FastQCFastQC Report
Thu 26 May 2016
SRR937522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972259
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131080.4410113654294595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103770.34912839022440506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72930.24536892646300337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.051.1522831
GTATCAA115200.042.1722371
ATCAACG146700.032.7378043
TCAACGC150550.031.932164
TATCAAC151350.031.9203072
CAACGCA153600.031.2362385
AACGCAG158150.030.3635166
ACGCAGA182400.026.1474957
CGCAGAG184250.025.8849548
GCAGAGT199200.023.6322759
GTACATG110250.022.7658331
GAGTACT124150.021.59923712-13
TACATGG113150.021.5792872
AGTACTT128100.021.52654512-13
AGAGTAC185100.021.09534510-11
ACATGGG112400.020.7934863
GTACTTT131350.020.15973714-15
TAAGGTG13850.019.8932975
CAGAGTA195200.019.73614110-11
TGGTATC31450.019.3338282
Produced by FastQC (version 0.11.5)