FastQCFastQC Report
Thu 26 May 2016
SRR937519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226463
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173421.413984767579617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140521.1457337074171827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90080.7344697720192129No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49730.40547493075616636No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.27159400650488436No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25960.21166557817072343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.20620271463550063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24450.19935375139731082No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20960.170897939848165No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14510.1183076864120646No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14030.114393993133099No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13670.1114587231738748No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12490.101837560529751No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.10061453138007426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG905.7021316E-736.941352
GGTATCA120600.030.4630131
GTATCAA173550.029.6307951
GAGTACT121000.024.23966212-13
TATCAAC212750.024.1777672
ATCAACG213800.024.0145953
TCAACGC215400.023.8582654
CAACGCA216950.023.7097025
GTACATG102600.023.6245461
AGTACTT121100.023.41559412-13
AACGCAG219950.023.4079076
GTACTTT125400.023.29350714-15
TAAGGTG5550.023.1061735
GGACCGA2903.6379788E-1222.9291156
TACATGG105000.022.8433272
GTAAGGT5400.021.9889014
AGAGTAC182650.021.9481410-11
CGTCCGC652.944492E-421.92124246-47
ACATGGG103100.021.6519223
CATGGGG34300.021.601694
Produced by FastQC (version 0.11.5)