FastQCFastQC Report
Thu 26 May 2016
SRR937520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492422
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127110.36395945278090674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95590.2737069002543221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65880.18863699747625No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54530.15613806120795254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66500.052.8123441
GTATCAA122250.043.306091
ATCAACG157550.033.0214423
TCAACGC159950.032.5287744
TATCAAC160850.032.4039842
CAACGCA162250.032.1253055
AACGCAG167350.031.1427026
ACGCAGA192550.026.8937997
CGCAGAG194500.026.4280038
GTACATG130250.024.4825941
GCAGAGT209850.023.9968139
TACATGG131700.023.8827882
ACATGGG132900.023.0944633
GAGTACT126600.021.72568312-13
GGACCGA7500.020.9039366
AGTACTT130400.020.89221412-13
TGGTATC33250.020.862872
AGAGTAC195550.020.8064610-11
CAGAGTA204200.020.3438310-11
GTACTTT136100.020.12049314-15
Produced by FastQC (version 0.11.5)