FastQCFastQC Report
Thu 26 May 2016
SRR937518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191618
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145671.2224555184631316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109140.9158975443472656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69760.5854225095626283No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41460.3479302930972845No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28480.23900276766547668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.21181284606308398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23130.19410582921708133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21910.1838676488606248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.1522299931689518No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17520.14702698347960505No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15350.1288164495668914No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13760.11547324729905054No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12730.10682953765384544No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12660.10624210107601598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT250.001610109638.0290450-51
TTCGCGA250.001611441738.0226448-49
GTATCAA160500.032.187781
GGTATCA107100.028.2934681
ATCAACG192900.026.7197343
TATCAAC193100.026.7178082
TCAACGC194350.026.5192664
CAACGCA195800.026.2721025
GAGTACT95300.026.0905812-13
AACGCAG198550.025.9571116
GTACATG105450.025.2625791
GTACTTT100300.024.86046414-15
TACATGG106850.024.476062
AGTACTT97350.024.34582912-13
GTATAGA2751.8189894E-1224.2176271
ACGCAGA216550.023.6679027
CGCAGAG217650.023.4600018
CATGGGG34000.023.3536284
ACATGGG108100.023.180353
AGAGTAC157350.023.15200210-11
Produced by FastQC (version 0.11.5)