##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1226463 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.615027929909015 31.0 31.0 34.0 30.0 34.0 2 31.680151786071004 33.0 31.0 34.0 30.0 34.0 3 31.674278800094253 33.0 31.0 34.0 30.0 34.0 4 35.35584603856782 37.0 35.0 37.0 33.0 37.0 5 35.386421767309734 37.0 35.0 37.0 33.0 37.0 6 35.45178778324336 37.0 35.0 37.0 33.0 37.0 7 35.38692402461387 37.0 35.0 37.0 33.0 37.0 8 35.378136152497056 37.0 35.0 37.0 33.0 37.0 9 36.92576457667292 39.0 37.0 39.0 33.0 39.0 10-11 36.80534594194852 39.0 37.0 39.0 33.0 39.0 12-13 36.64322853604226 39.0 37.0 39.0 32.0 39.0 14-15 37.59927939122501 40.0 37.0 41.0 32.0 41.0 16-17 37.5600246399606 40.0 37.0 41.0 32.0 41.0 18-19 37.59886437666689 40.0 37.0 41.0 32.0 41.0 20-21 37.52789117975838 40.0 37.0 41.0 32.0 41.0 22-23 37.58389409219846 40.0 37.0 41.0 32.0 41.0 24-25 37.519179950801615 40.0 37.0 41.0 32.0 41.0 26-27 37.41307075712842 40.0 37.0 41.0 32.0 41.0 28-29 37.25946237269286 39.5 36.5 41.0 32.0 41.0 30-31 36.905247039657944 39.0 36.0 41.0 31.0 41.0 32-33 36.546597002926305 39.0 35.0 40.0 30.5 41.0 34-35 36.44078500533649 39.0 35.0 40.5 30.0 41.0 36-37 36.22979943137298 39.0 35.0 40.5 30.0 41.0 38-39 36.02649162673477 39.0 35.0 40.0 30.0 41.0 40-41 35.73192668674065 38.5 35.0 40.0 28.5 41.0 42-43 35.33760863556422 38.0 35.0 40.0 27.0 41.0 44-45 34.90290453115993 38.0 34.5 40.0 25.0 41.0 46-47 34.253617516386555 38.0 33.5 40.0 22.5 41.0 48-49 33.70918364434965 38.0 33.0 40.0 18.0 41.0 50-51 32.65357413961938 37.0 32.0 39.5 14.0 40.5 52-53 32.71643294579616 37.0 32.5 39.5 12.0 40.0 54-55 32.97478684640303 38.0 33.0 40.0 8.5 41.0 56-57 32.66523776094346 37.0 32.5 40.0 7.5 41.0 58-59 32.3689654722564 37.0 32.0 40.0 4.0 41.0 60-61 31.98933518581482 36.0 31.0 39.5 2.0 41.0 62-63 31.59545375604482 36.0 31.0 39.0 2.0 41.0 64-65 31.14222972890336 35.0 30.5 39.0 2.0 40.0 66-67 30.670923215783922 35.0 29.5 38.5 2.0 40.0 68-69 30.140967970497275 35.0 29.0 38.0 2.0 40.0 70-71 29.65135638009463 34.5 29.0 37.0 2.0 39.5 72-73 29.148278423401276 34.0 28.0 37.0 2.0 39.0 74-75 28.59378269054998 34.0 27.0 36.0 2.0 39.0 76-77 28.073462468904484 34.0 26.0 36.0 2.0 38.5 78-79 27.507617840896955 33.5 26.0 35.0 2.0 37.0 80-81 26.907137842723344 33.0 25.0 35.0 2.0 37.0 82-83 26.32731562224054 32.5 23.5 35.0 2.0 36.0 84-85 25.73366664954426 32.0 21.0 35.0 2.0 36.0 86-87 25.135102322695424 32.0 19.0 35.0 2.0 35.0 88-89 24.537290566450025 31.0 15.0 34.0 2.0 35.0 90-91 23.9068642918702 31.0 7.0 34.0 2.0 35.0 92-93 23.177148026479394 31.0 2.0 34.0 2.0 35.0 94-95 22.349405974742 30.0 2.0 34.0 2.0 35.0 96-97 21.45370019315707 29.5 2.0 34.0 2.0 35.0 98-99 20.24334284850012 29.0 2.0 34.0 2.0 35.0 100-101 17.368959764787032 22.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19556605306077302 1101 2 0.2329199294115618 1101 3 0.17221708777681144 1101 4 0.08880312067288543 1101 5 0.09484410090993833 1101 6 0.0966609159232803 1101 7 0.15198091815318548 1101 8 0.08906047031256037 1101 9 0.11826071261601356 1101 10-11 0.14104057074233367 1101 12-13 0.13051213603241507 1101 14-15 0.15114407815666198 1101 16-17 0.30541711940642813 1101 18-19 0.32617448693928 1101 20-21 0.40363758846277875 1101 22-23 0.43863394452314 1101 24-25 0.4946373224123519 1101 26-27 0.5269891507001745 1101 28-29 0.5476861104693356 1101 30-31 0.46328103129025777 1101 32-33 0.3106261972810813 1101 34-35 0.4985846503836555 1101 36-37 0.6444185533211879 1101 38-39 0.573176004063285 1101 40-41 0.6473066245424306 1101 42-43 0.7707536825563963 1101 44-45 0.9474661476491377 1101 46-47 0.8183336838518471 1101 48-49 0.8578219026105032 1101 50-51 0.9683174367201417 1101 52-53 0.8844762419578984 1101 54-55 1.0022794942420532 1101 56-57 1.1203119895251277 1101 58-59 1.252595367401014 1101 60-61 1.2632336843757734 1101 62-63 1.1580664339441356 1101 64-65 0.9286675036837941 1101 66-67 1.19336252134946 1101 68-69 1.3617498475058163 1101 70-71 1.1423709882260944 1101 72-73 1.3987935393045667 1101 74-75 1.33720008051014 1101 76-77 1.5372905651231399 1101 78-79 1.7407294645359954 1101 80-81 1.8070888330270947 1101 82-83 1.5037132968241984 1101 84-85 1.6541914474231199 1101 86-87 1.7254335309157973 1101 88-89 1.7360709855049237 1101 90-91 1.693071504837242 1101 92-93 1.8903468607663676 1101 94-95 2.1448523950003135 1101 96-97 2.28596306633143 1101 98-99 2.3049093352983867 1101 100-101 2.26812941429133 1102 1 0.16444355518295595 1102 2 0.1823298256299708 1102 3 -0.009115745816661303 1102 4 0.02405942561004082 1102 5 -0.00528870666967407 1102 6 -0.08036422852207892 1102 7 -0.18898713957019453 1102 8 0.01691908500628614 1102 9 0.16118271896886682 1102 10-11 0.1344382712543961 1102 12-13 0.21224826996270707 1102 14-15 0.31125465415819775 1102 16-17 0.2875137677264803 1102 18-19 0.3675104182353124 1102 20-21 0.4741449784515481 1102 22-23 0.4109296159544513 1102 24-25 0.39470945951906344 1102 26-27 0.3634137687072325 1102 28-29 0.47477885671101916 1102 30-31 0.391410053169281 1102 32-33 0.2335930946495708 1102 34-35 0.3847068573170347 1102 36-37 0.44832713515530287 1102 38-39 0.5406069133160116 1102 40-41 0.4323076685195417 1102 42-43 0.8479301181104901 1102 44-45 0.7749938558284413 1102 46-47 0.6950759276153704 1102 48-49 0.7209838867831238 1102 50-51 0.5734951353153903 1102 52-53 0.6617895069706279 1102 54-55 1.0117552979332842 1102 56-57 0.8577543140685222 1102 58-59 0.9365484123912964 1102 60-61 0.8028166754215285 1102 62-63 0.7719647642653342 1102 64-65 0.7323189419761889 1102 66-67 0.8477367854311701 1102 68-69 0.8585079562984994 1102 70-71 1.188329085380996 1102 72-73 1.319300244718189 1102 74-75 1.2315572986705483 1102 76-77 1.2488777697289635 1102 78-79 1.470441224167967 1102 80-81 1.5467072508370023 1102 82-83 1.6333453034508842 1102 84-85 1.6785084125645504 1102 86-87 1.8100525819577236 1102 88-89 1.9248982055821457 1102 90-91 1.9341861472151791 1102 92-93 2.1783882741402287 1102 94-95 2.181118619431434 1102 96-97 2.161149185201058 1102 98-99 2.4085754063846707 1102 100-101 2.3476727684357463 1103 1 0.3397605380688944 1103 2 0.2957943852138385 1103 3 0.28006163829337183 1103 4 0.10376599922296492 1103 5 0.2584957870699185 1103 6 0.25656676459878014 1103 7 0.17863210617605318 1103 8 0.21393636252388148 1103 9 0.3290823147068238 1103 10-11 0.1779963303673 1103 12-13 0.14155751598137556 1103 14-15 0.33443017091934024 1103 16-17 0.2512131493219485 1103 18-19 0.24102993335201006 1103 20-21 0.22828501145623648 1103 22-23 0.163484631338207 1103 24-25 0.23670487287431996 1103 26-27 0.16123176905891512 1103 28-29 0.09378767584811953 1103 30-31 0.17822250018057417 1103 32-33 0.1927747632603598 1103 34-35 0.3264469342399181 1103 36-37 0.13720324208785684 1103 38-39 0.32027439808291547 1103 40-41 0.48651816051066277 1103 42-43 0.3175627902768454 1103 44-45 0.45768158341674336 1103 46-47 0.6004396437552231 1103 48-49 0.9292388979566724 1103 50-51 0.8718394110490166 1103 52-53 0.6664770727550504 1103 54-55 0.8901229444215133 1103 56-57 0.8591085960514064 1103 58-59 0.8417546586815732 1103 60-61 1.0413370614723867 1103 62-63 1.1214710560423171 1103 64-65 0.8466994271071577 1103 66-67 1.079980460426615 1103 68-69 1.1547314454341127 1103 70-71 1.360594702964125 1103 72-73 1.4270579153366647 1103 74-75 1.3671322349189943 1103 76-77 1.2208615513663688 1103 78-79 1.2370535709599153 1103 80-81 1.1848012919137254 1103 82-83 1.5395650942120476 1103 84-85 1.815491909394101 1103 86-87 1.965297421776004 1103 88-89 1.7150839746004962 1103 90-91 1.8050736662746054 1103 92-93 1.9342070224220969 1103 94-95 2.0979912558358187 1103 96-97 2.0142781090483908 1103 98-99 2.0053306194003078 1103 100-101 1.9993490483993916 1104 1 0.1465707246906227 1104 2 0.022147381508712982 1104 3 0.0179604973207681 1104 4 0.06216499908845918 1104 5 0.13474958346422028 1104 6 0.09798265154564234 1104 7 0.025388394117001667 1104 8 -0.0159363598950506 1104 9 0.003927403013740616 1104 10-11 0.010296169578836611 1104 12-13 0.14843650682015408 1104 14-15 0.2420334252811358 1104 16-17 0.17356985932750746 1104 18-19 0.21738344067047422 1104 20-21 0.007359085757435935 1104 22-23 0.20614937819142654 1104 24-25 0.13244849601527164 1104 26-27 0.16417526497542667 1104 28-29 0.13450113020001453 1104 30-31 0.23871522504423837 1104 32-33 0.24608045226121078 1104 34-35 0.3780263855313919 1104 36-37 0.29432684970186074 1104 38-39 0.30510967064859074 1104 40-41 0.20703199678116135 1104 42-43 0.3150888228408988 1104 44-45 0.331992263687269 1104 46-47 0.4929638182374916 1104 48-49 0.6091347390831174 1104 50-51 0.48003189605865515 1104 52-53 0.4192297524605877 1104 54-55 0.6479092065537273 1104 56-57 0.6715426995586284 1104 58-59 0.686165757132926 1104 60-61 0.5409045311002068 1104 62-63 0.6490462981346354 1104 64-65 0.7342039190045675 1104 66-67 1.0202693972563281 1104 68-69 0.9496088222559642 1104 70-71 0.8755096420644222 1104 72-73 1.050965397989458 1104 74-75 1.0792844986922034 1104 76-77 1.256186977324976 1104 78-79 1.100425893349577 1104 80-81 1.2107390099930484 1104 82-83 1.0638041698562155 1104 84-85 0.9947428387416188 1104 86-87 1.2342591567379095 1104 88-89 1.0846807688063898 1104 90-91 1.2519942585223376 1104 92-93 1.2714184116254366 1104 94-95 1.1924081799561748 1104 96-97 1.3575060856688559 1104 98-99 1.4254805251062308 1104 100-101 1.108596899340757 1105 1 0.10366147207944465 1105 2 0.0034366467367910047 1105 3 0.06493853805904948 1105 4 0.0273343842421383 1105 5 -0.05562539539921829 1105 6 0.0013775898268875153 1105 7 0.1563773575800198 1105 8 0.10226335785056051 1105 9 0.042761363940371666 1105 10-11 0.10710385775927023 1105 12-13 0.06793750511622676 1105 14-15 0.19375833130472842 1105 16-17 0.23077941858142026 1105 18-19 0.2303212520282898 1105 20-21 0.06953533053832928 1105 22-23 0.03953494322049522 1105 24-25 -0.025010962433498207 1105 26-27 0.03665046203321509 1105 28-29 0.13153733713021154 1105 30-31 0.14025807210658314 1105 32-33 0.2599450205550653 1105 34-35 0.42187763779138265 1105 36-37 0.4173845216234042 1105 38-39 0.4557883575153099 1105 40-41 0.285394173391758 1105 42-43 0.41460138121032486 1105 44-45 0.3339660933721049 1105 46-47 0.41426833430777776 1105 48-49 0.5365290237568274 1105 50-51 0.5517683702850533 1105 52-53 0.5292398654924213 1105 54-55 0.7183563505164798 1105 56-57 0.8393944363336416 1105 58-59 0.6341289648825637 1105 60-61 0.5169041685268745 1105 62-63 0.4826566423679317 1105 64-65 0.6673914507094132 1105 66-67 0.7403325061518942 1105 68-69 0.6482240116395559 1105 70-71 0.8601236478005596 1105 72-73 1.1476062292741709 1105 74-75 0.9654295637928136 1105 76-77 0.8768137414149351 1105 78-79 0.8561020763466765 1105 80-81 0.736827217742686 1105 82-83 0.8853418791081715 1105 84-85 0.8266974178964155 1105 86-87 0.6230466985232646 1105 88-89 1.0354970059130864 1105 90-91 0.9655625491272986 1105 92-93 1.033298988425944 1105 94-95 1.237197976850073 1105 96-97 1.3463394775342081 1105 98-99 1.4671569471090677 1105 100-101 1.4511786978345356 1106 1 -0.0016215132076915495 1106 2 -0.6241381040937739 1106 3 -0.07682997167042416 1106 4 -0.06695251656469736 1106 5 -0.2257220968705056 1106 6 -0.13092841396998267 1106 7 -0.09886113316511569 1106 8 -0.0427947816843286 1106 9 -0.002009044222894829 1106 10-11 -0.5163848109640341 1106 12-13 -0.28095606393930694 1106 14-15 -0.12574214330371802 1106 16-17 -0.12470675844753032 1106 18-19 -0.10232735974199869 1106 20-21 -0.25575685028749007 1106 22-23 -0.06434349769074998 1106 24-25 -0.271120656311048 1106 26-27 -0.132083223301386 1106 28-29 0.11893796598639739 1106 30-31 -0.029970629839304763 1106 32-33 0.02470979274301044 1106 34-35 0.08537579868364986 1106 36-37 0.07710657905102636 1106 38-39 0.3233206902158301 1106 40-41 0.4414513637097457 1106 42-43 0.40663982449461145 1106 44-45 0.4034725005861546 1106 46-47 0.39877830108518 1106 48-49 0.6030691281706879 1106 50-51 0.4779712060705279 1106 52-53 0.4526083989523144 1106 54-55 0.6741628882478494 1106 56-57 0.5617470110844707 1106 58-59 0.23733108875536857 1106 60-61 0.49938666734035664 1106 62-63 0.5659667396626631 1106 64-65 0.7253900862101226 1106 66-67 0.7955339181120245 1106 68-69 0.8216563206096552 1106 70-71 0.9296523022856107 1106 72-73 0.9573446013671933 1106 74-75 0.7290715903708858 1106 76-77 0.9343511168302179 1106 78-79 0.8533271000770988 1106 80-81 0.6582498565656572 1106 82-83 0.7850348667683527 1106 84-85 0.7401288369756749 1106 86-87 0.6937989529133937 1106 88-89 0.8021631781959542 1106 90-91 0.8553199055582397 1106 92-93 0.9310460814491996 1106 94-95 0.8839335606184662 1106 96-97 0.7630931557496758 1106 98-99 1.0137279010720484 1106 100-101 0.8154199175782466 1107 1 0.14928397365739698 1107 2 0.03390424614768861 1107 3 0.09479468154737347 1107 4 0.13404330492381433 1107 5 0.0834380908200103 1107 6 0.14430705542756073 1107 7 0.1566009006054756 1107 8 0.054767039735679646 1107 9 0.2682045051143689 1107 10-11 0.03478440162630392 1107 12-13 0.10476293116293078 1107 14-15 0.10909469781010017 1107 16-17 0.19320775331737394 1107 18-19 0.16510914914590558 1107 20-21 0.0736412899323966 1107 22-23 0.14572774250515863 1107 24-25 0.13809326121472765 1107 26-27 0.18911009279273117 1107 28-29 0.21342969458865468 1107 30-31 0.1697887632490236 1107 32-33 0.07376984172064738 1107 34-35 0.16049995527507832 1107 36-37 0.1865467612615319 1107 38-39 0.31348021002951043 1107 40-41 0.28294834970146354 1107 42-43 0.2433148253508719 1107 44-45 0.44772319415056216 1107 46-47 0.6291063313622729 1107 48-49 0.7019968861595203 1107 50-51 0.6934387626688121 1107 52-53 0.6753055082443638 1107 54-55 0.6220323183262835 1107 56-57 0.5808570651996163 1107 58-59 0.5917116758019816 1107 60-61 0.6757282007170708 1107 62-63 0.5915188083683489 1107 64-65 0.5905485357083613 1107 66-67 0.8047424840605828 1107 68-69 0.9526928997102466 1107 70-71 0.9179522560479825 1107 72-73 1.0493919435598862 1107 74-75 0.9040801528944371 1107 76-77 1.0805600595298088 1107 78-79 1.0577740466812031 1107 80-81 1.0164137946414868 1107 82-83 1.203887402987828 1107 84-85 1.082424064225048 1107 86-87 1.19496837956682 1107 88-89 1.1014060370513157 1107 90-91 1.0766568579848617 1107 92-93 1.1520167719310983 1107 94-95 1.2542102585668857 1107 96-97 1.522675475851063 1107 98-99 1.5936034019796743 1107 100-101 1.8227466549142726 1108 1 0.09405954991522947 1108 2 0.10941671318197521 1108 3 0.11120989543921311 1108 4 0.021419576458562517 1108 5 0.04404534498996071 1108 6 -0.009998181411845053 1108 7 0.006786707366444489 1108 8 -0.14046920028897603 1108 9 0.1043032481359063 1108 10-11 0.11584110265723524 1108 12-13 0.09877815532979639 1108 14-15 0.04236416898863382 1108 16-17 0.08858227809732 1108 18-19 0.11361948411182254 1108 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1.8887673543733818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5060.0 3 880.0 4 832.0 5 994.0 6 1145.0 7 1442.0 8 1835.0 9 2445.0 10 3258.0 11 4442.0 12 6324.0 13 8629.0 14 10057.0 15 13804.0 16 18099.0 17 23854.0 18 19810.0 19 12364.0 20 11990.0 21 12282.0 22 13554.0 23 14905.0 24 16629.0 25 18922.0 26 21244.0 27 24926.0 28 28663.0 29 33845.0 30 40545.0 31 48550.0 32 59381.0 33 75419.0 34 97318.0 35 127256.0 36 158183.0 37 175568.0 38 103628.0 39 8381.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.060982543343044 19.368389535658977 15.800078370482959 19.770549550515014 2 15.14531558294268 21.646514723715185 40.06724673517264 23.140922958169487 3 18.900194440219853 27.75352044729427 30.79743384698912 22.548851265496754 4 11.992098099995339 17.584834631646686 38.25700308340904 32.16606418494894 5 12.375149942721267 39.21880148851113 32.729828360045694 15.676220208721903 6 28.63078136558134 37.48674837649732 17.748061809577134 16.13440844834421 7 26.199772931432324 36.14795912111877 20.507937182600564 17.14433076484835 8 27.259344268459053 32.38580771409445 19.82970368572099 20.525144331725514 9 28.170076819381666 16.31813050220763 20.620817014221846 34.89097566418886 10-11 24.807387301950655 27.676752399483313 25.852899818505865 21.66296048006017 12-13 26.988531511062007 25.816464121683126 27.106408563334593 20.08859580392027 14-15 23.676669162386343 25.747898527743356 27.998460064670745 22.576972245199553 16-17 20.416108150521463 29.728765488606268 27.85627881125969 21.99884754961258 18-19 20.385596697056968 26.881319281224155 32.633683546414126 20.099400475304748 20-21 22.195519729446218 25.15997317673077 32.721568826163235 19.922938267659777 22-23 21.595148129245377 25.45630329259188 32.921587384220715 20.02696119394203 24-25 21.07031693434397 25.67654872192821 33.10821551322899 20.144918830498824 26-27 20.400510444874037 25.905976315717428 33.28728215073325 20.40623108867529 28-29 20.26489205528182 26.650272518317742 32.86056772742283 20.22426769897761 30-31 20.353768157350558 26.05592322951398 32.83849609701524 20.751812516120225 32-33 20.224624957493003 26.312853135054542 32.65011509586962 20.81240681158283 34-35 21.008375198426645 26.244917743878815 32.44551543518172 20.30119162251282 36-37 20.905975885594493 25.873518901059832 32.57798564414395 20.642519569201724 38-39 20.496576419913396 26.500283153240247 32.31305552990515 20.69008489694121 40-41 20.55434640051546 26.630633074718972 32.1813288575303 20.63369166723527 42-43 20.474309754059338 26.743559305064903 31.452943952826466 21.32918698804929 44-45 20.620711467360568 26.72056174669748 31.397616237827027 21.261110548114925 46-47 21.18383792644456 27.22387488766348 30.64405455253589 20.94823263335607 48-49 21.08903230869144 27.457830640840204 29.94060262201843 21.512534428449925 50-51 20.95119711024173 27.968515794219584 29.76100306037402 21.319284035164664 52-53 21.370751127758687 28.208766795775308 28.93730258601587 21.48317949045013 54-55 20.779003684532118 27.984379055182153 29.324952027514446 21.911665232771288 56-57 21.334297041337436 28.029561702167648 28.901883602432044 21.734257654062876 58-59 21.70157570706637 27.583616241589354 28.95791714158486 21.756890909759417 60-61 21.291896131910512 27.827815272988744 29.821670779337506 21.05861781576324 62-63 21.238404740736865 27.938241965849958 29.70130971681092 21.122043576602252 64-65 20.926691622582556 28.226317447257376 29.47201610600286 21.374974824157206 66-67 21.210688772539797 28.061194264620866 29.069645348635305 21.658471614204032 68-69 21.106423591108804 27.919411153791785 29.370853648870703 21.603311606228708 70-71 21.379491239983036 28.09276686776178 28.981924319271386 21.5458175729838 72-73 21.179470983088603 28.103946809853724 29.111987190303985 21.604595016753684 74-75 20.645607055972008 28.874128565719893 28.828284033436276 21.651980344871827 76-77 20.947379820315355 28.184743764468863 29.03090028258342 21.83697613263236 78-79 20.861360162807614 28.44683007363939 28.860877951500985 21.83093181205201 80-81 21.110475497787384 28.536037232412465 28.496729088435153 21.856758181365 82-83 20.94545317860553 28.269134205519197 28.8937136348055 21.891698981069773 84-85 20.819268367113082 28.41665825798542 28.61164434178818 22.152429033113318 86-87 20.86487736383621 28.359906510785944 28.388789955261302 22.386426170116543 88-89 20.85836755556627 28.066752659773325 28.19345425484058 22.88142552981982 90-91 20.791798465562593 27.884587884497996 28.58761974258256 22.73599390735685 92-93 21.361351954302897 27.76384927801522 28.706678869993706 22.168119897688175 94-95 20.932089196718003 27.597532863690553 28.7212353331966 22.749142606394845 96-97 20.548986341537564 27.55899012533946 29.04885190624344 22.843171626879542 98-99 20.668833235874494 27.906980545418065 28.447233497072354 22.976952721635087 100-101 21.105277994533665 26.74614231261056 29.284065514297037 22.864514178558736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2870.0 1 1760.5 2 781.5 3 955.0 4 895.0 5 929.5 6 1557.0 7 3034.5 8 5112.5 9 6151.0 10 5245.0 11 4123.0 12 3955.5 13 4098.0 14 4235.5 15 4369.0 16 4375.0 17 4399.0 18 4520.0 19 4635.0 20 4829.0 21 5180.0 22 5831.0 23 6519.0 24 7111.5 25 8091.5 26 9383.5 27 10939.0 28 12361.0 29 14099.0 30 16230.5 31 18206.5 32 20847.5 33 22805.5 34 24161.5 35 25399.5 36 27539.5 37 30301.5 38 32057.5 39 32939.5 40 33785.5 41 36143.5 42 37981.5 43 39291.5 44 41606.0 45 43733.0 46 43618.5 47 45808.0 48 51829.0 49 50828.5 50 43880.0 51 38318.5 52 34779.5 53 33642.0 54 33007.0 55 30822.5 56 27729.5 57 25111.0 58 23543.5 59 21480.0 60 18316.0 61 16052.5 62 13857.0 63 10274.5 64 7132.0 65 5362.5 66 4021.5 67 3132.5 68 2475.0 69 1857.5 70 1316.0 71 976.5 72 815.5 73 691.5 74 549.5 75 418.5 76 297.5 77 204.0 78 128.5 79 79.5 80 54.5 81 35.5 82 19.0 83 14.5 84 11.0 85 6.5 86 3.5 87 5.0 88 5.0 89 2.5 90 3.0 91 2.5 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3313593642857551 2 0.28569960936448957 3 0.28251973357533006 4 0.2826012688519751 5 0.2850473271513287 6 0.24688881768141396 7 0.24819338210773584 8 0.2499871581939284 9 0.26123902637095453 10-11 0.2676803132259188 12-13 0.25720303017702123 14-15 0.24745956461792976 16-17 0.24321973023238372 18-19 0.23050022707574547 20-21 0.23632999935587132 22-23 0.23257937663019596 24-25 0.23494389965290435 26-27 0.23029638888413267 28-29 0.2494164112574126 30-31 0.26356278175534037 32-33 0.2556130922824415 34-35 0.2518624695567661 36-37 0.2381237754420639 38-39 0.22373279911420074 40-41 0.2199414087502028 42-43 0.23608539352593597 44-45 0.23245707371522825 46-47 0.24611423255328532 48-49 0.252147843025024 50-51 0.26470427562837195 52-53 0.2822751277453947 54-55 0.25320780162141054 56-57 0.22157211428310516 58-59 0.20938259042466018 60-61 0.21223632510723928 62-63 0.21908528834542906 64-65 0.20966796389291809 66-67 0.21651692713110793 68-69 0.2157423420029793 70-71 0.21720997698259142 72-73 0.2203490851334284 74-75 0.22520043409381285 76-77 0.23893912820851507 78-79 0.23914296640012786 80-81 0.22813570405303707 82-83 0.2184737737705907 84-85 0.2062434822738232 86-87 0.2102387108294339 88-89 0.22189825538968563 90-91 0.21949296472865468 92-93 0.22446661660400682 94-95 0.21981910583523515 96-97 0.23041869179910032 98-99 0.23396547633316292 100-101 0.21264400149046486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1226463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.88487777964394 #Duplication Level Percentage of deduplicated Percentage of total 1 71.72901111085703 32.91276908076397 2 14.124935160331114 12.96241846954377 3 5.517265280041843 7.594771291577788 4 2.7018443310659257 4.958951876423353 5 1.5480351866482942 3.5515702668972637 6 0.9871223919453448 2.7176394184777157 7 0.6585012831907506 2.115067562786241 8 0.44804838695042687 1.6446916379669563 9 0.3393735042868129 1.4014900589264891 >10 1.7511763246194894 14.593063589998279 >50 0.12619707388603732 4.008480670224129 >100 0.060484649152003835 5.156073608507433 >500 0.005158982082797308 1.5622655040892408 >1k 0.0023126471405643108 2.1347996096162762 >5k 1.7789593388956235E-4 0.5633918443609353 >10k+ 3.557918677791247E-4 2.1225555098402493 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14837 1.2097388995835994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11166 0.9104228990193752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6902 0.5627564794045967 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4194 0.34195895024962025 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3011 0.24550271797844697 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2554 0.20824109655162856 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2287 0.18647117768738233 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2225 0.18141599053538507 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2129 0.17358860397745385 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.14635582157798482 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1550 0.12637967879993117 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1412 0.11512781062290504 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1320 0.10762656517155429 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1286 0.10485436576562032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.153527664511689E-5 2 0.0 0.0 0.0 0.0 8.153527664511689E-5 3 0.0 0.0 0.0 0.0 8.153527664511689E-5 4 0.0 0.0 0.0 0.0 8.153527664511689E-5 5 0.0 0.0 0.0 0.0 8.153527664511689E-5 6 0.0 0.0 0.0 0.0 8.153527664511689E-5 7 0.0 0.0 0.0 0.0 8.153527664511689E-5 8 0.0 0.0 0.0 0.0 8.153527664511689E-5 9 0.0 0.0 0.0 0.0 8.153527664511689E-5 10-11 0.0 0.0 0.0 0.0 8.153527664511689E-5 12-13 0.0 0.0 0.0 0.0 2.446058299353507E-4 14-15 0.0 0.0 0.0 8.153527664511689E-5 2.446058299353507E-4 16-17 0.0 0.0 0.0 8.153527664511689E-5 2.446058299353507E-4 18-19 0.0 0.0 0.0 8.153527664511689E-5 2.446058299353507E-4 20-21 0.0 0.0 0.0 8.153527664511689E-5 2.446058299353507E-4 22-23 0.0 0.0 0.0 4.892116598707013E-4 2.446058299353507E-4 24-25 0.0 0.0 0.0 6.522822131609351E-4 2.446058299353507E-4 26-27 0.0 0.0 0.0 0.0013045644263218704 2.446058299353507E-4 28-29 0.0 0.0 0.0 0.0028537346825790915 2.446058299353507E-4 30-31 0.0 0.0 0.0 0.005625934088513066 2.446058299353507E-4 32-33 0.0 0.0 0.0 0.010191909580639612 2.446058299353507E-4 34-35 0.0 0.0 0.0 0.013860997029669872 2.446058299353507E-4 36-37 0.0 0.0 0.0 0.015817843669152676 2.446058299353507E-4 38-39 0.0 0.0 0.0 0.017896993223603157 2.446058299353507E-4 40-41 0.0 0.0 0.0 0.02091379845947248 2.446058299353507E-4 42-43 0.0 0.0 0.0 0.02474595646179298 2.446058299353507E-4 44-45 0.0 0.0 0.0 0.030575728741918835 2.446058299353507E-4 46-47 0.0 0.0 0.0 0.036364733383722134 2.446058299353507E-4 48-49 0.0 0.0 0.0 0.04353983772849242 2.446058299353507E-4 50-51 0.0 0.0 0.0 0.05556629103364716 2.446058299353507E-4 52-53 0.0 0.0 0.0 0.08667199907375925 2.446058299353507E-4 54-55 0.0 0.0 0.0 0.1265427493532214 2.446058299353507E-4 56-57 0.0 0.0 0.0 0.17750229725641947 2.446058299353507E-4 58-59 0.0 0.0 0.0 0.25182170191844355 2.446058299353507E-4 60-61 0.0 0.0 0.0 0.3676833300311546 2.446058299353507E-4 62-63 0.0 0.0 0.0 0.4456310545038864 2.446058299353507E-4 64-65 0.0 0.0 0.0 0.5271663311490032 2.446058299353507E-4 66-67 0.0 0.0 0.0 0.6722175883006662 2.446058299353507E-4 68-69 0.0 0.0 0.0 0.8649669822897226 2.446058299353507E-4 70-71 0.0 0.0 0.0 1.0401862918000788 2.446058299353507E-4 72-73 0.0 0.0 0.0 1.2001177369394755 2.446058299353507E-4 74-75 0.0 0.0 0.0 1.457116928924884 2.446058299353507E-4 76-77 0.0 0.0 0.0 1.664216531603481 2.446058299353507E-4 78-79 0.0 0.0 0.0 1.8440425842442862 2.446058299353507E-4 80-81 0.0 0.0 0.0 2.0555450918617195 2.446058299353507E-4 82-83 0.0 0.0 0.0 2.4244922186808733 2.446058299353507E-4 84-85 0.0 0.0 0.0 2.8007367527597653 2.446058299353507E-4 86-87 0.0 0.0 0.0 3.207475480303931 2.446058299353507E-4 88-89 0.0 0.0 0.0 3.713972618823397 2.446058299353507E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16380 0.0 31.508146 1 GGTATCA 11005 0.0 29.623814 1 TATCAAC 19590 0.0 26.315609 2 ATCAACG 19735 0.0 26.074106 3 TCAACGC 19840 0.0 25.939295 4 CCGCTAG 55 9.501992E-5 25.900629 82-83 CAACGCA 20020 0.0 25.707127 5 GAGTACT 10310 0.0 25.339487 12-13 AACGCAG 20370 0.0 25.261292 6 GTACATG 10950 0.0 24.824976 1 GTACTTT 10735 0.0 24.292046 14-15 TACATGG 11215 0.0 23.97923 2 AGTACTT 10405 0.0 22.985353 12-13 ACGCAGA 22530 0.0 22.712902 7 ACATGGG 11190 0.0 22.631598 3 CGCAGAG 22585 0.0 22.615515 8 GTAAGCG 180 0.0 22.427814 94-95 GGACCGC 150 3.026711E-5 22.173023 6 AGAGTAC 16475 0.0 21.929998 10-11 GCAGAGT 22730 0.0 21.926857 9 >>END_MODULE