##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191618 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6442131622718 33.0 31.0 34.0 30.0 34.0 2 31.70443212506021 33.0 31.0 34.0 30.0 34.0 3 31.701567112950627 33.0 31.0 34.0 30.0 34.0 4 35.37619102766155 37.0 35.0 37.0 33.0 37.0 5 35.39786240221279 37.0 35.0 37.0 33.0 37.0 6 35.47126176341747 37.0 35.0 37.0 33.0 37.0 7 35.41795273317456 37.0 35.0 37.0 33.0 37.0 8 35.42164351327355 37.0 35.0 37.0 33.0 37.0 9 36.990320723587594 39.0 37.0 39.0 33.0 39.0 10-11 36.87139964317424 39.0 37.0 39.0 33.0 39.0 12-13 36.72631707476725 39.0 37.0 39.0 32.0 39.0 14-15 37.76170299542303 40.0 37.5 41.0 32.5 41.0 16-17 37.76260596936267 40.0 37.5 41.0 32.5 41.0 18-19 37.838856915555155 40.0 37.5 41.0 33.0 41.0 20-21 37.833935875423165 40.0 38.0 41.0 33.0 41.0 22-23 37.68966145190824 40.0 37.0 41.0 32.0 41.0 24-25 37.506882658704384 40.0 37.0 41.0 32.0 41.0 26-27 37.3271409126079 39.5 36.5 41.0 32.0 41.0 28-29 37.349586444649205 40.0 37.0 41.0 32.0 41.0 30-31 37.09478498982057 39.0 36.0 41.0 31.5 41.0 32-33 36.78650079136099 39.0 35.0 41.0 31.0 41.0 34-35 36.58198264880188 39.0 35.0 41.0 30.5 41.0 36-37 36.4983983961303 39.0 35.0 41.0 30.0 41.0 38-39 36.361163560805565 39.0 35.0 41.0 30.0 41.0 40-41 36.12126033678578 39.0 35.0 41.0 30.0 41.0 42-43 35.7654298609118 39.0 35.0 41.0 29.0 41.0 44-45 35.35882052805513 39.0 35.0 40.5 26.5 41.0 46-47 34.77723397934573 38.0 34.5 40.0 23.5 41.0 48-49 34.25970151508285 38.0 34.0 40.0 21.0 41.0 50-51 33.28024878778266 37.5 33.0 39.5 18.0 40.5 52-53 33.33345207944157 38.0 33.0 39.5 17.5 40.5 54-55 33.61054759159395 38.0 34.0 40.0 14.5 41.0 56-57 33.38673929061159 38.0 33.0 40.0 12.0 41.0 58-59 33.12911058745336 37.5 33.0 40.0 8.5 41.0 60-61 32.81977529711703 37.0 33.0 40.0 7.5 41.0 62-63 32.48893898883703 36.5 32.0 40.0 4.0 41.0 64-65 32.10671624631384 36.0 32.0 39.0 2.0 41.0 66-67 31.712215240118898 35.5 31.0 39.0 2.0 41.0 68-69 31.235806693084527 35.0 31.0 39.0 2.0 40.0 70-71 30.78086853337227 35.0 30.5 38.0 2.0 40.0 72-73 30.392251124101854 35.0 30.0 37.0 2.0 39.5 74-75 29.953629434936364 35.0 30.0 37.0 2.0 39.0 76-77 29.53575264891937 34.5 29.0 36.0 2.0 39.0 78-79 29.07530475370463 34.0 29.0 36.0 2.0 38.5 80-81 28.58923497295274 34.0 28.5 35.0 2.0 37.0 82-83 28.112630893457467 34.0 27.5 35.0 2.0 37.0 84-85 27.67905318650776 34.0 26.5 35.0 2.0 36.0 86-87 27.26667816363969 34.0 26.0 35.0 2.0 36.0 88-89 26.860675149250852 33.0 25.0 35.0 2.0 35.5 90-91 26.42745535901606 33.0 24.5 35.0 2.0 35.0 92-93 25.974023134930825 33.0 23.5 35.0 2.0 35.0 94-95 25.43890533711307 32.5 20.0 35.0 2.0 35.0 96-97 24.827870173159518 32.0 16.5 34.5 2.0 35.0 98-99 24.097041585474543 32.0 2.0 34.0 2.0 35.0 100-101 22.306445521970964 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013883434653681803 1101 2 -0.06868240534052461 1101 3 0.04381930889772079 1101 4 0.012670242179517288 1101 5 0.08402288717773843 1101 6 -0.01457993055763751 1101 7 -0.07651169795264678 1101 8 0.10923086212157074 1101 9 0.0432774100376534 1101 10-11 0.22726910826578006 1101 12-13 0.13873345553702876 1101 14-15 0.15637731376892816 1101 16-17 0.33338020389639667 1101 18-19 0.24862393779144298 1101 20-21 0.23712856988735354 1101 22-23 0.31842622729681125 1101 24-25 0.13384920539701284 1101 26-27 -0.007915186152779086 1101 28-29 0.06799826500292738 1101 30-31 0.11910335967493069 1101 32-33 0.07919876622364797 1101 34-35 0.1645261181683395 1101 36-37 0.20356396262602772 1101 38-39 0.2263592891086077 1101 40-41 0.2195967874913194 1101 42-43 0.32933471496777145 1101 44-45 0.37803913115784127 1101 46-47 0.5073074199870433 1101 48-49 0.5053904464411616 1101 50-51 0.44335390635199445 1101 52-53 0.46817776055056726 1101 54-55 0.6135973576681124 1101 56-57 0.6333051631296556 1101 58-59 0.5948155788262071 1101 60-61 0.43207111161619594 1101 62-63 0.48499395992746486 1101 64-65 0.5170043672883295 1101 66-67 0.37679126162142396 1101 68-69 0.5895800187082365 1101 70-71 0.7024146429841416 1101 72-73 0.665088432849096 1101 74-75 0.6989515217762126 1101 76-77 0.6821667448783053 1101 78-79 0.5419127591021429 1101 80-81 0.7797510193396384 1101 82-83 0.826906698922734 1101 84-85 0.8022111371892997 1101 86-87 0.9290095226776565 1101 88-89 0.8800532096598523 1101 90-91 1.0925256072810292 1101 92-93 1.1323149051322297 1101 94-95 1.1201641648172043 1101 96-97 1.2735569070008523 1101 98-99 1.2009981936112304 1101 100-101 1.4559978948273127 1102 1 0.3020183575283504 1102 2 0.19604074395850546 1102 3 0.20602907183018715 1102 4 0.0749233869508501 1102 5 0.012930641238554585 1102 6 0.014229647888718944 1102 7 0.1532736216825228 1102 8 0.09421571967629916 1102 9 0.17257959688607372 1102 10-11 0.2343191720613902 1102 12-13 0.21231387446633931 1102 14-15 0.342786562299068 1102 16-17 0.2894996592709873 1102 18-19 0.3605402244378695 1102 20-21 0.2704246461998494 1102 22-23 0.29701792838812224 1102 24-25 0.1687114361298967 1102 26-27 0.3465228830793592 1102 28-29 0.33592540823261885 1102 30-31 0.35245315860649384 1102 32-33 0.26401569939542213 1102 34-35 0.21538751339915052 1102 36-37 0.30683210235262237 1102 38-39 0.3432134012990673 1102 40-41 0.20566289515161884 1102 42-43 0.3298725591334488 1102 44-45 0.08071574529801495 1102 46-47 0.059760100618021283 1102 48-49 0.22500410500507684 1102 50-51 0.06928730595969768 1102 52-53 0.11231052133700103 1102 54-55 0.14468952945030367 1102 56-57 0.018338346322551047 1102 58-59 0.10491885067379059 1102 60-61 0.18578936367003962 1102 62-63 0.02214681767005544 1102 64-65 0.08163630349024231 1102 66-67 0.08630061837957825 1102 68-69 0.12218664100979737 1102 70-71 -0.06937360750004373 1102 72-73 0.09160585994762727 1102 74-75 0.16387052792424228 1102 76-77 0.21864930583225828 1102 78-79 0.28202773539456416 1102 80-81 0.3974072629667731 1102 82-83 0.5701813809437439 1102 84-85 0.5371750865928071 1102 86-87 0.3509564073219309 1102 88-89 0.32786440431659614 1102 90-91 0.3635197600024078 1102 92-93 0.46884756325244226 1102 94-95 0.5787709692153733 1102 96-97 0.7670586948022766 1102 98-99 1.0220788010228894 1102 100-101 1.5228147843288653 1103 1 -0.13629445653236516 1103 2 -0.14703374421006643 1103 3 -0.16603920707369113 1103 4 -0.1805400434549682 1103 5 -0.11707520757152423 1103 6 -0.30804301645215304 1103 7 -0.15401696649626473 1103 8 -0.08427954981095809 1103 9 0.049171318862136104 1103 10-11 -0.017248482606134985 1103 12-13 -0.022254086567421894 1103 14-15 -0.2498597598730825 1103 16-17 -0.27271811747883845 1103 18-19 -0.2399933129781644 1103 20-21 -0.3798649486096082 1103 22-23 -0.4818231837818914 1103 24-25 -0.32619255095025323 1103 26-27 -0.22680117450134674 1103 28-29 -0.2673753146544726 1103 30-31 -0.30116186213881946 1103 32-33 -0.166585888461654 1103 34-35 0.05170700939236639 1103 36-37 0.1321702455148639 1103 38-39 0.05444041222612839 1103 40-41 0.32572859234470997 1103 42-43 0.3847486462996912 1103 44-45 0.3499632347295929 1103 46-47 0.4411463130069251 1103 48-49 0.6382637086180196 1103 50-51 0.4359677369068109 1103 52-53 0.4818959823818716 1103 54-55 0.5510520579930258 1103 56-57 0.42651283541950846 1103 58-59 0.40873849904284043 1103 60-61 0.4649422912840464 1103 62-63 0.25198366049125553 1103 64-65 0.33659477451683983 1103 66-67 0.4654706201596497 1103 68-69 0.2835861494514198 1103 70-71 0.49292873279091154 1103 72-73 0.5044759465334252 1103 74-75 0.6677792202893436 1103 76-77 0.6642590049273274 1103 78-79 0.5830374119662629 1103 80-81 0.512576751310931 1103 82-83 0.3595782027269898 1103 84-85 0.393750678931152 1103 86-87 0.48720421603637476 1103 88-89 0.35264596195684206 1103 90-91 0.6296234635625915 1103 92-93 0.550372871566271 1103 94-95 0.5169727810144664 1103 96-97 0.5912585602271712 1103 98-99 0.5284022971339795 1103 100-101 0.5909851078136796 1104 1 -0.020041956246451775 1104 2 -0.07103205440189697 1104 3 -0.09260723935743087 1104 4 -0.005527031465263121 1104 5 -0.003886068089997252 1104 6 -0.032171329841546026 1104 7 0.07825346816254353 1104 8 0.06571193112851859 1104 9 0.1732648558126897 1104 10-11 0.12658418437329289 1104 12-13 -0.014107880816943918 1104 14-15 0.17359589783340112 1104 16-17 0.1661640417412329 1104 18-19 0.30746660659256264 1104 20-21 0.19221505587164955 1104 22-23 0.1747845729977655 1104 24-25 0.041596297716225195 1104 26-27 -0.0465361033496805 1104 28-29 0.03932603509147725 1104 30-31 0.1512119215610639 1104 32-33 0.10898646403606449 1104 34-35 0.14108362422113174 1104 36-37 0.20982439966551425 1104 38-39 0.03353778858888745 1104 40-41 0.14331041617054296 1104 42-43 0.22384051444797137 1104 44-45 0.22480889969478568 1104 46-47 0.21126354928365032 1104 48-49 0.320371464230675 1104 50-51 0.19960640270523555 1104 52-53 0.2872605398225119 1104 54-55 0.3673916160755155 1104 56-57 0.406785375336554 1104 58-59 0.1720859996905233 1104 60-61 0.34622276443033684 1104 62-63 0.17589619658055966 1104 64-65 0.25683711261384445 1104 66-67 0.36646221546459756 1104 68-69 0.42581011438556615 1104 70-71 0.4112536987730202 1104 72-73 0.3804658496962148 1104 74-75 0.394546108451582 1104 76-77 0.25249780002875255 1104 78-79 0.2877113420072739 1104 80-81 0.2570055191280183 1104 82-83 0.24906673233714471 1104 84-85 0.3287765045214748 1104 86-87 0.5533838958597386 1104 88-89 0.23996679911207863 1104 90-91 0.4621574056003048 1104 92-93 0.5122757496083317 1104 94-95 0.57538961281087 1104 96-97 0.7159490149116046 1104 98-99 0.766932490785063 1104 100-101 0.8784578876648155 1105 1 -0.008919020565684832 1105 2 0.045694956128397024 1105 3 -0.022484970736627474 1105 4 -0.03528060486306828 1105 5 -0.13821574994480557 1105 6 -0.019642555650982274 1105 7 -0.043373926794792794 1105 8 0.08142447069905501 1105 9 0.0752648789477135 1105 10-11 -0.0807008430224414 1105 12-13 -0.14486724953915342 1105 14-15 -0.05091103689112231 1105 16-17 0.0050278034196011845 1105 18-19 0.034905713272031846 1105 20-21 -0.04851936557914627 1105 22-23 -0.175205016239822 1105 24-25 -0.04344305110986113 1105 26-27 0.07796446924224654 1105 28-29 -0.01995978660686859 1105 30-31 0.08365648425121464 1105 32-33 -0.042919862237233986 1105 34-35 0.04858014432740276 1105 36-37 -0.04914331540348371 1105 38-39 0.2136835007043132 1105 40-41 0.08726022087235208 1105 42-43 0.17471154823667945 1105 44-45 0.41667847983259065 1105 46-47 0.5105081906328905 1105 48-49 0.5285946169951217 1105 50-51 0.48273152079026715 1105 52-53 0.45753260774694837 1105 54-55 0.47379192145787385 1105 56-57 0.4986817535479844 1105 58-59 0.3940584921427259 1105 60-61 0.46270570041956915 1105 62-63 0.46493308538205014 1105 64-65 0.5999301501272924 1105 66-67 0.5301473554507901 1105 68-69 0.4067291147981429 1105 70-71 0.5218489630328698 1105 72-73 0.6276530159252438 1105 74-75 0.631180424638007 1105 76-77 0.558158941279661 1105 78-79 0.5329948810495573 1105 80-81 0.8727463991073527 1105 82-83 0.9260263655698395 1105 84-85 0.9534710257613561 1105 86-87 0.7771730663533241 1105 88-89 0.870409410748227 1105 90-91 1.0744413340822234 1105 92-93 0.9536645067560947 1105 94-95 0.922090172292954 1105 96-97 0.9915853245662376 1105 98-99 1.0352417444105164 1105 100-101 1.4002784601025127 1106 1 -0.2978342903828093 1106 2 -0.3675717653611379 1106 3 -0.4032957308577316 1106 4 -0.4100567276498026 1106 5 -0.2348008624450415 1106 6 -0.25309439706038717 1106 7 -0.3886623548418413 1106 8 -0.4326347091426186 1106 9 -0.31530476361131576 1106 10-11 -0.7728889967490531 1106 12-13 -0.8779373918931199 1106 14-15 -0.7987652163102865 1106 16-17 -0.8484335598560833 1106 18-19 -0.5408215656382751 1106 20-21 -0.567418827449437 1106 22-23 -0.44403594952698455 1106 24-25 -0.3842900025668996 1106 26-27 -0.6743015082226691 1106 28-29 -0.31488475553714323 1106 30-31 -0.3932589669348019 1106 32-33 -0.33639140690241476 1106 34-35 -0.3971874685489638 1106 36-37 -0.2846745098206185 1106 38-39 -0.3325964792626692 1106 40-41 -0.33021096105506587 1106 42-43 -0.41131830755445264 1106 44-45 -0.22882182004436658 1106 46-47 -0.2628516060690842 1106 48-49 -0.08295672585595781 1106 50-51 -0.21014356757553543 1106 52-53 -0.39846585886159147 1106 54-55 -0.24984044381905335 1106 56-57 -0.32489908647847443 1106 58-59 -0.31278509162394386 1106 60-61 -0.28080572478946664 1106 62-63 -0.29329951608662697 1106 64-65 -0.2660254680829688 1106 66-67 -0.10171091301225132 1106 68-69 -0.4490512969504401 1106 70-71 -0.48620463971569094 1106 72-73 -0.31843405350399934 1106 74-75 -0.4843995744963685 1106 76-77 -0.21003534601276996 1106 78-79 -0.26480375414960733 1106 80-81 -0.23698816341235585 1106 82-83 -0.41167610290185763 1106 84-85 -0.4476711859909308 1106 86-87 -0.2679859302645582 1106 88-89 -0.16951812413951828 1106 90-91 -0.26185160963777676 1106 92-93 0.02008220341251743 1106 94-95 -0.24008246202821937 1106 96-97 -0.30102430570917704 1106 98-99 -0.26874878318744067 1106 100-101 -0.11693138575454753 1107 1 0.029228449752405794 1107 2 -0.024346096212834567 1107 3 -0.01700058733715437 1107 4 -0.006511752295132567 1107 5 -0.04490069788947437 1107 6 -0.0482930228503875 1107 7 0.06437850282329549 1107 8 -0.09901254848622187 1107 9 0.029680471991973434 1107 10-11 0.048305988296526436 1107 12-13 -0.10099379302867106 1107 14-15 0.01439580872268209 1107 16-17 -0.015085025084694337 1107 18-19 -0.014703594878234583 1107 20-21 -0.09322896908883394 1107 22-23 0.1445029262506594 1107 24-25 0.11115716673532461 1107 26-27 0.16693087236053827 1107 28-29 0.32735510035322335 1107 30-31 0.2973404637713415 1107 32-33 0.11234174325360158 1107 34-35 0.2053310297009716 1107 36-37 0.22188871106007468 1107 38-39 0.441227024440785 1107 40-41 0.3641527257497046 1107 42-43 0.33649743581487 1107 44-45 0.4015799159598572 1107 46-47 0.4880234267090131 1107 48-49 0.555418164408664 1107 50-51 0.4332097936524377 1107 52-53 0.4839434170559045 1107 54-55 0.5459377367968372 1107 56-57 0.4735526470555129 1107 58-59 0.40869035995377345 1107 60-61 0.42524216734112485 1107 62-63 0.5091767981727102 1107 64-65 0.6711906925658297 1107 66-67 0.7138500390538205 1107 68-69 0.6694971177295876 1107 70-71 0.5873380174196718 1107 72-73 0.4379001957159758 1107 74-75 0.692643973823305 1107 76-77 0.7237832932305039 1107 78-79 0.831896393684378 1107 80-81 0.9295739933676757 1107 82-83 0.7421529493264813 1107 84-85 0.8467236637618534 1107 86-87 0.8472827741548805 1107 88-89 0.6803372721809744 1107 90-91 0.8502570958457625 1107 92-93 0.9216452912806261 1107 94-95 0.8268684715300907 1107 96-97 1.0562778950790204 1107 98-99 0.9567987536095401 1107 100-101 1.1814177380725681 1108 1 -0.15107672862352928 1108 2 -0.1306982477453893 1108 3 -0.15325618662546248 1108 4 -0.1426898868206834 1108 5 -0.17724166252660467 1108 6 -0.17466633169009782 1108 7 0.0013910435076667227 1108 8 -0.08638415269128785 1108 9 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-2.687820400689528 2316 98-99 -2.5147868089660754 2316 100-101 -3.264688369379151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4405.0 3 880.0 4 817.0 5 868.0 6 986.0 7 1267.0 8 1577.0 9 1973.0 10 2581.0 11 3755.0 12 5502.0 13 7762.0 14 8843.0 15 11936.0 16 16007.0 17 21763.0 18 17718.0 19 10276.0 20 9559.0 21 9539.0 22 10301.0 23 11062.0 24 12115.0 25 13445.0 26 15308.0 27 17717.0 28 21116.0 29 24958.0 30 30247.0 31 37251.0 32 46525.0 33 60039.0 34 81033.0 35 114311.0 36 158322.0 37 211995.0 38 167377.0 39 20476.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06990176946355 19.371031466955948 15.81812316760001 19.74094359598049 2 15.057200340087379 21.627200255907333 40.22863300026096 23.086966403744327 3 18.916839575591013 27.84508482708406 30.746251067966885 22.49182452935804 4 12.08455043647353 17.643716380594814 38.11933363076949 32.152399552162166 5 12.367962044454321 39.16312264503551 32.80501314907788 15.663902161432288 6 28.614373466543412 37.4644944608415 17.838689409276988 16.0824426633381 7 26.14040428845837 36.22474038141309 20.4978419783155 17.137013351813042 8 27.242671053605434 32.33449735236134 19.86326140637721 20.559570187656014 9 28.114195944928806 16.27822878042586 20.629327403814212 34.97824787083112 10-11 24.721789597692133 27.76299867892381 25.883251521382633 21.631960202001423 12-13 27.05821053255747 25.85959141310642 27.069060428453135 20.013137625882973 14-15 23.60294729865999 25.861107583465724 27.978858237154768 22.557086880719517 16-17 20.391793727969805 29.718762011214533 27.900248361428538 21.98919589938713 18-19 20.38224086615809 26.90121750918998 32.66476994147512 20.05177168317681 20-21 22.264423216405323 25.131553995898752 32.69468414637171 19.909338641324208 22-23 21.54074312033847 25.567330826719903 32.900100158015874 19.991825894925746 24-25 21.06347885642787 25.754379798501308 33.08238843596133 20.099752909109483 26-27 20.452489106323295 25.978328688840268 33.22672301723312 20.342459187603325 28-29 20.26105477398451 26.666400341456953 32.92414567428287 20.148399210275667 30-31 20.293013025137633 26.14557704682236 32.88285787585534 20.678552052184664 32-33 20.19411032975795 26.37655671370766 32.634825473963446 20.794507482570946 34-35 20.976329258039726 26.319058940698444 32.47531923179965 20.229292569462174 36-37 20.862739162151993 26.013736385022423 32.57419518953226 20.549329263293327 38-39 20.484444341667384 26.59739025687491 32.32886042898677 20.58930497247093 40-41 20.586909670046726 26.67572856026888 32.14944304250905 20.58791872717534 42-43 20.43515718915046 26.85067104287787 31.42757071924511 21.286601048726556 44-45 20.636483132772284 26.797880043495777 31.393193567132936 21.172443256599006 46-47 21.111109709394764 27.411516305255134 30.567144245442773 20.910229739907326 48-49 21.091705076876494 27.5975254853144 29.917277865625948 21.39349157218316 50-51 20.83768453255398 28.075486489657848 29.810737028309326 21.276091949478847 52-53 21.364333324218986 28.318845030193017 28.950763606840805 21.366058038747184 54-55 20.746888315813024 28.119254171482016 29.264929271111424 21.868928241593533 56-57 21.284585570158715 28.098128403061185 28.93144877335292 21.685837253427184 58-59 21.581176727789465 27.67687809143825 29.047295720703204 21.694649460069076 60-61 21.29574665229433 27.906992387259294 29.83960609490113 20.957654865545244 62-63 21.194136280273224 28.13142495318324 29.66853653388675 21.00590223265678 64-65 20.924097432020993 28.324957264813534 29.484503922892525 21.266441380272948 66-67 21.112576444805242 28.183019384304803 29.05581548062221 21.648588690267744 68-69 21.082241782947136 28.02245499101859 29.37645530125866 21.51884792477561 70-71 21.421276814015076 28.138502657383935 29.020205031223895 21.420015497377094 72-73 21.143815718111604 28.202587312331566 29.153552824643793 21.500044144913037 74-75 20.611039751430056 28.958020185796784 28.906010540677467 21.52492952209569 76-77 20.81201605145075 28.334914259958154 29.099182963665598 21.753886724925493 78-79 20.824724583058988 28.501291207428203 28.948231453661588 21.725752755851214 80-81 21.05625189466324 28.696795438596933 28.556070049037352 21.690882617702474 82-83 20.93913265750133 28.416895828174482 28.880319885781503 21.763651628542686 84-85 20.736793473257364 28.636852621547217 28.71685204982245 21.909501855372973 86-87 20.827826151295323 28.617461886937136 28.393433084795472 22.161278876972077 88-89 20.737321764369312 28.290430380427996 28.249819331279795 22.722428523922893 90-91 20.640508350371263 28.100703085157686 28.655376438343723 22.60341212612733 92-93 21.21845524668262 27.963024933114276 28.819843241052617 21.99867657915049 94-95 20.716370575180783 27.803059889948894 28.870443807218443 22.610125727651887 96-97 20.277173812514608 27.841985871416775 29.166813804375792 22.714026511692822 98-99 20.496255402055 28.15997356309226 28.5499684043636 22.79380263048914 100-101 20.447972261737814 27.649352112486593 29.260327020928127 22.64234860484747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2645.0 1 1664.0 2 829.5 3 1010.5 4 922.5 5 930.0 6 1555.0 7 3113.0 8 5187.0 9 6211.5 10 5316.0 11 4132.0 12 4060.5 13 4236.5 14 4256.5 15 4322.5 16 4300.5 17 4302.0 18 4413.0 19 4558.0 20 4775.5 21 5136.0 22 5597.0 23 6251.5 24 7055.0 25 8074.0 26 9309.0 27 10639.5 28 12062.5 29 13974.0 30 16111.5 31 17947.0 32 20185.5 33 21900.5 34 23338.0 35 24909.0 36 26881.5 37 29340.0 38 31173.0 39 31820.0 40 32882.0 41 35165.5 42 36645.5 43 38045.0 44 40475.0 45 42396.0 46 42086.5 47 44935.0 48 51635.0 49 50022.0 50 42215.0 51 36632.5 52 33085.5 53 32460.0 54 32059.5 55 29645.0 56 26955.0 57 24552.5 58 22645.0 59 20508.0 60 17525.0 61 15352.5 62 13362.5 63 9733.0 64 6521.5 65 5011.0 66 3773.5 67 2901.0 68 2329.0 69 1723.0 70 1221.0 71 935.0 72 755.0 73 620.5 74 486.5 75 374.0 76 280.5 77 169.5 78 102.5 79 70.5 80 48.5 81 28.5 82 19.0 83 16.0 84 11.0 85 7.0 86 5.5 87 3.5 88 4.0 89 5.5 90 5.5 91 3.0 92 3.0 93 5.0 94 4.5 95 3.0 96 2.0 97 5.5 98 9.5 99 7.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36160917340959936 2 0.30949515700501334 3 0.30320119367112613 4 0.30949515700501334 5 0.3106700301606723 6 0.22817715073119071 7 0.2153374655300608 8 0.22104399228611854 9 0.2130716387298614 10-11 0.23619146404300706 12-13 0.22377137639746966 14-15 0.2179389703747342 16-17 0.22028871668605207 18-19 0.2140786728632834 20-21 0.20707139368488894 22-23 0.2095470192628846 24-25 0.19968647670646128 26-27 0.20107114863991649 28-29 0.2178130911080564 30-31 0.2318696092204045 32-33 0.22280630201960694 34-35 0.23392563724280768 36-37 0.20480556688468954 38-39 0.2031271766623196 40-41 0.20048371206208704 42-43 0.20656787661817796 44-45 0.21290379970762444 46-47 0.21814876915253043 48-49 0.2264148409977023 50-51 0.23241508604267472 52-53 0.25305928577782477 54-55 0.22758971415336124 56-57 0.19632969626172145 58-59 0.19683321332843243 60-61 0.1926791975280669 62-63 0.20199426326221992 64-65 0.1944415072615553 66-67 0.19129452559461171 68-69 0.18600759639414646 70-71 0.2001060742620538 72-73 0.19746260966182114 74-75 0.20270757910672718 76-77 0.20493144615136732 78-79 0.20316913641787887 80-81 0.20329501568455666 82-83 0.19456738652823302 84-85 0.18751814759427937 86-87 0.189825934150038 88-89 0.19188196217244116 90-91 0.19800808648409138 92-93 0.19100080730569696 94-95 0.18978397439447875 96-97 0.18932241708332706 98-99 0.19901512061751334 100-101 0.1857977976163502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.072920550819354 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8946405890463 33.123960638888434 2 13.954826150655272 12.858791930792734 3 5.474410250944975 7.566662056631365 4 2.805066802720896 5.169504797660028 5 1.5103364254721958 3.4792805067894483 6 0.9912848741773977 2.7402833550722523 7 0.6643876778833854 2.1427196488645204 8 0.4574188052457636 1.6859696218031024 9 0.32957848099161136 1.3666178852987614 >10 1.7231543260218745 14.402426719312642 >50 0.12479282614612991 3.9042297345740264 >100 0.06227313163027978 5.220931234688612 >500 0.004734212457283214 1.4030923775356516 >1k 0.0025491913231524997 2.212649414499421 >5k 1.8208509451089283E-4 0.5852300403999225 >10k+ 3.6417018902178567E-4 2.137650037188995 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14567 1.2224555184631316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10914 0.9158975443472656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6976 0.5854225095626283 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4146 0.3479302930972845 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2848 0.23900276766547668 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2524 0.21181284606308398 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2313 0.19410582921708133 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2191 0.1838676488606248 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1814 0.1522299931689518 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1752 0.14702698347960505 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1535 0.1288164495668914 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1376 0.11547324729905054 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1273 0.10682953765384544 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1266 0.10624210107601598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.1959755559248016E-5 0.0 12-13 0.0 0.0 0.0 8.391951111849603E-5 0.0 14-15 0.0 0.0 0.0 8.391951111849603E-5 0.0 16-17 0.0 0.0 0.0 1.2587926667774403E-4 0.0 18-19 0.0 0.0 0.0 1.6783902223699206E-4 0.0 20-21 0.0 0.0 0.0 1.6783902223699206E-4 0.0 22-23 0.0 0.0 0.0 2.5175853335548807E-4 0.0 24-25 0.0 0.0 0.0 2.5175853335548807E-4 0.0 26-27 0.0 0.0 0.0 7.972353556257122E-4 0.0 28-29 0.0 0.0 0.0 0.002223867044640145 0.0 30-31 0.0 0.0 0.0 0.005664567000498482 0.0 32-33 0.0 0.0 0.0 0.008769588911882835 0.0 34-35 0.0 0.0 0.0 0.01183265106770794 0.0 36-37 0.0 0.0 0.0 0.013678880312314852 0.0 38-39 0.0 0.0 0.0 0.015818827845836502 0.0 40-41 0.0 0.0 0.0 0.01804269489047665 0.0 42-43 0.0 0.0 0.0 0.021399475335216488 0.0 44-45 0.0 0.0 0.0 0.025469571624463547 0.0 46-47 0.0 0.0 0.0 0.03151177642499525 4.1959755559248016E-5 48-49 0.0 0.0 0.0 0.03919041169233764 8.391951111849603E-5 50-51 0.0 0.0 0.0 0.05056150544889386 8.391951111849603E-5 52-53 0.0 0.0 0.0 0.08085644896267093 8.391951111849603E-5 54-55 0.0 0.0 0.0 0.1161446033879985 8.391951111849603E-5 56-57 0.0 0.0 0.0 0.15701340530270608 8.391951111849603E-5 58-59 0.0 0.0 0.0 0.2206663544860853 8.391951111849603E-5 60-61 0.0 0.0 0.0 0.32166348611719525 8.391951111849603E-5 62-63 0.0 0.0 0.0 0.3924915535012059 8.391951111849603E-5 64-65 0.0 0.0 0.0 0.46126359286281343 8.391951111849603E-5 66-67 0.0 0.0 0.0 0.596374005763592 8.391951111849603E-5 68-69 0.0 0.0 0.0 0.7773044717350694 8.391951111849603E-5 70-71 0.0 0.0 0.0 0.9333108429043536 8.391951111849603E-5 72-73 0.0 0.0 0.0 1.0755963740057637 8.391951111849603E-5 74-75 0.0 0.0 0.0 1.322319736694142 8.391951111849603E-5 76-77 0.0 0.0 0.0 1.5071944196881888 8.391951111849603E-5 78-79 0.0 0.0 0.0 1.6721382187915927 8.391951111849603E-5 80-81 0.0 0.0 0.0 1.8813915197655624 8.391951111849603E-5 82-83 0.0 0.0 0.0 2.2580642454209316 8.391951111849603E-5 84-85 0.0 0.0 0.0 2.629911599186988 8.391951111849603E-5 86-87 0.0 0.0 0.0 3.0320538964668207 8.391951111849603E-5 88-89 0.0 0.0 0.0 3.548116930090012 8.391951111849603E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGAT 25 0.0016101096 38.02904 50-51 TTCGCGA 25 0.0016114417 38.02264 48-49 GTATCAA 16050 0.0 32.18778 1 GGTATCA 10710 0.0 28.293468 1 ATCAACG 19290 0.0 26.719734 3 TATCAAC 19310 0.0 26.717808 2 TCAACGC 19435 0.0 26.519266 4 CAACGCA 19580 0.0 26.272102 5 GAGTACT 9530 0.0 26.09058 12-13 AACGCAG 19855 0.0 25.957111 6 GTACATG 10545 0.0 25.262579 1 GTACTTT 10030 0.0 24.860464 14-15 TACATGG 10685 0.0 24.47606 2 AGTACTT 9735 0.0 24.345829 12-13 GTATAGA 275 1.8189894E-12 24.217627 1 ACGCAGA 21655 0.0 23.667902 7 CGCAGAG 21765 0.0 23.460001 8 CATGGGG 3400 0.0 23.353628 4 ACATGGG 10810 0.0 23.18035 3 AGAGTAC 15735 0.0 23.152002 10-11 >>END_MODULE