##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1226463 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09448307857636 31.0 31.0 34.0 31.0 34.0 2 32.27714982025548 33.0 31.0 34.0 30.0 34.0 3 32.29830822454489 34.0 31.0 34.0 30.0 34.0 4 35.9959485121035 37.0 35.0 37.0 35.0 37.0 5 35.9279790747866 37.0 35.0 37.0 35.0 37.0 6 35.991449395538226 37.0 35.0 37.0 35.0 37.0 7 35.924920686559645 37.0 35.0 37.0 35.0 37.0 8 35.90791976602637 37.0 35.0 37.0 35.0 37.0 9 37.548629677373064 39.0 37.0 39.0 35.0 39.0 10-11 37.46807323172407 39.0 37.0 39.0 35.0 39.0 12-13 37.34324150015125 39.0 37.0 39.0 34.0 39.0 14-15 38.41811819842914 40.0 38.0 41.0 34.0 41.0 16-17 38.399689187525425 40.0 38.0 41.0 33.5 41.0 18-19 38.50254634668963 40.0 38.0 41.0 34.0 41.0 20-21 38.454070363313036 40.0 38.0 41.0 34.0 41.0 22-23 38.3910961847198 40.0 38.0 41.0 34.0 41.0 24-25 38.37344175894421 40.0 38.0 41.0 34.0 41.0 26-27 38.178206354370246 40.0 37.5 41.0 33.5 41.0 28-29 38.00415626072699 40.0 37.0 41.0 33.0 41.0 30-31 37.89199959558503 40.0 37.0 41.0 33.0 41.0 32-33 37.6371712803403 40.0 36.0 41.0 33.0 41.0 34-35 37.42052185838464 40.0 35.0 41.0 33.0 41.0 36-37 37.173268170340236 39.5 35.0 41.0 32.5 41.0 38-39 37.0002380830078 39.0 35.0 41.0 32.0 41.0 40-41 36.742974309049686 39.0 35.0 41.0 31.0 41.0 42-43 36.40901152338065 39.0 35.0 41.0 30.5 41.0 44-45 35.90028643342686 39.0 35.0 41.0 29.5 41.0 46-47 35.26170581582974 39.0 35.0 40.0 26.5 41.0 48-49 34.69127605154008 38.5 35.0 40.0 23.5 41.0 50-51 34.32488342493822 38.0 34.0 40.0 22.5 41.0 52-53 34.057144813989495 38.0 34.0 40.0 20.5 41.0 54-55 33.81465115539564 38.0 34.0 40.0 19.5 41.0 56-57 33.48358164901836 38.0 33.0 40.0 17.5 41.0 58-59 33.19281584523952 37.0 33.0 40.0 14.5 41.0 60-61 32.80739655415614 37.0 33.0 40.0 10.5 41.0 62-63 32.42450363361961 36.0 32.0 39.0 7.5 41.0 64-65 32.005513415406746 36.0 31.5 39.0 6.0 40.0 66-67 31.54513589076882 35.0 31.0 39.0 2.0 40.0 68-69 31.087207685841317 35.0 31.0 38.0 2.0 40.0 70-71 30.634645725146214 35.0 30.0 37.5 2.0 39.5 72-73 30.148746028212834 34.5 29.5 37.0 2.0 39.0 74-75 29.678161509968092 34.0 29.0 36.0 2.0 39.0 76-77 28.400176360803385 32.5 28.0 35.0 2.0 37.0 78-79 29.02309201337505 34.0 29.0 35.0 2.0 37.0 80-81 28.824226250608454 34.0 29.0 35.0 2.0 37.0 82-83 28.418271077072852 34.0 28.5 35.0 2.0 36.5 84-85 27.99278086660584 34.0 27.5 35.0 2.0 36.0 86-87 27.641273727784693 33.5 27.0 35.0 2.0 36.0 88-89 27.19006402965275 33.0 26.0 35.0 2.0 35.0 90-91 26.616097672738597 33.0 25.0 35.0 2.0 35.0 92-93 26.10892297607021 32.5 24.5 35.0 2.0 35.0 94-95 25.598704975201045 32.0 22.0 34.0 2.0 35.0 96-97 24.932530373928934 32.0 18.5 34.0 2.0 35.0 98-99 23.981891830409886 31.5 2.0 34.0 2.0 35.0 100-101 21.264475976853767 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12943089220450332 1101 2 0.2079743408512087 1101 3 0.16473382432108963 1101 4 0.11500041395948557 1101 5 0.1051103372867388 1101 6 0.15824330938750109 1101 7 0.08857193110210204 1101 8 0.08191573306675792 1101 9 0.17714276695345887 1101 10-11 0.16395287421260463 1101 12-13 0.22952672837814703 1101 14-15 0.2724021947230426 1101 16-17 0.2112534736366456 1101 18-19 0.06580477334582469 1101 20-21 0.08973640582100018 1101 22-23 0.14628064750879588 1101 24-25 0.17790547217592945 1101 26-27 0.05149445052180113 1101 28-29 -0.01838216319542596 1101 30-31 0.180454606072459 1101 32-33 0.06876592519173386 1101 34-35 0.14954904188260087 1101 36-37 0.39775754679101283 1101 38-39 0.20377847857384523 1101 40-41 0.19319271435432483 1101 42-43 0.3040392215564367 1101 44-45 0.26158860812913076 1101 46-47 0.36280907744303903 1101 48-49 0.3886821489970629 1101 50-51 0.48046381670040716 1101 52-53 0.5700554124044004 1101 54-55 0.4537562903756296 1101 56-57 0.4443567586376176 1101 58-59 0.6499870791789633 1101 60-61 0.4168744281268104 1101 62-63 0.5789976511647481 1101 64-65 0.5074879864886164 1101 66-67 0.3845130047122858 1101 68-69 0.5829798158393267 1101 70-71 0.39183743450811903 1101 72-73 0.45501673275834165 1101 74-75 0.5579670429543029 1101 76-77 0.4408118280146702 1101 78-79 0.3504478178754731 1101 80-81 0.3726229027495087 1101 82-83 0.29494523915282045 1101 84-85 0.522241101373158 1101 86-87 0.3958760063130633 1101 88-89 0.7824169072748539 1101 90-91 0.9286358401460149 1101 92-93 1.0162159198269052 1101 94-95 1.0628160591079094 1101 96-97 1.1251631554487993 1101 98-99 1.3912837456791323 1101 100-101 2.306226439621952 1102 1 0.21385355574950893 1102 2 0.20174812983776036 1102 3 0.20752062708130126 1102 4 0.1694480987263205 1102 5 0.08732935341532766 1102 6 0.14160984461573634 1102 7 0.17718077170562196 1102 8 0.09381963925257963 1102 9 0.1911029657540837 1102 10-11 0.08277509689121842 1102 12-13 0.18535580023115017 1102 14-15 0.2861985233465063 1102 16-17 0.31026315010642236 1102 18-19 0.31244094218943275 1102 20-21 0.26089773622435075 1102 22-23 0.16714179170769938 1102 24-25 0.06342910371020594 1102 26-27 0.09153781835771468 1102 28-29 0.015311770099572186 1102 30-31 0.18821212613051586 1102 32-33 0.31293377275054013 1102 34-35 0.4066711906270868 1102 36-37 0.4677485103989909 1102 38-39 0.37120759081678756 1102 40-41 0.4946599300502683 1102 42-43 0.48704969213339666 1102 44-45 0.4433077988243923 1102 46-47 0.3182569265079067 1102 48-49 0.5370680919308839 1102 50-51 0.6692067484619528 1102 52-53 0.5641890312055722 1102 54-55 0.5497380053732996 1102 56-57 0.8450916500639991 1102 58-59 0.8620500635158805 1102 60-61 0.7204638267959851 1102 62-63 0.9085658211584402 1102 64-65 1.0762895007688478 1102 66-67 0.9716581380675251 1102 68-69 0.9620799931442896 1102 70-71 0.7276373776883815 1102 72-73 0.9896874692242896 1102 74-75 0.9631242898668724 1102 76-77 0.9195827246712049 1102 78-79 0.9528812258275003 1102 80-81 1.0221743348807273 1102 82-83 1.1210528817498258 1102 84-85 1.2176261235533907 1102 86-87 1.3314061413112839 1102 88-89 1.493188183870597 1102 90-91 1.6657341656751896 1102 92-93 1.5937132499838143 1102 94-95 1.4203572534177198 1102 96-97 1.654305519697548 1102 98-99 1.9436764214095703 1102 100-101 2.4965404713588555 1103 1 0.09749510071240763 1103 2 0.11121803225185545 1103 3 0.04821025834181114 1103 4 0.056099113343982765 1103 5 0.06681988582440113 1103 6 0.0738730433959347 1103 7 0.0974032025117566 1103 8 -0.02164517954771128 1103 9 0.05922330821694999 1103 10-11 0.1448603196563667 1103 12-13 0.1359053142829083 1103 14-15 0.10696501970632966 1103 16-17 0.07972502011351423 1103 18-19 0.09053207740051761 1103 20-21 0.20451073435947364 1103 22-23 0.12309914647840259 1103 24-25 0.13266114616225622 1103 26-27 0.10325848707005036 1103 28-29 0.054803474761257576 1103 30-31 0.04566352511493932 1103 32-33 0.035847720327730315 1103 34-35 0.24257316058935174 1103 36-37 0.12597599014667793 1103 38-39 0.33078510106781067 1103 40-41 0.30430756907571777 1103 42-43 0.3934158080889958 1103 44-45 0.49031265211532116 1103 46-47 0.8055881365780522 1103 48-49 0.7584069282472612 1103 50-51 0.6106351878057268 1103 52-53 0.7473305939173827 1103 54-55 0.6659571522210541 1103 56-57 0.5682606864145683 1103 58-59 0.6531624899714785 1103 60-61 0.7144085641659998 1103 62-63 0.8569410955439807 1103 64-65 0.6607973569251975 1103 66-67 0.7459087012853729 1103 68-69 0.7295056933794832 1103 70-71 0.8097119841375893 1103 72-73 0.5958545498942591 1103 74-75 0.6497411275420788 1103 76-77 0.6765468958431988 1103 78-79 0.6403770465818184 1103 80-81 0.7336191940164163 1103 82-83 0.846323304743148 1103 84-85 0.8974438960587854 1103 86-87 0.9600486892931492 1103 88-89 1.2048623339668119 1103 90-91 1.1942201106139905 1103 92-93 1.1894192143555884 1103 94-95 1.239551734254281 1103 96-97 1.3283485033941531 1103 98-99 1.5026436173235282 1103 100-101 1.9982231079035842 1104 1 0.02761487237889071 1104 2 0.10843438451636445 1104 3 0.060870152514951315 1104 4 0.004289905843464226 1104 5 -0.1070127625329036 1104 6 -0.05711865717203324 1104 7 -0.023660331621144337 1104 8 0.007121769571917014 1104 9 -0.06944589230911902 1104 10-11 -0.05497341370624298 1104 12-13 -0.03885065150875988 1104 14-15 -0.07503480777209859 1104 16-17 -0.05667555861048612 1104 18-19 0.07288214001112436 1104 20-21 0.1100232889827879 1104 22-23 -0.01385722150928359 1104 24-25 -0.20279252627815225 1104 26-27 -0.021070487845229025 1104 28-29 -0.1792690439841067 1104 30-31 -0.11784256281136862 1104 32-33 0.04369765600008435 1104 34-35 0.003875469475723037 1104 36-37 0.039787976797924784 1104 38-39 0.030827740876709697 1104 40-41 0.10219467047220121 1104 42-43 0.028206049255345533 1104 44-45 0.04964412695804299 1104 46-47 0.17883568335817301 1104 48-49 0.4043094797267557 1104 50-51 0.30691281016419936 1104 52-53 0.11584810628721698 1104 54-55 0.3155486808398109 1104 56-57 0.36342280558444173 1104 58-59 0.2852704704839013 1104 60-61 0.5105718807663777 1104 62-63 0.45576222542941736 1104 64-65 0.3138568960800363 1104 66-67 0.08202772644858669 1104 68-69 0.47498630289572574 1104 70-71 0.2941529508961196 1104 72-73 0.44318870331825977 1104 74-75 0.34636645035568847 1104 76-77 0.41819421515650035 1104 78-79 0.35276519884722646 1104 80-81 0.5316884242227324 1104 82-83 0.47961869968466786 1104 84-85 0.6430612554894815 1104 86-87 0.6042940934535359 1104 88-89 0.7634240028635872 1104 90-91 0.9976199301613349 1104 92-93 1.0434371476044575 1104 94-95 1.1156234018387465 1104 96-97 1.2158090472622582 1104 98-99 1.107022231026889 1104 100-101 1.670453595080776 1105 1 0.019384213958737462 1105 2 -0.031658746426259654 1105 3 -0.07795234589167421 1105 4 -0.01485738022201133 1105 5 -0.14923182511013522 1105 6 -0.055255387963775604 1105 7 -0.15703162947106364 1105 8 -0.09752412798404464 1105 9 -0.02323445840477234 1105 10-11 -0.08373356738530191 1105 12-13 -0.23445367761404157 1105 14-15 -0.008501789210953348 1105 16-17 -0.05551812862128713 1105 18-19 0.07450945202580073 1105 20-21 0.00991524863471227 1105 22-23 -0.1016432560952083 1105 24-25 -0.06334914137161007 1105 26-27 -0.10516941183164619 1105 28-29 0.0010829366743081437 1105 30-31 -0.1916687113396094 1105 32-33 -0.08441063798672843 1105 34-35 -0.14066345268970082 1105 36-37 -0.17759932468098327 1105 38-39 -0.15159359046653975 1105 40-41 -0.09438734426485951 1105 42-43 -0.5133524957687854 1105 44-45 -0.4893544552664366 1105 46-47 -0.25304524300855036 1105 48-49 -0.14578376502812063 1105 50-51 -0.10125025190576054 1105 52-53 -0.25083038872455177 1105 54-55 -0.17614221157834464 1105 56-57 0.028750755598103694 1105 58-59 -0.37585557171117756 1105 60-61 -0.3056410408267354 1105 62-63 -0.16047260502283933 1105 64-65 -0.05145686804894822 1105 66-67 -0.1564932914231889 1105 68-69 0.06263271423272698 1105 70-71 -0.011155943694220127 1105 72-73 -0.09032494114526202 1105 74-75 0.0015935627111431927 1105 76-77 0.0656286430385471 1105 78-79 0.11822354518767497 1105 80-81 -0.0357981784489283 1105 82-83 -0.09996086158534467 1105 84-85 0.2562361621634288 1105 86-87 0.6642827040985573 1105 88-89 0.8091958369318206 1105 90-91 0.800202818000983 1105 92-93 0.4369375329402345 1105 94-95 0.5244693374405216 1105 96-97 0.7196323260957804 1105 98-99 0.7884491277894199 1105 100-101 1.0552426684539107 1106 1 -0.15774262514900173 1106 2 -0.09264593199816318 1106 3 -0.05880782761924763 1106 4 -0.003330327540474798 1106 5 -0.12479838894496709 1106 6 -0.1554689617653935 1106 7 -0.11474105044875671 1106 8 -0.11807764008656108 1106 9 -0.09242940382480214 1106 10-11 -0.30091291005260956 1106 12-13 -0.579817346337343 1106 14-15 -0.46757867577953505 1106 16-17 -0.324017476034669 1106 18-19 -0.15847731826371358 1106 20-21 -0.05013755155512456 1106 22-23 -0.04077027318110282 1106 24-25 -0.04503667103464437 1106 26-27 -0.12870850770256226 1106 28-29 -0.12300195891182852 1106 30-31 -0.20096658746680163 1106 32-33 -0.3509359108865908 1106 34-35 -0.6551078641752213 1106 36-37 -0.41187347038577826 1106 38-39 -0.3335213906563794 1106 40-41 -0.38577765038731116 1106 42-43 -0.3638093064000145 1106 44-45 -0.1852965530357764 1106 46-47 -0.2169216075078353 1106 48-49 -0.30828673133092366 1106 50-51 -0.23692745883504074 1106 52-53 -0.33492952256413844 1106 54-55 -0.3799094161345806 1106 56-57 -0.5737928490530564 1106 58-59 -0.4239126433770579 1106 60-61 -0.19846407120167697 1106 62-63 -0.22789073506555013 1106 64-65 -0.3708386905453871 1106 66-67 -0.5253218984473946 1106 68-69 -0.16887238780809 1106 70-71 -0.23100199257889642 1106 72-73 -0.16721744233178626 1106 74-75 -0.23114107184034793 1106 76-77 -0.3792544846311223 1106 78-79 -0.22678565035100462 1106 80-81 -0.12705945039956745 1106 82-83 -0.18049301630769676 1106 84-85 0.014707922960770503 1106 86-87 -0.11166799594890264 1106 88-89 -0.04800782941844162 1106 90-91 0.02605711320743964 1106 92-93 0.1521086886118077 1106 94-95 -0.2767305320421585 1106 96-97 -0.21231475506226616 1106 98-99 0.05190724359388099 1106 100-101 0.5331466788952994 1107 1 -0.04931581760028081 1107 2 0.041860609769827306 1107 3 0.01225383967533844 1107 4 0.022079817337420593 1107 5 0.004848492583953146 1107 6 0.008388626342977545 1107 7 -0.010867364796126822 1107 8 -0.00927065649235459 1107 9 -0.023175285250971456 1107 10-11 -0.025096259369313145 1107 12-13 0.02912984857994161 1107 14-15 0.054156005865841905 1107 16-17 0.043744836874196835 1107 18-19 0.13916927213941932 1107 20-21 0.16834808053036454 1107 22-23 0.08632026694288442 1107 24-25 -0.014646348398748898 1107 26-27 -0.0682618008491147 1107 28-29 -0.028520077211652506 1107 30-31 0.060050597517950166 1107 32-33 0.1240103592781594 1107 34-35 0.014616765067508197 1107 36-37 -0.018637221798599057 1107 38-39 -0.17028244487428168 1107 40-41 -0.11145879913546963 1107 42-43 -0.023201538478659245 1107 44-45 -0.22422585227884895 1107 46-47 -0.21756764990945499 1107 48-49 -0.094263672455007 1107 50-51 -0.03780019404756274 1107 52-53 -0.010557797729397578 1107 54-55 0.06296335332802983 1107 56-57 -0.079565702428404 1107 58-59 -0.036556707835735835 1107 60-61 0.2058221140228298 1107 62-63 0.11339390034219576 1107 64-65 0.1545117307307713 1107 66-67 0.2904579761972599 1107 68-69 0.31119940895185394 1107 70-71 0.24111564793211926 1107 72-73 0.2828538406767471 1107 74-75 0.3323419058628403 1107 76-77 0.23811181156369443 1107 78-79 0.34507040506564834 1107 80-81 0.48599069173832277 1107 82-83 0.4723553071501918 1107 84-85 0.4232096525905291 1107 86-87 0.6661861072123827 1107 88-89 0.8530464312932757 1107 90-91 0.8543528285206534 1107 92-93 0.8879257246086567 1107 94-95 0.7095005019681864 1107 96-97 0.9412686610421268 1107 98-99 0.8345450145977509 1107 100-101 1.5489571645616316 1108 1 0.024981627342697266 1108 2 0.057520180958647416 1108 3 -0.02824304356609275 1108 4 -0.007317038503934725 1108 5 0.03865676055669098 1108 6 -0.03852544017070869 1108 7 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62-63 -1.029428707643259 2316 64-65 -0.6790936851076061 2316 66-67 -0.5216500323706903 2316 68-69 -0.714676359621027 2316 70-71 -0.4837424839156945 2316 72-73 -0.6598831455929606 2316 74-75 -0.45707008772660984 2316 76-77 -0.6371650865609126 2316 78-79 -0.638537707519685 2316 80-81 -0.4273454924417912 2316 82-83 -0.3786426818470794 2316 84-85 -0.6477783584430163 2316 86-87 -0.7127053949527209 2316 88-89 -0.4993379745748463 2316 90-91 -0.1959954370858057 2316 92-93 -0.008515339912939623 2316 94-95 0.3194863633091636 2316 96-97 0.6670961771070374 2316 98-99 0.5100666425083134 2316 100-101 0.5670759331499227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 16.0 5 36.0 6 98.0 7 221.0 8 487.0 9 957.0 10 1705.0 11 2953.0 12 4465.0 13 6300.0 14 7970.0 15 11915.0 16 17066.0 17 24158.0 18 21085.0 19 11289.0 20 8903.0 21 8828.0 22 8932.0 23 9653.0 24 10994.0 25 12506.0 26 15052.0 27 18077.0 28 21891.0 29 26836.0 30 32226.0 31 40159.0 32 50051.0 33 64741.0 34 88080.0 35 124689.0 36 175695.0 37 233705.0 38 154501.0 39 10216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45853804170055 20.027472998072486 16.471481673927816 19.04250728629915 2 15.849805497597563 22.41608593165876 39.05017925530571 22.68392931543797 3 19.3097549620331 27.702914804604788 31.29650058746167 21.690829645900447 4 12.604456881291975 18.530277717305783 37.5717000838998 31.29356531750244 5 12.3540610947813 39.41188221337106 32.776661190178196 15.457395501669446 6 28.680522771579735 38.044115476781606 17.000268251060163 16.275093500578492 7 26.021086653245963 36.66364170790313 20.155357316119606 17.1599143227313 8 27.548813131745515 32.699070416310974 19.359002269126748 20.393114182816767 9 27.972144288086966 16.332657405889943 20.461114603538793 35.2340837024843 10-11 25.065411675688544 27.96965746214929 25.393142720163592 21.571788141998578 12-13 27.407673471784054 26.100779447822497 26.89625002344296 19.595297056950493 14-15 23.743357932526298 26.058144436481168 28.014746470134032 22.1837511608585 16-17 20.237993318999433 29.763515083618504 28.177246276487754 21.821245320894313 18-19 20.313902661556035 26.411436790184457 33.46077297072965 19.813887577529858 20-21 21.965775553675716 24.677028445631237 33.85052539315308 19.506670607539977 22-23 21.451267558779307 25.009825088955782 34.032966638727 19.50594071353791 24-25 21.12966310439043 25.280868644223265 34.08309096972351 19.506377281662797 26-27 20.409828112400024 25.455239999315094 34.33536550066412 19.799566387620764 28-29 20.17142640483588 26.20286517905229 33.937274928439834 19.688433487672 30-31 20.30569243306863 25.580786677771783 33.97754434199524 20.13597654716434 32-33 20.136286270473004 25.808493940907283 33.76764475065483 20.287575037964878 34-35 20.970710082570776 25.694945546665494 33.529384906026515 19.804959464737216 36-37 20.960151264245233 25.3992986335503 33.61674995495176 20.02380014725271 38-39 20.523122181427407 25.91321548224447 33.39289485292259 20.170767483405534 40-41 20.559609217726095 26.104415705977267 33.12333922833383 20.2126358479628 42-43 20.609576851491322 26.229825680835496 32.45353561505044 20.707061852622736 44-45 20.74294128726264 26.198507415225734 32.23676539773316 20.82178589977847 46-47 21.23052224160044 26.846549794001124 31.423654851389728 20.49927311300871 48-49 21.299297247450596 27.11345552209891 30.621755405585006 20.965491824865488 50-51 21.09541828819948 27.60772236912161 30.362310155300243 20.934549187378664 52-53 21.62878130037351 27.945563787900657 29.324977598182745 21.10067731354309 54-55 20.93797366899776 27.868268345641084 29.612634054186714 21.581123931174442 56-57 21.480590935071014 27.736099662199347 29.399133932291477 21.384175470438162 58-59 21.858140033576227 27.2993967204881 29.48711049579156 21.355352750144114 60-61 21.418583357182403 27.562388755306927 30.28505548067899 20.733972406831676 62-63 21.3927366744859 27.73051449574916 30.12736625564734 20.749382574117607 64-65 21.159586550919187 27.869939818812306 29.901391236425397 21.069082393843107 66-67 21.4211109507584 27.655053597214103 29.45449638513351 21.469339066893987 68-69 21.363587813085267 27.68815691953202 29.685322753315834 21.262932514066872 70-71 21.72006004257772 27.89440040180584 29.204264621109644 21.18127493450679 72-73 21.49195938558177 27.88771921814065 29.356824228410034 21.26349716786755 74-75 20.955096077093234 28.73633366844332 28.94693928801766 21.361630966445787 76-77 21.105039450843606 27.957957149950712 29.458858522434024 21.47814487677166 78-79 21.01298612351127 28.12445218486004 29.27907323743154 21.58348845419715 80-81 21.260038011745973 28.420873683103366 28.908821546186065 21.4102667589646 82-83 21.201302936907847 28.06292092689529 29.210532752800745 21.525243383396113 84-85 20.83455466432932 28.36058990796702 28.957183770396565 21.847671657307092 86-87 20.762020541997597 28.232037982393273 28.76947775839956 22.23646371720957 88-89 20.792066291441323 28.001456220040883 28.60489880249139 22.601578686026404 90-91 20.83645068054323 27.822665312497836 28.833032369174365 22.507851637784572 92-93 21.128945395453773 27.769028464778906 29.039203128063985 22.062823011703333 94-95 20.646199681523207 27.669933785201838 29.044700084715153 22.639166448559802 96-97 20.45740474845144 27.7773972798201 29.237449478704207 22.527748493024248 98-99 20.46778571480987 28.031674927576056 28.619586957248465 22.880952400365608 100-101 20.072388692294236 27.72392321106682 29.308124095600046 22.895564001038903 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1755.0 1 1238.0 2 914.0 3 1176.0 4 998.0 5 892.5 6 1706.5 7 3547.0 8 5761.5 9 6895.5 10 5918.5 11 4499.0 12 4209.5 13 4418.0 14 4587.0 15 4656.5 16 4676.5 17 4751.5 18 4844.5 19 5005.0 20 5153.0 21 5405.0 22 5886.0 23 6568.0 24 7276.0 25 8198.0 26 9430.0 27 10903.5 28 12478.0 29 14241.5 30 16261.0 31 18091.0 32 20590.5 33 22442.0 34 23660.0 35 24945.0 36 27160.5 37 29904.5 38 31748.0 39 32638.5 40 33771.0 41 36179.5 42 37810.0 43 39531.0 44 42217.0 45 43913.0 46 43173.5 47 46028.0 48 53103.0 49 51327.5 50 43416.0 51 37943.5 52 34354.5 53 33130.5 54 32668.5 55 30764.0 56 27792.5 57 25011.5 58 23328.0 59 21533.5 60 18311.5 61 15638.5 62 13461.5 63 9972.0 64 6760.5 65 5050.0 66 3726.0 67 2922.5 68 2357.0 69 1692.0 70 1171.0 71 876.5 72 733.5 73 660.5 74 512.5 75 385.5 76 272.5 77 158.0 78 104.0 79 67.5 80 35.5 81 22.0 82 10.5 83 4.5 84 2.0 85 2.0 86 1.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04337676717520219 2 0.0 3 0.0 4 0.0 5 6.522822131609352E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0066858926848995855 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.076763832255845E-5 22-23 8.968880430962859E-4 24-25 0.0 26-27 0.0017937760861925717 28-29 0.0038729256406430526 30-31 1.2230291496767535E-4 32-33 4.076763832255845E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009825000835736586 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.630705532902338E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.446058299353507E-4 84-85 4.076763832255845E-5 86-87 0.0 88-89 0.0 90-91 0.010314212495607288 92-93 0.0014268673412895457 94-95 0.0 96-97 0.0 98-99 3.261411065804676E-4 100-101 0.013575623561411964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1226463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.5889279417867 #Duplication Level Percentage of deduplicated Percentage of total 1 69.82265508351898 30.434946811397356 2 14.964852471559961 13.046037520845916 3 5.87125528322932 7.677651704055517 4 2.916548843195982 5.085169494590841 5 1.7028205926363351 3.7112062055107864 6 1.0458373584031555 2.7352157552558216 7 0.6983574426832168 2.130845657271042 8 0.48916370689989513 1.7057697257437439 9 0.36898987772793834 1.4475485872378613 >10 1.905830225259228 14.997443083571852 >50 0.1361094078642263 4.087768522559335 >100 0.06877235178205134 5.569292649176585 >500 0.005059544499424043 1.49861096026493 >1k 0.0031856391292669904 2.5723963030262955 >5k 1.873905370157053E-4 0.7357871875547224 >10k+ 3.747810740314106E-4 2.5643098319374946 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17342 1.413984767579617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14052 1.1457337074171827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9008 0.7344697720192129 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4973 0.40547493075616636 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3331 0.27159400650488436 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2596 0.21166557817072343 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.20620271463550063 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2445 0.19935375139731082 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2096 0.170897939848165 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1451 0.1183076864120646 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1403 0.114393993133099 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1367 0.1114587231738748 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1249 0.101837560529751 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.10061453138007426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.153527664511689E-5 0.0 16-17 0.0 0.0 0.0 8.153527664511689E-5 0.0 18-19 0.0 0.0 0.0 8.153527664511689E-5 0.0 20-21 0.0 0.0 0.0 8.153527664511689E-5 0.0 22-23 0.0 0.0 0.0 4.892116598707013E-4 0.0 24-25 0.0 0.0 0.0 6.522822131609351E-4 0.0 26-27 0.0 0.0 0.0 0.0013045644263218704 0.0 28-29 0.0 0.0 0.0 0.0028537346825790915 0.0 30-31 0.0 0.0 0.0 0.005992842833416091 0.0 32-33 0.0 0.0 0.0 0.010558818325542637 0.0 34-35 0.0 0.0 0.0 0.014350208689540574 0.0 36-37 0.0 0.0 0.0 0.01622552005237826 0.0 38-39 0.0 4.0767638322558444E-5 0.0 0.018304669606828743 0.0 40-41 0.0 8.153527664511689E-5 0.0 0.02128070720437551 0.0 42-43 0.0 8.153527664511689E-5 0.0 0.02543900631327647 0.0 44-45 0.0 8.153527664511689E-5 0.0 0.031513384423337676 0.0 46-47 0.0 8.153527664511689E-5 0.0 0.03734315670346354 0.0 48-49 0.0 8.153527664511689E-5 0.0 0.04439595813326615 0.0 50-51 0.0 8.153527664511689E-5 0.0 0.05662624963003368 0.0 52-53 0.0 8.153527664511689E-5 0.0 0.08773195767014577 0.0 54-55 0.0 8.153527664511689E-5 0.0 0.12825499016276887 0.0 56-57 0.0 8.153527664511689E-5 0.0 0.17917377042764437 0.0 58-59 0.0 8.153527664511689E-5 0.0 0.2546754366010226 0.0 60-61 0.0 8.153527664511689E-5 0.0 0.37078167054366906 0.0 62-63 0.0 8.153527664511689E-5 0.0 0.4493816772295618 0.0 64-65 0.0 8.153527664511689E-5 0.0 0.5320176801093877 0.0 66-67 0.0 8.153527664511689E-5 0.0 0.6779250576658244 0.0 68-69 0.0 8.153527664511689E-5 0.0 0.8727536012093312 0.0 70-71 0.0 8.153527664511689E-5 0.0 1.0502558984657506 0.0 72-73 0.0 8.153527664511689E-5 0.0 1.2109211610949535 0.0 74-75 0.0 8.153527664511689E-5 0.0 1.4722417227425533 0.0 76-77 0.0 8.153527664511689E-5 0.0 1.6826435041252772 0.0 78-79 0.0 8.153527664511689E-5 0.0 1.8660571089384677 0.0 80-81 0.0 8.153527664511689E-5 0.0 2.081717915664802 0.0 82-83 0.0 8.153527664511689E-5 0.0 2.460571578596338 8.153527664511689E-5 84-85 0.0 8.153527664511689E-5 0.0 2.847864142660643 8.153527664511689E-5 86-87 0.0 8.153527664511689E-5 0.0 3.263490215359126 8.153527664511689E-5 88-89 0.0 8.153527664511689E-5 0.0 3.788251255847099 8.153527664511689E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 90 5.7021316E-7 36.94135 2 GGTATCA 12060 0.0 30.463013 1 GTATCAA 17355 0.0 29.630795 1 GAGTACT 12100 0.0 24.239662 12-13 TATCAAC 21275 0.0 24.177767 2 ATCAACG 21380 0.0 24.014595 3 TCAACGC 21540 0.0 23.858265 4 CAACGCA 21695 0.0 23.709702 5 GTACATG 10260 0.0 23.624546 1 AGTACTT 12110 0.0 23.415594 12-13 AACGCAG 21995 0.0 23.407907 6 GTACTTT 12540 0.0 23.293507 14-15 TAAGGTG 555 0.0 23.106173 5 GGACCGA 290 3.6379788E-12 22.929115 6 TACATGG 10500 0.0 22.843327 2 GTAAGGT 540 0.0 21.988901 4 AGAGTAC 18265 0.0 21.94814 10-11 CGTCCGC 65 2.944492E-4 21.921242 46-47 ACATGGG 10310 0.0 21.651922 3 CATGGGG 3430 0.0 21.60169 4 >>END_MODULE