FastQCFastQC Report
Thu 26 May 2016
SRR937521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243069
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121740.3753851675681276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87220.26894278228431157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.18679836907571193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49030.15118395569135287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62050.049.6599121
GTATCAA115650.044.1602361
ATCAACG148000.034.2020723
TATCAAC151750.033.5761262
TCAACGC150850.033.5590064
CAACGCA153900.032.8311465
AACGCAG159150.031.7289816
ACGCAGA183200.027.4340347
CGCAGAG184750.027.074898
GCAGAGT200700.024.448699
GTACATG123450.023.8436261
TACATGG121900.023.4333972
ACATGGG125500.022.1173763
AGAGTAC185300.021.36663810-11
GAGTACT118800.021.05092412-13
AGTACTT124050.020.86839112-13
CAGAGTA196650.019.97641610-11
GTACTTT126500.019.5248914-15
TGGTATC32900.019.5030442
GTGGTAT37200.018.9187091
Produced by FastQC (version 0.11.5)