##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937521_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3243069 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.435118401736133 31.0 31.0 34.0 30.0 34.0 2 31.53314591826446 33.0 31.0 34.0 30.0 34.0 3 31.577469982908166 33.0 31.0 34.0 28.0 34.0 4 35.219801983861586 37.0 35.0 37.0 33.0 37.0 5 35.20685807178324 37.0 35.0 37.0 33.0 37.0 6 35.251333227877666 37.0 35.0 37.0 32.0 37.0 7 35.18303835040204 37.0 35.0 37.0 32.0 37.0 8 35.16197959402036 37.0 35.0 37.0 32.0 37.0 9 36.74919435880026 39.0 37.0 39.0 33.0 39.0 10-11 36.63884703039004 39.0 37.0 39.0 32.0 39.0 12-13 36.5145650616746 39.0 37.0 39.0 32.0 39.0 14-15 37.62649715439295 40.0 37.0 41.0 32.0 41.0 16-17 37.561598442709666 40.0 37.0 41.0 32.0 41.0 18-19 37.51277339458396 40.0 37.0 41.0 32.0 41.0 20-21 37.43680168383713 40.0 37.0 41.0 32.0 41.0 22-23 37.29086491838441 40.0 37.0 41.0 31.5 41.0 24-25 37.134140840049966 39.5 36.5 41.0 31.0 41.0 26-27 36.95219296906726 39.0 36.0 41.0 31.0 41.0 28-29 36.75246194268453 39.0 36.0 40.5 30.0 41.0 30-31 36.738702290947245 39.0 36.0 41.0 30.0 41.0 32-33 36.5745230829193 39.0 36.0 41.0 30.0 41.0 34-35 36.35835623602212 39.0 35.0 40.5 30.0 41.0 36-37 36.34389323816422 39.0 35.0 41.0 30.0 41.0 38-39 36.303867416943646 39.0 35.0 41.0 30.0 41.0 40-41 36.12765454574047 39.0 35.0 41.0 29.5 41.0 42-43 35.88658798193933 39.0 35.0 41.0 28.5 41.0 44-45 35.66498708476446 39.0 35.0 41.0 27.0 41.0 46-47 35.345575441040566 39.0 35.0 40.0 26.5 41.0 48-49 35.035578798970974 39.0 34.5 40.0 24.5 41.0 50-51 34.10814308915413 37.5 33.5 39.5 23.5 40.5 52-53 34.23760934472871 38.0 33.5 39.5 24.0 40.5 54-55 34.58210078169783 38.0 34.0 40.0 24.0 41.0 56-57 34.34636188745907 38.0 34.0 40.0 23.0 41.0 58-59 34.09130841804476 38.0 34.0 40.0 22.5 41.0 60-61 33.80551508463125 38.0 33.0 40.0 22.0 41.0 62-63 33.483914927496144 37.0 33.0 40.0 20.5 41.0 64-65 33.121459950435835 37.0 32.5 40.0 19.5 41.0 66-67 32.73051267179329 36.0 32.0 39.0 18.0 41.0 68-69 32.232052725366 35.5 31.5 39.0 14.5 40.5 70-71 31.803334279967522 35.0 31.0 38.5 12.0 40.0 72-73 31.397202310527465 35.0 31.0 38.0 8.0 40.0 74-75 30.93648238751627 35.0 30.5 37.0 7.0 39.0 76-77 30.493596497638503 35.0 30.0 36.5 4.0 39.0 78-79 30.010878430277 34.0 29.5 36.0 2.0 39.0 80-81 29.557905181789224 34.0 29.0 36.0 2.0 37.5 82-83 29.105195880815366 34.0 29.0 35.0 2.0 37.0 84-85 28.635694306843302 34.0 28.5 35.0 2.0 36.5 86-87 28.1937222118925 34.0 27.5 35.0 2.0 36.0 88-89 27.793768957737257 34.0 26.5 35.0 2.0 36.0 90-91 27.344507008639038 33.0 26.0 35.0 2.0 35.0 92-93 26.88436817101332 33.0 25.5 35.0 2.0 35.0 94-95 26.323440389334916 33.0 24.5 35.0 2.0 35.0 96-97 25.671427280763993 32.0 23.0 35.0 2.0 35.0 98-99 24.93232459747233 32.0 18.0 34.0 2.0 35.0 100-101 23.08980721655938 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10544858038915095 1101 2 0.006766998244447819 1101 3 0.10516379340413451 1101 4 0.02409620665790868 1101 5 0.1610022844858392 1101 6 -0.0014547945032745702 1101 7 -0.01887470746639508 1101 8 -0.042510750960339294 1101 9 -0.041698015204154615 1101 10-11 -0.019196413510123023 1101 12-13 0.10095379541271399 1101 14-15 0.11389251787972654 1101 16-17 0.014770915194922907 1101 18-19 0.19242068414430236 1101 20-21 0.12769316432541444 1101 22-23 0.13909560361013007 1101 24-25 0.11490787521256607 1101 26-27 0.2534605824645979 1101 28-29 0.23370763067704559 1101 30-31 0.1229993089424326 1101 32-33 0.05702103580151885 1101 34-35 -0.06795433492249003 1101 36-37 -0.024798647180148237 1101 38-39 0.10281437529358328 1101 40-41 0.0339573663407009 1101 42-43 0.08677456892331747 1101 44-45 -0.027104077762338363 1101 46-47 0.04944709284348647 1101 48-49 0.03756789441163022 1101 50-51 0.04506727595904181 1101 52-53 0.09835499514450419 1101 54-55 0.17345491832467275 1101 56-57 0.16392487238624653 1101 58-59 0.16661713899802777 1101 60-61 0.12276560942181192 1101 62-63 0.24714708008616526 1101 64-65 0.2354723646230994 1101 66-67 0.2771497087235204 1101 68-69 0.09961152129408646 1101 70-71 0.1595933017338922 1101 72-73 0.26702000102881485 1101 74-75 0.26886902440612914 1101 76-77 0.2556861563582835 1101 78-79 0.2743091930315238 1101 80-81 0.41905377984202374 1101 82-83 0.30238864725181 1101 84-85 0.19938813317710924 1101 86-87 0.4762447167390107 1101 88-89 0.4751801673099614 1101 90-91 0.3237474222461998 1101 92-93 0.08351127161124339 1101 94-95 0.215578956210976 1101 96-97 0.15121605861649456 1101 98-99 0.28506171249787116 1101 100-101 0.6058461437587042 1102 1 -0.007169762926793055 1102 2 -0.13601602705156424 1102 3 0.002491952282841936 1102 4 -0.12331597090992119 1102 5 -0.13201438163920187 1102 6 -0.01871202596142041 1102 7 0.07278242727497286 1102 8 0.09052804554699634 1102 9 0.17912001723261284 1102 10-11 0.042231445495758635 1102 12-13 0.1757024113611152 1102 14-15 0.23647765759815798 1102 16-17 0.2505429369513834 1102 18-19 0.21791104503081726 1102 20-21 0.21070135569625137 1102 22-23 0.2581375150610512 1102 24-25 0.317196259449922 1102 26-27 0.33839572057153333 1102 28-29 0.3837038593646156 1102 30-31 0.33687180755594426 1102 32-33 0.33806204641695814 1102 34-35 0.3843122505940926 1102 36-37 0.4507723087927147 1102 38-39 0.5186598787992338 1102 40-41 0.4823340838090928 1102 42-43 0.3253007854958838 1102 44-45 0.37127974769384053 1102 46-47 0.5545712633052062 1102 48-49 0.6594079658900327 1102 50-51 0.4695806128309954 1102 52-53 0.4765264248316612 1102 54-55 0.655047169106787 1102 56-57 0.6170343194837571 1102 58-59 0.610520705469348 1102 60-61 0.6068267993671697 1102 62-63 0.6078376073918719 1102 64-65 0.6112129026074982 1102 66-67 0.5595695552952975 1102 68-69 0.6381416964073523 1102 70-71 0.5423730211134803 1102 72-73 0.36808836569547054 1102 74-75 0.30740464919959365 1102 76-77 0.4319031368128563 1102 78-79 0.594682885960836 1102 80-81 0.4663164823778807 1102 82-83 0.5465296726716602 1102 84-85 0.4921006536535302 1102 86-87 0.48081445746662865 1102 88-89 0.6836549081425609 1102 90-91 0.6610874072652635 1102 92-93 0.5124712800525657 1102 94-95 0.2831277087825228 1102 96-97 0.5391196475552107 1102 98-99 0.7312506675541215 1102 100-101 0.8500926188186284 1103 1 0.0684308134924585 1103 2 -0.09250081401868471 1103 3 0.04844310099437621 1103 4 -0.10764845952621016 1103 5 -0.12462665856050847 1103 6 -0.022166651244759805 1103 7 -0.02651111234796133 1103 8 -0.054974946281859616 1103 9 0.11307014373542046 1103 10-11 0.05048404059169087 1103 12-13 0.17277207114911874 1103 14-15 0.10857004770976175 1103 16-17 0.04036809549419473 1103 18-19 0.07435057609959728 1103 20-21 0.1731843247493643 1103 22-23 0.07937162990802449 1103 24-25 0.11114339661924788 1103 26-27 0.03348175412654797 1103 28-29 -0.10890569823578744 1103 30-31 -0.06450191018827667 1103 32-33 -0.05490951358130047 1103 34-35 0.05710900240686101 1103 36-37 0.09277310321832744 1103 38-39 0.0794658138546751 1103 40-41 0.04105429972700847 1103 42-43 0.03401403995226815 1103 44-45 0.0342745313325068 1103 46-47 0.021172947799740882 1103 48-49 0.015535862782606102 1103 50-51 -0.06550576111901307 1103 52-53 -0.05027846523675095 1103 54-55 -0.0705736070680203 1103 56-57 0.021139003229301068 1103 58-59 0.009080789856596994 1103 60-61 -0.009846847956232807 1103 62-63 0.06851699484980145 1103 64-65 0.01440290277513867 1103 66-67 0.09134693159467133 1103 68-69 0.15673973995513535 1103 70-71 0.11255235936769381 1103 72-73 0.16499578759881572 1103 74-75 0.2704761752735294 1103 76-77 0.21373022869685343 1103 78-79 0.36054522868970906 1103 80-81 0.3287823804940757 1103 82-83 0.3259426794203115 1103 84-85 0.3199431661985557 1103 86-87 0.4251773756038091 1103 88-89 0.44181066342210684 1103 90-91 0.47582669605215244 1103 92-93 0.6133431455233413 1103 94-95 0.640475972915219 1103 96-97 0.6904456652114739 1103 98-99 0.7960882038058728 1103 100-101 1.1522589834167292 1104 1 -0.11951933749978849 1104 2 -0.01835622273677373 1104 3 0.00786285560343103 1104 4 -0.13048792814885246 1104 5 0.025604361216849725 1104 6 -0.050169417924962545 1104 7 -0.05996134536657394 1104 8 -0.01944209145936071 1104 9 -0.015312592818737869 1104 10-11 -0.03599021758027021 1104 12-13 -0.03920245964987856 1104 14-15 0.07437294470389588 1104 16-17 0.01618755308798825 1104 18-19 -0.010727129877693642 1104 20-21 -0.025897060439824315 1104 22-23 -0.06208915966517736 1104 24-25 -0.12225415511506554 1104 26-27 -0.028156586164847397 1104 28-29 -0.12140736985941203 1104 30-31 -0.04794400245398123 1104 32-33 -0.17448207423765183 1104 34-35 -0.044515719837519896 1104 36-37 -0.09267662062418225 1104 38-39 0.0062904703294535125 1104 40-41 -0.1160479981931104 1104 42-43 -0.20038543394035457 1104 44-45 -0.018737797870258532 1104 46-47 0.14394741416580814 1104 48-49 0.14390501594441218 1104 50-51 -0.062242931264592016 1104 52-53 0.028854015785640286 1104 54-55 0.15122731683781865 1104 56-57 0.09509741849358733 1104 58-59 -0.08733038506105117 1104 60-61 0.09174554024487236 1104 62-63 0.1646552951955016 1104 64-65 0.11457918364727249 1104 66-67 0.14859669131714526 1104 68-69 0.13128180171854353 1104 70-71 0.13161106555173774 1104 72-73 0.2002663808415619 1104 74-75 0.17632965199385353 1104 76-77 0.03723341466760388 1104 78-79 0.06111026686956578 1104 80-81 0.1483444643015659 1104 82-83 0.13179618089397138 1104 84-85 0.34030395966422233 1104 86-87 0.36652506100303484 1104 88-89 0.25012668850193975 1104 90-91 0.32902138307068185 1104 92-93 0.295627784241308 1104 94-95 0.17255329317367796 1104 96-97 0.3861407323483981 1104 98-99 0.3780869972045764 1104 100-101 0.4571030129650353 1105 1 0.054486890781927144 1105 2 0.18345858715505514 1105 3 0.11872747276032314 1105 4 0.11383838570844063 1105 5 0.13135704020082528 1105 6 0.04391725899557741 1105 7 0.12248439452671533 1105 8 0.08172488852025594 1105 9 0.11876072874161991 1105 10-11 0.14506982457456274 1105 12-13 0.15777862764100803 1105 14-15 0.22058571901096258 1105 16-17 0.21677433444617833 1105 18-19 0.20578908327639311 1105 20-21 0.24613760653066663 1105 22-23 0.22978160348921506 1105 24-25 0.2413891365065055 1105 26-27 0.1978573768325731 1105 28-29 0.19772101882587378 1105 30-31 0.22060035822935475 1105 32-33 0.19988813508071956 1105 34-35 0.2673868176809151 1105 36-37 0.2281343852053297 1105 38-39 0.27290694362822165 1105 40-41 0.2651465469967462 1105 42-43 0.1898293911424105 1105 44-45 0.13226258823257098 1105 46-47 0.02086077522259444 1105 48-49 0.07364344710101278 1105 50-51 -0.006059431147491523 1105 52-53 0.022592314021594007 1105 54-55 0.21044631309705153 1105 56-57 0.23648890777448628 1105 58-59 0.2641324383000736 1105 60-61 0.29502073661929984 1105 62-63 0.3490521202909207 1105 64-65 0.20620622730221072 1105 66-67 0.2973280270430507 1105 68-69 0.3901085554798769 1105 70-71 0.18294356338386564 1105 72-73 0.236255405393905 1105 74-75 0.3102921114055839 1105 76-77 0.2751218119610854 1105 78-79 0.5772200909712417 1105 80-81 0.49831259499421066 1105 82-83 0.4593541121423499 1105 84-85 0.46596938573818747 1105 86-87 0.37037153132691714 1105 88-89 0.47491673717621197 1105 90-91 0.5500927867760055 1105 92-93 0.4714770522283196 1105 94-95 0.5870449349968396 1105 96-97 0.6866855171118935 1105 98-99 0.7946148250913296 1105 100-101 0.9939725418562873 1106 1 -0.021457421584596403 1106 2 0.07236968304362534 1106 3 -0.07049778949270191 1106 4 -8.522161639277215E-4 1106 5 -0.013789969241628341 1106 6 -0.08088213926636456 1106 7 0.047082049360092526 1106 8 0.037602848520798204 1106 9 -0.04764388637435957 1106 10-11 -0.16079853179405745 1106 12-13 -0.28016854566761396 1106 14-15 -0.34793035196188526 1106 16-17 -0.41361739971453915 1106 18-19 -0.27607308110017925 1106 20-21 -0.3196790379737777 1106 22-23 -0.1404831343920776 1106 24-25 -0.2594404023848469 1106 26-27 -0.04046689013495808 1106 28-29 0.20411146354791043 1106 30-31 0.14608161435755562 1106 32-33 0.2290633644261959 1106 34-35 -0.012767646328974536 1106 36-37 -0.13619571109962436 1106 38-39 -0.24092403245000327 1106 40-41 0.03273657265120278 1106 42-43 0.07565228136550672 1106 44-45 0.021842983391799464 1106 46-47 0.08483594009613427 1106 48-49 0.17218134451004374 1106 50-51 0.0066620232318967965 1106 52-53 0.0013604971218867945 1106 54-55 0.10987613942577923 1106 56-57 -0.0331710027707075 1106 58-59 -0.00107675969592691 1106 60-61 -0.027556596123041288 1106 62-63 0.20360706246765403 1106 64-65 0.1266863417398909 1106 66-67 0.18508326202982062 1106 68-69 0.058141624209646636 1106 70-71 0.05457416606213883 1106 72-73 0.30063224385141396 1106 74-75 0.2716982040034033 1106 76-77 0.2177753693343938 1106 78-79 0.23769711612872513 1106 80-81 0.33878438180700954 1106 82-83 0.2560566413226155 1106 84-85 0.32253551648189926 1106 86-87 0.17644129533277564 1106 88-89 0.45808761466610903 1106 90-91 0.5222435973762636 1106 92-93 0.4842606280029891 1106 94-95 0.47539996044982047 1106 96-97 0.6140816568548111 1106 98-99 0.6473792607604238 1106 100-101 0.7899414341478597 1107 1 -0.05034620065493911 1107 2 -0.12117614917779562 1107 3 -0.04925027276459204 1107 4 -0.07225256736364116 1107 5 -0.08487905075224234 1107 6 -0.006503640859278903 1107 7 -0.1454111061989778 1107 8 0.02517433215837883 1107 9 -0.0491976650658259 1107 10-11 -0.04275068824595252 1107 12-13 -0.04842117884181363 1107 14-15 -0.07701950595274809 1107 16-17 -0.1187336481372725 1107 18-19 0.02234447756763558 1107 20-21 -0.009060748573347155 1107 22-23 0.02269403696768535 1107 24-25 0.01205193282967798 1107 26-27 -0.03383106051624196 1107 28-29 -0.05942702181273063 1107 30-31 -0.012029056381187786 1107 32-33 -0.046877709137952195 1107 34-35 0.04777868995957846 1107 36-37 -0.06721636467371894 1107 38-39 -0.05243896832146078 1107 40-41 -0.08476878923934805 1107 42-43 0.08213224552072518 1107 44-45 0.07466220119822253 1107 46-47 -0.020085039968506635 1107 48-49 0.010085150052155711 1107 50-51 -0.0444266299863898 1107 52-53 -0.17569505898276105 1107 54-55 -0.20931662836400022 1107 56-57 -0.13454157118052024 1107 58-59 -0.10013461540093971 1107 60-61 -0.1947107583008787 1107 62-63 -0.19077855218125706 1107 64-65 -0.12742463868715248 1107 66-67 -0.2543047741180544 1107 68-69 -0.36063164771315215 1107 70-71 -0.1663597747873986 1107 72-73 -0.1579148897197875 1107 74-75 -0.3273106945934465 1107 76-77 -0.3709691411109972 1107 78-79 -0.14129224574864452 1107 80-81 -0.1368035153105822 1107 82-83 -0.07094583947835886 1107 84-85 -0.0822671890078226 1107 86-87 -0.0020232437093667954 1107 88-89 -0.02720469485087662 1107 90-91 0.21321325121721912 1107 92-93 0.11298164826121848 1107 94-95 0.08198446087975952 1107 96-97 0.22024623312544023 1107 98-99 0.4193729286230159 1107 100-101 0.630721453322181 1108 1 -0.16095885930853626 1108 2 -0.24562883610914454 1108 3 -0.29173024398855674 1108 4 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-0.7544351863926693 2316 68-69 -0.31418350972286646 2316 70-71 -0.5684988004402847 2316 72-73 -0.6702635222633795 2316 74-75 -1.046661276712424 2316 76-77 -0.7209152799604155 2316 78-79 -0.6866074271032119 2316 80-81 -0.8095779703657087 2316 82-83 -0.5660646314024902 2316 84-85 -0.6849064955317381 2316 86-87 -0.8752030342126176 2316 88-89 -0.7655051497518031 2316 90-91 -0.9459937011668771 2316 92-93 -0.7985283364285571 2316 94-95 -0.7557194459811996 2316 96-97 -0.9387880111111961 2316 98-99 -1.2292126929831255 2316 100-101 -1.411213142278612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14207.0 3 3105.0 4 2939.0 5 3441.0 6 3827.0 7 4586.0 8 5648.0 9 6994.0 10 8956.0 11 11427.0 12 14501.0 13 17817.0 14 19970.0 15 23509.0 16 27775.0 17 34034.0 18 29783.0 19 22688.0 20 22686.0 21 24180.0 22 26106.0 23 28886.0 24 32193.0 25 36880.0 26 42424.0 27 49417.0 28 58522.0 29 70244.0 30 84645.0 31 103955.0 32 129918.0 33 166989.0 34 221073.0 35 305099.0 36 433689.0 37 594977.0 38 488450.0 39 67521.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70943233705455 19.288919196038236 15.156892095341288 22.844756371565925 2 14.97156995399544 20.287903506398113 40.72732052422005 24.013206015386398 3 18.394363444160525 25.02766504542387 30.26911640721215 26.308855103203456 4 12.439365054229693 17.00497079749258 37.97145564695214 32.58420850132559 5 13.45865487048579 37.525059411252656 34.49891227543938 14.517373442822176 6 31.055809068174 36.722343991369826 17.99546798340094 14.226378957055234 7 27.759199081521174 33.03164456229323 21.411187702520408 17.797968653665187 8 26.094870603883567 34.618193303189685 20.395112364984787 18.891823727941965 9 26.18968007579836 16.8550912652034 20.792879008786898 36.16234965021135 10-11 24.20561918891559 26.938197390008067 27.988563765798265 20.867619655278077 12-13 25.363846181423042 24.326051670917153 28.375629074069725 21.934473073590077 14-15 22.43337278179644 25.615735919834133 26.730036554202496 25.22085474416693 16-17 21.656366590513834 28.89489286710391 27.283059072398242 22.165681469984015 18-19 21.70400018045416 27.274657098476368 29.839068263273965 21.18227445779551 20-21 22.297624780779717 26.46782763225408 29.97105697432981 21.26349061263639 22-23 22.036311678744603 26.32830007008211 29.873394878691208 21.76199337248208 24-25 21.78303968756059 26.604361081951232 29.904301526278264 21.708297704209915 26-27 21.64898850682693 26.875999688505097 29.659908133725253 21.81510367094272 28-29 21.642812323325124 27.001290330156717 29.73209000574321 21.623807340774942 30-31 21.775293420646392 26.677489629024574 29.72820828052426 21.81900866980477 32-33 21.530734416761238 26.74998516529569 29.62122673114818 22.098053686794888 34-35 22.072897158428894 26.768996249937615 29.458608788813752 21.69949780281974 36-37 22.02694129974436 26.624773153625785 29.40403328450066 21.944252262129194 38-39 21.753941042175896 26.879713593347415 29.370817455925614 21.995527908551075 40-41 22.09227129995449 26.775710709122542 29.330336494031712 21.801681496891263 42-43 21.838957047158615 26.800345928923903 29.21483465332799 22.14586237058949 44-45 21.93765165571264 26.989942360013202 29.118793009034754 21.953612975239402 46-47 22.124449866342587 27.249704056442948 28.542693894400912 22.08315218281355 48-49 22.06889486719472 27.32841681114983 28.37391815317837 22.228770168477077 50-51 22.22670863601174 27.42749239344252 28.263943295026262 22.081855675519478 52-53 22.3583366045294 27.37175108413104 28.163808104942028 22.106104206397532 54-55 22.161634406049842 27.385285969135136 28.241947826688968 22.21113179812605 56-57 22.169657646791492 27.6793502884598 28.090965671067003 22.060026393681706 58-59 22.2666301120194 27.283987133793868 28.093168013653937 22.3562147405328 60-61 22.07415561148752 27.339305359592863 28.401597346077335 22.184941682842275 62-63 21.99301418726748 27.40079385920739 28.523862426572038 22.08232952695309 64-65 21.96755608191009 27.324267259242873 28.394120433823367 22.314056225023666 66-67 21.876675694335123 27.507255838541973 28.259094651172095 22.35697381595081 68-69 22.08212383978211 27.365849710154063 28.309285573939448 22.24274087612438 70-71 22.004490034443783 27.3352512193272 28.25060589821775 22.40965284801127 72-73 22.04873725829245 27.342437122472575 28.118268198449975 22.490557420785 74-75 22.028368656279028 27.721255606323297 27.941716019613576 22.3086597177841 76-77 22.145638573933226 27.437382928060742 27.99585199943886 22.421126498567173 78-79 22.12467176759446 27.5486253636126 27.97457817230556 22.35212469648738 80-81 22.080299807368206 27.636298194636893 27.880478690783487 22.402923307211413 82-83 22.15964955322795 27.626635882788076 27.866244006320745 22.347470557663225 84-85 22.12867734400511 27.570869976635404 27.8649277452317 22.435524934127784 86-87 22.17592237155061 27.4532969244009 27.910406467719255 22.460374236329233 88-89 22.167493544666396 27.44646870001307 27.771684034863895 22.614353720456634 90-91 22.12857853047017 27.359875101921517 27.91291615570734 22.59863021190097 92-93 22.18184351828335 27.189039692353518 28.02679609274629 22.60232069661684 94-95 21.998744307827497 27.119057785940328 28.059467406074905 22.82273050015727 96-97 21.997723603158036 27.103102733838913 28.013785053514482 22.885388609488572 98-99 21.907276738725294 27.228395117993966 27.86171923845996 23.002608904820782 100-101 22.053972663806086 26.967008398696258 28.20417607033447 22.774842867163187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4530.0 1 2603.0 2 665.0 3 627.5 4 559.5 5 554.0 6 827.0 7 1517.0 8 2525.5 9 3202.0 10 3124.0 11 3014.5 12 3245.5 13 3480.5 14 3742.5 15 4028.5 16 4202.0 17 4480.0 18 4871.5 19 5341.5 20 5947.5 21 7038.5 22 8524.0 23 10295.0 24 12550.0 25 16041.5 26 20620.0 27 26043.5 28 30908.0 29 35914.5 30 42673.0 31 49710.0 32 56737.5 33 62256.0 34 67381.0 35 73460.0 36 80726.5 37 88073.0 38 93420.5 39 96505.0 40 99079.0 41 104563.0 42 108932.0 43 112319.5 44 117647.5 45 122363.0 46 123486.5 47 123027.5 48 122751.0 49 120696.5 50 116698.0 51 111447.0 52 106206.0 53 103731.0 54 102146.0 55 94551.0 56 84238.5 57 75343.0 58 68874.5 59 61452.5 60 50635.5 61 40884.5 62 33120.5 63 25276.5 64 18659.5 65 14173.0 66 10353.0 67 7758.0 68 5859.5 69 4257.0 70 3217.5 71 2349.0 72 1800.0 73 1535.0 74 1199.5 75 887.5 76 630.0 77 407.0 78 265.0 79 170.0 80 96.5 81 60.5 82 46.5 83 36.0 84 24.0 85 15.5 86 14.5 87 14.0 88 13.5 89 10.0 90 7.0 91 9.0 92 10.0 93 8.0 94 3.5 95 1.0 96 2.5 97 3.5 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33841401462626913 2 0.2989143925090709 3 0.3005486469760588 4 0.30172037659389916 5 0.28534699693407695 6 0.21528989978319918 7 0.22460206674603592 8 0.22293697728910486 9 0.21939095344564052 10-11 0.227824323195097 12-13 0.22697636097165985 14-15 0.20903039682473606 16-17 0.22359992957288297 18-19 0.2093695817141109 20-21 0.21225265327379714 22-23 0.21168220595984855 24-25 0.20355718610982373 26-27 0.21772586398870947 28-29 0.2181729713428854 30-31 0.22682218602194404 32-33 0.2285797804487046 34-35 0.21552116220777293 36-37 0.21115801113081467 38-39 0.2020308541076369 40-41 0.20190751414786426 42-43 0.20246254396684127 44-45 0.21957596338529956 46-47 0.2099862815129743 48-49 0.21922136100095307 50-51 0.22737721584092105 52-53 0.23924868696904075 54-55 0.2326499991212028 56-57 0.19455336904641868 58-59 0.18212686809932196 60-61 0.19117693764764176 62-63 0.19188614241633467 64-65 0.18900307085664844 66-67 0.201583746753461 68-69 0.19046773287894891 70-71 0.19566342868437273 72-73 0.20960084413868466 74-75 0.1983769077993715 76-77 0.21001711650291743 78-79 0.20952375666382678 80-81 0.19500047640059462 82-83 0.20792033718678204 84-85 0.18871013845218834 86-87 0.19480004896596403 88-89 0.20344926364502264 90-91 0.20073578453002386 92-93 0.20434347835337455 94-95 0.20372677855451118 96-97 0.19657306088769622 98-99 0.20516060558686847 100-101 0.18743048636954687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3243069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.00002193930104 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05458861083368 38.50840232469264 2 13.895332722195647 14.451152128161226 3 4.985544453309272 7.777452628543284 4 2.3017165809419144 4.787572508281301 5 1.2832353578292104 3.3364133380202885 6 0.7550260691487785 2.3556823296288427 7 0.5091480941110621 1.853299844488998 8 0.37068512651675706 1.5420507769155158 9 0.2630256829357866 1.230960715493449 >10 1.420924727645272 13.526467547975656 >50 0.10858654808885052 3.864726087603538 >100 0.048621435457429765 4.357267400473817 >500 0.0026734432402272627 0.9542889375576312 >1k 7.129181968470598E-4 0.6215698741372649 >5k 1.1881969947450997E-4 0.45660053378473037 >10k+ 5.9409849737254986E-5 0.3760930242419018 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12174 0.3753851675681276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8722 0.26894278228431157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6058 0.18679836907571193 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4903 0.15118395569135287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.083498994316803E-5 6 3.083498994316803E-5 0.0 0.0 0.0 6.166997988633606E-5 7 3.083498994316803E-5 0.0 0.0 0.0 6.166997988633606E-5 8 3.083498994316803E-5 0.0 0.0 0.0 6.166997988633606E-5 9 3.083498994316803E-5 0.0 0.0 0.0 6.166997988633606E-5 10-11 3.083498994316803E-5 0.0 0.0 3.083498994316803E-5 6.166997988633606E-5 12-13 6.166997988633606E-5 0.0 0.0 3.083498994316803E-5 1.6959244468742418E-4 14-15 6.166997988633606E-5 0.0 0.0 3.083498994316803E-5 2.4667991954534425E-4 16-17 7.708747485792008E-5 0.0 0.0 3.083498994316803E-5 2.7751490948851225E-4 18-19 1.0792246480108811E-4 0.0 0.0 4.6252484914752047E-5 2.7751490948851225E-4 20-21 1.2333995977267212E-4 0.0 0.0 6.166997988633606E-5 2.7751490948851225E-4 22-23 1.2333995977267212E-4 0.0 0.0 1.2333995977267212E-4 3.237673944032643E-4 24-25 1.5417494971584016E-4 0.0 0.0 2.1584492960217622E-4 3.391848893748483E-4 26-27 1.5417494971584016E-4 0.0 0.0 5.241948290338565E-4 4.008548692611844E-4 28-29 2.1584492960217622E-4 0.0 0.0 0.001079224648010881 4.008548692611844E-4 30-31 2.4667991954534425E-4 0.0 0.0 0.002158449296021762 4.008548692611844E-4 32-33 2.4667991954534425E-4 0.0 0.0 0.003885208732839172 4.008548692611844E-4 34-35 2.6209741451692825E-4 0.0 0.0 0.005920318069088262 4.008548692611844E-4 36-37 3.083498994316803E-4 0.0 0.0 0.008726302153916553 4.008548692611844E-4 38-39 3.083498994316803E-4 0.0 0.0 0.013582813069965517 4.008548692611844E-4 40-41 3.083498994316803E-4 0.0 0.0 0.018408488996071313 4.008548692611844E-4 42-43 3.083498994316803E-4 0.0 0.0 0.024020457165727895 4.008548692611844E-4 44-45 3.083498994316803E-4 0.0 0.0 0.028614870667259932 4.162723642327684E-4 46-47 3.5460238434643237E-4 0.0 0.0 0.03479728615086512 4.4710735417593644E-4 48-49 4.3168985920435243E-4 0.0 0.0 0.04107220660429982 4.779423441191045E-4 50-51 4.3168985920435243E-4 0.0 0.0 0.047208369602990255 4.933598390906885E-4 52-53 4.3168985920435243E-4 0.0 0.0 0.06171623237125081 4.933598390906885E-4 54-55 4.6252484914752044E-4 0.0 0.0 0.08576752452692188 4.933598390906885E-4 56-57 5.241948290338565E-4 0.0 0.0 0.12059564566773016 4.933598390906885E-4 58-59 5.241948290338565E-4 0.0 0.0 0.16724898545174338 4.933598390906885E-4 60-61 5.241948290338565E-4 0.0 0.0 0.22765473075040957 4.933598390906885E-4 62-63 5.396123240054405E-4 0.0 0.0 0.26673808050337505 4.933598390906885E-4 64-65 5.550298189770245E-4 0.0 0.0 0.31832501867829516 5.241948290338565E-4 66-67 6.166997988633606E-4 0.0 0.0 0.4078081594933688 5.241948290338565E-4 68-69 6.166997988633606E-4 0.0 0.0 0.525767413520958 5.241948290338565E-4 70-71 6.166997988633606E-4 0.0 0.0 0.646039291794285 5.241948290338565E-4 72-73 6.166997988633606E-4 0.0 0.0 0.7848584165184275 5.396123240054405E-4 74-75 6.166997988633606E-4 0.0 0.0 0.9219661993007241 5.550298189770245E-4 76-77 6.166997988633606E-4 0.0 0.0 1.0610165864494403 5.550298189770245E-4 78-79 6.321172938349446E-4 0.0 0.0 1.1971376495535555 5.550298189770245E-4 80-81 6.475347888065286E-4 0.0 0.0 1.3400578279401394 5.858648089201925E-4 82-83 6.475347888065286E-4 0.0 0.0 1.5329152725396837 6.166997988633606E-4 84-85 6.937872737212806E-4 0.0 0.0 1.801842637329024 6.321172938349446E-4 86-87 7.708747485792007E-4 0.0 0.0 2.1083578548590856 6.629522837781126E-4 88-89 7.708747485792007E-4 0.0 0.0 2.4762501198710236 6.783697787496966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6205 0.0 49.659912 1 GTATCAA 11565 0.0 44.160236 1 ATCAACG 14800 0.0 34.202072 3 TATCAAC 15175 0.0 33.576126 2 TCAACGC 15085 0.0 33.559006 4 CAACGCA 15390 0.0 32.831146 5 AACGCAG 15915 0.0 31.728981 6 ACGCAGA 18320 0.0 27.434034 7 CGCAGAG 18475 0.0 27.07489 8 GCAGAGT 20070 0.0 24.44869 9 GTACATG 12345 0.0 23.843626 1 TACATGG 12190 0.0 23.433397 2 ACATGGG 12550 0.0 22.117376 3 AGAGTAC 18530 0.0 21.366638 10-11 GAGTACT 11880 0.0 21.050924 12-13 AGTACTT 12405 0.0 20.868391 12-13 CAGAGTA 19665 0.0 19.976416 10-11 GTACTTT 12650 0.0 19.52489 14-15 TGGTATC 3290 0.0 19.503044 2 GTGGTAT 3720 0.0 18.918709 1 >>END_MODULE