FastQCFastQC Report
Thu 26 May 2016
SRR937518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191618
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174571.4649829055955852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144951.2164133136625999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.7659333779785132No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48750.40910761670266815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.28457106220282No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25780.21634449966348276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.20937918024064756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24390.2046796876180118No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21030.17648273188219715No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13710.11505364974345805No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13550.11371093756556212No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13060.10959888152075581No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.10825616934285988No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12700.10657777912048995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118200.030.5206011
GTATCAA167350.028.827641
GTGTAAG3950.026.4724641
GGACCGA2351.7644197E-1024.2543986
GAGTACT117250.023.57791912-13
TATCAAC205900.023.4617252
ATCAACG206000.023.4042193
TCAACGC207350.023.251844
CAACGCA209950.022.9638925
AACGCAG212900.022.6447496
GTACTTT121950.022.57089214-15
GTACATG103200.022.5215321
AGTACTT118100.022.22172412-13
TACATGG104950.021.7699782
CATGGGG32450.021.5178054
AGAGTAC180950.020.98723410-11
ATGGGGA13200.020.8712945
CGTAACA1150.00372327120.6522562
ACTTTTT135400.020.52174616-17
ACGCAGA235300.020.3889457
Produced by FastQC (version 0.11.5)