FastQCFastQC Report
Thu 26 May 2016
SRR937517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330488
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157481.1836258575800758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118960.894108026528612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74230.5579155918730572No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45820.344384917413761No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32640.24532352039251765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28550.2145829199511758No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27130.20391014424782486No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25080.1885022638310154No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20900.15708521985917948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.14520987787939463No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17100.12852427079387413No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15630.11747569312913757No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14320.10762968174083494No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13680.10281941663509929No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13550.10184233153549675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA179650.029.8298591
GGTATCA124600.028.6981751
CTAGACG700.00836407227.1614323
GTACATG118700.025.3575631
GAGTACT107050.025.17970712-13
TATCAAC215450.024.6662062
TAAGGTG6000.024.5603125
ATCAACG216600.024.490433
TCAACGC217600.024.3578784
TACATGG120350.024.2114892
CAACGCA219400.024.1147085
GTACTTT112450.023.82270814-15
AACGCAG223350.023.7350756
AGTACTT107600.023.70464712-13
GTAAGGT5500.023.3359014
AGGTAAG4500.023.238992
ACATGGG119100.022.9880143
TGTATCG1050.002186724522.6362345
GGTAAGG5650.021.8733653
AGAGTAC175650.021.74181210-11
Produced by FastQC (version 0.11.5)