##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3492422 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.097962674613775 31.0 31.0 34.0 30.0 34.0 2 32.30560940230018 34.0 31.0 34.0 31.0 34.0 3 32.359360065879784 34.0 31.0 34.0 30.0 34.0 4 36.02794507651137 37.0 35.0 37.0 35.0 37.0 5 35.93535374591043 37.0 35.0 37.0 35.0 37.0 6 35.96682388325351 37.0 35.0 37.0 35.0 37.0 7 35.9033667752637 37.0 35.0 37.0 35.0 37.0 8 35.87557918258447 37.0 35.0 37.0 35.0 37.0 9 37.56789872472456 39.0 37.0 39.0 35.0 39.0 10-11 37.482598179715964 39.0 37.0 39.0 35.0 39.0 12-13 37.383100037738856 39.0 37.0 39.0 34.0 39.0 14-15 38.56319854817087 40.0 38.0 41.0 34.0 41.0 16-17 38.52879176685979 40.0 38.0 41.0 34.0 41.0 18-19 38.516255337986074 40.0 38.0 41.0 34.0 41.0 20-21 38.41742464112298 40.0 38.0 41.0 34.0 41.0 22-23 38.267372900525764 40.0 38.0 41.0 34.0 41.0 24-25 38.206787438631416 40.0 38.0 41.0 33.5 41.0 26-27 38.14594012407436 40.0 38.0 41.0 33.5 41.0 28-29 38.01097533459588 40.0 37.0 41.0 33.0 41.0 30-31 38.07108734282397 40.0 37.0 41.0 33.0 41.0 32-33 38.00972978065079 40.0 37.0 41.0 33.0 41.0 34-35 37.88264175978733 40.0 37.0 41.0 33.0 41.0 36-37 37.70416719399889 40.0 37.0 41.0 33.0 41.0 38-39 37.569347719147345 40.0 37.0 41.0 33.0 41.0 40-41 37.38595536278262 40.0 37.0 41.0 32.0 41.0 42-43 37.173031065547065 40.0 36.0 41.0 31.5 41.0 44-45 36.89502027532755 40.0 36.0 41.0 31.0 41.0 46-47 36.562017133095594 39.0 36.0 41.0 30.5 41.0 48-49 36.24868415099893 39.0 35.5 41.0 30.0 41.0 50-51 36.00691855108003 39.0 35.0 40.5 29.0 41.0 52-53 35.75439136507558 39.0 35.0 40.0 28.5 41.0 54-55 35.51811493570937 39.0 35.0 40.0 28.0 41.0 56-57 35.243563778947674 38.0 34.5 40.0 27.0 41.0 58-59 34.96790193166805 38.0 34.0 40.0 26.5 41.0 60-61 34.64273675976156 38.0 34.0 40.0 26.0 41.0 62-63 34.27151859082322 37.0 34.0 40.0 26.0 41.0 64-65 33.87155761818016 37.0 33.0 39.5 25.0 41.0 66-67 33.45386511137543 36.0 33.0 39.0 24.0 40.5 68-69 33.019998012840375 36.0 32.0 39.0 23.0 40.0 70-71 32.60987131566574 35.0 32.0 38.0 22.0 40.0 72-73 32.13782770237961 35.0 31.0 37.5 21.5 39.5 74-75 31.661047834425506 35.0 31.0 37.0 20.0 39.0 76-77 30.40309103539034 33.5 29.5 35.5 19.5 38.0 78-79 31.0096957068762 34.0 31.0 36.0 19.5 38.5 80-81 30.82909482301967 34.0 31.0 36.0 18.0 37.5 82-83 30.475095793120076 34.0 30.5 35.0 17.5 37.0 84-85 30.10546520437679 34.0 30.0 35.0 13.0 36.5 86-87 29.684584508974 34.0 30.0 35.0 7.5 36.0 88-89 29.20519971526923 34.0 29.0 35.0 4.5 36.0 90-91 28.683983922905078 34.0 29.0 35.0 2.0 35.0 92-93 28.30827274023586 33.5 29.0 35.0 2.0 35.0 94-95 27.850189352833077 33.0 27.5 35.0 2.0 35.0 96-97 27.353597159793402 33.0 27.0 35.0 2.0 35.0 98-99 26.57355869365157 33.0 25.5 34.5 2.0 35.0 100-101 24.083507806330392 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0019216326186537458 1101 2 0.014962135981747338 1101 3 -0.00693283720622162 1101 4 0.039308396491883 1101 5 0.013823167229489286 1101 6 -0.07815658807032122 1101 7 -0.02913210030053648 1101 8 -0.013543426158825866 1101 9 -0.048498048462036536 1101 10-11 -0.02046956058592997 1101 12-13 -0.056282468347028214 1101 14-15 -0.08265198509694471 1101 16-17 -0.034166643730529245 1101 18-19 0.003349867088878966 1101 20-21 -0.007754005246454199 1101 22-23 0.021066859255221004 1101 24-25 -0.05800932678470616 1101 26-27 -0.07084413686440882 1101 28-29 -0.1468909877111244 1101 30-31 -0.10107045097104361 1101 32-33 -0.00972608510903683 1101 34-35 -0.08129121011638318 1101 36-37 -0.07279530388274935 1101 38-39 -0.12469732162876568 1101 40-41 -0.038837730963813044 1101 42-43 -0.005728206540283054 1101 44-45 0.08000117792258976 1101 46-47 -0.0045995376699536905 1101 48-49 -0.08779645995997498 1101 50-51 -0.050221161478688714 1101 52-53 -0.009279095444348684 1101 54-55 -0.04089463807044069 1101 56-57 0.0670488427219027 1101 58-59 0.05841952895166003 1101 60-61 0.0854623518379114 1101 62-63 0.04461530466467423 1101 64-65 -0.019619093502420526 1101 66-67 0.05663996794679349 1101 68-69 -0.0029479360620570105 1101 70-71 0.07924937442786728 1101 72-73 0.07518283499867096 1101 74-75 0.010812815374233509 1101 76-77 0.035895846694721456 1101 78-79 -0.16582847624903607 1101 80-81 -0.045125563858825046 1101 82-83 0.04347640769687189 1101 84-85 0.05326932601129286 1101 86-87 -0.054583868863208096 1101 88-89 -0.09011984337489665 1101 90-91 -0.1120922101138575 1101 92-93 -0.0021434074926744984 1101 94-95 0.08712517228848782 1101 96-97 0.0650414853760033 1101 98-99 0.18930258883121454 1101 100-101 0.6820740582216871 1102 1 -0.06610171054892078 1102 2 -0.001495513821701877 1102 3 -0.0979335343831309 1102 4 -0.04077170655702389 1102 5 -0.05363665080042779 1102 6 -0.07767283908167144 1102 7 -0.14467128711189048 1102 8 -0.040194443540471525 1102 9 -0.16622740174715744 1102 10-11 -0.10894923086966202 1102 12-13 -0.14027103690893483 1102 14-15 -0.07823779914999562 1102 16-17 -0.09720954117008773 1102 18-19 -0.08990590505182894 1102 20-21 0.027833575323370496 1102 22-23 -0.02676698884068429 1102 24-25 -0.12927479402210196 1102 26-27 -0.09663785925472723 1102 28-29 -0.15141147899139185 1102 30-31 -0.09657189970339886 1102 32-33 -0.009319126164520242 1102 34-35 -0.14365296131915528 1102 36-37 -0.14820743350359322 1102 38-39 -0.206200413661783 1102 40-41 -0.14547471050930483 1102 42-43 -0.14987644684439516 1102 44-45 -0.27295578884866245 1102 46-47 -0.16527435965095805 1102 48-49 -0.06275671333253996 1102 50-51 -0.24582762680435621 1102 52-53 -0.1749081482958914 1102 54-55 -0.18253706489987565 1102 56-57 -0.1753100921124897 1102 58-59 -0.22582590749428277 1102 60-61 -0.2764579901999653 1102 62-63 -0.18103253864465785 1102 64-65 -0.17119723420894672 1102 66-67 -0.26253406287358416 1102 68-69 -0.16637896426911425 1102 70-71 -0.15189881118338633 1102 72-73 -0.12251981307912274 1102 74-75 -0.11923051143577013 1102 76-77 -0.016909143055080733 1102 78-79 -0.09739000163941469 1102 80-81 -0.13851787957371897 1102 82-83 -0.07966772812752154 1102 84-85 -0.14267016858888582 1102 86-87 -0.0616450953287746 1102 88-89 0.015036373915464196 1102 90-91 -0.09581611548639657 1102 92-93 0.10081893426734112 1102 94-95 0.0801739287801233 1102 96-97 0.1374756197607816 1102 98-99 0.35923515315348453 1102 100-101 1.1758525244450695 1103 1 0.00573307602177664 1103 2 -0.011039830446577525 1103 3 -0.042761463702348124 1103 4 -0.0804024169422064 1103 5 -0.03892613080096652 1103 6 -0.05771984692279375 1103 7 -0.09184812152486188 1103 8 -0.14741819409854884 1103 9 -0.1633093617570367 1103 10-11 -0.09973316121637055 1103 12-13 -0.12237742585396205 1103 14-15 -0.09831293908246153 1103 16-17 -0.20307996998405287 1103 18-19 -0.16788404620744046 1103 20-21 -0.2702731001097618 1103 22-23 -0.15126853113057592 1103 24-25 -0.15060028354564992 1103 26-27 -0.1683613714651102 1103 28-29 -0.2583032341932494 1103 30-31 -0.29367432403466154 1103 32-33 -0.22715587949094385 1103 34-35 -0.13779600190716934 1103 36-37 -0.20766235889958296 1103 38-39 -0.15407652316813625 1103 40-41 -0.2568748120936277 1103 42-43 -0.23147335906764255 1103 44-45 -0.350602678129313 1103 46-47 -0.18399069466509843 1103 48-49 -0.38372769860504263 1103 50-51 -0.318806445147473 1103 52-53 -0.17714689425120156 1103 54-55 -0.3110233569023819 1103 56-57 -0.45215747883165847 1103 58-59 -0.4892172129519423 1103 60-61 -0.37710270616100416 1103 62-63 -0.3299982694873478 1103 64-65 -0.4793750446888865 1103 66-67 -0.265957639134875 1103 68-69 -0.34998810229802046 1103 70-71 -0.23272113985835574 1103 72-73 -0.10016066777029664 1103 74-75 -0.019433983666687027 1103 76-77 0.03877032259999069 1103 78-79 -0.11302042479300667 1103 80-81 -0.20623139468739282 1103 82-83 -0.14257962448425232 1103 84-85 -0.0014389970966348642 1103 86-87 -0.08365622945613893 1103 88-89 -0.09596122550749442 1103 90-91 -0.0021386552480819887 1103 92-93 -0.015718158074143673 1103 94-95 0.02768567051753834 1103 96-97 0.15779468349980164 1103 98-99 0.33555930255207755 1103 100-101 0.8215876743674428 1104 1 -0.015805338175667316 1104 2 -0.013937133340498065 1104 3 -0.06061301034042543 1104 4 -0.046956575973780446 1104 5 -0.11910875760284512 1104 6 -0.08780753409445197 1104 7 -0.06301326338524404 1104 8 -0.026522062941268132 1104 9 -0.014053342125919244 1104 10-11 -0.07100953358727935 1104 12-13 -0.023003418013836097 1104 14-15 -0.07479023532729201 1104 16-17 -0.16336054117851262 1104 18-19 -0.13055593762087625 1104 20-21 -0.15857682124850925 1104 22-23 -0.3124821775786586 1104 24-25 -0.10434736702554659 1104 26-27 -0.22704525537992026 1104 28-29 -0.28240921179992284 1104 30-31 -0.20064832921999454 1104 32-33 -0.15395673071960658 1104 34-35 -0.15286548854532356 1104 36-37 -0.1990279208233332 1104 38-39 -0.18672779153384766 1104 40-41 -0.2976337197357992 1104 42-43 -0.20620068291646731 1104 44-45 -0.08841254424844891 1104 46-47 -0.08578190712290734 1104 48-49 -0.0725197237967734 1104 50-51 -0.13399518706312108 1104 52-53 -0.11878004896809813 1104 54-55 -0.059683341492416275 1104 56-57 -0.16493170110805266 1104 58-59 -0.18663178991096885 1104 60-61 -0.08187035295541278 1104 62-63 -0.09311638017965151 1104 64-65 -0.11390652198814877 1104 66-67 0.014309941590965991 1104 68-69 -0.06289672433693028 1104 70-71 -0.11495448680764753 1104 72-73 -0.08092828801660801 1104 74-75 -0.012402809558807348 1104 76-77 0.04306655958765049 1104 78-79 -0.0025477117158310136 1104 80-81 -0.09183215710059045 1104 82-83 -0.13109986348956681 1104 84-85 -0.004807770133901101 1104 86-87 -0.11128960500497342 1104 88-89 -0.08552043048840119 1104 90-91 -0.16255504411501676 1104 92-93 0.022358938818580754 1104 94-95 0.1682186176162226 1104 96-97 -0.00319867404459373 1104 98-99 0.11023618500854582 1104 100-101 0.7312951685947375 1105 1 -0.04366379378988938 1105 2 -0.03194874394758074 1105 3 -0.01412915725252617 1105 4 -0.05991844767365251 1105 5 -0.10333583540558067 1105 6 -0.15280359582320813 1105 7 -0.13871033739556538 1105 8 -0.11553632823488869 1105 9 -0.0967733747989854 1105 10-11 -0.07570862049180249 1105 12-13 -0.08058706981230301 1105 14-15 -0.09874980240917353 1105 16-17 -0.1417656871862718 1105 18-19 -0.1353124941131867 1105 20-21 -0.08510705841995048 1105 22-23 -0.11731691604827432 1105 24-25 -0.1841171969505737 1105 26-27 -0.07918709148948722 1105 28-29 -0.1313861364476665 1105 30-31 -0.1958243845308303 1105 32-33 -0.24680512186287729 1105 34-35 -0.26898118620277955 1105 36-37 -0.18179900516067704 1105 38-39 -0.2721620224040535 1105 40-41 -0.35267091451977706 1105 42-43 -0.2650197849906206 1105 44-45 -0.3311375362260449 1105 46-47 -0.3194535428182377 1105 48-49 -0.18038080492516428 1105 50-51 -0.18148782103727257 1105 52-53 -0.18983550566170493 1105 54-55 -0.011846765162886186 1105 56-57 -0.143113678411936 1105 58-59 -0.060815692591909 1105 60-61 -0.18739326785958355 1105 62-63 -0.10948412719677947 1105 64-65 -0.15624818889791214 1105 66-67 -0.18353626162382852 1105 68-69 -0.10331593143165207 1105 70-71 -0.15618206054083572 1105 72-73 -0.19356634630302239 1105 74-75 -0.11729968519695078 1105 76-77 0.04982346614851707 1105 78-79 -0.06698074651848174 1105 80-81 -0.15435469276382463 1105 82-83 -0.16289192276784092 1105 84-85 -0.06235874886723636 1105 86-87 0.014441308965679411 1105 88-89 0.0025054745846802007 1105 90-91 0.2543515167531538 1105 92-93 0.39921007216552695 1105 94-95 0.4787101722123275 1105 96-97 0.5750212273527602 1105 98-99 0.6281891591326456 1105 100-101 1.109268855108347 1106 1 -0.19569046125378975 1106 2 -0.0430336792221766 1106 3 -0.05727304795180288 1106 4 -0.06334396114191776 1106 5 -0.21496189298577661 1106 6 -0.11493699293522752 1106 7 -0.15638440836929135 1106 8 -0.13569178878677945 1106 9 -0.14347252873483285 1106 10-11 -0.2590973615602934 1106 12-13 -0.21795111657005606 1106 14-15 -0.26693390357812063 1106 16-17 -0.14464918146114059 1106 18-19 -0.15023371746814718 1106 20-21 -0.15473006658163513 1106 22-23 -0.21128594352293106 1106 24-25 -0.2240188382552475 1106 26-27 -0.21713381635420603 1106 28-29 -0.25095234171539005 1106 30-31 -0.14918352830193982 1106 32-33 -0.1325909450563998 1106 34-35 -0.11889508497404933 1106 36-37 -0.0970428091607971 1106 38-39 -0.18331332737580652 1106 40-41 -0.01288885825501751 1106 42-43 -0.11947701839071811 1106 44-45 0.04162308442031559 1106 46-47 -0.24032621464698423 1106 48-49 -0.17447569565433696 1106 50-51 -0.0105529649877667 1106 52-53 -0.0052811094166784756 1106 54-55 -0.10003295907002752 1106 56-57 -0.19451581300490517 1106 58-59 -0.1338238542708794 1106 60-61 0.03651686398291787 1106 62-63 0.10120886810950225 1106 64-65 0.049391142621708184 1106 66-67 -0.016382684536324632 1106 68-69 -0.08530403272018106 1106 70-71 0.031936866781357764 1106 72-73 7.689103563706112E-4 1106 74-75 0.0724880513572046 1106 76-77 0.12440506799012496 1106 78-79 -0.03915181291807457 1106 80-81 -0.14596550325143198 1106 82-83 -0.2038826848235118 1106 84-85 -0.13429094414960474 1106 86-87 -0.07566093624344816 1106 88-89 0.12427174562417065 1106 90-91 0.15287643528584738 1106 92-93 0.29663660210663423 1106 94-95 0.36345440970822196 1106 96-97 0.24963930263154666 1106 98-99 0.2677984150147381 1106 100-101 1.0145572045358335 1107 1 -0.11360537389507641 1107 2 -0.1555180392667026 1107 3 -0.0822630713854764 1107 4 -0.0914421725611092 1107 5 -0.11938338716036156 1107 6 -0.19117716264187123 1107 7 -0.17336047361153106 1107 8 -0.11657098682086797 1107 9 -0.11195468043264611 1107 10-11 -0.06124539880213575 1107 12-13 -0.1207949630539602 1107 14-15 -0.1443145523399778 1107 16-17 -0.08741624589026031 1107 18-19 -0.14229957180727837 1107 20-21 -0.11937491582295934 1107 22-23 -0.07932402550180484 1107 24-25 -0.14875757223648378 1107 26-27 -0.23737709454889 1107 28-29 -0.17849471586813337 1107 30-31 -0.15820459460935155 1107 32-33 -0.14971373333415983 1107 34-35 -0.17652813336264472 1107 36-37 -0.1493906777761751 1107 38-39 -0.04988087848132494 1107 40-41 -0.19088670391678875 1107 42-43 -0.17029374661705532 1107 44-45 -0.030783083348019602 1107 46-47 -0.16524002611538435 1107 48-49 -0.059467939436615325 1107 50-51 -0.03883765568420472 1107 52-53 -0.2149940843537408 1107 54-55 0.008879040113839665 1107 56-57 -0.06568473652588835 1107 58-59 -0.13296571954372638 1107 60-61 -0.03966479653778521 1107 62-63 -0.06339607486653875 1107 64-65 -0.16676129331072786 1107 66-67 -0.08596585909472765 1107 68-69 0.05583122551536235 1107 70-71 0.13213774831975655 1107 72-73 0.17986687873739982 1107 74-75 0.22119319806523663 1107 76-77 0.26302068064131134 1107 78-79 0.18128559575508518 1107 80-81 0.23791581611570933 1107 82-83 0.16080237073020953 1107 84-85 0.211919160836878 1107 86-87 0.1746404610759349 1107 88-89 0.2374727681847162 1107 90-91 0.39984895997222125 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0.6592716004320422 2315 32-33 0.605897247717742 2315 34-35 0.7299814674810037 2315 36-37 0.6537853784094025 2315 38-39 0.7111168732296278 2315 40-41 0.6553580812587327 2315 42-43 0.6919686290234921 2315 44-45 0.7143741267943255 2315 46-47 0.5622002334120069 2315 48-49 0.5339657886101037 2315 50-51 0.5639047466760303 2315 52-53 0.60543519350324 2315 54-55 0.60764665609247 2315 56-57 0.6892901055217564 2315 58-59 0.6000677388319957 2315 60-61 0.504669561462812 2315 62-63 0.5561004104422622 2315 64-65 0.5895837834929267 2315 66-67 0.8215808307867363 2315 68-69 0.6719208725618202 2315 70-71 0.9604390530696136 2315 72-73 0.9785799883963904 2315 74-75 1.042914932866097 2315 76-77 0.8964673819652376 2315 78-79 0.7150016588061412 2315 80-81 0.8686930994279578 2315 82-83 0.9269711099255886 2315 84-85 1.0435175953844507 2315 86-87 0.9111231257468688 2315 88-89 1.0827960486456263 2315 90-91 1.15156656620929 2315 92-93 1.20592563004924 2315 94-95 1.3410601860785398 2315 96-97 1.584464113988819 2315 98-99 1.5558107965775676 2315 100-101 1.4263722045675635 2316 1 -0.5712427346601174 2316 2 -0.3397750277334488 2316 3 0.01309564288740006 2316 4 0.0813030394704839 2316 5 0.191261923692295 2316 6 0.0878495376329056 2316 7 0.13449802123108157 2316 8 0.1738974787265164 2316 9 0.1849899209116117 2316 10-11 0.3382595665175572 2316 12-13 0.3514673991309323 2316 14-15 0.32228021004027596 2316 16-17 0.36614126084496235 2316 18-19 0.3152724245537897 2316 20-21 0.38419999044158715 2316 22-23 0.4023070280629284 2316 24-25 0.49599618570043447 2316 26-27 0.44551777408725 2316 28-29 0.5923682491562303 2316 30-31 0.5732493141923314 2316 32-33 0.47378946828249724 2316 34-35 0.4508855737490691 2316 36-37 0.5361983814823859 2316 38-39 0.4898537606255857 2316 40-41 0.44124315336576814 2316 42-43 0.5334983181649591 2316 44-45 0.3593668210836398 2316 46-47 0.37886948456028335 2316 48-49 0.041118068450487044 2316 50-51 0.20167608994397312 2316 52-53 0.6182758809607876 2316 54-55 0.5054540692593434 2316 56-57 0.4851517316484575 2316 58-59 0.5639530664751007 2316 60-61 0.22519909990352716 2316 62-63 0.3875402469865534 2316 64-65 0.3873239819374348 2316 66-67 0.42174796666422054 2316 68-69 0.41828603578820633 2316 70-71 0.6296407867835612 2316 72-73 0.5665294424883527 2316 74-75 0.6241855040656503 2316 76-77 0.6787711269882877 2316 78-79 0.5889980791171077 2316 80-81 0.4030807192514416 2316 82-83 0.43660804122133356 2316 84-85 0.3394657652438653 2316 86-87 0.14442073135206712 2316 88-89 0.3161059396387458 2316 90-91 0.4577681208295772 2316 92-93 0.7573124046380322 2316 94-95 0.9453442919534645 2316 96-97 1.1692327446431037 2316 98-99 1.1444236616173953 2316 100-101 0.7942253848995868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 11.0 4 39.0 5 85.0 6 185.0 7 394.0 8 1037.0 9 1994.0 10 3481.0 11 5567.0 12 8367.0 13 11167.0 14 14167.0 15 19140.0 16 25121.0 17 33878.0 18 30310.0 19 20124.0 20 18139.0 21 18215.0 22 19094.0 23 21385.0 24 25175.0 25 29673.0 26 36289.0 27 45165.0 28 55798.0 29 69682.0 30 86832.0 31 107764.0 32 136747.0 33 176207.0 34 237157.0 35 334991.0 36 504203.0 37 748975.0 38 593156.0 39 52699.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.79624607264584 20.09676370257984 15.701393574970378 22.405596649803936 2 15.612947118074505 21.096247818848926 39.549716500468726 23.74108856260784 3 18.569233614952605 25.217112937669047 30.620583652261956 25.593069795116396 4 13.018701634567645 17.749830919631133 37.41019842390181 31.82126902189942 5 13.40883541588281 37.78107832138437 34.45589994599631 14.354186316736506 6 31.157861220665772 37.23473280147703 17.52150799645633 14.085897981400874 7 27.719044262119525 33.3916691625468 21.132010965456065 17.757275609877617 8 26.21576086738659 35.10509325619871 19.937481781983966 18.74166409443074 9 26.098592896276568 16.905144910895647 20.63702496433707 36.35923722849071 10-11 24.369805887958364 27.24460659898477 27.59559571368359 20.78999179937327 12-13 25.48879885035501 24.498308162299015 28.21601287551989 21.796880111826084 14-15 22.433586204645373 25.884558051690203 26.573735934546285 25.10811980911814 16-17 21.59930844554295 29.067893284373998 27.28998671981794 22.042811550265117 18-19 21.652881581893595 27.365450108835642 29.932751540335044 21.04891676893571 20-21 22.24533894319457 26.502528403286945 30.19546752876192 21.056665124756563 22-23 22.0842603489301 26.341602741961808 30.06183444575205 21.51230246335604 24-25 21.874585706705975 26.608417208487133 30.089110818856867 21.427886265950026 26-27 21.6801671396688 26.89267306502089 29.898864923733864 21.52829487157645 28-29 21.66782707521638 27.00149714894563 29.911495403679716 21.41918037215827 30-31 21.76837147746929 26.714138169877486 29.931600261252182 21.58589009140104 32-33 21.583617329177287 26.760869677261223 29.75828808775114 21.897224905810354 34-35 22.10176777033245 26.783418498680856 29.60302334597594 21.511790385010745 36-37 22.134688229168248 26.617753128846722 29.57816368645132 21.66939495553371 38-39 21.820623481972653 26.860964622491252 29.592952129658595 21.7254597658775 40-41 22.156673904724215 26.77371661680391 29.465145868072263 21.60446361039961 42-43 21.93357600291639 26.805939648756254 29.359969713553095 21.90051463477426 44-45 22.032654204226645 27.00329915067343 29.263483653427112 21.700562991672808 46-47 22.280211268855826 27.271417944337767 28.594740269074016 21.85363051773239 48-49 22.243818186920137 27.340166795421627 28.405816937357514 22.010198080300718 50-51 22.360298955853562 27.462803750520415 28.31370321226931 21.86319408135672 52-53 22.60124921902336 27.418407626569756 28.145352995714724 21.834990158692165 54-55 22.364078567824848 27.480900074504174 28.1928701628841 21.962151194786884 56-57 22.35995535476526 27.693403030905202 28.064077021619955 21.882564592709585 58-59 22.43219748358016 27.3535672378653 28.05604534618096 22.158189932373578 60-61 22.25607329240281 27.428916093186906 28.36610237823493 21.948908236175356 62-63 22.176672807581674 27.495517437468898 28.467550599555263 21.860259155394164 64-65 22.136824589135244 27.383916112048933 28.34512625269494 22.134133046120876 66-67 22.147495348500268 27.555490144089116 28.13790830546824 22.15910620194238 68-69 22.25183554564712 27.436461000417477 28.26528409224315 22.04641936169226 70-71 22.168039830238158 27.371849679105214 28.175403774228887 22.284706716427742 72-73 22.26190019419188 27.392981146035616 27.98703879428087 22.35807986549163 74-75 22.21707456888085 27.749753609386264 27.800091168822092 22.23308065291079 76-77 22.31641823353535 27.49936863300025 27.972292580908036 22.211920552556364 78-79 22.273052912849593 27.6113969044978 27.91280664249624 22.202743540156373 80-81 22.239470693522172 27.702031170944835 27.818025271309082 22.24047286422391 82-83 22.284466763333494 27.709334075514658 27.77012307840321 22.23607608274864 84-85 22.232826189757283 27.63581012331479 27.770545413781655 22.360818273146275 86-87 22.26642427518782 27.549620292164008 27.76550199259998 22.418453440048193 88-89 22.274513217474865 27.510306600977774 27.6737605020241 22.541419679523266 90-91 22.24573210446234 27.489667177670135 27.731924916709584 22.53267580115794 92-93 22.240198125635054 27.282792187850546 27.85234039342508 22.624669293089326 94-95 22.019403726124732 27.357661817500862 27.880350656363976 22.742583800010422 96-97 22.14012224181385 27.271102976673493 27.802310259184026 22.786464522328632 98-99 21.94566880468192 27.34113529225563 27.694129002765568 23.019066900296885 100-101 21.931473407644454 27.243615901720325 27.982431648804795 22.84247904183043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 377.0 2 334.0 3 452.5 4 446.0 5 443.0 6 771.0 7 1711.5 8 2909.5 9 3544.0 10 3468.0 11 3261.5 12 3437.0 13 3823.5 14 4180.5 15 4527.5 16 4771.0 17 5040.5 18 5464.5 19 5945.5 20 6626.5 21 7745.5 22 9165.5 23 11105.5 24 13740.5 25 17326.0 26 22038.5 27 27976.0 28 33205.0 29 38573.5 30 45548.5 31 53039.5 32 61076.5 33 67627.5 34 72849.5 35 78783.5 36 86826.5 37 95011.5 38 100230.0 39 103574.0 40 107067.5 41 112886.5 42 118224.0 43 122227.0 44 128237.0 45 133128.5 46 133298.5 47 133139.0 48 133476.5 49 130487.0 50 126446.5 51 120872.5 52 114601.5 53 112245.0 54 110760.5 55 102579.5 56 90578.0 57 80586.0 58 74038.0 59 66008.5 60 53833.5 61 43370.0 62 35434.5 63 27002.5 64 19436.5 65 14440.5 66 10376.0 67 7837.5 68 6014.5 69 4168.5 70 2989.5 71 2242.5 72 1771.5 73 1473.0 74 1172.5 75 885.5 76 645.5 77 435.5 78 263.0 79 165.0 80 113.5 81 82.0 82 56.0 83 37.0 84 23.0 85 14.5 86 8.0 87 4.5 88 3.0 89 1.5 90 1.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07092499130975581 2 0.0 3 0.0 4 0.0 5 0.0019470728336953552 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7180054414959016E-4 12-13 0.006113236029322917 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4316712012465849E-5 22-23 9.162695687978143E-4 24-25 8.303692967230192E-4 26-27 3.8655122433657786E-4 28-29 0.0033644273229294745 30-31 3.1496766427424865E-4 32-33 0.0 34-35 0.0 36-37 2.8633424024931698E-5 38-39 5.58351768486168E-4 40-41 7.158356006232923E-5 42-43 0.012341005754745562 44-45 2.2906739219945359E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.295013603739754E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.590027207479508E-5 82-83 4.008679363490437E-4 84-85 5.297183444612363E-4 86-87 0.0 88-89 0.0 90-91 0.007831241470818819 92-93 0.0026485917223061815 94-95 0.0 96-97 0.0 98-99 2.433841042119194E-4 100-101 0.009935798136651299 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3492422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.65225840331536 #Duplication Level Percentage of deduplicated Percentage of total 1 71.05264675715304 34.568717302684966 2 15.235802690244856 14.825124189354403 3 5.464848374365349 7.976316457336827 4 2.6991383386450907 5.252767036722251 5 1.504413724138554 3.6596562626141456 6 0.9099481558226725 2.656261968642296 7 0.5827094958541233 1.9845093076452298 8 0.4275106853365311 1.6639488266537081 9 0.31597396107699555 1.3835562122733387 >10 1.6225879559686598 14.328875785545511 >50 0.12315709491816042 4.119765126558101 >100 0.057197384923649855 4.865618873600132 >500 0.002710254370965811 0.9231632973360224 >1k 0.0011783714619611927 0.7997022079980144 >5k 5.8918573098059644E-5 0.23193026614495427 >10k+ 1.1783714619611929E-4 0.7600868788900763 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14816 0.42423281035338806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11700 0.3350110610917008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8091 0.23167303378572235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.590027207479508E-5 2 0.0 0.0 0.0 0.0 8.590027207479508E-5 3 0.0 0.0 0.0 0.0 8.590027207479508E-5 4 0.0 0.0 0.0 0.0 8.590027207479508E-5 5 0.0 0.0 0.0 0.0 8.590027207479508E-5 6 0.0 0.0 0.0 0.0 8.590027207479508E-5 7 2.8633424024931695E-5 0.0 0.0 0.0 8.590027207479508E-5 8 2.8633424024931695E-5 0.0 0.0 0.0 8.590027207479508E-5 9 2.8633424024931695E-5 0.0 0.0 0.0 8.590027207479508E-5 10-11 2.8633424024931695E-5 0.0 0.0 0.0 8.590027207479508E-5 12-13 2.8633424024931695E-5 0.0 0.0 0.0 1.4316712012465846E-4 14-15 2.8633424024931695E-5 0.0 0.0 0.0 1.7180054414959016E-4 16-17 2.8633424024931695E-5 0.0 0.0 1.4316712012465847E-5 2.2906739219945356E-4 18-19 4.295013603739754E-5 0.0 0.0 2.8633424024931695E-5 2.2906739219945356E-4 20-21 5.726684804986339E-5 0.0 0.0 2.8633424024931695E-5 2.2906739219945356E-4 22-23 5.726684804986339E-5 0.0 0.0 1.4316712012465846E-4 2.2906739219945356E-4 24-25 1.1453369609972678E-4 0.0 0.0 2.8633424024931693E-4 2.2906739219945356E-4 26-27 1.1453369609972678E-4 0.0 0.0 4.2950136037397545E-4 2.433841042119194E-4 28-29 1.2885040811219263E-4 0.0 0.0 0.001131020248984802 2.5770081622438526E-4 30-31 1.4316712012465846E-4 0.0 0.0 0.002834708978468238 2.5770081622438526E-4 32-33 1.4316712012465846E-4 0.0 0.0 0.004624297980026469 2.5770081622438526E-4 34-35 1.5748383213712433E-4 0.0 0.0 0.006685904509821551 2.5770081622438526E-4 36-37 1.7180054414959016E-4 0.0 0.0 0.01045119976910007 2.5770081622438526E-4 38-39 2.0043396817452186E-4 0.0 0.0 0.015662482941637638 2.5770081622438526E-4 40-41 2.0043396817452186E-4 0.0 0.0 0.021117150218387126 2.5770081622438526E-4 42-43 2.0043396817452186E-4 0.0 0.0 0.026872468447398396 2.8633424024931693E-4 44-45 2.0043396817452186E-4 0.0 0.0 0.032441669420247614 3.579178003116462E-4 46-47 2.2906739219945356E-4 0.0 0.0 0.03949980844239327 4.008679363490437E-4 48-49 2.2906739219945356E-4 0.0 0.0 0.04655794746453894 4.2950136037397545E-4 50-51 2.2906739219945356E-4 0.0 0.0 0.054933223991831454 4.2950136037397545E-4 52-53 2.2906739219945356E-4 0.0 0.0 0.07201306142270321 4.2950136037397545E-4 54-55 2.2906739219945356E-4 0.0 0.0 0.097883360029229 4.2950136037397545E-4 56-57 2.2906739219945356E-4 0.0 0.0 0.13546472906195184 4.2950136037397545E-4 58-59 2.2906739219945356E-4 0.0 0.0 0.18656107423444246 4.2950136037397545E-4 60-61 2.2906739219945356E-4 0.0 0.0 0.24753595069553452 4.2950136037397545E-4 62-63 2.2906739219945356E-4 0.0 0.0 0.2916886905419792 5.440350564737022E-4 64-65 2.8633424024931693E-4 0.0 0.0 0.34915597256001707 5.440350564737022E-4 66-67 3.1496766427424865E-4 0.0 0.0 0.44317382034588026 5.726684804986339E-4 68-69 3.436010882991803E-4 0.0 0.0 0.564407737667441 6.013019045235656E-4 70-71 3.579178003116462E-4 0.0 0.0 0.6890060823119314 6.013019045235656E-4 72-73 3.7223451232411205E-4 0.0 0.0 0.8269332858400273 6.156186165360315E-4 74-75 3.865512243365779E-4 0.0 0.0 0.9685255676433147 6.442520405609631E-4 76-77 4.008679363490437E-4 0.0 0.0 1.116274035611962 6.58568752573429E-4 78-79 4.008679363490437E-4 0.0 0.0 1.2646667556211706 6.58568752573429E-4 80-81 4.2950136037397545E-4 0.0 0.0 1.4173688059461313 6.58568752573429E-4 82-83 4.581347843989071E-4 0.0 0.0 1.619850063938436 7.158356006232924E-4 84-85 4.581347843989071E-4 0.0 0.0 1.8892476338770057 7.158356006232924E-4 86-87 4.867682084238388E-4 0.0 0.0 2.1986174637543803 7.874191606856216E-4 88-89 4.867682084238388E-4 0.0 0.0 2.5570649824104876 8.590027207479509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7520 0.0 49.73461 1 GTATCAA 13485 0.0 42.994286 1 ATCAACG 17195 0.0 33.617577 3 TATCAAC 17580 0.0 32.989426 2 TCAACGC 17600 0.0 32.817 4 CAACGCA 17965 0.0 32.15025 5 AACGCAG 18555 0.0 31.153555 6 ACGCAGA 21135 0.0 27.285095 7 CGCAGAG 21135 0.0 27.262619 8 GCAGAGT 23100 0.0 24.65564 9 GTACATG 13250 0.0 23.707592 1 TACATGG 13335 0.0 22.867569 2 GAGTACT 14075 0.0 22.54432 12-13 ACATGGG 13540 0.0 21.644346 3 AGTACTT 14580 0.0 21.616852 12-13 AGAGTAC 21265 0.0 21.399773 10-11 GTACTTT 14755 0.0 21.262365 14-15 CAGAGTA 22575 0.0 20.420992 10-11 TACTTTT 17125 0.0 18.250439 14-15 ACTTTTT 18110 0.0 17.690811 16-17 >>END_MODULE