FastQCFastQC Report
Thu 26 May 2016
SRR937519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226463
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148371.2097388995835994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111660.9104228990193752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69020.5627564794045967No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41940.34195895024962025No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30110.24550271797844697No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25540.20824109655162856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22870.18647117768738233No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22250.18141599053538507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21290.17358860397745385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.14635582157798482No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15500.12637967879993117No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14120.11512781062290504No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13200.10762656517155429No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12860.10485436576562032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163800.031.5081461
GGTATCA110050.029.6238141
TATCAAC195900.026.3156092
ATCAACG197350.026.0741063
TCAACGC198400.025.9392954
CCGCTAG559.501992E-525.90062982-83
CAACGCA200200.025.7071275
GAGTACT103100.025.33948712-13
AACGCAG203700.025.2612926
GTACATG109500.024.8249761
GTACTTT107350.024.29204614-15
TACATGG112150.023.979232
AGTACTT104050.022.98535312-13
ACGCAGA225300.022.7129027
ACATGGG111900.022.6315983
CGCAGAG225850.022.6155158
GTAAGCG1800.022.42781494-95
GGACCGC1503.026711E-522.1730236
AGAGTAC164750.021.92999810-11
GCAGAGT227300.021.9268579
Produced by FastQC (version 0.11.5)