FastQCFastQC Report
Thu 26 May 2016
SRR937521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243069
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140140.43212154906355676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111430.34359429293672133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.23391423370887268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70450.052.485521
GTATCAA126600.041.2576871
ATCAACG165350.031.402383
TATCAAC167450.031.0369322
TCAACGC167450.030.9801924
CAACGCA170700.030.4460125
AACGCAG176650.029.4150756
ACGCAGA202250.025.624547
CGCAGAG203100.025.4237428
GTACATG119900.023.8626231
GCAGAGT220650.023.2078369
TACATGG122400.023.0542852
ACATGGG122400.022.161613
TGGTATC33350.022.0791572
GAGTACT136950.021.81767512-13
AGAGTAC204750.020.88038310-11
AGTACTT143700.020.7432512-13
GTACTTT145500.020.32088514-15
CAGAGTA217000.019.77826710-11
CATGGGG63950.019.7600354
Produced by FastQC (version 0.11.5)