##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937518_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191618 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.074554932872786 31.0 31.0 34.0 31.0 34.0 2 32.28123945761141 33.0 31.0 34.0 31.0 34.0 3 32.314444729770784 34.0 31.0 34.0 30.0 34.0 4 35.994530965460406 37.0 35.0 37.0 35.0 37.0 5 35.858842347128025 37.0 35.0 37.0 35.0 37.0 6 35.96980156392401 37.0 35.0 37.0 35.0 37.0 7 35.926007327851714 37.0 35.0 37.0 35.0 37.0 8 35.913760114399075 37.0 35.0 37.0 35.0 37.0 9 37.566617825511194 39.0 37.0 39.0 35.0 39.0 10-11 37.49298223088272 39.0 37.0 39.0 35.0 39.0 12-13 37.387913744169694 39.0 37.0 39.0 34.0 39.0 14-15 38.4465273267104 40.0 38.0 41.0 34.0 41.0 16-17 38.458583623275246 40.0 38.0 41.0 34.0 41.0 18-19 38.59048117769285 40.0 38.0 41.0 34.0 41.0 20-21 38.55352050741093 40.0 38.0 41.0 34.0 41.0 22-23 38.42922815868844 40.0 38.0 41.0 34.0 41.0 24-25 38.26331508923162 40.0 38.0 41.0 34.0 41.0 26-27 38.0131715868676 40.0 37.0 41.0 33.0 41.0 28-29 37.95321739013677 40.0 37.0 41.0 33.0 41.0 30-31 37.90091749201506 40.0 37.0 41.0 33.0 41.0 32-33 37.69974522036424 40.0 36.0 41.0 33.0 41.0 34-35 37.50520846445757 40.0 35.5 41.0 33.0 41.0 36-37 37.273970349558326 40.0 35.0 41.0 33.0 41.0 38-39 37.07018650272151 39.5 35.0 41.0 32.5 41.0 40-41 36.83716803539389 39.0 35.0 41.0 31.5 41.0 42-43 36.500718770612735 39.0 35.0 41.0 31.0 41.0 44-45 36.00650963647746 39.0 35.0 41.0 30.0 41.0 46-47 35.38957409169717 39.0 35.0 41.0 27.0 41.0 48-49 34.82191398585788 39.0 35.0 40.5 23.5 41.0 50-51 34.497552907055784 39.0 35.0 40.0 22.5 41.0 52-53 34.23072788427164 38.0 34.0 40.0 21.0 41.0 54-55 33.999947969903104 38.0 34.0 40.0 20.0 41.0 56-57 33.72754985238558 38.0 34.0 40.0 18.5 41.0 58-59 33.45292618943319 37.5 33.5 40.0 16.0 41.0 60-61 33.09089238329733 37.0 33.0 40.0 12.5 41.0 62-63 32.726708978884176 36.5 33.0 40.0 8.0 41.0 64-65 32.377627729691895 36.0 32.0 39.0 7.0 41.0 66-67 31.967216423384002 35.5 32.0 39.0 2.0 40.0 68-69 31.515476436240473 35.0 31.0 38.5 2.0 40.0 70-71 31.117367310664996 35.0 31.0 38.0 2.0 40.0 72-73 30.68239737902583 35.0 31.0 37.0 2.0 39.5 74-75 30.254363814578163 35.0 30.0 37.0 2.0 39.0 76-77 28.970053742054922 33.5 28.5 35.0 2.0 37.5 78-79 29.563647494415157 34.0 30.0 36.0 2.0 38.0 80-81 29.375606108669054 34.0 30.0 35.0 2.0 37.0 82-83 29.032426918693744 34.0 29.5 35.0 2.0 37.0 84-85 28.700153488785837 34.0 29.0 35.0 2.0 36.0 86-87 28.382550448214108 34.0 29.0 35.0 2.0 36.0 88-89 28.00666572676814 34.0 29.0 35.0 2.0 35.5 90-91 27.554851051259718 34.0 27.0 35.0 2.0 35.0 92-93 27.14077749748661 33.0 26.5 35.0 2.0 35.0 94-95 26.728220788876975 33.0 25.5 35.0 2.0 35.0 96-97 26.308652605113384 33.0 25.0 35.0 2.0 35.0 98-99 25.706697951860413 33.0 23.5 35.0 2.0 35.0 100-101 24.2439452072728 31.5 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0686979053170731 1101 2 0.1206024652122224 1101 3 0.05543887823282745 1101 4 0.09197794298736284 1101 5 -0.12286084412243525 1101 6 -0.012148977827528995 1101 7 0.061995606789921 1101 8 -0.06595459052091712 1101 9 0.06835785212028611 1101 10-11 0.028372377112781066 1101 12-13 0.11900307304080116 1101 14-15 0.2338869688810732 1101 16-17 0.10139520154936577 1101 18-19 0.09524531595243246 1101 20-21 -0.03802165095446952 1101 22-23 -0.11149461193313925 1101 24-25 0.0745494331283254 1101 26-27 -0.002562827637660803 1101 28-29 -0.05704256918085093 1101 30-31 0.25203902311083937 1101 32-33 0.19813970403920678 1101 34-35 0.060401428500476584 1101 36-37 0.23437637439285908 1101 38-39 0.3006240614021465 1101 40-41 0.44494235782699576 1101 42-43 0.24323808608653508 1101 44-45 0.2609616096502947 1101 46-47 0.4965843160397796 1101 48-49 0.4130704713360558 1101 50-51 0.43276463415645594 1101 52-53 0.5467199869420512 1101 54-55 0.4882563288834376 1101 56-57 0.43266060511662374 1101 58-59 0.5507113246573496 1101 60-61 0.415300090630879 1101 62-63 0.36111964584649314 1101 64-65 0.4468641843624752 1101 66-67 0.4861039044206983 1101 68-69 0.34302583489552063 1101 70-71 0.42945342232332706 1101 72-73 0.4112893529826209 1101 74-75 0.5006385713588308 1101 76-77 0.31758273563453443 1101 78-79 0.4222626228917754 1101 80-81 0.34188743264364874 1101 82-83 0.4880000431204259 1101 84-85 0.15454707869883322 1101 86-87 0.09394960887619419 1101 88-89 0.4528329271007472 1101 90-91 0.6227999358061034 1101 92-93 0.6175878030488242 1101 94-95 0.4622163552140286 1101 96-97 0.6129288925177647 1101 98-99 0.5127500688882911 1101 100-101 0.6847380143491115 1102 1 0.14387578939569323 1102 2 0.025571107105236024 1102 3 0.04310121509259801 1102 4 0.033007892935515315 1102 5 -0.08468441393279136 1102 6 -0.024393120764301557 1102 7 0.030176490518762478 1102 8 0.055858976335521504 1102 9 -0.0014716650806292364 1102 10-11 0.0012803569404340465 1102 12-13 -0.05187955580799297 1102 14-15 0.13989298466085387 1102 16-17 0.13425725348150763 1102 18-19 0.015097883617592345 1102 20-21 -0.03432986882605604 1102 22-23 -0.10264315126703849 1102 24-25 -0.03922283817254879 1102 26-27 0.18178815244995405 1102 28-29 0.17490628062364522 1102 30-31 0.06264680967714042 1102 32-33 0.12996677158756853 1102 34-35 0.21649179785584494 1102 36-37 0.15964837605067572 1102 38-39 0.10396899481123256 1102 40-41 0.16458341327870585 1102 42-43 0.07972913389595959 1102 44-45 0.2859405676045199 1102 46-47 0.26645499036988696 1102 48-49 0.5933042965636872 1102 50-51 0.47514576565204436 1102 52-53 0.4351322569575373 1102 54-55 0.4547114146727793 1102 56-57 0.4805827056710257 1102 58-59 0.42177230106594976 1102 60-61 0.26903799428693986 1102 62-63 0.48512722963462096 1102 64-65 0.5696749567363639 1102 66-67 0.44625482213404055 1102 68-69 0.45242799360548247 1102 70-71 0.5298856751755885 1102 72-73 0.45630944334703116 1102 74-75 0.362900614968904 1102 76-77 0.19924218778741576 1102 78-79 0.5148657515751651 1102 80-81 0.41614885992674644 1102 82-83 0.4633854843730525 1102 84-85 0.279558047753536 1102 86-87 0.3748993374410077 1102 88-89 0.43566064022690654 1102 90-91 0.4839259355350194 1102 92-93 0.3011457153318169 1102 94-95 0.183488452451968 1102 96-97 0.5242080882275353 1102 98-99 0.5382032195921838 1102 100-101 0.39378677380566884 1103 1 -0.06976158518688891 1103 2 -0.03581233628179348 1103 3 -0.10538267565372905 1103 4 -0.13793962640612278 1103 5 -0.1617139288286893 1103 6 -0.09472576830548007 1103 7 -0.11052311435354767 1103 8 -0.18289114627151548 1103 9 -0.2284842067216175 1103 10-11 -0.19446486582496192 1103 12-13 -0.12671828970409393 1103 14-15 -0.10440926925016214 1103 16-17 -0.19701527240926708 1103 18-19 -0.26591544190117844 1103 20-21 -0.22580291727511081 1103 22-23 -0.2448895221994647 1103 24-25 -0.24093291883285417 1103 26-27 -0.11465448743409468 1103 28-29 -0.16900254090772648 1103 30-31 -0.17493698189370832 1103 32-33 -0.0903719104401901 1103 34-35 -0.013040293301635586 1103 36-37 0.0944803610092606 1103 38-39 0.09041703142340651 1103 40-41 0.0037195412049797483 1103 42-43 -0.029760469632215347 1103 44-45 -0.0707010206239147 1103 46-47 -0.17734234452635889 1103 48-49 -0.1137885864960424 1103 50-51 -0.11164609541994963 1103 52-53 0.10753047494740997 1103 54-55 0.17962338987931048 1103 56-57 0.18977655880978972 1103 58-59 0.059934797882199575 1103 60-61 0.07015403120750108 1103 62-63 0.1575648029717982 1103 64-65 0.22566885685935034 1103 66-67 0.004791360243938669 1103 68-69 -0.03940223993923908 1103 70-71 0.10722680369320159 1103 72-73 0.3493555351188604 1103 74-75 0.21566660968842655 1103 76-77 0.0015707730494618488 1103 78-79 0.22792329070749773 1103 80-81 0.195277165220908 1103 82-83 0.2589863234694221 1103 84-85 0.18882742279465603 1103 86-87 0.20456677062875173 1103 88-89 0.1406856520637838 1103 90-91 0.29516981880584936 1103 92-93 0.2702101923073208 1103 94-95 0.3127844922545542 1103 96-97 0.2512444448991964 1103 98-99 0.1058810966799868 1103 100-101 0.1511987984586689 1104 1 -0.08960166024798255 1104 2 0.024921563277317205 1104 3 0.04246737020915958 1104 4 0.008327974789807513 1104 5 -0.17413425227291413 1104 6 -0.046909331886851646 1104 7 -0.031733474464736844 1104 8 -0.06021784173961464 1104 9 -0.14804093549573594 1104 10-11 0.03609767224823912 1104 12-13 -0.03546689806304215 1104 14-15 0.05641600598551122 1104 16-17 0.04401520715548912 1104 18-19 -0.012517737146147567 1104 20-21 -0.13668070726743764 1104 22-23 -0.07995817649131709 1104 24-25 -0.0827263794619526 1104 26-27 -0.15817288276984698 1104 28-29 -0.18533229623564296 1104 30-31 -0.06268610606426961 1104 32-33 -0.06797611722230101 1104 34-35 0.0020512807640074016 1104 36-37 0.03885206279080222 1104 38-39 -0.10157983636310775 1104 40-41 -0.17321842836447132 1104 42-43 -0.056827742202479214 1104 44-45 -0.059151275145431725 1104 46-47 0.0408588033295203 1104 48-49 -0.17834906132280537 1104 50-51 -0.09779211911927632 1104 52-53 -0.013552799452696718 1104 54-55 0.029239798756876212 1104 56-57 -0.1463768169554669 1104 58-59 -0.07215848699751604 1104 60-61 -0.021297500006099312 1104 62-63 -0.04433576442097831 1104 64-65 0.011840437740254117 1104 66-67 -0.026235524977472835 1104 68-69 0.010285472307902666 1104 70-71 -0.08730360935286186 1104 72-73 -0.047075445319975984 1104 74-75 -0.19747281582347398 1104 76-77 -0.14134346918594076 1104 78-79 -0.20472244941214157 1104 80-81 -0.13174214387161598 1104 82-83 -0.17359173096387437 1104 84-85 -0.06812961546642882 1104 86-87 -0.15435525312659237 1104 88-89 -0.11621828794335798 1104 90-91 0.04944685398606552 1104 92-93 -0.0878955187308108 1104 94-95 -0.20071004971174133 1104 96-97 -0.08426474985119725 1104 98-99 -0.03986194044678015 1104 100-101 0.185151922619891 1105 1 -0.039523193946074286 1105 2 -0.07542854680685451 1105 3 -0.07759224104525941 1105 4 -0.046325057633929134 1105 5 -0.18723446164491264 1105 6 -0.018996149933713014 1105 7 -0.051887553748478865 1105 8 -5.221444691585475E-4 1105 9 -0.13803195139335145 1105 10-11 -0.10934434173302776 1105 12-13 -0.1967949643217608 1105 14-15 -0.19426609174455933 1105 16-17 -0.1381463370304843 1105 18-19 -0.10563198176821231 1105 20-21 -0.12864822568869982 1105 22-23 -0.23009332361080936 1105 24-25 -0.291766584284062 1105 26-27 -0.2654542471195427 1105 28-29 -0.22787722638713603 1105 30-31 -0.3284127194432571 1105 32-33 -0.18395856544803735 1105 34-35 -0.20750849821935446 1105 36-37 -0.1642144851160623 1105 38-39 -0.11161176270118034 1105 40-41 -0.0712482879734111 1105 42-43 -0.2141305064526975 1105 44-45 -0.06716332078313059 1105 46-47 -0.06569997699182295 1105 48-49 -0.36959708038605754 1105 50-51 -0.2150961557960045 1105 52-53 -0.12779992781739935 1105 54-55 0.0090510169317497 1105 56-57 -0.020689417448245706 1105 58-59 -0.05385790900740517 1105 60-61 0.036203454313785244 1105 62-63 -0.0708303835991444 1105 64-65 0.004629635224702611 1105 66-67 -0.13205706908633275 1105 68-69 -0.12322542236217515 1105 70-71 0.07035755503307328 1105 72-73 0.054310535624473744 1105 74-75 0.03998279384234138 1105 76-77 0.039657853366470874 1105 78-79 0.11498358150396726 1105 80-81 0.25539882941899705 1105 82-83 0.22964871523369368 1105 84-85 0.025394890311545737 1105 86-87 0.02405822379814282 1105 88-89 0.18223687058941707 1105 90-91 0.17148277497223674 1105 92-93 0.31505284774344844 1105 94-95 0.36112737273771955 1105 96-97 0.40219489687693155 1105 98-99 0.3529351615054317 1105 100-101 0.32101989101744266 1106 1 -0.34325712579672185 1106 2 -0.17081415147627865 1106 3 -0.18266005857991985 1106 4 -0.16365176275904503 1106 5 -0.28098156334957025 1106 6 -0.17208673750705827 1106 7 -0.212966203951801 1106 8 -0.09980264587167653 1106 9 -0.23093939761645288 1106 10-11 -0.24433967354533337 1106 12-13 -0.3932094251924525 1106 14-15 -0.18385428133274928 1106 16-17 -0.11552551547067225 1106 18-19 -0.18495963907995616 1106 20-21 -0.5736334791620195 1106 22-23 -0.36656681180843975 1106 24-25 -0.29790756973539345 1106 26-27 -0.16417374159500753 1106 28-29 -0.35918252586219523 1106 30-31 -0.1276171695760837 1106 32-33 -0.22255736717681174 1106 34-35 -0.2466926267934042 1106 36-37 -0.306942726518038 1106 38-39 -0.28493961374785925 1106 40-41 -0.25722620631207604 1106 42-43 -0.37163083106177197 1106 44-45 0.032494345452782625 1106 46-47 -0.09760977070533272 1106 48-49 -0.1978557948516766 1106 50-51 0.0782276144508387 1106 52-53 0.11279578651836886 1106 54-55 0.08442174110884082 1106 56-57 -0.038945431990725865 1106 58-59 -0.0796368162186738 1106 60-61 -0.03869962669008942 1106 62-63 -0.016079446354837046 1106 64-65 -0.2666081455621523 1106 66-67 -0.024400210766341246 1106 68-69 0.02356754299843189 1106 70-71 0.01862847067799578 1106 72-73 0.05522603627471767 1106 74-75 -0.011583264620270484 1106 76-77 -0.13123347163626065 1106 78-79 -0.08515409396527573 1106 80-81 0.0978486283674691 1106 82-83 -0.06295370150665747 1106 84-85 -0.04276394760856306 1106 86-87 -0.013068810676372777 1106 88-89 -0.009236937064759587 1106 90-91 0.20842473074708323 1106 92-93 0.327764333133981 1106 94-95 -0.12557437231946267 1106 96-97 -0.06222217735446378 1106 98-99 -0.19415994792389668 1106 100-101 -0.03734626390043516 1107 1 -0.03982882147913358 1107 2 0.04219979964349818 1107 3 0.05684239835250793 1107 4 0.04083798197081023 1107 5 -0.10285871292792592 1107 6 -0.03889173726003747 1107 7 0.013223593603051143 1107 8 -0.09018013558220161 1107 9 -0.15905179704943606 1107 10-11 0.05971378991230836 1107 12-13 -0.048062674309818476 1107 14-15 -0.016891186623986698 1107 16-17 -0.10698420746292925 1107 18-19 -0.03697934014368087 1107 20-21 -0.09813439694884352 1107 22-23 -0.15338564487004902 1107 24-25 -0.053626160714102866 1107 26-27 -0.08544929426228975 1107 28-29 -0.10304589533694752 1107 30-31 0.005081883754357364 1107 32-33 0.027918627852322686 1107 34-35 -0.014038417705229733 1107 36-37 -0.07586376644842119 1107 38-39 -0.0603003628747274 1107 40-41 0.026738556754516196 1107 42-43 0.14936995561264865 1107 44-45 0.11342490148338413 1107 46-47 0.31386465477532255 1107 48-49 0.3425078235466259 1107 50-51 0.4135428352707322 1107 52-53 0.33110363844116364 1107 54-55 0.35025878929359777 1107 56-57 0.42776341866654377 1107 58-59 0.46313623011150895 1107 60-61 0.34084987869312044 1107 62-63 0.4034574418347816 1107 64-65 0.5044733987519336 1107 66-67 0.5411046859968884 1107 68-69 0.44673187434417727 1107 70-71 0.4508984634193105 1107 72-73 0.5426449461035894 1107 74-75 0.6976455284235534 1107 76-77 0.5847447599044422 1107 78-79 0.3785486723164162 1107 80-81 0.36247336186725576 1107 82-83 0.3825776457587935 1107 84-85 0.28568022320999376 1107 86-87 0.3211310046261566 1107 88-89 0.30117832821933277 1107 90-91 0.32318798735115806 1107 92-93 0.34619987713644207 1107 94-95 0.1571020688718754 1107 96-97 0.29375369796334283 1107 98-99 0.3830519563222481 1107 100-101 0.5799651337577849 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46-47 -0.3956561141452397 2316 48-49 -0.4311344670838366 2316 50-51 -0.5582779739778232 2316 52-53 -0.47672036717488453 2316 54-55 -0.5851155889830508 2316 56-57 -0.2898341526916113 2316 58-59 -0.19707982342017516 2316 60-61 -0.28435922946059833 2316 62-63 -0.3843519262105062 2316 64-65 -0.3055742480345174 2316 66-67 -0.39512479526552724 2316 68-69 -0.4232831366340015 2316 70-71 -0.17006126832480462 2316 72-73 -0.09194228933725412 2316 74-75 -0.30513822387290546 2316 76-77 -0.1302912365705886 2316 78-79 -0.31812314737261005 2316 80-81 -0.30370787515683517 2316 82-83 -0.2255704774955234 2316 84-85 0.04164540670240058 2316 86-87 -0.23205341382912792 2316 88-89 -0.405482139884203 2316 90-91 -0.7599119663463192 2316 92-93 -0.38103533966390657 2316 94-95 -0.1410973605615915 2316 96-97 -0.24636892903252416 2316 98-99 0.05416963940390218 2316 100-101 0.11453705949451276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 13.0 5 40.0 6 86.0 7 198.0 8 444.0 9 913.0 10 1618.0 11 2828.0 12 4344.0 13 5839.0 14 7795.0 15 11374.0 16 16025.0 17 23494.0 18 20420.0 19 10472.0 20 8033.0 21 7879.0 22 8063.0 23 8432.0 24 9396.0 25 10564.0 26 12544.0 27 15266.0 28 18371.0 29 22701.0 30 27381.0 31 34293.0 32 43090.0 33 55842.0 34 77030.0 35 110562.0 36 160716.0 37 238268.0 38 198295.0 39 18985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48432398305078 19.977298047917575 16.540857216979113 18.997520752052537 2 15.836031345615792 22.41657980997266 39.09625400086269 22.651134843548856 3 19.209595692579335 27.69285123252586 31.424416213920903 21.6731368609739 4 12.546218670748512 18.616368668482686 37.55205107677125 31.285361583997556 5 12.356597019685518 39.32370109569151 32.80561652056048 15.514085364062494 6 28.61286083291793 38.19344790024991 16.92480308286716 16.268888183964997 7 25.90519780667966 36.77730615012529 20.17517358750875 17.1423224556863 8 27.491276566819234 32.79339519879693 19.296368467075858 20.418959767307978 9 27.933280631880354 16.2943157958339 20.48139588358014 35.29100768870561 10-11 25.060673806538674 27.979772041039997 25.35166471134206 21.607889441079273 12-13 27.48671359483021 26.171186387335688 26.816906727652295 19.525193290181804 14-15 23.741920649067065 26.044713993914158 28.15914999605578 22.054215360962992 16-17 20.176600219197763 29.738011678239168 28.29153302484521 21.793855077717858 18-19 20.211175057778583 26.473962293285265 33.573007457087755 19.741855191848394 20-21 21.935721011263677 24.62114536705555 33.99487083948044 19.448262782200338 22-23 21.369542276052627 24.953623376923094 34.203100446667406 19.47373390035687 24-25 21.048031453111953 25.176336316679464 34.307683270183745 19.467948960024838 26-27 20.322729267774825 25.39554392587365 34.52928741250114 19.752439393850384 28-29 20.09696345114021 26.12851979257844 34.18112712249168 19.593389633789673 30-31 20.207015603377304 25.537407236146997 34.17423281786437 20.081344342611327 32-33 20.075317792832013 25.75411153327305 33.94537257131588 20.225198102579057 34-35 20.855467104390836 25.58533859005151 33.75981228883753 19.799382016720124 36-37 20.838543353804166 25.29812506398884 33.88211283183984 19.981218750367148 38-39 20.4239385068036 25.897450637225305 33.59152710426175 20.087083751709343 40-41 20.43885726753761 26.065830688119302 33.34899831531511 20.14631372902798 42-43 20.49815543836193 26.17342949980927 32.65304238316273 20.675372678666072 44-45 20.688047679709438 26.145417407256353 32.443660636210595 20.722874276823614 46-47 21.185984098931034 26.860747320030416 31.61155672371515 20.341711857323403 48-49 21.243888561602795 27.14431134809981 30.72146442903682 20.890335661260572 50-51 21.040761384940478 27.576119192560032 30.520980716974734 20.862138705524757 52-53 21.53490075859265 27.913683494116597 29.466786448134023 21.08462929915673 54-55 20.891342695393995 27.81063226638067 29.78819554588803 21.50982949233731 56-57 21.512682755715336 27.711900961549755 29.493596102106544 21.281820180628355 58-59 21.80283446540754 27.253574551576094 29.59224348742634 21.35134749559003 60-61 21.335948265299788 27.510452175109805 30.45665641170241 20.696943147887996 62-63 21.319751799653915 27.68743842405872 30.268550827530298 20.72425894875707 64-65 21.118344972969524 27.909489450478258 30.027827709886896 20.944337866665325 66-67 21.369599989258305 27.691970077659118 29.584061335092287 21.354368597990295 68-69 21.331500531210505 27.673214066924135 29.78785986784355 21.20742553402181 70-71 21.652828339283225 27.866522660785588 29.439006460123966 21.04164253980722 72-73 21.472737068422934 27.88355832154264 29.519862909086637 21.123841700947786 74-75 20.906825845195357 28.68780095634675 29.161274838077304 21.24409836038059 76-77 21.09023193674483 27.93680525134733 29.601642472671614 21.371320339236235 78-79 21.074832706454586 28.166241194745297 29.345310325960167 21.413615772839954 80-81 21.24808453715872 28.413803752544858 29.026584022732116 21.3115276875643 82-83 21.089205211092203 28.092575435628376 29.361738552481846 21.456480800797575 84-85 20.770314946125705 28.312212863786296 29.160304510721364 21.757167679366642 86-87 20.77318402373915 28.29392473091209 28.788546329444504 22.144344915904256 88-89 20.746665458225706 28.025172496555108 28.727369005839122 22.50079303938007 90-91 20.75245809664366 27.891565531968997 28.95902423917655 22.39695213221079 92-93 21.04761617031347 27.817094959596034 29.107512560937437 22.027776309153058 94-95 20.598757319879358 27.70871202012726 29.10517464489459 22.5873560150988 96-97 20.341292259767812 27.803499107935597 29.33494626633703 22.52026236595956 98-99 20.364015686511465 28.107447400421453 28.70676915097973 22.82176776208735 100-101 19.884428236607377 27.97231145625038 29.268528096653917 22.87473221048833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1797.0 1 1286.0 2 934.5 3 1154.0 4 945.5 5 863.0 6 1636.5 7 3512.5 8 5914.5 9 7048.5 10 6000.5 11 4621.0 12 4339.5 13 4449.0 14 4593.5 15 4641.5 16 4641.5 17 4685.5 18 4808.0 19 4965.5 20 5102.5 21 5381.0 22 5904.0 23 6548.0 24 7167.0 25 8119.0 26 9333.5 27 10590.5 28 11962.5 29 13729.5 30 15891.5 31 17845.0 32 20092.5 33 21882.0 34 23163.5 35 24238.5 36 26398.0 37 29122.5 38 30841.5 39 31697.5 40 32650.0 41 35032.5 42 36787.0 43 38381.5 44 41013.0 45 42745.5 46 41841.5 47 44530.5 48 51701.5 49 49749.0 50 41513.0 51 36334.5 52 33005.5 53 32086.0 54 31846.0 55 29637.0 56 26673.5 57 24253.5 58 22796.0 59 20822.5 60 17538.0 61 14989.0 62 12925.0 63 9606.0 64 6503.0 65 4793.0 66 3472.5 67 2766.5 68 2205.5 69 1553.5 70 1111.0 71 884.0 72 736.0 73 653.5 74 530.5 75 375.5 76 256.5 77 153.5 78 83.0 79 52.5 80 34.5 81 20.5 82 11.0 83 5.5 84 4.0 85 1.0 86 0.5 87 0.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04456126040392139 2 0.0 3 0.0 4 0.0 5 0.011161294978759971 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006755520645038931 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013007524223366883 24-25 2.517585333554881E-4 26-27 0.0026015048446733765 28-29 0.002937182889147361 30-31 0.0014266316890144326 32-33 4.195975555924801E-5 34-35 0.0 36-37 3.356780444739841E-4 38-39 5.454768222702242E-4 40-41 4.195975555924801E-5 42-43 0.011958530334385684 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.937182889147361E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.035170667109762E-4 84-85 1.2587926667774406E-4 86-87 0.0 88-89 0.0 90-91 0.008098232822934866 92-93 0.0022658268001993927 94-95 0.0 96-97 0.0 98-99 0.0017623097334884166 100-101 0.012629886423333651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.7607565033814 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03137936265259 30.64626139884969 2 14.851445954297452 12.998210202582792 3 5.854002579603795 7.685267443686247 4 2.932323711914574 5.132828157847408 5 1.6605986617958752 3.633452684434515 6 1.0237380606447326 2.6879731197070824 7 0.7005139151186051 2.1458513206714964 8 0.5002991205411977 1.7514774394287373 9 0.36092817896992396 1.4215041139600535 >10 1.87314821074221 14.80861905592933 >50 0.13522620904999175 4.08463202801398 >100 0.06797141114065361 5.586743850630819 >500 0.004595249197315054 1.361138640220851 >1k 0.003254968181431497 2.6141067673899863 >5k 1.9146871655479394E-4 0.764734525656861 >10k+ 3.829374331095879E-4 2.677199250990251 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17457 1.4649829055955852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14495 1.2164133136625999 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9127 0.7659333779785132 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4875 0.40910761670266815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3391 0.28457106220282 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2578 0.21634449966348276 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2495 0.20937918024064756 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2439 0.2046796876180118 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2103 0.17648273188219715 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1371 0.11505364974345805 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1355 0.11371093756556212 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1306 0.10959888152075581 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1290 0.10825616934285988 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1270 0.10657777912048995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.1959755559248016E-5 0.0 18-19 0.0 0.0 0.0 8.391951111849603E-5 0.0 20-21 0.0 0.0 0.0 8.391951111849603E-5 0.0 22-23 0.0 0.0 0.0 1.6783902223699206E-4 0.0 24-25 0.0 0.0 0.0 1.6783902223699206E-4 0.0 26-27 0.0 0.0 0.0 7.133158445072163E-4 0.0 28-29 0.0 0.0 0.0 0.0020979877779624006 0.0 30-31 0.0 0.0 0.0 0.005370848711583746 0.0 32-33 0.0 0.0 0.0 0.008475870622968099 0.0 34-35 0.0 0.0 0.0 0.01141305351211546 0.0 36-37 0.0 0.0 0.0 0.013301242512281621 0.0 38-39 0.0 0.0 0.0 0.015357270534684775 0.0 40-41 0.0 0.0 0.0 0.017665057090443415 0.0 42-43 0.0 0.0 0.0 0.0210637972907425 0.0 44-45 0.0 0.0 0.0 0.025091933824430313 0.0 46-47 0.0 0.0 0.0 0.031092178869402777 0.0 48-49 0.0 0.0 0.0 0.0391484519367784 0.0 50-51 0.0 0.0 0.0 0.050687384715571604 0.0 52-53 0.0 0.0 0.0 0.08161172456273738 0.0 54-55 0.0 0.0 0.0 0.11748731556589444 0.0 56-57 0.0 0.0 0.0 0.15911139308066846 0.0 58-59 0.0 0.0 0.0 0.2234776581085549 0.0 60-61 0.0 0.0 0.0 0.3250202665619351 0.0 62-63 0.0 0.0 0.0 0.39547069614591257 0.0 64-65 0.0 0.0 0.0 0.4644525342853163 0.0 66-67 0.0 0.0 0.0 0.6001084240083651 0.0 68-69 0.0 0.0 0.0 0.7810388899798425 0.0 70-71 0.0 0.0 0.0 0.9388495306381743 0.0 72-73 0.0 0.0 0.0 1.0824777739174802 0.0 74-75 0.0 0.0 0.0 1.331970480472769 0.0 76-77 0.0 0.0 0.0 1.5192368695336929 0.0 78-79 0.0 0.0 0.0 1.685733179592789 0.0 80-81 0.0 0.0 0.0 1.8976299451669916 0.0 82-83 0.0 0.0 0.0 2.2805546744006886 0.0 84-85 0.0 0.0 0.0 2.6555490098336882 0.0 86-87 0.0 0.0 0.0 3.0638174314251714 0.0 88-89 0.0 0.0 0.0 3.591587236849393 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11820 0.0 30.520601 1 GTATCAA 16735 0.0 28.82764 1 GTGTAAG 395 0.0 26.472464 1 GGACCGA 235 1.7644197E-10 24.254398 6 GAGTACT 11725 0.0 23.577919 12-13 TATCAAC 20590 0.0 23.461725 2 ATCAACG 20600 0.0 23.404219 3 TCAACGC 20735 0.0 23.25184 4 CAACGCA 20995 0.0 22.963892 5 AACGCAG 21290 0.0 22.644749 6 GTACTTT 12195 0.0 22.570892 14-15 GTACATG 10320 0.0 22.521532 1 AGTACTT 11810 0.0 22.221724 12-13 TACATGG 10495 0.0 21.769978 2 CATGGGG 3245 0.0 21.517805 4 AGAGTAC 18095 0.0 20.987234 10-11 ATGGGGA 1320 0.0 20.871294 5 CGTAACA 115 0.003723271 20.652256 2 ACTTTTT 13540 0.0 20.521746 16-17 ACGCAGA 23530 0.0 20.388945 7 >>END_MODULE