##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3243069 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.029767174241435 31.0 31.0 34.0 30.0 34.0 2 32.25308280520704 33.0 31.0 34.0 30.0 34.0 3 32.33177030769311 34.0 31.0 34.0 30.0 34.0 4 35.977805282588804 37.0 35.0 37.0 35.0 37.0 5 35.849046690033425 37.0 35.0 37.0 35.0 37.0 6 35.92233621918004 37.0 35.0 37.0 35.0 37.0 7 35.87182048855575 37.0 35.0 37.0 35.0 37.0 8 35.84624903139588 37.0 35.0 37.0 35.0 37.0 9 37.524472960643145 39.0 37.0 39.0 35.0 39.0 10-11 37.44028557517586 39.0 37.0 39.0 34.0 39.0 12-13 37.35739896375932 39.0 37.0 39.0 34.0 39.0 14-15 38.54241753721552 40.0 38.0 41.0 34.0 41.0 16-17 38.51544709656193 40.0 38.0 41.0 34.0 41.0 18-19 38.51329620183844 40.0 38.0 41.0 34.0 41.0 20-21 38.40037075991907 40.0 38.0 41.0 34.0 41.0 22-23 38.27452638226322 40.0 38.0 41.0 34.0 41.0 24-25 38.12916746452203 40.0 37.5 41.0 33.0 41.0 26-27 38.06648779289001 40.0 37.0 41.0 33.0 41.0 28-29 37.96065239438322 40.0 37.0 41.0 33.0 41.0 30-31 38.0305203188708 40.0 37.0 41.0 33.0 41.0 32-33 37.95267245316088 40.0 37.0 41.0 33.0 41.0 34-35 37.83537275956817 40.0 37.0 41.0 33.0 41.0 36-37 37.66016665078665 40.0 37.0 41.0 33.0 41.0 38-39 37.520833969305 40.0 37.0 41.0 33.0 41.0 40-41 37.347376975328 40.0 36.5 41.0 32.0 41.0 42-43 37.130312984398415 40.0 36.0 41.0 31.5 41.0 44-45 36.8455051372635 39.5 36.0 41.0 31.0 41.0 46-47 36.510301970140006 39.0 36.0 41.0 30.0 41.0 48-49 36.21302152374803 39.0 35.0 41.0 30.0 41.0 50-51 35.9886858713151 39.0 35.0 41.0 29.0 41.0 52-53 35.742090285467256 39.0 35.0 40.0 28.5 41.0 54-55 35.50586373586255 39.0 35.0 40.0 28.0 41.0 56-57 35.2488496236127 38.0 34.5 40.0 27.5 41.0 58-59 34.99737717575543 38.0 34.0 40.0 27.0 41.0 60-61 34.666455909510404 38.0 34.0 40.0 26.0 41.0 62-63 34.294658701372065 37.0 34.0 40.0 26.0 41.0 64-65 33.93846939426821 37.0 33.0 39.5 25.5 41.0 66-67 33.51082894011814 36.0 33.0 39.0 24.5 40.5 68-69 33.104815377039465 36.0 32.0 39.0 23.0 40.0 70-71 32.698592906903926 35.0 32.0 38.5 22.5 40.0 72-73 32.249738904722655 35.0 31.5 37.5 22.0 40.0 74-75 31.799415615270597 35.0 31.0 37.0 20.5 39.0 76-77 30.479715972740635 33.5 29.5 35.5 19.5 38.5 78-79 31.13070227614645 34.0 31.0 36.0 20.0 38.5 80-81 30.99691156740729 34.0 31.0 36.0 19.5 37.5 82-83 30.659729873154102 34.0 31.0 35.0 18.0 37.0 84-85 30.28051099128634 34.0 30.5 35.0 16.0 36.5 86-87 29.89280986621006 34.0 30.0 35.0 10.0 36.0 88-89 29.48079889758744 34.0 29.5 35.0 7.0 36.0 90-91 29.019595019409085 34.0 29.0 35.0 2.0 35.0 92-93 28.624966197142275 34.0 29.0 35.0 2.0 35.0 94-95 28.179152216619507 33.0 29.0 35.0 2.0 35.0 96-97 27.663643295902737 33.0 27.0 35.0 2.0 35.0 98-99 27.113993874320897 33.0 27.0 35.0 2.0 35.0 100-101 25.165913059512455 31.5 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13182697977052982 1101 2 0.13803395093349025 1101 3 0.14059730958615546 1101 4 0.06931591750791455 1101 5 -0.06793121639032762 1101 6 0.005433473067554928 1101 7 0.051054494618021806 1101 8 0.034097335401256146 1101 9 0.1404831785488696 1101 10-11 0.05442664863036839 1101 12-13 0.09037326816224578 1101 14-15 0.11156065973796103 1101 16-17 0.15235975131506763 1101 18-19 0.05262360696218593 1101 20-21 0.14314778676760653 1101 22-23 0.10323255291468314 1101 24-25 0.14496096924303714 1101 26-27 0.029704616163151343 1101 28-29 0.018741057214128887 1101 30-31 0.14820366338268087 1101 32-33 0.12087074654189678 1101 34-35 0.06864844525131986 1101 36-37 0.01832810615508862 1101 38-39 -0.006947450433052893 1101 40-41 0.029692154438961893 1101 42-43 0.17292684705974182 1101 44-45 0.240192285703408 1101 46-47 0.26724071137886796 1101 48-49 0.262906208246811 1101 50-51 0.07093203220868816 1101 52-53 0.17328306597491405 1101 54-55 0.21868064067869142 1101 56-57 0.1292222149427289 1101 58-59 0.0015757224662351632 1101 60-61 0.05807796269414922 1101 62-63 0.03439607472454753 1101 64-65 0.04905739767227146 1101 66-67 0.1355202803294162 1101 68-69 0.12055446168651685 1101 70-71 -0.04111771099246653 1101 72-73 -0.014411466120499483 1101 74-75 -0.07481539474308008 1101 76-77 -0.168914403884461 1101 78-79 -0.07250985464863646 1101 80-81 0.09927255871482288 1101 82-83 0.010613546663005735 1101 84-85 -0.02841304226162933 1101 86-87 0.004634057889695953 1101 88-89 -0.10523288362695027 1101 90-91 -0.0871946434745432 1101 92-93 -0.10511308306979217 1101 94-95 -0.1529754840774551 1101 96-97 -0.14806882140881328 1101 98-99 -0.073817805185044 1101 100-101 0.3916279245812042 1102 1 0.07555491943167425 1102 2 0.09400775662771821 1102 3 0.1295509750009316 1102 4 0.08951557506739505 1102 5 0.025125784054878864 1102 6 0.10117706554756012 1102 7 0.10122632336887705 1102 8 0.12380201355384202 1102 9 0.08875929425219908 1102 10-11 0.03502772628957729 1102 12-13 0.035372387520673954 1102 14-15 0.0417909786024282 1102 16-17 0.04543408374929925 1102 18-19 0.06907696044128642 1102 20-21 0.08060548487886621 1102 22-23 0.1117666183640651 1102 24-25 0.09205072634510714 1102 26-27 0.031776330477406134 1102 28-29 0.05895575187891211 1102 30-31 0.07812388541402271 1102 32-33 0.039745035191472766 1102 34-35 0.12071973830195049 1102 36-37 0.13069957561488366 1102 38-39 0.010064612283606778 1102 40-41 0.21102661863108807 1102 42-43 0.16185978362960896 1102 44-45 0.07586212762792144 1102 46-47 0.14264550894297656 1102 48-49 0.09456461747780764 1102 50-51 0.17320434156115283 1102 52-53 0.23043071940949034 1102 54-55 0.1724210470683616 1102 56-57 0.15493801873444824 1102 58-59 0.2337819148275102 1102 60-61 0.21040468192114758 1102 62-63 0.060010842114145646 1102 64-65 0.11011825582237122 1102 66-67 0.10299986542619877 1102 68-69 0.14368230262486037 1102 70-71 -0.06914805748647979 1102 72-73 0.09160397111837426 1102 74-75 0.07841533615733098 1102 76-77 0.08347773825175508 1102 78-79 0.0657691938519136 1102 80-81 0.15783923531795807 1102 82-83 0.2273461578033853 1102 84-85 0.0974982746579407 1102 86-87 0.14926629675339242 1102 88-89 0.08611712318682052 1102 90-91 0.1846268455974105 1102 92-93 0.16340546516749654 1102 94-95 0.2036062183972902 1102 96-97 0.19203632465398357 1102 98-99 0.24626020080171074 1102 100-101 0.5661662698015242 1103 1 0.05645754458090124 1103 2 0.08041243176100465 1103 3 0.0717046848392826 1103 4 0.02146767253400128 1103 5 -0.09613814094765871 1103 6 -0.03998294684486581 1103 7 0.007835629640041475 1103 8 -0.06261261749317981 1103 9 -0.051672771928778616 1103 10-11 -0.07065583356639138 1103 12-13 -0.02877213779530763 1103 14-15 -0.02188182451579479 1103 16-17 9.836162756400313E-4 1103 18-19 0.023636340236485864 1103 20-21 -0.002523867394252477 1103 22-23 -0.006148510926529127 1103 24-25 -0.07001760065573137 1103 26-27 0.004051446157312455 1103 28-29 -0.10831869900701463 1103 30-31 -0.11847396259859266 1103 32-33 -0.0677265901344839 1103 34-35 -0.04189158909647972 1103 36-37 -0.10530971025817593 1103 38-39 -0.05920164199324773 1103 40-41 -0.04603200263354523 1103 42-43 0.03245254823968935 1103 44-45 -0.0034428996862203576 1103 46-47 0.029623877608933924 1103 48-49 0.1156914055272793 1103 50-51 0.10164992270306783 1103 52-53 0.03964594770895502 1103 54-55 0.04236370202902151 1103 56-57 -0.14967525480196997 1103 58-59 -0.01561058888132294 1103 60-61 -0.09400768178809216 1103 62-63 -0.058905829063398585 1103 64-65 -0.16601755333427093 1103 66-67 -0.04412070644762167 1103 68-69 0.027833537305333778 1103 70-71 -0.0923056023991009 1103 72-73 0.016087949583400984 1103 74-75 -0.02229701714810517 1103 76-77 -0.02186011593659387 1103 78-79 0.07394660772270001 1103 80-81 -0.05242716727385499 1103 82-83 -0.08826420945082347 1103 84-85 0.09230043670361354 1103 86-87 0.12906929251086652 1103 88-89 0.0661629702975155 1103 90-91 0.11331198291543743 1103 92-93 0.10319634107164077 1103 94-95 0.0406683805376602 1103 96-97 0.0240939765606889 1103 98-99 0.08257586136443251 1103 100-101 0.26709286080580696 1104 1 -0.017897450044845442 1104 2 0.024760994851590112 1104 3 0.02476864949795754 1104 4 -0.03673713271945189 1104 5 -0.04340536971717057 1104 6 -0.031885574157605845 1104 7 -0.06006307649697362 1104 8 -0.03307755751987429 1104 9 0.0211480729037703 1104 10-11 -0.04901355997013468 1104 12-13 -0.007647920549892717 1104 14-15 -0.06195762788991033 1104 16-17 -0.11874244599669481 1104 18-19 -0.08996890311385641 1104 20-21 -0.18743195733474494 1104 22-23 -0.044554856592363024 1104 24-25 -0.06998105854557224 1104 26-27 -0.1887346091011679 1104 28-29 -0.21857886954882844 1104 30-31 -0.09330981550164807 1104 32-33 -0.15158374012589348 1104 34-35 -0.0717603859030973 1104 36-37 -0.14708145740855372 1104 38-39 -0.15455588046649638 1104 40-41 -0.08615477545999539 1104 42-43 -0.026204809853005884 1104 44-45 0.032545571200671475 1104 46-47 -0.010913018504858485 1104 48-49 -0.06958006336467548 1104 50-51 -0.17722823018330303 1104 52-53 -0.1001253958383046 1104 54-55 -0.04991054090725555 1104 56-57 -0.032134942913671694 1104 58-59 -0.10845474640436947 1104 60-61 -0.1380330962842251 1104 62-63 -0.14531037565687654 1104 64-65 -0.28777954643317116 1104 66-67 -0.12473352970219054 1104 68-69 0.017969797604962423 1104 70-71 -0.10094662019681522 1104 72-73 -0.0692415287481154 1104 74-75 -0.13899274526094274 1104 76-77 -0.0700911219758531 1104 78-79 -0.030334982745873873 1104 80-81 -0.06516640902599491 1104 82-83 -0.07584094401444474 1104 84-85 -0.015009662366036736 1104 86-87 0.13967117664805784 1104 88-89 0.10123974493059507 1104 90-91 0.10333697697618405 1104 92-93 0.03887294469630831 1104 94-95 -0.13634132258383858 1104 96-97 -0.0814455474289737 1104 98-99 0.06018860522268099 1104 100-101 0.3938822252138152 1105 1 -0.032248512460579803 1105 2 0.01679999756697015 1105 3 0.04366610438405871 1105 4 0.013072727460688327 1105 5 -0.07522093915554962 1105 6 -0.011059947056985209 1105 7 -0.040215935240304646 1105 8 -0.04609707153667131 1105 9 0.03028440518006903 1105 10-11 -0.005642945560268231 1105 12-13 -0.026053379157779943 1105 14-15 0.009583808539211702 1105 16-17 -0.026593245735917037 1105 18-19 0.04983617825256914 1105 20-21 0.01913691771351722 1105 22-23 0.020340262688094413 1105 24-25 -0.04226191354180742 1105 26-27 0.023391361694272916 1105 28-29 -0.02131459798561508 1105 30-31 0.06875450543208927 1105 32-33 0.09658388546829144 1105 34-35 -0.0348822171698302 1105 36-37 -0.041176774171113095 1105 38-39 -0.06853612592662728 1105 40-41 0.04791474836760301 1105 42-43 -0.1640062390664312 1105 44-45 -0.017742929897508475 1105 46-47 -0.16139501408682833 1105 48-49 -0.10120441695891458 1105 50-51 -0.1828167838167829 1105 52-53 -0.17957026225305128 1105 54-55 -0.18633165250363248 1105 56-57 -0.1687172999132045 1105 58-59 -0.16955004417825847 1105 60-61 -0.24263101132893894 1105 62-63 -0.18577841457097577 1105 64-65 -0.271611237247086 1105 66-67 -0.23729247367930384 1105 68-69 -0.23632790666558634 1105 70-71 -0.2205916904232197 1105 72-73 -0.26845669562682417 1105 74-75 -0.147347219306603 1105 76-77 -0.11843100639818616 1105 78-79 -0.11067051243465542 1105 80-81 -0.1276174340973597 1105 82-83 -0.15151099066326168 1105 84-85 -0.1400921364623322 1105 86-87 -0.03495838616960967 1105 88-89 -0.05749519864142627 1105 90-91 -0.12786304327839204 1105 92-93 -0.23191907246944155 1105 94-95 -0.11919348317729828 1105 96-97 0.0341999089002627 1105 98-99 0.09900257328374806 1105 100-101 0.4881329325415429 1106 1 -0.25060971647396 1106 2 -0.07421624130154925 1106 3 -0.18037131620129543 1106 4 -0.0708172135743581 1106 5 -0.15456722060240935 1106 6 -0.3009886249793752 1106 7 -0.21188817604298293 1106 8 -0.18326164958129 1106 9 -0.3303549415043321 1106 10-11 -0.43509278505200655 1106 12-13 -0.4536132507511752 1106 14-15 -0.37695351130874855 1106 16-17 -0.24867945008508485 1106 18-19 -0.2736112569732896 1106 20-21 -0.27815915621430776 1106 22-23 -0.2546450968101013 1106 24-25 -0.15644950000807256 1106 26-27 -0.22555935757075218 1106 28-29 -0.17062105458123256 1106 30-31 -0.15736965676855874 1106 32-33 -0.1863746375871358 1106 34-35 -0.31542720649166966 1106 36-37 -0.2207173444791053 1106 38-39 -0.20063744088545832 1106 40-41 -0.3342069155455718 1106 42-43 -0.5500421553066417 1106 44-45 -0.459147074905637 1106 46-47 -0.24709903418057877 1106 48-49 -0.2285673805188395 1106 50-51 -0.17279180094214297 1106 52-53 -0.05900794609880933 1106 54-55 -0.31555335536859985 1106 56-57 -0.26990009127934655 1106 58-59 -0.3791111649153933 1106 60-61 -0.2967386794596578 1106 62-63 -0.1750862143318912 1106 64-65 -0.1619046517076015 1106 66-67 -0.35547188026887966 1106 68-69 -0.35382921625176067 1106 70-71 -0.26443670257886964 1106 72-73 -0.27821821662721646 1106 74-75 -0.5535239664288731 1106 76-77 -0.4724184612342519 1106 78-79 -0.3811850790623694 1106 80-81 -0.3140497855768558 1106 82-83 -0.46270808673987673 1106 84-85 -0.6244658184813794 1106 86-87 -0.4474048274531377 1106 88-89 -0.5115606242233532 1106 90-91 -0.6019696368768663 1106 92-93 -0.5931944079249796 1106 94-95 -1.185848464305824 1106 96-97 -0.7921206240677314 1106 98-99 -0.6551494193019778 1106 100-101 -0.24902204187116084 1107 1 -0.020284313137892696 1107 2 -0.04653333576636243 1107 3 -0.062018462621267645 1107 4 -0.026903082892481223 1107 5 -0.1402959270607269 1107 6 -0.06960856824711925 1107 7 -0.12242686896067312 1107 8 -0.1177274537330888 1107 9 -0.11140887009811706 1107 10-11 -0.06372712553123705 1107 12-13 -0.14587921370058865 1107 14-15 -0.2323997425009452 1107 16-17 -0.3085187416236792 1107 18-19 -0.16453972885918944 1107 20-21 -0.17216932321536405 1107 22-23 -0.09979761829884382 1107 24-25 -0.18335528557857828 1107 26-27 -0.18072571619639177 1107 28-29 -0.2152357397369471 1107 30-31 -0.13563833443244988 1107 32-33 -0.14011906566861398 1107 34-35 -0.11822764532658425 1107 36-37 -0.2673910944420399 1107 38-39 -0.18073980275294588 1107 40-41 -0.10915830436582752 1107 42-43 -0.16523991105481883 1107 44-45 -0.03949909825744413 1107 46-47 -0.09259965849901874 1107 48-49 -0.08979657951334019 1107 50-51 -0.1072918684805515 1107 52-53 -0.11015323274168765 1107 54-55 -0.19438922386309088 1107 56-57 -0.11684985917784019 1107 58-59 -0.06045715593442935 1107 60-61 -0.037773730245241666 1107 62-63 -0.1385432912037814 1107 64-65 -0.16190876990310699 1107 66-67 -0.06563064494200432 1107 68-69 -0.1524285355964068 1107 70-71 -0.25755588974590893 1107 72-73 -0.24003144163553003 1107 74-75 -0.13294712254801766 1107 76-77 -0.15444939052977347 1107 78-79 -0.0981644650229434 1107 80-81 -0.04229329282982164 1107 82-83 0.05339708867878201 1107 84-85 0.008250872729728798 1107 86-87 -0.12360376594706324 1107 88-89 -0.1783829586801282 1107 90-91 -0.027050271144862137 1107 92-93 0.06441377707095342 1107 94-95 0.012074054316656202 1107 96-97 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-0.4957647208946696 2316 56-57 -0.3741504132097191 2316 58-59 -0.5076619935248843 2316 60-61 -0.345230140162748 2316 62-63 -0.35567356028924024 2316 64-65 -0.5193419391196414 2316 66-67 -0.4541600923420219 2316 68-69 -0.2234382394151524 2316 70-71 -0.2708092574446397 2316 72-73 -0.29542669877021055 2316 74-75 -0.3080767087313667 2316 76-77 -0.3379482725529286 2316 78-79 -0.6347968764384255 2316 80-81 -0.47185942969662165 2316 82-83 -0.5140447820324283 2316 84-85 -0.6382395611333997 2316 86-87 -0.7455628138493431 2316 88-89 -0.4064612501030993 2316 90-91 -0.2541031079422744 2316 92-93 -0.33749812855637984 2316 94-95 -0.25950378000248264 2316 96-97 -0.18980700654272908 2316 98-99 0.15689560844473505 2316 100-101 -0.397451287674901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 28.0 5 67.0 6 154.0 7 386.0 8 992.0 9 1878.0 10 3252.0 11 5135.0 12 7708.0 13 10610.0 14 13847.0 15 18261.0 16 23580.0 17 31534.0 18 28007.0 19 18069.0 20 15627.0 21 16119.0 22 16887.0 23 18659.0 24 22222.0 25 26170.0 26 32501.0 27 40012.0 28 49953.0 29 63126.0 30 78413.0 31 97998.0 32 124183.0 33 161041.0 34 218315.0 35 306945.0 36 463464.0 37 698627.0 38 573457.0 39 55822.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.84091718223512 20.0696241075632 15.728730063435433 22.360728646766248 2 15.527791730610726 21.205530933816085 39.62098863761456 23.64568869795863 3 18.655939790365235 25.24645636586826 30.662098154556688 25.43550568920982 4 13.086123051960966 17.818307288559076 37.39386981898936 31.701699840490598 5 13.373316774140026 37.76492216367017 34.48884798777737 14.372913074412438 6 31.093417993881722 37.319033298397294 17.483685977695817 14.10386273002517 7 27.647916217632122 33.397038422555916 21.20247826981171 17.752567090000245 8 26.16062131271336 35.14562903225309 19.969726206873798 18.724023448159752 9 26.167012224227182 16.853763165003016 20.60203486328378 36.37718974748602 10-11 24.32427432163793 27.256512272788523 27.63017068091983 20.789042724653715 12-13 25.478398264300147 24.55063388136198 28.24288141964263 21.72808643469525 14-15 22.414123782133526 25.889782178547545 26.65378072436942 25.04231331494951 16-17 21.575458308164272 29.11263990991249 27.323963813289204 21.98793796863403 18-19 21.61549754260548 27.36688611929009 30.051935373561278 20.965680964543154 20-21 22.24789235134991 26.46761447258754 30.28231900092166 21.00217417514089 22-23 22.030924014028784 26.32437221056994 30.193250419513014 21.451453355888262 24-25 21.850080949694632 26.596734846542475 30.211542265515305 21.341641938247584 26-27 21.630638446406632 26.879638881646876 30.02525565160922 21.464467020337267 28-29 21.639966542937206 26.993744145977637 30.04727930094782 21.319010010137333 30-31 21.742306438746756 26.658328885386034 30.070112600132777 21.529252075734433 32-33 21.565159421523255 26.713523517384303 29.913285841281823 21.808031219810616 34-35 22.06656719298911 26.749569620627867 29.736971368786786 21.446891817596235 36-37 22.082277620365154 26.632211648904168 29.64902997746887 21.636480753261804 38-39 21.814090295334452 26.849027880689558 29.659945563908753 21.67693626006724 40-41 22.09336279924972 26.779109540993424 29.555923725335475 21.571603934421375 42-43 21.923712754699316 26.80286198601204 29.453811918218666 21.81961334106999 44-45 21.990296845364686 27.014180086825164 29.327189769937057 21.668333297873094 46-47 22.21813658605475 27.278158435728628 28.69351530910998 21.810189669106638 48-49 22.241740770856246 27.368258276342562 28.455191671839238 21.934809280961954 50-51 22.335324965333765 27.484413683458474 28.378273789426007 21.801987561781758 52-53 22.53806502421009 27.47645825605314 28.152792925466585 21.83268379427018 54-55 22.38105325541948 27.47581072126433 28.247379257117256 21.895756766198932 56-57 22.316006844134368 27.77777777777778 28.086112259714486 21.820103118373368 58-59 22.41082443820961 27.38419996614318 28.101159734806753 22.103815860840456 60-61 22.197893415157065 27.423622500785523 28.45295613506836 21.92552794898906 62-63 22.112310900569803 27.551078931715605 28.531107417079316 21.805502750635277 64-65 22.126371655983885 27.408343763268682 28.36637148330794 22.098913097439493 66-67 22.123534836909112 27.573418881929427 28.225933521611786 22.077112759549674 68-69 22.198525532450898 27.42820149679208 28.34048550925065 22.03278746150637 70-71 22.12711169574252 27.37963638763159 28.228785758181523 22.264466158444364 72-73 22.238314680710992 27.442974470138587 28.009691449292568 22.309019399857853 74-75 22.229961804697957 27.76357826490895 27.847464855049335 22.158995075343757 76-77 22.318581565794624 27.501989627726083 28.043482886118053 22.135945920361237 78-79 22.29986472689912 27.607522380806575 27.94104904952685 22.15156384276745 80-81 22.231118116820824 27.72878097875808 27.88414307558674 22.155957828834357 82-83 22.264736926009103 27.725133754807572 27.829248355180493 22.180880964002835 84-85 22.201249076482654 27.67544054398409 27.834889899464578 22.288420480068677 86-87 22.279775730951144 27.53777363355513 27.81670695258103 22.365743682912697 88-89 22.228651317625374 27.54460358382754 27.711266704470365 22.51547839407672 90-91 22.197046185272743 27.513104731148708 27.819159335891268 22.470689747687285 92-93 22.2407148362345 27.27599581441652 27.91129547878971 22.57199387055927 94-95 21.97040827685134 27.351329866863765 27.927466236456887 22.75079561982801 96-97 22.080042083594275 27.26534649740724 27.86917268796933 22.78543873102916 98-99 21.88544874314055 27.368828886637587 27.79314990286191 22.952572467359953 100-101 21.78888638918747 27.315746777402584 28.035414070056845 22.859952763353096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 500.0 1 384.0 2 358.5 3 476.0 4 448.0 5 421.0 6 698.0 7 1545.0 8 2674.5 9 3366.0 10 3269.0 11 3012.5 12 3319.0 13 3762.5 14 4033.0 15 4301.0 16 4654.5 17 4968.0 18 5247.0 19 5781.5 20 6463.5 21 7366.0 22 8805.5 23 10614.5 24 12977.5 25 16411.5 26 20866.0 27 26301.0 28 31122.5 29 36359.0 30 43067.0 31 49770.0 32 57043.0 33 62793.0 34 67673.5 35 73571.5 36 80917.0 37 88409.0 38 93576.5 39 96853.0 40 99834.5 41 105071.0 42 109428.5 43 113701.0 44 119432.5 45 122817.0 46 122883.0 47 123406.5 48 123728.0 49 120792.0 50 117097.5 51 111766.0 52 105987.0 53 103719.5 54 102701.0 55 95074.5 56 83375.0 57 74090.5 58 68184.0 59 60814.0 60 49473.5 61 39647.0 62 32538.0 63 24888.5 64 17987.0 65 13430.5 66 9546.5 67 7247.0 68 5487.0 69 3805.5 70 2781.0 71 2063.5 72 1574.0 73 1334.0 74 1109.5 75 819.5 76 574.0 77 359.0 78 216.5 79 139.5 80 78.0 81 49.5 82 38.0 83 22.0 84 10.0 85 5.0 86 1.5 87 2.5 88 3.5 89 2.5 90 1.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05127858827548844 2 0.0 3 0.0 4 0.0 5 0.017267594368174096 6 0.0 7 0.0 8 0.0 9 3.083498994316803E-5 10-11 0.0 12-13 0.003453518873634819 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.092047686928646E-4 24-25 1.6959244468742416E-4 26-27 0.0010021371731529609 28-29 0.0032839264289473953 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015278737516839759 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.2333995977267212E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.4667991954534425E-4 84-85 0.001017554668124545 86-87 0.0 88-89 0.0 90-91 0.010699741510279305 92-93 0.0010021371731529609 94-95 0.0 96-97 0.0 98-99 1.6959244468742416E-4 100-101 0.01416867787888571 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3243069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.72215155595135 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82486325671265 35.71286736335748 2 14.913833195610943 14.830957488646913 3 5.464299642745229 8.150902049511274 4 2.5728444399810075 5.117094446984898 5 1.3963376071076259 3.4714455061939917 6 0.8510313099579309 2.538906466355283 7 0.5447848125285715 1.8961511109748326 8 0.3969992401844352 1.579172511043842 9 0.30434754832483835 1.3619533431140858 >10 1.5579898144481141 14.177838936974457 >50 0.11652073359519093 3.9878916942950324 >100 0.05205468715580094 4.476079008176857 >500 0.0029152189014964303 1.0087969725093222 >1k 9.924149441454548E-4 0.680128389396216 >5k 6.202593400909092E-5 0.23395701092639073 >10k+ 1.2405186801818185E-4 0.7758577015390472 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14014 0.43212154906355676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11143 0.34359429293672133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7586 0.23391423370887268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.083498994316803E-5 2 0.0 0.0 0.0 0.0 3.083498994316803E-5 3 0.0 0.0 0.0 0.0 3.083498994316803E-5 4 0.0 0.0 0.0 0.0 3.083498994316803E-5 5 0.0 0.0 0.0 0.0 3.083498994316803E-5 6 0.0 0.0 0.0 0.0 6.166997988633606E-5 7 6.166997988633606E-5 0.0 0.0 0.0 6.166997988633606E-5 8 6.166997988633606E-5 0.0 0.0 0.0 6.166997988633606E-5 9 6.166997988633606E-5 0.0 0.0 0.0 6.166997988633606E-5 10-11 7.708747485792008E-5 0.0 0.0 0.0 6.166997988633606E-5 12-13 1.3875745474425613E-4 0.0 0.0 3.083498994316803E-5 1.6959244468742418E-4 14-15 1.5417494971584016E-4 0.0 0.0 3.083498994316803E-5 1.8500993965900819E-4 16-17 2.3126242457376025E-4 0.0 0.0 3.083498994316803E-5 1.8500993965900819E-4 18-19 3.237673944032643E-4 0.0 0.0 4.6252484914752047E-5 1.8500993965900819E-4 20-21 3.391848893748483E-4 0.0 0.0 6.166997988633606E-5 2.1584492960217622E-4 22-23 3.391848893748483E-4 0.0 0.0 1.2333995977267212E-4 2.1584492960217622E-4 24-25 3.391848893748483E-4 0.0 0.0 1.8500993965900819E-4 2.1584492960217622E-4 26-27 3.391848893748483E-4 0.0 0.0 4.933598390906885E-4 2.3126242457376025E-4 28-29 3.391848893748483E-4 0.0 0.0 0.0010946421429824651 2.4667991954534425E-4 30-31 3.391848893748483E-4 0.0 0.0 0.002220119275908098 2.4667991954534425E-4 32-33 3.5460238434643237E-4 0.0 0.0 0.003962296207697092 2.4667991954534425E-4 34-35 4.008548692611844E-4 0.0 0.0 0.0059819880489745975 2.4667991954534425E-4 36-37 4.6252484914752044E-4 0.0 0.0 0.008865059608660809 2.4667991954534425E-4 38-39 4.6252484914752044E-4 0.0 0.0 0.013860327979454029 2.7751490948851225E-4 40-41 4.6252484914752044E-4 0.0 0.0 0.018716838895502994 2.7751490948851225E-4 42-43 4.6252484914752044E-4 0.0 0.0 0.024452147024932248 2.7751490948851225E-4 44-45 5.241948290338565E-4 0.0 0.0 0.02906197802143587 2.9293240446009625E-4 46-47 6.012823038917766E-4 0.0 0.0 0.03547565592961482 3.083498994316803E-4 48-49 6.166997988633606E-4 0.0 0.0 0.041889333837793766 3.391848893748483E-4 50-51 6.166997988633606E-4 0.0 0.0 0.0481334193012853 3.7001987931801637E-4 52-53 6.166997988633606E-4 0.0 0.0 0.06299588445389229 4.008548692611844E-4 54-55 6.166997988633606E-4 0.0 0.0 0.08738636149893819 4.3168985920435243E-4 56-57 6.166997988633606E-4 0.0 0.0 0.12246116255929182 4.3168985920435243E-4 58-59 6.166997988633606E-4 0.0 0.0 0.16959244468742418 4.3168985920435243E-4 60-61 6.166997988633606E-4 0.0 0.0 0.23044529734026625 4.4710735417593644E-4 62-63 6.166997988633606E-4 0.0 0.0 0.26989866697254977 5.241948290338565E-4 64-65 6.166997988633606E-4 0.0 0.0 0.32187104252175947 5.550298189770245E-4 66-67 6.475347888065286E-4 0.0 0.0 0.4124025729949008 5.704473139486086E-4 68-69 6.475347888065286E-4 0.0 0.0 0.5308243518716376 5.858648089201926E-4 70-71 6.783697787496967E-4 0.0 0.0 0.6522062897829186 5.858648089201926E-4 72-73 7.092047686928647E-4 0.0 0.0 0.7924900765293615 6.475347888065286E-4 74-75 7.092047686928647E-4 0.0 0.0 0.9319104835573957 6.783697787496966E-4 76-77 7.092047686928647E-4 0.0 0.0 1.072625960163043 6.783697787496966E-4 78-79 7.092047686928647E-4 0.0 0.0 1.2104429477140326 6.783697787496966E-4 80-81 7.092047686928647E-4 0.0 0.0 1.3556603328513823 6.937872737212806E-4 82-83 7.400397586360327E-4 0.0 0.0 1.5515396064653575 7.708747485792007E-4 84-85 7.400397586360327E-4 0.0 0.0 1.8239050726333605 7.708747485792007E-4 86-87 7.400397586360327E-4 0.0 0.0 2.1348605287152385 8.017097385223688E-4 88-89 7.400397586360327E-4 0.0 0.0 2.510554046182798 8.325447284655369E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7045 0.0 52.48552 1 GTATCAA 12660 0.0 41.257687 1 ATCAACG 16535 0.0 31.40238 3 TATCAAC 16745 0.0 31.036932 2 TCAACGC 16745 0.0 30.980192 4 CAACGCA 17070 0.0 30.446012 5 AACGCAG 17665 0.0 29.415075 6 ACGCAGA 20225 0.0 25.62454 7 CGCAGAG 20310 0.0 25.423742 8 GTACATG 11990 0.0 23.862623 1 GCAGAGT 22065 0.0 23.207836 9 TACATGG 12240 0.0 23.054285 2 ACATGGG 12240 0.0 22.16161 3 TGGTATC 3335 0.0 22.079157 2 GAGTACT 13695 0.0 21.817675 12-13 AGAGTAC 20475 0.0 20.880383 10-11 AGTACTT 14370 0.0 20.74325 12-13 GTACTTT 14550 0.0 20.320885 14-15 CAGAGTA 21700 0.0 19.778267 10-11 CATGGGG 6395 0.0 19.760035 4 >>END_MODULE