 6 -0.016167647016573028 1107 7 0.004638130218388881 1107 8 0.07896683257437331 1107 9 0.01302442622954203 1107 10-11 0.011846679753247713 1107 12-13 0.0035127645285513154 1107 14-15 -0.0031261604722345737 1107 16-17 0.04942581350904618 1107 18-19 -0.06127794274841136 1107 20-21 -0.1775720339989988 1107 22-23 0.03592503956296156 1107 24-25 -0.06716075834359003 1107 26-27 -0.014098846693229916 1107 28-29 0.04361292325199173 1107 30-31 0.01238265021534346 1107 32-33 0.025978509481319634 1107 34-35 0.08276363189941804 1107 36-37 0.03646835431614903 1107 38-39 0.16900624667071895 1107 40-41 0.020454948671073225 1107 42-43 0.045774256469755414 1107 44-45 0.0020922990606777603 1107 46-47 0.06343059603578638 1107 48-49 0.01844097521919963 1107 50-51 0.01962033407192365 1107 52-53 0.047526039728865044 1107 54-55 0.05734713760585919 1107 56-57 0.028170728283441804 1107 58-59 0.12082370196903724 1107 60-61 0.13609143362184994 1107 62-63 0.07691572791936352 1107 64-65 0.08322976929426318 1107 66-67 0.15540266376829237 1107 68-69 0.13689059365972867 1107 70-71 0.3244446936772505 1107 72-73 0.08365920855216302 1107 74-75 0.10335742776224421 1107 76-77 0.04139777011814516 1107 78-79 0.00245124299652133 1107 80-81 0.1596107963617186 1107 82-83 0.22746241274073142 1107 84-85 0.3158881464967038 1107 86-87 0.320204375690853 1107 88-89 0.1836381971147425 1107 90-91 0.2885789541240271 1107 92-93 0.3920519428437821 1107 94-95 0.4939768365488497 1107 96-97 0.6131325466938549 1107 98-99 0.8701988246150414 1107 100-101 1.0166133021656911 1108 1 -0.1037190719694756 1108 2 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sequence quality scores pass #Quality Count 2 12818.0 3 3141.0 4 2738.0 5 3063.0 6 3490.0 7 4072.0 8 5063.0 9 6136.0 10 7887.0 11 10069.0 12 12809.0 13 15987.0 14 17648.0 15 21096.0 16 25327.0 17 31078.0 18 27233.0 19 20527.0 20 20269.0 21 21132.0 22 22789.0 23 25771.0 24 28790.0 25 32769.0 26 37492.0 27 43860.0 28 52232.0 29 61993.0 30 75066.0 31 92728.0 32 115383.0 33 150180.0 34 199867.0 35 277757.0 36 403166.0 37 558995.0 38 459618.0 39 62213.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75755628659883 19.238180582393802 15.187058125593204 22.81720500541417 2 14.913114350222234 20.28662646089049 40.81263140842836 23.98762778045891 3 18.353240287977606 24.979262885863868 30.321609186558433 26.34588763960009 4 12.37447369735455 16.990412821063174 38.08038408112519 32.554729400457084 5 13.399732508878405 37.33748800660392 34.67577247932816 14.587007005189518 6 31.042658503340796 36.72245568812642 18.048577993685967 14.186307814846813 7 27.666899650658877 33.032173706930415 21.481562402115532 17.819364240295172 8 25.99937283146363 34.64387954317853 20.440434162477114 18.916313462880726 9 26.11663166866944 16.8717994365255 20.83564293141606 36.17592596338899 10-11 24.212133020797378 26.931927652231863 27.982141541286094 20.87379778568467 12-13 25.34750462979823 24.35275555609508 28.366163424796298 21.933576389310396 14-15 22.416327796772475 25.634209337298618 26.744707897523046 25.20475496840586 16-17 21.636169936917096 28.874055218994638 27.323123154209267 22.166651689879004 18-19 21.630191178111808 27.292470041704416 29.912154026824478 21.1651847533593 20-21 22.257764567772785 26.433092995453293 30.060345193793065 21.248797242980856 22-23 22.015432734564993 26.293840965356758 29.940310846170625 21.750415453907625 24-25 21.732293578043507 26.565675736994322 30.014152124891996 21.687878560070175 26-27 21.60923155549802 26.831875627042944 29.744051857125903 21.81484096033313 28-29 21.59427629635724 26.96689091406892 29.822015987742823 21.616816801831025 30-31 21.72487238536265 26.706353635782076 29.764130853015793 21.804643125839483 32-33 21.46434975253597 26.77361024351877 29.661997263606725 22.100042740338534 34-35 22.00598416195176 26.77055063901036 29.52485012195112 21.698615077086764 36-37 21.989984370385454 26.603013184705905 29.456968663903954 21.95003378100469 38-39 21.69000171936579 26.85855013636931 29.455449883858524 21.995998260406378 40-41 22.03314920852177 26.767336921435398 29.418009395590783 21.781504474452053 42-43 21.760579596589825 26.761602838510175 29.31304898109042 22.164768583809582 44-45 21.86110039677509 26.97096205147017 29.217201993855518 21.950735557899222 46-47 22.075355338695417 27.245426301908243 28.5852674624599 22.093950896936438 48-49 22.063473356172803 27.33234968161146 28.394319234269116 22.209857727946616 50-51 22.181981339726313 27.429226425192144 28.3286182986959 22.060173936385645 52-53 22.322059504579364 27.35325476698756 28.222478870636046 22.10220685779703 54-55 22.1246119375782 27.415867190214783 28.275442420244985 22.184078451962034 56-57 22.09100355731525 27.70353852156933 28.14545129697472 22.060006624140694 58-59 22.207544759145584 27.244716366126088 28.179240197722677 22.368498677005654 60-61 22.01117913533705 27.32178249278571 28.505227610168653 22.16181076170859 62-63 21.94982247539241 27.369704456042115 28.58885341337266 22.091619655192815 64-65 21.91668885914909 27.330868001361885 28.429581286849352 22.32286185263967 66-67 21.870628902122633 27.495685680571984 28.29874995554637 22.33493546175901 68-69 22.0314684701812 27.329545224717965 28.37076947346706 22.268216831633776 70-71 21.934230766767207 27.275831832872548 28.348409859923656 22.44152754043659 72-73 22.01993399609182 27.290284878045824 28.148733481113673 22.541047644748687 74-75 21.972419303453403 27.651848068491557 28.057450513366405 22.318282114688635 76-77 22.120787227113322 27.368366755917922 28.100340575249195 22.410505441719557 78-79 22.107547479271144 27.499957437347355 28.04256804672486 22.34992703665664 80-81 22.056889215835177 27.611748961665526 27.969146054918998 22.3622157675803 82-83 22.092472709761445 27.603918487715497 27.937133889080012 22.36647491344305 84-85 22.10632427182077 27.513888399644777 27.936460476819462 22.443326851714986 86-87 22.133131934938117 27.43273339668326 27.940487920309877 22.493646748068745 88-89 22.129290229848248 27.394586872498373 27.829433088628402 22.64668980902498 90-91 22.07281242715226 27.304786598999353 27.961530831143932 22.660870142704457 92-93 22.114766178465388 27.145313591340205 28.091535042891774 22.64838518730263 94-95 21.933554540663323 27.139904506794565 28.097550106628855 22.828990845913257 96-97 21.929801645735836 27.091067611417845 28.06838653808783 22.910744204758487 98-99 21.814946391271576 27.21248096960936 27.948817182841427 23.023755456277637 100-101 22.00331277064996 26.887660738308313 28.316250946758707 22.792775544283028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4203.0 1 2357.0 2 524.5 3 556.5 4 508.0 5 484.0 6 704.0 7 1365.0 8 2328.5 9 2931.0 10 2923.0 11 2793.5 12 2995.5 13 3312.5 14 3551.5 15 3697.5 16 3833.5 17 4180.5 18 4617.0 19 5000.0 20 5633.0 21 6710.0 22 7902.0 23 9417.5 24 11658.5 25 14849.5 26 18960.0 27 24065.0 28 28497.0 29 32945.5 30 38909.5 31 45342.0 32 51906.0 33 57404.0 34 62432.0 35 67867.0 36 74616.0 37 81173.5 38 85476.0 39 88366.0 40 91291.0 41 96159.0 42 100134.0 43 103537.5 44 108525.5 45 112297.0 46 112930.5 47 112578.0 48 112480.5 49 110281.0 50 106452.0 51 101526.5 52 96613.5 53 94920.0 54 93552.0 55 86500.0 56 76837.5 57 68372.5 58 62607.0 59 56173.0 60 46082.0 61 37341.5 62 30357.5 63 23118.0 64 17015.5 65 12847.5 66 9395.5 67 7001.0 68 5325.0 69 3745.5 70 2700.0 71 2047.0 72 1598.5 73 1323.5 74 1043.0 75 777.0 76 584.5 77 387.5 78 240.5 79 157.5 80 116.0 81 76.5 82 49.0 83 35.5 84 23.5 85 19.5 86 18.5 87 17.5 88 17.5 89 16.5 90 12.0 91 10.5 92 11.5 93 10.5 94 8.5 95 10.0 96 11.5 97 12.5 98 12.5 99 10.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35616007891640666 2 0.3084859024735058 3 0.30189159154703543 4 0.30734199139442425 5 0.3085195469170082 6 0.2288158602598226 7 0.2149879939803362 8 0.22033746049721775 9 0.21448332732780018 10-11 0.2373110822441786 12-13 0.22498039370054895 14-15 0.21932812719214576 16-17 0.22065708271049056 18-19 0.21485341620632656 20-21 0.20714883864427694 22-23 0.20947030524594257 24-25 0.19917510553420814 26-27 0.20035266105679217 28-29 0.21825150500006896 30-31 0.23167563795752658 32-33 0.2246271270437738 34-35 0.23320646013688578 36-37 0.20506288314712817 38-39 0.2035152387460178 40-41 0.20099190548333776 42-43 0.20846097194087057 44-45 0.21354128290973295 46-47 0.218587949435093 48-49 0.2280588602810186 50-51 0.23288683792361298 52-53 0.25388097066911064 54-55 0.22624206033188898 56-57 0.19636579450175773 58-59 0.19789661668111694 60-61 0.19347237236055137 62-63 0.20356570541127136 64-65 0.19476768343539375 66-67 0.19125183908939294 68-69 0.18864439471795694 70-71 0.20011714995227534 72-73 0.19915828331245697 74-75 0.20368346096352974 76-77 0.20526474980814258 78-79 0.20333019430675453 80-81 0.20491148315136737 82-83 0.19713961670231295 84-85 0.19057895021934496 86-87 0.19069670577160333 88-89 0.19153781685916335 90-91 0.19794708334637057 92-93 0.19254715016423535 94-95 0.19044437244533535 96-97 0.18916588359224415 98-99 0.20021808328278254 100-101 0.18699581698633935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2972259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.95143587142624 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50113987464528 39.44942330420436 2 13.746073273448792 14.557486348458998 3 4.916400119448537 7.809913369297547 4 2.238757450391934 4.7418168626442485 5 1.2410343549328466 3.285727552973172 6 0.7596600079570426 2.4135052917254693 7 0.4910439981279235 1.820103934384364 8 0.35210073845816053 1.4915391738199288 9 0.24688309494701333 1.176553293284341 >10 1.3580948521357108 13.178198595274068 >50 0.10028091250170353 3.63284363366235 >100 0.04503147400079098 4.088355400473534 >500 0.002545344730587355 0.8837165243344073 >1k 7.636034189952518E-4 0.6182005974342907 >5k 1.2726723649920864E-4 0.47327876991126877 >10k+ 6.363361824960432E-5 0.37933734811768927 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11258 0.3787691449500195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8313 0.2796862588354514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5733 0.19288359459925936 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4452 0.14978506247268492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.3644443502400025E-5 0.0 9 0.0 0.0 0.0 3.3644443502400025E-5 0.0 10-11 0.0 0.0 0.0 5.046666525360004E-5 0.0 12-13 0.0 0.0 0.0 6.728888700480005E-5 2.1868888276560018E-4 14-15 0.0 0.0 0.0 6.728888700480005E-5 2.691555480192002E-4 16-17 0.0 0.0 0.0 8.411110875600006E-5 2.691555480192002E-4 18-19 3.3644443502400025E-5 0.0 0.0 1.0093333050720008E-4 2.691555480192002E-4 20-21 3.3644443502400025E-5 0.0 0.0 1.6822221751200013E-4 3.0279999152160026E-4 22-23 3.3644443502400025E-5 0.0 0.0 2.0186666101440015E-4 3.0279999152160026E-4 24-25 3.3644443502400025E-5 0.0 0.0 3.532666567752003E-4 3.0279999152160026E-4 26-27 3.3644443502400025E-5 0.0 0.0 4.5419998728240033E-4 3.3644443502400025E-4 28-29 5.046666525360004E-5 0.0 0.0 8.747555310624007E-4 3.3644443502400025E-4 30-31 6.728888700480005E-5 0.0 0.0 0.0017999777273784013 3.3644443502400025E-4 32-33 6.728888700480005E-5 0.0 0.0 0.0031962221327280025 3.532666567752003E-4 34-35 1.0093333050720008E-4 0.0 0.0 0.004912088751350404 4.3737776553120036E-4 36-37 2.0186666101440015E-4 0.0 0.0 0.007469066457532807 4.3737776553120036E-4 38-39 2.0186666101440015E-4 0.0 0.0 0.012246577434873611 4.3737776553120036E-4 40-41 2.0186666101440015E-4 0.0 0.0 0.017225955073228813 4.3737776553120036E-4 42-43 2.3551110451680018E-4 0.0 0.0 0.02181842161130642 4.7102220903360036E-4 44-45 3.0279999152160026E-4 0.0 0.0 0.02588939927509682 4.878444307848004E-4 46-47 3.3644443502400025E-4 0.0 0.0 0.031171576904973623 5.046666525360004E-4 48-49 3.3644443502400025E-4 0.0 0.0 0.03660515453061122 5.383110960384004E-4 50-51 3.3644443502400025E-4 0.0 0.0 0.04247610992178004 5.383110960384004E-4 52-53 3.3644443502400025E-4 0.0 0.0 0.05813759837214724 5.383110960384004E-4 54-55 3.3644443502400025E-4 0.0 1.6822221751200013E-5 0.08019153108797046 5.383110960384004E-4 56-57 4.037333220288003E-4 0.0 3.3644443502400025E-5 0.1132976634943321 5.383110960384004E-4 58-59 4.037333220288003E-4 0.0 3.3644443502400025E-5 0.16137557325926172 5.383110960384004E-4 60-61 4.037333220288003E-4 0.0 3.3644443502400025E-5 0.22143090491104578 5.383110960384004E-4 62-63 4.3737776553120036E-4 0.0 3.3644443502400025E-5 0.2594491260687578 5.383110960384004E-4 64-65 4.3737776553120036E-4 0.0 3.3644443502400025E-5 0.3104541024183962 5.719555395408005E-4 66-67 5.383110960384004E-4 0.0 3.3644443502400025E-5 0.3997800999172683 5.719555395408005E-4 68-69 5.383110960384004E-4 0.0 3.3644443502400025E-5 0.5162403411008261 5.719555395408005E-4 70-71 5.719555395408005E-4 0.0 3.3644443502400025E-5 0.6425079375653333 6.392444265456005E-4 72-73 6.055999830432005E-4 0.0 3.3644443502400025E-5 0.7850089780197487 7.233555353016005E-4 74-75 6.392444265456005E-4 0.0 3.3644443502400025E-5 0.9308239961591503 7.738222005552006E-4 76-77 6.392444265456005E-4 0.0 3.3644443502400025E-5 1.0750072587886856 8.074666440576006E-4 78-79 6.392444265456005E-4 0.0 3.3644443502400025E-5 1.2199643436187761 8.074666440576006E-4 80-81 7.401777570528005E-4 0.0 3.3644443502400025E-5 1.3656447839841683 8.074666440576006E-4 82-83 8.074666440576006E-4 0.0 3.3644443502400025E-5 1.5704721560267796 8.074666440576006E-4 84-85 8.411110875600007E-4 0.0 3.3644443502400025E-5 1.849216370444164 8.242888658088006E-4 86-87 8.747555310624007E-4 0.0 3.3644443502400025E-5 2.1702852947875675 8.915777528136007E-4 88-89 8.747555310624007E-4 0.0 3.3644443502400025E-5 2.5571122839564118 9.083999745648007E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5700 0.0 50.23455 1 GTATCAA 10465 0.0 43.769135 1 ATCAACG 13500 0.0 33.62915 3 TATCAAC 13715 0.0 33.240616 2 TCAACGC 13890 0.0 32.550762 4 CAACGCA 14010 0.0 32.271416 5 AACGCAG 14410 0.0 31.383703 6 ACGCAGA 16345 0.0 27.493969 7 CGCAGAG 16460 0.0 27.24416 8 GTACATG 11220 0.0 24.799555 1 TACATGG 11190 0.0 24.51275 2 GCAGAGT 17895 0.0 24.395798 9 ACATGGG 11425 0.0 23.259583 3 GAGTACT 10745 0.0 21.551315 12-13 AGAGTAC 16605 0.0 21.541883 10-11 AGTACTT 11370 0.0 21.0351 12-13 CAGAGTA 17275 0.0 20.541403 10-11 CATGGGG 5940 0.0 20.409702 4 GTACTTT 11585 0.0 19.803505 14-15 TGGTATC 2895 0.0 19.705114 2 >>END_MODULE