##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937517_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330488 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.560250073657183 31.0 31.0 34.0 30.0 34.0 2 31.600472157584285 33.0 31.0 34.0 30.0 34.0 3 31.60407008556259 33.0 31.0 34.0 30.0 34.0 4 35.295340506641175 37.0 35.0 37.0 33.0 37.0 5 35.306388332701985 37.0 35.0 37.0 33.0 37.0 6 35.394325240062294 37.0 35.0 37.0 33.0 37.0 7 35.3402338089483 37.0 35.0 37.0 33.0 37.0 8 35.332465982406454 37.0 35.0 37.0 33.0 37.0 9 36.89218091407063 39.0 37.0 39.0 33.0 39.0 10-11 36.79371178094053 39.0 37.0 39.0 33.0 39.0 12-13 36.666549792256674 39.0 37.0 39.0 32.0 39.0 14-15 37.67837101875402 40.0 37.0 41.0 32.0 41.0 16-17 37.653235128764784 40.0 37.0 41.0 32.0 41.0 18-19 37.71659383624655 40.0 37.0 41.0 32.0 41.0 20-21 37.70148096036942 40.0 37.0 41.0 32.0 41.0 22-23 37.565121970284586 40.0 37.0 41.0 32.0 41.0 24-25 37.39285096896778 40.0 37.0 41.0 32.0 41.0 26-27 37.20609280203955 39.0 36.5 41.0 31.5 41.0 28-29 37.01737332467486 39.0 36.0 41.0 31.0 41.0 30-31 36.88695952161913 39.0 36.0 41.0 31.0 41.0 32-33 36.6208988731954 39.0 35.0 40.5 30.5 41.0 34-35 36.36241176169946 39.0 35.0 40.0 30.0 41.0 36-37 36.26606177582962 39.0 35.0 40.5 30.0 41.0 38-39 36.18070963435973 39.0 35.0 41.0 30.0 41.0 40-41 35.96176139882509 39.0 35.0 41.0 29.5 41.0 42-43 35.616301687801766 38.5 35.0 40.0 28.0 41.0 44-45 35.22505952703068 38.0 35.0 40.0 26.5 41.0 46-47 34.666533256970375 38.0 34.5 40.0 23.5 41.0 48-49 34.16821609815346 38.0 34.0 40.0 21.0 41.0 50-51 33.146766825405415 37.5 32.5 39.5 17.0 40.5 52-53 33.24052302613778 37.5 33.0 39.5 17.5 40.5 54-55 33.56493482090782 38.0 33.0 40.0 15.0 41.0 56-57 33.31790365640276 38.0 33.0 40.0 12.5 41.0 58-59 33.05391179777645 37.5 33.0 40.0 8.5 41.0 60-61 32.752640008778734 37.0 33.0 40.0 7.5 41.0 62-63 32.434483813457916 36.5 32.0 40.0 6.0 41.0 64-65 32.06827269392885 36.0 32.0 39.0 2.0 41.0 66-67 31.666238628232648 35.5 31.0 39.0 2.0 41.0 68-69 31.163127363794338 35.0 31.0 38.5 2.0 40.0 70-71 30.728803266170008 35.0 30.0 38.0 2.0 40.0 72-73 30.32619159285916 35.0 30.0 37.0 2.0 39.5 74-75 29.88769571766149 35.0 29.5 37.0 2.0 39.0 76-77 29.469860682696876 34.5 29.0 36.0 2.0 39.0 78-79 29.00691888991107 34.0 29.0 36.0 2.0 38.0 80-81 28.556911449032235 34.0 28.5 35.0 2.0 37.0 82-83 28.110854062569523 34.0 27.0 35.0 2.0 37.0 84-85 27.652189271906245 34.0 26.0 35.0 2.0 36.0 86-87 27.24389735194906 33.5 26.0 35.0 2.0 36.0 88-89 26.84678854675878 33.0 25.0 35.0 2.0 35.5 90-91 26.39421400268172 33.0 24.5 35.0 2.0 35.0 92-93 25.944283601204972 33.0 23.5 35.0 2.0 35.0 94-95 25.40901158071324 32.5 20.0 35.0 2.0 35.0 96-97 24.785598968198137 32.0 15.5 34.5 2.0 35.0 98-99 24.07773350830673 32.0 2.0 34.0 2.0 35.0 100-101 22.35569805965931 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12036616337292116 1101 2 0.15983920641322769 1101 3 0.19955143527925046 1101 4 0.15943151921761256 1101 5 0.20314109215411946 1101 6 0.1639660969203831 1101 7 0.2594716350367463 1101 8 0.14147251901102464 1101 9 0.028727652584656482 1101 10-11 0.16950656220932814 1101 12-13 0.20551478235893228 1101 14-15 0.31762776882816013 1101 16-17 0.2447629536560214 1101 18-19 0.349122308811765 1101 20-21 0.44699058693418436 1101 22-23 0.3754025307028357 1101 24-25 0.4517200398531642 1101 26-27 0.34155503576236157 1101 28-29 0.3733324603592365 1101 30-31 0.26939146399565317 1101 32-33 0.389747978647236 1101 34-35 0.17288770103984774 1101 36-37 0.1611317460072641 1101 38-39 0.30004976583425247 1101 40-41 0.4212903158825654 1101 42-43 0.4377472740015662 1101 44-45 0.33533346336992764 1101 46-47 0.3493793383437662 1101 48-49 0.28115601001294266 1101 50-51 0.23551921729859515 1101 52-53 0.22083529185668738 1101 54-55 0.20088880892984662 1101 56-57 0.4463662826518302 1101 58-59 0.4141641929898938 1101 60-61 0.397097631495825 1101 62-63 0.43655456896944855 1101 64-65 0.3676356633849238 1101 66-67 0.5062127980418332 1101 68-69 0.26683124741289177 1101 70-71 0.3323135002312476 1101 72-73 0.48036479012127486 1101 74-75 0.468369204601256 1101 76-77 0.5868848651210605 1101 78-79 0.6574398016919467 1101 80-81 0.7199785347882042 1101 82-83 0.7433905786063129 1101 84-85 0.785535337947497 1101 86-87 1.0314975779671336 1101 88-89 0.8424070724292001 1101 90-91 0.9487322197507702 1101 92-93 0.8335345567504078 1101 94-95 0.8621600711710222 1101 96-97 0.8861884085785157 1101 98-99 0.9242216426347447 1101 100-101 1.2385712190491525 1102 1 -0.14544237237414492 1102 2 -0.35842749892827186 1102 3 -0.1405413843145844 1102 4 -0.3309432160518426 1102 5 -0.3086592344627732 1102 6 -0.17158984714945547 1102 7 -0.2522722687106054 1102 8 -0.16655264082066168 1102 9 -0.14733315696431504 1102 10-11 -0.1802955604031311 1102 12-13 -0.10037584468987149 1102 14-15 -0.015674637885361165 1102 16-17 -0.08530527033482826 1102 18-19 0.05933266280567295 1102 20-21 0.07796713965579016 1102 22-23 0.1992247033011907 1102 24-25 0.12278225997388148 1102 26-27 0.1499586658985308 1102 28-29 0.25989726123022194 1102 30-31 0.12177533023371723 1102 32-33 0.10016614363247811 1102 34-35 -0.04296593326842668 1102 36-37 0.03956807756711811 1102 38-39 0.20921868080657902 1102 40-41 -8.107605227465342E-4 1102 42-43 -0.03458684474870921 1102 44-45 0.11370423314475175 1102 46-47 0.17029970660232152 1102 48-49 0.05238054521961999 1102 50-51 -0.09547461989361494 1102 52-53 -0.01588606261005765 1102 54-55 0.18243276910007467 1102 56-57 0.1332749848860928 1102 58-59 0.11138894276241018 1102 60-61 0.2999694363521712 1102 62-63 0.43988033951909244 1102 64-65 0.36695163542778886 1102 66-67 0.5310472048666135 1102 68-69 0.4607381735188021 1102 70-71 0.49352019468551234 1102 72-73 0.46271059078960874 1102 74-75 0.42873770156753466 1102 76-77 0.2917624632551359 1102 78-79 0.27778859038830106 1102 80-81 0.08724909834141314 1102 82-83 0.17575505444977324 1102 84-85 0.2355470595999698 1102 86-87 0.39999891503020635 1102 88-89 0.5194157464520295 1102 90-91 0.6574197522833245 1102 92-93 0.21634465405999137 1102 94-95 0.20620368366511244 1102 96-97 0.40761598664671084 1102 98-99 0.4052367377390631 1102 100-101 0.6551633174633515 1103 1 -0.1084880928865175 1103 2 -0.24948584045576538 1103 3 -0.1659375282124813 1103 4 -0.20142563836129312 1103 5 -0.200969506778101 1103 6 -0.27547797539064334 1103 7 -0.0932285342068937 1103 8 -0.15390716937458393 1103 9 -0.0801019503864211 1103 10-11 -0.17812244147281575 1103 12-13 -0.12422968749627472 1103 14-15 -0.1452335584065878 1103 16-17 -0.3555779117457476 1103 18-19 -0.37884272473969105 1103 20-21 -0.24290116848299448 1103 22-23 -0.2439009544477173 1103 24-25 -0.31502259487785267 1103 26-27 -0.5357482829190374 1103 28-29 -0.6151262731276148 1103 30-31 -0.4585178816616775 1103 32-33 -0.48500241671699484 1103 34-35 -0.31541149951187464 1103 36-37 -0.36133742747340847 1103 38-39 -0.2711591965476785 1103 40-41 -0.1562442760103906 1103 42-43 -0.23445594519470347 1103 44-45 -0.2249812822295354 1103 46-47 -0.4013681874094104 1103 48-49 -0.3014636198706526 1103 50-51 -0.493084344348496 1103 52-53 -0.6444261637971209 1103 54-55 -0.39389813037630006 1103 56-57 -0.4429297510225041 1103 58-59 -0.2876687512548344 1103 60-61 -0.3144535438952687 1103 62-63 -0.39999848910846936 1103 64-65 -0.362334927065298 1103 66-67 -0.37525363662497213 1103 68-69 -0.36537795674566453 1103 70-71 -0.13531241188097098 1103 72-73 -0.20981808813032288 1103 74-75 -0.32379168386837875 1103 76-77 -0.12401073890432102 1103 78-79 -0.05123046652155949 1103 80-81 -0.1919091460983644 1103 82-83 -0.053323241107602115 1103 84-85 -0.05818540609033107 1103 86-87 0.122428972859705 1103 88-89 0.05555714436765058 1103 90-91 0.17072437984652922 1103 92-93 0.0631898467039953 1103 94-95 0.18571743762907644 1103 96-97 0.2818134407823685 1103 98-99 0.40279052320898145 1103 100-101 0.6240814668107362 1104 1 0.13273009538612968 1104 2 0.10156376820205892 1104 3 0.07927407814822729 1104 4 -0.0631365966574009 1104 5 0.0317626680574179 1104 6 0.08560750551544771 1104 7 0.021043530952681522 1104 8 0.1664878356905959 1104 9 0.23319126053931427 1104 10-11 0.1361911465011545 1104 12-13 0.24979713330548492 1104 14-15 0.3465366047219831 1104 16-17 0.261880643553944 1104 18-19 0.2457524466426193 1104 20-21 0.28837721845918907 1104 22-23 0.306129331231503 1104 24-25 0.12873808463566405 1104 26-27 0.06412180299892611 1104 28-29 0.18395337350307273 1104 30-31 0.23527206710677007 1104 32-33 0.15834917084362843 1104 34-35 0.2958118574665036 1104 36-37 0.16498287944155265 1104 38-39 0.19146876468213492 1104 40-41 0.17839629189220574 1104 42-43 0.24044609220895552 1104 44-45 0.4044814321277599 1104 46-47 0.41825565278459464 1104 48-49 0.47503954930263603 1104 50-51 0.44899885863782885 1104 52-53 0.5185668819784865 1104 54-55 0.6553323799703392 1104 56-57 0.5677803493362177 1104 58-59 0.6031787621384339 1104 60-61 0.6980507335569328 1104 62-63 0.6925627532782315 1104 64-65 0.5860721290274 1104 66-67 0.5520283582469823 1104 68-69 0.7239821138167635 1104 70-71 0.7099902585667941 1104 72-73 0.7107929635638861 1104 74-75 0.8381847493933918 1104 76-77 0.7024737770010248 1104 78-79 0.6240255500514245 1104 80-81 0.8680468991980206 1104 82-83 0.7130446721093442 1104 84-85 0.7253529249635768 1104 86-87 0.6715136074984649 1104 88-89 0.5563124981943552 1104 90-91 0.8251491571084415 1104 92-93 0.8418756929205067 1104 94-95 0.9499922697214309 1104 96-97 1.0790868638095006 1104 98-99 0.9688251621957811 1104 100-101 1.162660791761958 1105 1 -0.10638542898376713 1105 2 0.037546212782654464 1105 3 -0.011276590198459502 1105 4 -0.22400797122824656 1105 5 -0.1478143218586183 1105 6 -0.07616129161465324 1105 7 -0.15199333311612406 1105 8 -0.0629860797150883 1105 9 -0.08401120091852476 1105 10-11 0.007404651381300198 1105 12-13 0.033540260065940686 1105 14-15 0.03418827838229532 1105 16-17 -0.007466345707037192 1105 18-19 -0.02094138545574964 1105 20-21 -0.13326419013587554 1105 22-23 0.009160492486280702 1105 24-25 -0.025350023841106406 1105 26-27 -0.02659783047968034 1105 28-29 -0.013610214799996356 1105 30-31 -0.03665949141836222 1105 32-33 -0.039551384410088986 1105 34-35 0.07002145900163725 1105 36-37 -0.026766343164709383 1105 38-39 -0.12606488384727044 1105 40-41 -0.16246764590088247 1105 42-43 -0.002061643195879981 1105 44-45 0.044569132159743674 1105 46-47 0.06689526191064488 1105 48-49 0.22096492721039596 1105 50-51 0.04093323003744587 1105 52-53 -0.06578891196496528 1105 54-55 0.09993339491710174 1105 56-57 0.02534717437156786 1105 58-59 0.2001514541363889 1105 60-61 0.10569635761047635 1105 62-63 0.037191511644607544 1105 64-65 -0.07695032387622192 1105 66-67 -0.08423306183077628 1105 68-69 0.11141723467403963 1105 70-71 -0.1269221685585613 1105 72-73 -0.038425018795923194 1105 74-75 0.0014902237095384407 1105 76-77 0.019050470216601667 1105 78-79 0.05552897366647258 1105 80-81 -0.04276031871498276 1105 82-83 0.07460076968911622 1105 84-85 0.07407036979462944 1105 86-87 0.06430012573783728 1105 88-89 -0.04007700400392267 1105 90-91 -0.004770964962602875 1105 92-93 -0.13652913751710827 1105 94-95 -0.11490999252324485 1105 96-97 -0.06731477613485737 1105 98-99 -0.12163822997672114 1105 100-101 0.39462217446316217 1106 1 0.10441118335746324 1106 2 0.1473652979163873 1106 3 -0.11063882086181209 1106 4 -0.05325897458862272 1106 5 0.047025657144182276 1106 6 -0.014096373888136782 1106 7 0.045884213517915384 1106 8 -0.03188666127145012 1106 9 0.04920901756810281 1106 10-11 -0.001599345526990703 1106 12-13 -0.20442292869807943 1106 14-15 -0.22763546085560193 1106 16-17 -0.2539710142777807 1106 18-19 -0.16662993247418711 1106 20-21 -0.1870567063694395 1106 22-23 -0.19688467629733708 1106 24-25 -0.32055828053602653 1106 26-27 -0.18379763869420884 1106 28-29 -0.018802475595158796 1106 30-31 0.05719614041968413 1106 32-33 0.10335958001453349 1106 34-35 0.11275138456077372 1106 36-37 -0.10939053871975091 1106 38-39 -0.09432294276343356 1106 40-41 0.007375949745920707 1106 42-43 -0.015158234457246067 1106 44-45 0.016136523670752467 1106 46-47 0.07305099666803727 1106 48-49 0.15895250248825477 1106 50-51 -0.04008083560773912 1106 52-53 -0.0085913770695214 1106 54-55 0.007733359370661219 1106 56-57 0.005205980589721548 1106 58-59 -0.015523080969863656 1106 60-61 -0.019235752814964258 1106 62-63 0.19190616876608857 1106 64-65 0.054287353700999574 1106 66-67 0.1952876398394885 1106 68-69 0.35175934853012336 1106 70-71 0.3418504871327954 1106 72-73 0.4900828158883108 1106 74-75 0.43206553877089604 1106 76-77 0.3297487098409668 1106 78-79 0.3098269933988824 1106 80-81 0.16133289563421016 1106 82-83 0.1022616896806845 1106 84-85 0.14141021446493696 1106 86-87 0.0692727012430332 1106 88-89 0.2992807626874061 1106 90-91 0.37520150720990486 1106 92-93 0.24545336205923007 1106 94-95 0.11618529261297184 1106 96-97 0.19577478850386854 1106 98-99 0.2645368899925238 1106 100-101 0.34494415911206033 1107 1 0.009225486134692318 1107 2 -0.049213246036813985 1107 3 0.019034175216397387 1107 4 -0.02756513753570289 1107 5 -0.09708366392533208 1107 6 0.15909250214756554 1107 7 0.11298726160451622 1107 8 0.12060231163340518 1107 9 0.14178959295205118 1107 10-11 0.13478395535988597 1107 12-13 0.12415629239870896 1107 14-15 0.07021366779228089 1107 16-17 0.04564150911659226 1107 18-19 -0.013388577810836466 1107 20-21 0.041761395935665746 1107 22-23 0.06873429877061454 1107 24-25 0.027766259335500365 1107 26-27 0.02187061737892293 1107 28-29 -0.09634011819501609 1107 30-31 -0.1283035821395444 1107 32-33 -0.150644501656501 1107 34-35 0.11316519452794438 1107 36-37 -0.006102630435087519 1107 38-39 0.15368559676952742 1107 40-41 0.06480148746376102 1107 42-43 0.17068005989330715 1107 44-45 0.34342729100610114 1107 46-47 0.4763370239983047 1107 48-49 0.311366237217257 1107 50-51 0.16970200820721004 1107 52-53 0.18937907313691227 1107 54-55 0.31328029388523504 1107 56-57 0.1971338647981682 1107 58-59 0.19276440410006757 1107 60-61 0.31698954790262235 1107 62-63 0.33543239397275926 1107 64-65 0.24083570833614232 1107 66-67 0.3128378848338009 1107 68-69 0.26305353920247043 1107 70-71 0.3007892526624225 1107 72-73 0.4280630470576092 1107 74-75 0.3658888607244535 1107 76-77 0.15571340432694925 1107 78-79 0.3322526721883001 1107 80-81 0.240008515643801 1107 82-83 0.33896569329211346 1107 84-85 0.41047603846403646 1107 86-87 0.5185997898077694 1107 88-89 0.6083779986249205 1107 90-91 0.44221991234867275 1107 92-93 0.5594316746550376 1107 94-95 0.5922689574645155 1107 96-97 0.5104255422963426 1107 98-99 0.7037397415193887 1107 100-101 0.797755715695871 1108 1 -0.1061772650673305 1108 2 -0.24414003166792853 1108 3 -0.1059651030520925 1108 4 -0.25632785107681144 1108 5 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96-97 -1.4812295929127721 2316 98-99 -1.6140250131916218 2316 100-101 -1.8522452174856632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5075.0 3 953.0 4 785.0 5 980.0 6 1124.0 7 1469.0 8 1813.0 9 2373.0 10 3196.0 11 4296.0 12 6269.0 13 8609.0 14 10428.0 15 13408.0 16 17601.0 17 23895.0 18 19209.0 19 11491.0 20 10656.0 21 10929.0 22 11224.0 23 12712.0 24 14088.0 25 15752.0 26 17865.0 27 20755.0 28 24651.0 29 29373.0 30 34842.0 31 42972.0 32 53537.0 33 68717.0 34 92596.0 35 129196.0 36 175530.0 37 229638.0 38 179771.0 39 22705.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.073936581826516 19.397586181408982 15.771880416271328 19.756596820493172 2 15.146239921832475 21.663216177541614 40.10074020908004 23.089803691545875 3 19.03731856986573 27.754258533087743 30.66611276018086 22.542310136865666 4 12.082611091968626 17.68333369775264 38.05163344042234 32.18242176985639 5 12.379856800613927 39.240672364689544 32.753731129508154 15.625739705188376 6 28.670296151826687 37.488860313033676 17.72270522448494 16.1181383106547 7 26.311911565614977 36.20156446231119 20.38871230573237 17.09781166634146 8 27.24472968320467 32.41933211640614 19.872200044146883 20.4637381562423 9 28.164223439462234 16.301356550457996 20.62413223937424 34.91028777070554 10-11 24.841587317492504 27.70825320564134 25.799540950728485 21.65061852613767 12-13 27.000643780138343 25.90859075435286 26.987948993326004 20.102816472182784 14-15 23.656935733356534 25.921749541738087 27.843568382997365 22.57774634190802 16-17 20.471071814946402 29.749404078483842 27.815145296363596 21.964378810206153 18-19 20.505985190218055 26.93017347127474 32.52163636000704 20.042204978500166 20-21 22.311432077431657 25.237793206726234 32.5383290182664 19.912445697575702 22-23 21.648494399170186 25.631390409155674 32.7382165252445 19.981898666429636 24-25 21.186138754063588 25.830983305765702 32.844987059784096 20.13789088038661 26-27 20.478417969375315 26.00879801515452 33.07692858194813 20.435855433522025 28-29 20.36084493461098 26.757706563008497 32.697447718917616 20.184000783462906 30-31 20.415949371856932 26.225943156348297 32.65368315973857 20.7044243120562 32-33 20.265318176886822 26.485445773111373 32.436156683583064 20.813079366418748 34-35 21.025470855260878 26.343261432581176 32.300045650474054 20.331222061683885 36-37 20.93936471002094 26.044727240815785 32.42659047535829 20.589317573804994 38-39 20.50855047035213 26.625368924861537 32.17514370039968 20.69093690438665 40-41 20.623637659131205 26.75268400922513 31.987236363033805 20.63644196860986 42-43 20.554774532462588 26.89943958402455 31.27683027730065 21.268955606212213 44-45 20.68668855383475 26.840345212571208 31.236675219637583 21.23629101395646 46-47 21.192707715426902 27.41039532829781 30.475361893086415 20.921535063188877 48-49 21.112737038564614 27.667346542895295 29.747692469947022 21.47222394859307 50-51 20.973122064799213 28.07723559319544 29.661824939134668 21.287817402870683 52-53 21.45463052750785 28.303884183504707 28.82919945177942 21.412285837208024 54-55 20.85943651041804 28.15767763031497 29.107902340527325 21.87498351873966 56-57 21.348283719425698 28.143439053239288 28.798296692309922 21.709980535025093 58-59 21.699768444436078 27.799393813889566 28.878894557502967 21.62194318417139 60-61 21.35292248185781 27.96661354797702 29.692381032711673 20.988082937453495 62-63 21.22698471691442 28.157598747741165 29.59835914027325 21.01705739507117 64-65 20.9821916064757 28.31878733929934 29.367130647351335 21.331890406873633 66-67 21.153298646678156 28.164504525132262 28.95263674817127 21.729560080018317 68-69 21.091773665275664 28.100022518095436 29.263055788393167 21.545148028235733 70-71 21.460932042710308 28.320489236753758 28.824600414601036 21.393978305934898 72-73 21.1995955023172 28.34037312463161 29.013933325675247 21.446098047375944 74-75 20.667170289942398 29.070912969077632 28.772617856884626 21.489298884095344 76-77 20.84655012538219 28.463452352884016 28.9043803543544 21.785617167379385 78-79 20.83531220917116 28.674108620792904 28.826869675160548 21.663709494875384 80-81 21.0672404567174 28.855996044523955 28.432356426650664 21.64440707210798 82-83 20.924043734575072 28.48230961219856 28.857196767549247 21.73644988567712 84-85 20.837853418577655 28.710125505556082 28.57660334844652 21.875417727419748 86-87 20.881989439444002 28.681263561261694 28.240110681736535 22.196636317557772 88-89 20.773911654959335 28.41616591019675 28.14267680381544 22.667245631028475 90-91 20.759928899065294 28.193026986721296 28.469183318395107 22.5778607958183 92-93 21.242557800502166 28.126533491357087 28.651258220831792 21.97965048730895 94-95 20.813558581595753 27.928364243583264 28.69655732901697 22.561519845804014 96-97 20.450202860915063 27.93982633870505 28.94957435735605 22.660396443023835 98-99 20.581269096536346 28.253326699956578 28.416775915268527 22.74862828823855 100-101 20.536723525482543 27.666052417748503 29.167096540500893 22.630127516268058 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3367.0 1 2079.5 2 975.5 3 1200.5 4 1104.5 5 1097.5 6 1786.5 7 3460.0 8 5753.5 9 6955.5 10 5904.5 11 4611.5 12 4467.5 13 4614.0 14 4674.0 15 4676.0 16 4663.0 17 4711.5 18 4782.0 19 5010.0 20 5328.0 21 5634.5 22 6163.0 23 6879.0 24 7684.0 25 8744.0 26 10112.5 27 11671.5 28 13300.0 29 15427.5 30 17642.5 31 19454.0 32 22389.5 33 24677.5 34 25906.5 35 27260.5 36 29664.0 37 32768.5 38 34940.5 39 35798.0 40 36780.5 41 39177.5 42 40671.5 43 42420.5 44 45200.0 45 47339.5 46 47176.0 47 49934.0 48 57592.5 49 56192.5 50 47347.5 51 41147.0 52 37248.0 53 36273.5 54 36065.5 55 33385.5 56 29996.5 57 27320.0 58 25565.0 59 23463.0 60 20037.0 61 17292.0 62 14743.0 63 10927.5 64 7592.5 65 5811.0 66 4308.5 67 3364.5 68 2662.0 69 1924.5 70 1348.5 71 1013.0 72 869.0 73 748.5 74 583.5 75 429.0 76 298.5 77 198.0 78 137.0 79 87.5 80 47.5 81 27.0 82 20.0 83 17.0 84 12.0 85 6.0 86 4.5 87 3.0 88 2.5 89 4.0 90 3.0 91 2.5 92 3.0 93 2.5 94 2.5 95 1.0 96 1.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3473913331048457 2 0.30860857068985215 3 0.3119907883423225 4 0.3139449585415276 5 0.29658290792551306 6 0.22751050742284032 7 0.23532718821966075 8 0.23307237645134718 9 0.23126852703669631 10-11 0.23897246724510104 12-13 0.2383711841068841 14-15 0.21928044446849576 16-17 0.2354023486119379 18-19 0.21943076525305 20-21 0.22315120467076743 22-23 0.22258750172868902 24-25 0.2137561556361275 26-27 0.22769840840353314 28-29 0.22908887566065986 30-31 0.2377323207725286 32-33 0.23870940587213113 34-35 0.2262327807541293 36-37 0.22191105819819495 38-39 0.21138860327939826 40-41 0.21120070229870544 42-43 0.21146376367167535 44-45 0.23104304585986496 46-47 0.21950592564532714 48-49 0.22852517271858144 50-51 0.2384463444991612 52-53 0.24690188863033713 54-55 0.24224194430915572 56-57 0.204323526405349 58-59 0.188878065792401 60-61 0.19872407718070362 62-63 0.1989871385536735 64-65 0.19774699208110108 66-67 0.20984781523771728 68-69 0.2004527662030774 70-71 0.20334644130574644 72-73 0.21913012368394152 74-75 0.20864524896128336 76-77 0.2214976760406708 78-79 0.21980656721443562 80-81 0.20357192248257783 82-83 0.2171007930924593 84-85 0.1968074871776371 86-87 0.20421078581693333 88-89 0.21270391014424783 90-91 0.21029877759138 92-93 0.21341793387088046 94-95 0.21247842896741648 96-97 0.20526303130881302 98-99 0.21484598132414573 100-101 0.19383865168269085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1330488.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.879098666296926 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14841173730392 31.93076590308781 2 14.199627435363862 12.745329613927032 3 5.562460882328379 7.4891469229629735 4 2.8497779421956473 5.115818617793403 5 1.6376116072934925 3.674726645039887 6 1.041366543238733 2.8041355105075 7 0.6970881169088544 2.1899280464498925 8 0.46894689221247676 1.6836731075885645 9 0.3433783348789383 1.3869459153810562 >10 1.8429172968499261 14.929251741253536 >50 0.13425700493469145 4.133198618621392 >100 0.0658052946000254 5.386663397430983 >500 0.005344583610238528 1.6057134246315732 >1k 0.0025052735672993097 2.2962106736142105 >5k 1.67018237819954E-4 0.5564004645243287 >10k+ 3.34036475639908E-4 2.072091397185847 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15748 1.1836258575800758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11896 0.894108026528612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7423 0.5579155918730572 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4582 0.344384917413761 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3264 0.24532352039251765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2855 0.2145829199511758 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2713 0.20391014424782486 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2508 0.1885022638310154 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2090 0.15708521985917948 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1932 0.14520987787939463 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1710 0.12852427079387413 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1563 0.11747569312913757 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1432 0.10762968174083494 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1368 0.10281941663509929 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1355 0.10184233153549675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.758019613855969E-5 0.0 14-15 0.0 0.0 0.0 7.516039227711938E-5 0.0 16-17 0.0 0.0 0.0 7.516039227711938E-5 0.0 18-19 0.0 0.0 0.0 7.516039227711938E-5 0.0 20-21 0.0 0.0 0.0 1.1274058841567906E-4 0.0 22-23 0.0 0.0 0.0 2.2548117683135813E-4 0.0 24-25 0.0 0.0 0.0 3.3822176524703716E-4 0.0 26-27 0.0 0.0 0.0 8.643445111868727E-4 0.0 28-29 0.0 0.0 0.0 0.002029330591482223 0.0 30-31 0.0 0.0 0.0 0.0051860670671212365 0.0 32-33 0.0 0.0 0.0 0.008681025308007288 0.0 34-35 0.0 0.0 0.0 0.011424379626122146 7.516039227711938E-5 36-37 0.0 0.0 0.0 0.013867092375128525 7.516039227711938E-5 38-39 0.0 0.0 0.0 0.016347385320273463 7.516039227711938E-5 40-41 0.0 0.0 0.0 0.019917503953436635 7.516039227711938E-5 42-43 0.0 0.0 0.0 0.022999080036798528 7.516039227711938E-5 44-45 0.0 0.0 0.0 0.0263812976892689 7.516039227711938E-5 46-47 0.0 0.0 0.0 0.03156736475639013 7.516039227711938E-5 48-49 0.0 0.0 0.0 0.03957194653390335 7.516039227711938E-5 50-51 0.0 0.0 0.0 0.05302565675150772 7.516039227711938E-5 52-53 0.0 0.0 0.0 0.08466818190017497 7.516039227711938E-5 54-55 0.0 0.0 0.0 0.11871583960171005 7.516039227711938E-5 56-57 0.0 0.0 0.0 0.161820324572638 7.516039227711938E-5 58-59 0.0 0.0 0.0 0.22792388958036447 7.516039227711938E-5 60-61 0.0 0.0 0.0 0.3342006842602113 7.516039227711938E-5 62-63 0.0 0.0 0.0 0.40834641124158955 7.516039227711938E-5 64-65 0.0 0.0 0.0 0.4845590490105886 7.516039227711938E-5 66-67 0.0 0.0 0.0 0.615187810788222 7.516039227711938E-5 68-69 0.0 0.0 0.0 0.7903866851861874 7.516039227711938E-5 70-71 0.0 0.0 0.0 0.9418724558207214 7.516039227711938E-5 72-73 0.0 0.0 0.0 1.0829485121248745 7.516039227711938E-5 74-75 0.0 0.0 0.0 1.322898064469578 7.516039227711938E-5 76-77 0.0 0.0 0.0 1.5112875877121779 7.516039227711938E-5 78-79 0.0 0.0 0.0 1.6708155203203638 7.516039227711938E-5 80-81 7.516039227711938E-5 0.0 0.0 1.873485518095616 7.516039227711938E-5 82-83 7.516039227711938E-5 0.0 0.0 2.223732946106992 7.516039227711938E-5 84-85 7.516039227711938E-5 0.0 0.0 2.5723644256844107 7.516039227711938E-5 86-87 7.516039227711938E-5 0.0 0.0 2.9411764705882355 7.516039227711938E-5 88-89 7.516039227711938E-5 0.0 0.0 3.4344917052991084 7.516039227711938E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17965 0.0 29.829859 1 GGTATCA 12460 0.0 28.698175 1 CTAGACG 70 0.008364072 27.161432 3 GTACATG 11870 0.0 25.357563 1 GAGTACT 10705 0.0 25.179707 12-13 TATCAAC 21545 0.0 24.666206 2 TAAGGTG 600 0.0 24.560312 5 ATCAACG 21660 0.0 24.49043 3 TCAACGC 21760 0.0 24.357878 4 TACATGG 12035 0.0 24.211489 2 CAACGCA 21940 0.0 24.114708 5 GTACTTT 11245 0.0 23.822708 14-15 AACGCAG 22335 0.0 23.735075 6 AGTACTT 10760 0.0 23.704647 12-13 GTAAGGT 550 0.0 23.335901 4 AGGTAAG 450 0.0 23.23899 2 ACATGGG 11910 0.0 22.988014 3 TGTATCG 105 0.0021867245 22.636234 5 GGTAAGG 565 0.0 21.873365 3 AGAGTAC 17565 0.0 21.741812 10-11 >>END_MODULE