FastQCFastQC Report
Thu 26 May 2016
SRR937520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492422
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148160.42423281035338806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117000.3350110610917008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.23167303378572235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.049.734611
GTATCAA134850.042.9942861
ATCAACG171950.033.6175773
TATCAAC175800.032.9894262
TCAACGC176000.032.8174
CAACGCA179650.032.150255
AACGCAG185550.031.1535556
ACGCAGA211350.027.2850957
CGCAGAG211350.027.2626198
GCAGAGT231000.024.655649
GTACATG132500.023.7075921
TACATGG133350.022.8675692
GAGTACT140750.022.5443212-13
ACATGGG135400.021.6443463
AGTACTT145800.021.61685212-13
AGAGTAC212650.021.39977310-11
GTACTTT147550.021.26236514-15
CAGAGTA225750.020.42099210-11
TACTTTT171250.018.25043914-15
ACTTTTT181100.017.69081116-17
Produced by FastQC (version 0.11.5)