FastQCFastQC Report
Thu 26 May 2016
SRR937517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330488
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192401.4460859474117767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160031.2027917576107412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101120.760021886706231No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54270.4078954488879269No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36280.27268190318138913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28880.21706321289632075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28140.2115013438678139No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27520.2068413995466325No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21470.1613693622189753No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16330.12273692058853594No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15700.11800181587507741No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.11424379626122144No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14810.1113125409624138No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14260.10717871938717223No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.10304489781193067No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13480.10131620878955691No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.10109072761272556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140300.030.2874131
GTATCAA198300.028.40391
GAGTACT132750.025.33398212-13
CCCTATA1751.4834404E-724.4337922
GTACTTT138500.024.07462314-15
TAACGTG1000.001651726723.7550745
TATCAAC240350.023.404212
ATCAACG240750.023.385063
TCAACGC241900.023.2542444
CAACGCA243900.023.0830365
AGTACTT133700.022.7913512-13
GTACATG112950.022.7663481
AACGCAG247950.022.7383386
TACATGG115600.021.9467322
ACTTTTT155050.021.6431616-17
ATGGGAG25750.021.5871355
AGAGTAC200350.021.43304610-11
TAAGGTG5000.020.9044675
GGACCGA3650.020.8208876
TACTTTT145850.020.69575514-15
Produced by FastQC (version 0.11.5)