##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937522_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2972259 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09699222039533 31.0 31.0 34.0 31.0 34.0 2 32.326739695295736 34.0 31.0 34.0 31.0 34.0 3 32.40483652333124 34.0 31.0 34.0 31.0 34.0 4 36.04411560365365 37.0 35.0 37.0 35.0 37.0 5 35.88312593216136 37.0 35.0 37.0 35.0 37.0 6 35.96645278893932 37.0 35.0 37.0 35.0 37.0 7 35.92478279988386 37.0 35.0 37.0 35.0 37.0 8 35.898873550387094 37.0 35.0 37.0 35.0 37.0 9 37.586623507574544 39.0 37.0 39.0 35.0 39.0 10-11 37.5061421296058 39.0 37.0 39.0 35.0 39.0 12-13 37.41737648031346 39.0 37.0 39.0 34.0 39.0 14-15 38.619181235551814 40.0 38.0 41.0 34.0 41.0 16-17 38.593799867373605 40.0 38.0 41.0 34.0 41.0 18-19 38.600470719409046 40.0 38.0 41.0 34.0 41.0 20-21 38.50632313671184 40.0 38.0 41.0 34.0 41.0 22-23 38.37577058392287 40.0 38.0 41.0 34.0 41.0 24-25 38.22981291334302 40.0 38.0 41.0 33.5 41.0 26-27 38.00080477508858 40.0 37.0 41.0 33.0 41.0 28-29 37.972881905648194 40.0 37.0 41.0 33.0 41.0 30-31 38.09164241743402 40.0 37.0 41.0 33.5 41.0 32-33 38.04761849488891 40.0 37.0 41.0 33.0 41.0 34-35 37.93124337414741 40.0 37.0 41.0 33.0 41.0 36-37 37.76218879310316 40.0 37.0 41.0 33.0 41.0 38-39 37.61418570858058 40.0 37.0 41.0 33.0 41.0 40-41 37.44177156163039 40.0 37.0 41.0 32.5 41.0 42-43 37.23194630750551 40.0 36.5 41.0 32.0 41.0 44-45 36.95162972002103 40.0 36.0 41.0 31.0 41.0 46-47 36.62561893159378 39.5 36.0 41.0 30.5 41.0 48-49 36.309310696005966 39.0 36.0 41.0 30.0 41.0 50-51 36.0954703139935 39.0 35.0 41.0 29.5 41.0 52-53 35.85281683056557 39.0 35.0 40.0 28.5 41.0 54-55 35.64293118466459 39.0 35.0 40.0 28.0 41.0 56-57 35.3939658354134 38.5 35.0 40.0 28.0 41.0 58-59 35.13695273527644 38.0 34.5 40.0 27.0 41.0 60-61 34.8059334667672 38.0 34.0 40.0 26.5 41.0 62-63 34.43460596805326 37.5 34.0 40.0 26.0 41.0 64-65 34.089623077935 37.0 33.5 40.0 26.0 41.0 66-67 33.70450758160712 36.0 33.0 39.0 25.5 41.0 68-69 33.26716985296369 36.0 33.0 39.0 24.0 40.0 70-71 32.88140686932061 35.0 32.0 38.5 23.5 40.0 72-73 32.45431858394575 35.0 32.0 38.0 22.0 40.0 74-75 32.0147616678089 35.0 31.5 37.0 21.5 39.0 76-77 30.702700706768823 34.0 29.5 36.0 20.0 38.5 78-79 31.33474421307161 34.5 31.0 36.0 20.5 39.0 80-81 31.1699570932412 34.5 31.0 36.0 20.0 37.5 82-83 30.83335385644387 34.0 31.0 35.5 18.5 37.0 84-85 30.491623206456772 34.0 31.0 35.0 18.0 37.0 86-87 30.152919042384934 34.0 30.5 35.0 15.5 36.0 88-89 29.782347702538708 34.0 30.0 35.0 8.5 36.0 90-91 29.336681964795126 34.0 29.5 35.0 4.5 35.5 92-93 28.911299452705837 34.0 29.0 35.0 2.0 35.0 94-95 28.498336282268806 34.0 29.0 35.0 2.0 35.0 96-97 28.06781878698996 33.5 29.0 35.0 2.0 35.0 98-99 27.404816841331794 33.0 27.0 35.0 2.0 35.0 100-101 25.797489216114748 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 3.6101978801639234E-4 1101 2 0.07795201631262927 1101 3 0.1235275746039477 1101 4 0.05668810890995246 1101 5 -0.08384035037321524 1101 6 -0.05394472883532586 1101 7 0.031392993042246076 1101 8 -0.023378567506171066 1101 9 0.07873266706380377 1101 10-11 0.09845456922001716 1101 12-13 0.13301464844424515 1101 14-15 0.11139122636862453 1101 16-17 0.09933310669865136 1101 18-19 0.09066267562832309 1101 20-21 0.07023090177013103 1101 22-23 0.007889437668708865 1101 24-25 0.057182886376558884 1101 26-27 0.03521364125102622 1101 28-29 -0.03642100005870219 1101 30-31 0.04017467358827531 1101 32-33 0.06604921979264589 1101 34-35 0.124072068313545 1101 36-37 0.14442796218334308 1101 38-39 0.1790544458155452 1101 40-41 0.1990039211251684 1101 42-43 0.07938637703460927 1101 44-45 0.13807260167977375 1101 46-47 0.1364746397949972 1101 48-49 0.09775905174655009 1101 50-51 0.1061049725142098 1101 52-53 0.21966732427544855 1101 54-55 0.13389329077919854 1101 56-57 0.14874298462513735 1101 58-59 0.1125417802309272 1101 60-61 0.1471731095493496 1101 62-63 0.01615523493041593 1101 64-65 0.20813038023825925 1101 66-67 0.2031045596212948 1101 68-69 0.037944755197202085 1101 70-71 -0.041917022958521954 1101 72-73 -0.07173790286178416 1101 74-75 0.03817430754485329 1101 76-77 0.01600212994367567 1101 78-79 0.10514460512110801 1101 80-81 -0.01561000420765879 1101 82-83 -0.061628327653838966 1101 84-85 -0.07390845041992833 1101 86-87 -0.23297952310386805 1101 88-89 -0.03751929879971172 1101 90-91 0.03979097252047836 1101 92-93 0.16571899950099933 1101 94-95 -0.0910821784579845 1101 96-97 -0.09849802883209335 1101 98-99 -0.28799236500796965 1101 100-101 -0.04634958676298595 1102 1 0.06529356210149473 1102 2 0.04098907667956553 1102 3 0.011789687963585038 1102 4 0.0027003091780102295 1102 5 -0.10631276537350942 1102 6 -0.05623498541024219 1102 7 -0.007839982404028945 1102 8 -0.02011934837071294 1102 9 0.026507341601458734 1102 10-11 0.046595055719286904 1102 12-13 -0.009865355836836898 1102 14-15 0.01679135439260193 1102 16-17 -0.06625501721907767 1102 18-19 -0.06584898153867158 1102 20-21 0.014629321854620514 1102 22-23 -0.01698444809582611 1102 24-25 0.06483215542666443 1102 26-27 0.06014512554889251 1102 28-29 -0.003830442705073267 1102 30-31 -0.01335570800404895 1102 32-33 0.05210618760159491 1102 34-35 0.0909559754058833 1102 36-37 -0.008939775039891629 1102 38-39 -0.07418271430715606 1102 40-41 -0.05716136869144606 1102 42-43 0.04594756894024954 1102 44-45 0.06741942205690776 1102 46-47 0.03441718750476497 1102 48-49 0.062049799338751654 1102 50-51 -0.020151625275232732 1102 52-53 6.709603360519623E-4 1102 54-55 0.009442774659575548 1102 56-57 0.010735366912726363 1102 58-59 -0.05408914266506315 1102 60-61 -0.10674011846445097 1102 62-63 -0.08879960741095516 1102 64-65 -0.03632647580502635 1102 66-67 -0.040608417491654336 1102 68-69 -0.06472932819976052 1102 70-71 -0.13042064165787082 1102 72-73 -0.0792447074544711 1102 74-75 -0.21707236736535052 1102 76-77 -0.20042538025963808 1102 78-79 -0.04608933111772373 1102 80-81 0.0255029914512086 1102 82-83 -0.04878834822590861 1102 84-85 -0.028562064687044142 1102 86-87 0.061780664789900186 1102 88-89 0.05703890615221141 1102 90-91 0.02836930591304565 1102 92-93 -0.09905676042177447 1102 94-95 -0.16548436899965324 1102 96-97 -0.0667876873626625 1102 98-99 -0.04663183863090481 1102 100-101 0.08624448369268833 1103 1 0.016766552178673066 1103 2 -0.049471869652215616 1103 3 -0.05710232829840578 1103 4 -0.002735645129106956 1103 5 -0.11182283789267444 1103 6 -0.11402610888588072 1103 7 -0.06084710013902139 1103 8 -0.07501416017542795 1103 9 -0.13624113672115357 1103 10-11 -0.062371577295856184 1103 12-13 -0.012902014784074822 1103 14-15 -0.07605191568701031 1103 16-17 -0.21387867331269206 1103 18-19 -0.11682514093180885 1103 20-21 -0.1506871735181008 1103 22-23 -0.1670684575755388 1103 24-25 -0.1945578530140608 1103 26-27 -0.20159100937330265 1103 28-29 -0.22965802756777265 1103 30-31 -0.13124125463448166 1103 32-33 -0.01934622174413647 1103 34-35 -0.07281862249286775 1103 36-37 0.011868150596868077 1103 38-39 0.04154087435082232 1103 40-41 0.05260936074048317 1103 42-43 0.024332431004808086 1103 44-45 0.030191992113195454 1103 46-47 -0.059710555167413304 1103 48-49 -0.22504291843421242 1103 50-51 -0.18980920709100246 1103 52-53 -0.08882306817572783 1103 54-55 -0.1730700740440767 1103 56-57 -0.0863795120730515 1103 58-59 -0.20769204353785398 1103 60-61 -0.20307032557380467 1103 62-63 -0.08686855617604294 1103 64-65 -0.21715953268671484 1103 66-67 -0.20509582309701102 1103 68-69 -0.17436289253264192 1103 70-71 -0.18189328699917695 1103 72-73 -0.25334149284551444 1103 74-75 -0.45840630425163553 1103 76-77 -0.6274258361065321 1103 78-79 -0.35444714732736315 1103 80-81 -0.37050338934456306 1103 82-83 -0.31436782403115515 1103 84-85 -0.32465517307226577 1103 86-87 -0.21778308787749623 1103 88-89 -0.23390815753349514 1103 90-91 -0.31894565246805584 1103 92-93 -0.22629320123076013 1103 94-95 -0.34748985931615195 1103 96-97 -0.2944045839814109 1103 98-99 -0.40051454569849554 1103 100-101 -0.20646205883724633 1104 1 -0.012282042835479956 1104 2 0.07275431056359594 1104 3 1.0971043548124726E-4 1104 4 0.019717363234306617 1104 5 -0.04812335286969471 1104 6 0.017281910889174412 1104 7 0.020985761291363758 1104 8 -0.0010999954007289148 1104 9 -0.05145499647890972 1104 10-11 0.04371067102484716 1104 12-13 0.011303608748825411 1104 14-15 0.06129749577061716 1104 16-17 -0.006180380109640282 1104 18-19 0.004188410622660399 1104 20-21 -0.016954481665848675 1104 22-23 -0.10401820488603164 1104 24-25 -0.15093440178497275 1104 26-27 -0.11059268330546956 1104 28-29 -0.17884012098049595 1104 30-31 -0.20852454765740447 1104 32-33 -0.15514567554102143 1104 34-35 -0.009606777020046309 1104 36-37 -0.009231217455052843 1104 38-39 -0.11896488049816156 1104 40-41 -0.01013201238330197 1104 42-43 0.011770624203705893 1104 44-45 0.015133658093830604 1104 46-47 0.12940612860793266 1104 48-49 -0.029929029011015018 1104 50-51 0.07296949819250642 1104 52-53 0.13375271818937762 1104 54-55 0.20117637361332186 1104 56-57 0.1966494675930548 1104 58-59 0.1536643396241928 1104 60-61 -0.04850286165972051 1104 62-63 0.1143072085219714 1104 64-65 0.09406774531959172 1104 66-67 -0.05693068476644214 1104 68-69 -0.11543374352250169 1104 70-71 0.03646187143913693 1104 72-73 -0.01483971350413782 1104 74-75 -0.031084560599826006 1104 76-77 0.052516994512625814 1104 78-79 0.027968872220359486 1104 80-81 0.14576038782204748 1104 82-83 0.10682379821863108 1104 84-85 0.05717134169588434 1104 86-87 0.09423352373057625 1104 88-89 0.1651021728297799 1104 90-91 0.187427833518111 1104 92-93 0.03516450743429189 1104 94-95 -0.07150463429381304 1104 96-97 -0.09362197420008656 1104 98-99 0.09553416935601078 1104 100-101 0.32199553576996465 1105 1 -0.12761217392992563 1105 2 -0.033536478600069586 1105 3 -0.06105837447376672 1105 4 -0.018386870942862288 1105 5 -0.21083694634074845 1105 6 -0.08142866642481295 1105 7 -0.04478542061760038 1105 8 -0.0787959528606379 1105 9 -0.10795724490279923 1105 10-11 -0.07484287110471399 1105 12-13 -0.1108898918677852 1105 14-15 -0.03830114633713322 1105 16-17 -0.11970378883330568 1105 18-19 -0.13266680276636578 1105 20-21 -0.0776280318058653 1105 22-23 -0.20724126628934414 1105 24-25 -0.1971748595073919 1105 26-27 -0.16913998644965034 1105 28-29 -0.25285406701637925 1105 30-31 -0.29207586671139296 1105 32-33 -0.21863939469693605 1105 34-35 -0.19569549065275993 1105 36-37 -0.23021852718008518 1105 38-39 -0.15817171743163527 1105 40-41 -0.09002793087869065 1105 42-43 -0.22551972539508824 1105 44-45 -0.05948981272832299 1105 46-47 0.021667766636838337 1105 48-49 -0.08940101116891697 1105 50-51 -0.0032238194501275075 1105 52-53 -0.08101250502236468 1105 54-55 0.025801349794662087 1105 56-57 0.05353856418913949 1105 58-59 -0.048370498077701995 1105 60-61 0.03010981097826715 1105 62-63 0.07820558764833407 1105 64-65 0.18396342489990758 1105 66-67 -0.038617941833031466 1105 68-69 -0.04121749663738683 1105 70-71 -0.1514032631409421 1105 72-73 -0.035726135028404826 1105 74-75 -0.04129167277122647 1105 76-77 0.02389448362402291 1105 78-79 -3.482935554295352E-4 1105 80-81 -0.07210140777426588 1105 82-83 -0.1106029000159019 1105 84-85 -0.03458408774618604 1105 86-87 0.07414601088889228 1105 88-89 0.13132880844137063 1105 90-91 0.0305497891719817 1105 92-93 0.08660690374854951 1105 94-95 0.1430287636048604 1105 96-97 0.005781651054441284 1105 98-99 -0.2731012934487005 1105 100-101 -0.1932348142911131 1106 1 -0.26273055729506467 1106 2 -0.08896746502172448 1106 3 -0.19914969576963415 1106 4 -0.11599450657071486 1106 5 -0.23427308504086852 1106 6 -0.20303824384481572 1106 7 -0.20768495477062032 1106 8 -0.1525169704648306 1106 9 -0.23899603336887765 1106 10-11 -0.3305222349315713 1106 12-13 -0.45595376915904495 1106 14-15 -0.3067063685795759 1106 16-17 -0.16422871661660565 1106 18-19 -0.22857709547243132 1106 20-21 -0.5063785127086362 1106 22-23 -0.3831887094393096 1106 24-25 -0.3264018107119071 1106 26-27 -0.23564069438364754 1106 28-29 -0.24451084357504982 1106 30-31 -0.22577027358824608 1106 32-33 -0.2725054481968172 1106 34-35 -0.27944433459501283 1106 36-37 -0.24483329337341786 1106 38-39 -0.28423910924460216 1106 40-41 -0.3003327996783298 1106 42-43 -0.471476525828038 1106 44-45 -0.18052360073457407 1106 46-47 -0.13941634712256956 1106 48-49 -0.21236915964647096 1106 50-51 -0.2095140873847967 1106 52-53 -0.07416358035347059 1106 54-55 -0.05261768475828177 1106 56-57 -0.10934438138959024 1106 58-59 -0.1391284459622284 1106 60-61 -0.04424479063252562 1106 62-63 -0.21356049434407964 1106 64-65 -0.26200347465707097 1106 66-67 0.043003682873170135 1106 68-69 0.008170968888968844 1106 70-71 -0.03673507510294627 1106 72-73 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0.15046879188990658 1107 28-29 -0.0516328887464681 1107 30-31 -0.043062838318242314 1107 32-33 -0.02553329478929811 1107 34-35 0.0051396518299853255 1107 36-37 -0.002996623507577567 1107 38-39 0.012879219733896718 1107 40-41 -0.09750552250336852 1107 42-43 -0.02021288068139171 1107 44-45 0.028153091678291275 1107 46-47 0.06327655698251533 1107 48-49 -0.028235688510967805 1107 50-51 0.020104767589174344 1107 52-53 -0.026508357195154986 1107 54-55 -0.05919352533094724 1107 56-57 -0.10527677166587068 1107 58-59 -0.02110728944737872 1107 60-61 -0.15225651577938493 1107 62-63 -0.034087449435695305 1107 64-65 0.02501088997030365 1107 66-67 0.05958493425461597 1107 68-69 -0.19474761817620845 1107 70-71 -0.3131865335292403 1107 72-73 -0.09184142795181316 1107 74-75 -0.01442311723058154 1107 76-77 -0.04634631091837704 1107 78-79 -0.004201873773507003 1107 80-81 -0.011737300601357248 1107 82-83 0.05280332795067366 1107 84-85 -0.0379934411793279 1107 86-87 -0.13707840322652132 1107 88-89 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-0.2791403334782103 2316 38-39 -1.0122329651025481 2316 40-41 -0.29911553937075297 2316 42-43 0.01669769678230182 2316 44-45 -0.026329901959321944 2316 46-47 -0.39945559169925104 2316 48-49 -0.5003803850566442 2316 50-51 -0.4103372493391291 2316 52-53 -0.3625579448600931 2316 54-55 -0.7398139676567084 2316 56-57 -0.3846061206236442 2316 58-59 -0.44131168647530217 2316 60-61 -0.6027785290524577 2316 62-63 -0.337141770714787 2316 64-65 -0.29347632918685207 2316 66-67 -0.3698378262686788 2316 68-69 -0.2725777918887218 2316 70-71 -0.33208682810565193 2316 72-73 -0.0781360940583582 2316 74-75 -0.30435085018519104 2316 76-77 -0.22610995173567616 2316 78-79 -0.2843652847848226 2316 80-81 -0.41656400903247004 2316 82-83 -0.5746055206798637 2316 84-85 -0.5633413759926071 2316 86-87 -0.5808406640233237 2316 88-89 -0.32412898615416097 2316 90-91 -0.19998320589125385 2316 92-93 -0.13740980868680452 2316 94-95 -0.03422605710311544 2316 96-97 0.08756482617215511 2316 98-99 -0.07058783846823857 2316 100-101 -0.14725956528790718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 24.0 5 53.0 6 126.0 7 343.0 8 806.0 9 1609.0 10 2800.0 11 4628.0 12 6811.0 13 9525.0 14 12122.0 15 16192.0 16 21020.0 17 28444.0 18 25545.0 19 16191.0 20 13552.0 21 13802.0 22 14785.0 23 16181.0 24 18585.0 25 22416.0 26 27476.0 27 34782.0 28 43425.0 29 54581.0 30 67443.0 31 85460.0 32 108347.0 33 142717.0 34 192892.0 35 274726.0 36 423464.0 37 655365.0 38 559565.0 39 56441.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.95495539768462 20.06707008800635 15.70374449413347 22.274230020175555 2 15.568326986309067 21.16282598521865 39.6635353783099 23.605311650162385 3 18.650090722241906 25.27000507021764 30.646824519666694 25.433079687873768 4 13.026724790807261 17.771028702411197 37.448183351450865 31.754063155330677 5 13.397196000670277 37.754256707329795 34.446135096157406 14.402412195842523 6 31.135476417095546 37.28820402259696 17.5089384875275 14.067381072779996 7 27.646278470348644 33.42696581960051 21.19902740642723 17.727728303623607 8 26.192838511044965 35.11450381679389 19.97352855185231 18.71912912030883 9 26.150749312223464 16.901689926752685 20.641505333148963 36.306055427874895 10-11 24.35213418480691 27.241939548336802 27.637396337264015 20.768529929592273 12-13 25.507597667411886 24.557954431323555 28.213663671721683 21.720784229542875 14-15 22.45251843799615 25.901830896970957 26.65191694263521 24.99373372239768 16-17 21.595543995324768 29.106548251683318 27.330424434748117 21.9674833182438 18-19 21.607655994985635 27.42087752110432 30.016630448423236 20.954836035486814 20-21 22.2247455554849 26.486302169494653 30.295879329493157 20.99307294552729 22-23 22.033924536738517 26.32885345253514 30.18357187881292 21.453650131913427 24-25 21.837023110656983 26.616089832478394 30.23273496939265 21.31415208747197 26-27 21.65458717162842 26.87811712963407 30.007481027008577 21.459814671728935 28-29 21.62133108850809 27.00455458622979 30.037810770518096 21.336303554744028 30-31 21.739565624201983 26.724141121498786 30.05002323180089 21.486270022498342 32-33 21.554800574243362 26.779143405739543 29.8822377188529 21.7838183011642 34-35 22.05484111579778 26.790986249852384 29.715495856855007 21.438676777494827 36-37 22.083000882665985 26.675723791994976 29.641410845773002 21.59986447956604 38-39 21.797900240390682 26.882827626928467 29.654049380182318 21.665222752498533 40-41 22.11370012754617 26.80908817777198 29.58341085579073 21.49380083889112 42-43 21.906162472526216 26.891737473771197 29.41072853814566 21.791371515556925 44-45 22.016843420584046 27.063140705964837 29.328135490234107 21.591880383217006 46-47 22.21334008913759 27.329953412539084 28.685218885702763 21.771487612620568 48-49 22.253199419431287 27.369730353152384 28.477961872758016 21.89910835465831 50-51 22.32090512988692 27.52659299317337 28.38826098066504 21.764240896274668 52-53 22.55988431665355 27.48273488969557 28.183937328318283 21.773443465332594 54-55 22.32841159818562 27.555464565996623 28.210723968107747 21.905399867710003 56-57 22.360601818347593 27.809790465770313 28.074202147255672 21.755405568626422 58-59 22.42984208307553 27.406662743724553 28.101504613157868 22.06199056004204 60-61 22.22671375542979 27.455312609029026 28.43098464837687 21.886988987164308 62-63 22.175708779080157 27.563126228232466 28.491696046676957 21.769468946010427 64-65 22.115973069641644 27.45314254242312 28.377523627651563 22.053360760283677 66-67 22.087593981547368 27.615796604535475 28.25460365331554 22.042005760601615 68-69 22.236975310698025 27.44762485368873 28.325980340205888 21.989419495407365 70-71 22.133199024714873 27.407537499255618 28.236418831602496 22.222844644427017 72-73 22.266817932084653 27.423417676588752 28.07008070292663 22.239683688399968 74-75 22.18803946762378 27.795306532842528 27.88941004131874 22.127243958214947 76-77 22.29255593136399 27.49984103000445 28.063452747556656 22.1441502910749 78-79 22.291058753628132 27.629843159697725 27.94917939520076 22.12991869147339 80-81 22.231827710842158 27.777693666669023 27.886146530299012 22.104332092189814 82-83 22.261077986142467 27.795899128134078 27.800979461323188 22.142043424400267 84-85 22.211029831701996 27.70878700785514 27.814598836069887 22.265584324372973 86-87 22.235158510748896 27.634317870683546 27.847371309162494 22.283152309405068 88-89 22.235023932974883 27.5468086731338 27.710118801894453 22.50804859199686 90-91 22.225334550001143 27.488230353977656 27.814082660734062 22.472352435287135 92-93 22.171004930699365 27.312306224860748 27.95326349717969 22.563425347260193 94-95 21.981815851175824 27.368123033692555 27.96368015035029 22.686380964781332 96-97 22.068298893198744 27.280361502816547 27.89839310773388 22.752946496250832 98-99 21.87763743956757 27.433728508731427 27.734782483624638 22.95385156807637 100-101 21.76854302067608 27.426710415565754 27.990732043713233 22.814014520044935 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 461.0 1 351.0 2 319.5 3 417.0 4 368.0 5 332.5 6 634.0 7 1479.5 8 2474.5 9 3101.0 10 3129.0 11 2994.5 12 3204.0 13 3533.5 14 3849.5 15 4091.5 16 4318.0 17 4572.5 18 4915.0 19 5329.5 20 5892.0 21 6885.5 22 8175.0 23 9752.5 24 11876.0 25 14957.5 26 19147.0 27 24170.5 28 28549.5 29 33324.0 30 39450.5 31 45605.5 32 52256.5 33 57568.5 34 62018.5 35 67546.0 36 74385.5 37 81042.0 38 85771.5 39 88823.5 40 91528.5 41 96486.0 42 100642.5 43 103940.0 44 109014.0 45 113105.0 46 113208.5 47 112693.5 48 113072.0 49 110810.5 50 107395.5 51 102439.0 52 97145.0 53 95228.5 54 94033.0 55 87094.0 56 76596.0 57 67613.5 58 62173.0 59 55605.0 60 44888.0 61 36018.0 62 29875.5 63 22788.0 64 16021.5 65 11879.0 66 8696.5 67 6712.5 68 5046.5 69 3381.0 70 2455.0 71 1881.5 72 1494.0 73 1314.0 74 1034.5 75 747.5 76 522.5 77 317.0 78 203.0 79 132.5 80 77.0 81 32.5 82 22.5 83 16.5 84 10.5 85 9.5 86 5.0 87 5.0 88 4.0 89 0.5 90 0.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04498262096270884 2 0.0 3 0.0 4 0.0 5 0.01214564410436641 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006577488704719206 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014971777358568012 24-25 5.214888742872004E-4 26-27 0.002472866597426402 28-29 0.0030279999152160023 30-31 0.001345777740096001 32-33 0.0 34-35 0.0 36-37 4.5419998728240033E-4 38-39 4.710222090336004E-4 40-41 6.728888700480005E-5 42-43 0.011270888573304008 44-45 1.6822221751200013E-5 46-47 0.0 48-49 3.3644443502400025E-5 50-51 1.6822221751200013E-5 52-53 2.5233332626800023E-4 54-55 3.3644443502400025E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.373777655312003E-4 84-85 5.046666525360004E-5 86-87 0.0 88-89 0.0 90-91 0.006964399804996805 92-93 0.0021532443841536016 94-95 0.0 96-97 0.0 98-99 0.0011607333008328009 100-101 0.010042866385466408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2972259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.810707399765 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52740343108444 36.85168674201543 2 14.690593880561902 14.928789343880183 3 5.301361353138854 8.080977616042816 4 2.511498773188291 5.10444117197356 5 1.3158063500063715 3.3428525722463265 6 0.8243850479672095 2.5132552474201866 7 0.538123244177058 1.913969589342495 8 0.37222470049560863 1.5130400275078026 9 0.2895078461281959 1.3239088613600356 >10 1.4655457864211525 13.654653481819526 >50 0.11148052580305598 3.8778624889755697 >100 0.04821926808183142 4.264711860554237 >500 0.002787781098975592 0.9965690794787714 >1k 8.628846257713107E-4 0.5952636045637342 >5k 6.637574044394696E-5 0.2459635959254002 >10k+ 1.3275148088789392E-4 0.7920547168940112 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13108 0.4410113654294595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10377 0.34912839022440506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7293 0.24536892646300337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.3644443502400025E-5 5 0.0 0.0 0.0 0.0 3.3644443502400025E-5 6 0.0 0.0 0.0 0.0 3.3644443502400025E-5 7 3.3644443502400025E-5 0.0 0.0 0.0 3.3644443502400025E-5 8 3.3644443502400025E-5 0.0 0.0 0.0 3.3644443502400025E-5 9 3.3644443502400025E-5 0.0 0.0 0.0 3.3644443502400025E-5 10-11 3.3644443502400025E-5 0.0 0.0 0.0 3.3644443502400025E-5 12-13 6.728888700480005E-5 0.0 0.0 0.0 1.1775555225840009E-4 14-15 6.728888700480005E-5 0.0 0.0 0.0 1.5139999576080013E-4 16-17 6.728888700480005E-5 0.0 0.0 1.6822221751200013E-5 1.6822221751200013E-4 18-19 1.0093333050720008E-4 0.0 0.0 3.3644443502400025E-5 1.6822221751200013E-4 20-21 1.345777740096001E-4 0.0 0.0 1.0093333050720008E-4 2.0186666101440015E-4 22-23 1.345777740096001E-4 0.0 0.0 1.345777740096001E-4 2.0186666101440015E-4 24-25 1.6822221751200013E-4 0.0 0.0 2.8597776977040023E-4 2.0186666101440015E-4 26-27 1.8504443926320013E-4 0.0 0.0 3.8691110027760033E-4 2.1868888276560018E-4 28-29 2.691555480192002E-4 0.0 0.0 8.747555310624007E-4 2.3551110451680018E-4 30-31 2.691555480192002E-4 0.0 0.0 0.0018840888361344016 2.3551110451680018E-4 32-33 2.691555480192002E-4 0.0 0.0 0.003280333241484003 2.3551110451680018E-4 34-35 3.0279999152160026E-4 0.0 0.0 0.005013022081857604 2.691555480192002E-4 36-37 3.0279999152160026E-4 0.0 0.0 0.007603644231542406 2.691555480192002E-4 38-39 3.3644443502400025E-4 0.0 0.0 0.01246526631763921 3.0279999152160026E-4 40-41 3.3644443502400025E-4 0.0 0.0 0.017579221730004015 3.0279999152160026E-4 42-43 3.532666567752003E-4 0.0 0.0 0.02208757715932562 3.0279999152160026E-4 44-45 3.700888785264003E-4 0.0 0.0 0.02627631037537442 3.1962221327280023E-4 46-47 4.3737776553120036E-4 0.0 0.0 0.03170988800101202 3.532666567752003E-4 48-49 4.3737776553120036E-4 0.0 0.0 0.037328510065912826 3.700888785264003E-4 50-51 4.3737776553120036E-4 0.0 0.0 0.04318264323533043 3.700888785264003E-4 52-53 4.3737776553120036E-4 0.0 0.0 0.058877776129200046 4.037333220288003E-4 54-55 4.3737776553120036E-4 0.0 0.0 0.08145319771931046 4.037333220288003E-4 56-57 4.3737776553120036E-4 0.0 0.0 0.11486213011719369 4.037333220288003E-4 58-59 4.3737776553120036E-4 0.0 0.0 0.1630577954343817 4.2055554378000033E-4 60-61 4.3737776553120036E-4 0.0 0.0 0.22361779373870178 4.7102220903360036E-4 62-63 4.3737776553120036E-4 0.0 0.0 0.2618547037791794 4.7102220903360036E-4 64-65 4.3737776553120036E-4 0.0 0.0 0.31339799122485623 4.7102220903360036E-4 66-67 4.7102220903360036E-4 0.0 0.0 0.40338005537202515 4.7102220903360036E-4 68-69 5.046666525360004E-4 0.0 0.0 0.5209842076346645 4.7102220903360036E-4 70-71 5.046666525360004E-4 0.0 0.0 0.648446181843507 4.7102220903360036E-4 72-73 5.214888742872004E-4 0.0 0.0 0.7923098222597694 5.046666525360004E-4 74-75 5.383110960384004E-4 0.0 0.0 0.9397229514655352 5.383110960384004E-4 76-77 5.383110960384004E-4 0.0 0.0 1.0854875029396833 5.383110960384004E-4 78-79 5.383110960384004E-4 0.0 0.0 1.2319249432838792 5.887777612920005E-4 80-81 6.055999830432005E-4 0.0 0.0 1.379068916941626 6.055999830432005E-4 82-83 6.055999830432005E-4 0.0 0.0 1.5862009333641516 6.392444265456005E-4 84-85 6.224222047944005E-4 0.0 0.0 1.8695207920978623 6.392444265456005E-4 86-87 6.392444265456005E-4 0.0 0.0 2.1959223607363962 6.392444265456005E-4 88-89 6.392444265456005E-4 0.0 0.0 2.5912445718895967 6.392444265456005E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6245 0.0 51.152283 1 GTATCAA 11520 0.0 42.172237 1 ATCAACG 14670 0.0 32.737804 3 TCAACGC 15055 0.0 31.93216 4 TATCAAC 15135 0.0 31.920307 2 CAACGCA 15360 0.0 31.236238 5 AACGCAG 15815 0.0 30.363516 6 ACGCAGA 18240 0.0 26.147495 7 CGCAGAG 18425 0.0 25.884954 8 GCAGAGT 19920 0.0 23.632275 9 GTACATG 11025 0.0 22.765833 1 GAGTACT 12415 0.0 21.599237 12-13 TACATGG 11315 0.0 21.579287 2 AGTACTT 12810 0.0 21.526545 12-13 AGAGTAC 18510 0.0 21.095345 10-11 ACATGGG 11240 0.0 20.793486 3 GTACTTT 13135 0.0 20.159737 14-15 TAAGGTG 1385 0.0 19.893297 5 CAGAGTA 19520 0.0 19.736141 10-11 TGGTATC 3145 0.0 19.333828 2 >>END_MODULE