##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330488 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0052183860358 31.0 31.0 34.0 30.0 34.0 2 32.204922554731795 33.0 31.0 34.0 30.0 34.0 3 32.23696042354384 34.0 31.0 34.0 30.0 34.0 4 35.93197608696959 37.0 35.0 37.0 35.0 37.0 5 35.81575482078756 37.0 35.0 37.0 35.0 37.0 6 35.916386318403475 37.0 35.0 37.0 35.0 37.0 7 35.86668275099061 37.0 35.0 37.0 35.0 37.0 8 35.85610167096584 37.0 35.0 37.0 35.0 37.0 9 37.48959479529316 39.0 37.0 39.0 35.0 39.0 10-11 37.418440451924404 39.0 37.0 39.0 34.0 39.0 12-13 37.32157110774393 39.0 37.0 39.0 34.0 39.0 14-15 38.36524418108243 40.0 38.0 41.0 33.5 41.0 16-17 38.37365913860178 40.0 38.0 41.0 33.5 41.0 18-19 38.495541861332086 40.0 38.0 41.0 34.0 41.0 20-21 38.436724344751696 40.0 38.0 41.0 34.0 41.0 22-23 38.31660338161637 40.0 38.0 41.0 34.0 41.0 24-25 38.156242296059794 40.0 37.5 41.0 33.0 41.0 26-27 38.0351769425955 40.0 37.0 41.0 33.0 41.0 28-29 37.9106936702924 40.0 37.0 41.0 33.0 41.0 30-31 37.82578234452322 40.0 37.0 41.0 33.0 41.0 32-33 37.59500461484809 40.0 36.0 41.0 33.0 41.0 34-35 37.40035272772096 40.0 35.0 41.0 33.0 41.0 36-37 37.16253434829927 39.5 35.0 41.0 32.5 41.0 38-39 36.966722360517345 39.0 35.0 41.0 32.0 41.0 40-41 36.73432605179453 39.0 35.0 41.0 31.0 41.0 42-43 36.3978141854718 39.0 35.0 41.0 30.5 41.0 44-45 35.89940382776846 39.0 35.0 41.0 29.5 41.0 46-47 35.27441773244103 39.0 35.0 41.0 26.5 41.0 48-49 34.73170596051975 39.0 35.0 40.0 23.5 41.0 50-51 34.40921714438612 38.0 34.0 40.0 22.5 41.0 52-53 34.144165148426744 38.0 34.0 40.0 20.5 41.0 54-55 33.88474229004696 38.0 34.0 40.0 19.5 41.0 56-57 33.60888222967813 38.0 33.5 40.0 18.0 41.0 58-59 33.3457791426905 37.5 33.0 40.0 16.0 41.0 60-61 32.98803446554948 37.0 33.0 40.0 12.5 41.0 62-63 32.61502621594482 36.0 32.5 39.5 8.0 41.0 64-65 32.25893544323586 36.0 32.0 39.0 7.0 41.0 66-67 31.81258981666877 35.0 31.5 39.0 3.5 40.0 68-69 31.396827329521198 35.0 31.0 38.5 2.0 40.0 70-71 30.979001689605617 35.0 31.0 37.5 2.0 40.0 72-73 30.535538839884314 35.0 30.5 37.0 2.0 39.0 74-75 30.098601039618547 34.5 30.0 36.5 2.0 39.0 76-77 28.801797911743662 33.5 28.5 35.0 2.0 37.5 78-79 29.412464824936414 34.0 29.5 36.0 2.0 37.5 80-81 29.25717105302716 34.0 29.0 35.0 2.0 37.0 82-83 28.91973245906765 34.0 29.0 35.0 2.0 37.0 84-85 28.55092417218344 34.0 29.0 35.0 2.0 36.0 86-87 28.191100182790073 34.0 29.0 35.0 2.0 36.0 88-89 27.766564223051994 34.0 27.5 35.0 2.0 35.5 90-91 27.302985445941637 33.0 26.5 35.0 2.0 35.0 92-93 26.923940689431248 33.0 26.0 35.0 2.0 35.0 94-95 26.493207755349918 33.0 25.0 35.0 2.0 35.0 96-97 25.99634645333141 33.0 24.5 35.0 2.0 35.0 98-99 25.503774179098194 33.0 21.5 35.0 2.0 35.0 100-101 23.729865282512883 30.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14139550680136992 1101 2 0.10142311221729727 1101 3 0.10524918405882744 1101 4 -0.009581306567163494 1101 5 -0.08359425381210173 1101 6 0.018304014026988114 1101 7 0.08398353983022133 1101 8 0.07267518766177972 1101 9 0.08518469346864066 1101 10-11 0.09777323075240929 1101 12-13 -0.03704499233607095 1101 14-15 0.08900847473242379 1101 16-17 0.04801837680893328 1101 18-19 0.10779812210163442 1101 20-21 0.11355747376622816 1101 22-23 0.10022075568830502 1101 24-25 0.06503461573631597 1101 26-27 -0.01487489835065503 1101 28-29 -0.006649295157714619 1101 30-31 0.046720961613033296 1101 32-33 0.07767635760384906 1101 34-35 0.03543509073251272 1101 36-37 0.025813134148705785 1101 38-39 -0.14141879091344833 1101 40-41 -0.17030044632128494 1101 42-43 -0.0455444279089221 1101 44-45 0.017949091028157227 1101 46-47 0.038207280430519575 1101 48-49 0.08551505798315873 1101 50-51 -0.04203802972929083 1101 52-53 -0.143328998837859 1101 54-55 -0.05791950369977883 1101 56-57 -0.08542877332302368 1101 58-59 -0.2044517839406481 1101 60-61 -0.18294175810672186 1101 62-63 -0.26575356030314623 1101 64-65 -0.3457488576935006 1101 66-67 -0.3234419950079257 1101 68-69 -0.14522430323657076 1101 70-71 -0.23874770335409679 1101 72-73 -0.25052728806138447 1101 74-75 -0.2397777105963108 1101 76-77 -0.27117343486614587 1101 78-79 -0.03953288612222394 1101 80-81 -0.2404612958447352 1101 82-83 -0.23069033912056014 1101 84-85 -0.17559493117071057 1101 86-87 -0.03332922159005136 1101 88-89 0.008600088156978103 1101 90-91 0.10901290457089985 1101 92-93 -0.009939095148808974 1101 94-95 0.03325626720313224 1101 96-97 0.041376440007752535 1101 98-99 0.08788944881193572 1101 100-101 0.38575736588171594 1102 1 -0.033016324518090556 1102 2 0.09227227640387525 1102 3 0.038516823159085334 1102 4 0.07890127585194762 1102 5 -0.012998913069623086 1102 6 0.06317847314226555 1102 7 -0.09413789648942128 1102 8 0.06604708986743191 1102 9 -0.009821882577071506 1102 10-11 0.06125015101736153 1102 12-13 0.004144298444359151 1102 14-15 -0.010004522394780224 1102 16-17 -0.05281465520508988 1102 18-19 0.1062884129972872 1102 20-21 -0.07962092183755232 1102 22-23 -0.0034550750589801282 1102 24-25 -0.043326895919769015 1102 26-27 -0.11684393729434817 1102 28-29 0.06888463604611417 1102 30-31 0.1844982581628969 1102 32-33 0.17011410370888314 1102 34-35 0.10403755188219321 1102 36-37 0.08780776205577467 1102 38-39 0.09706200345698335 1102 40-41 0.1367075124820758 1102 42-43 0.24623452159261205 1102 44-45 0.3130758636797566 1102 46-47 0.13087091668578665 1102 48-49 0.04026908208604851 1102 50-51 -0.10565322575902769 1102 52-53 -0.10414212390988098 1102 54-55 -4.2738104758655027E-4 1102 56-57 -0.0653829595423332 1102 58-59 -0.01384718507943461 1102 60-61 0.015489129838243798 1102 62-63 -0.10684212434112084 1102 64-65 0.04813502245161061 1102 66-67 0.1035368782035313 1102 68-69 0.0750030968214439 1102 70-71 0.02143006841937023 1102 72-73 0.12169179955697373 1102 74-75 -0.12993251587780819 1102 76-77 -0.006941163368832548 1102 78-79 0.009006476776164618 1102 80-81 -0.040345867368667854 1102 82-83 0.044710283120384275 1102 84-85 0.08908474984140113 1102 86-87 0.011308420410816922 1102 88-89 0.13572498534417576 1102 90-91 0.029592152875231648 1102 92-93 -0.06278149653792298 1102 94-95 -0.2054442584831797 1102 96-97 -0.017135466409751388 1102 98-99 -0.1883531456864347 1102 100-101 0.27971554938319443 1103 1 0.06539203473053234 1103 2 0.016270178594886886 1103 3 -0.019394195423451777 1103 4 0.017289588680142742 1103 5 -0.15809633269469714 1103 6 -0.21432314660384577 1103 7 -0.1197192096852362 1103 8 -0.0607018768529457 1103 9 -0.15043098475442207 1103 10-11 -0.24517184631883993 1103 12-13 -0.10529793824548506 1103 14-15 -0.2072344751009112 1103 16-17 -0.21293291511057788 1103 18-19 -0.22007693883796975 1103 20-21 -0.13344152345532478 1103 22-23 -0.023284688249241015 1103 24-25 -0.060439722251551586 1103 26-27 -0.08803749511083936 1103 28-29 -0.19685892737980026 1103 30-31 -0.14131605002791758 1103 32-33 -0.21481152383841362 1103 34-35 -0.06987349154486111 1103 36-37 -0.17661632454910858 1103 38-39 -0.2751795271827149 1103 40-41 -0.1407620811276047 1103 42-43 -0.03622532636670428 1103 44-45 0.07493960625018303 1103 46-47 0.10324420090979203 1103 48-49 0.2942875858171945 1103 50-51 0.16140748531436344 1103 52-53 0.13772760463658784 1103 54-55 0.11613746838492744 1103 56-57 -0.07896204138987883 1103 58-59 0.11002369951351199 1103 60-61 -0.10033659518495597 1103 62-63 0.078784467988676 1103 64-65 -0.09956932467910207 1103 66-67 -0.12657518221833897 1103 68-69 0.0703082593181783 1103 70-71 0.0836091034505273 1103 72-73 0.19375410029132212 1103 74-75 0.15042855260726995 1103 76-77 0.07224607928175075 1103 78-79 0.08819274893776452 1103 80-81 0.14418251962817763 1103 82-83 -0.0753324106309492 1103 84-85 -0.048472746778383424 1103 86-87 0.2121216125392955 1103 88-89 0.07966992989237909 1103 90-91 -0.0804333476932797 1103 92-93 0.0054172875558400335 1103 94-95 0.25122185565176025 1103 96-97 0.25176470948167307 1103 98-99 0.2706933838255523 1103 100-101 0.4464775827672369 1104 1 0.13018870658096304 1104 2 0.09699572483823715 1104 3 0.0758504070812478 1104 4 0.01953965088907239 1104 5 0.024118802164096564 1104 6 -0.03380506385109783 1104 7 -0.07167245717965898 1104 8 0.09141317032984375 1104 9 0.032836713921710725 1104 10-11 0.05277956935314165 1104 12-13 0.09659987647994228 1104 14-15 0.1366339077416967 1104 16-17 0.07097509322108664 1104 18-19 -0.042449642048474345 1104 20-21 -0.1631788982422009 1104 22-23 -0.07458475138049181 1104 24-25 -0.033498643614564116 1104 26-27 -0.10556177918110166 1104 28-29 -0.1671249406738795 1104 30-31 0.08737489713269753 1104 32-33 0.09694801577409606 1104 34-35 0.22673078549325254 1104 36-37 -0.02599673865542229 1104 38-39 -0.15998684513642303 1104 40-41 -0.006922530853941566 1104 42-43 -0.017021495275329812 1104 44-45 0.032034078370230645 1104 46-47 0.00446338301951954 1104 48-49 0.16283211517698248 1104 50-51 0.05047625620517948 1104 52-53 0.31860934141206343 1104 54-55 0.3025045082298874 1104 56-57 0.3479444411759971 1104 58-59 0.1959267981394177 1104 60-61 0.22123670914640314 1104 62-63 0.22786699448818126 1104 64-65 0.11905996317263856 1104 66-67 0.20600147970124993 1104 68-69 0.2269646525704765 1104 70-71 0.11869042304029875 1104 72-73 0.2061149489830143 1104 74-75 0.1521321926485122 1104 76-77 0.350393220626092 1104 78-79 0.4941270103794082 1104 80-81 0.48488944648440224 1104 82-83 0.46479037342293594 1104 84-85 0.47070228562484573 1104 86-87 0.5756369121717633 1104 88-89 0.6103130516551118 1104 90-91 0.4765750671704545 1104 92-93 0.7035180046619978 1104 94-95 0.7492021829479079 1104 96-97 0.7114079596150304 1104 98-99 0.8300056937400946 1104 100-101 1.1887187832621997 1105 1 -0.08535608555531837 1105 2 -0.09157051835595098 1105 3 -0.13296737645072199 1105 4 0.0031575469360234365 1105 5 -0.06321208820700974 1105 6 -0.18997624075007735 1105 7 -0.08544321176213998 1105 8 -0.05789806074587034 1105 9 0.0017452030227786963 1105 10-11 -0.11050702402465618 1105 12-13 -0.23768193501122425 1105 14-15 -0.22882592017204217 1105 16-17 -0.19497525376431923 1105 18-19 -0.12468595433148977 1105 20-21 -0.10778010585160303 1105 22-23 -0.23512956278303676 1105 24-25 -0.2082137919069993 1105 26-27 -0.13079846522963123 1105 28-29 -0.2149295499347872 1105 30-31 -0.1456357262850574 1105 32-33 -0.22104975704583296 1105 34-35 -0.21552032328022364 1105 36-37 -0.22386839451372254 1105 38-39 -0.3009729310408318 1105 40-41 -0.19928133804103254 1105 42-43 -0.3429966572082819 1105 44-45 -0.12217829750687059 1105 46-47 -0.26465896160769375 1105 48-49 -0.3587524579404118 1105 50-51 -0.3101908959713242 1105 52-53 -0.14364747017544488 1105 54-55 -0.22320612790360883 1105 56-57 -0.14625679880072795 1105 58-59 -0.3168721661062577 1105 60-61 -0.34217742689654074 1105 62-63 -0.37498922909295374 1105 64-65 -0.39574885769349777 1105 66-67 -0.3966904026512381 1105 68-69 -0.5748421376314745 1105 70-71 -0.35562668424581645 1105 72-73 -0.22345722436711668 1105 74-75 -0.19073312460905 1105 76-77 -0.22754286161773507 1105 78-79 -0.3936730135107567 1105 80-81 -0.34523836590842905 1105 82-83 -0.3905629505855295 1105 84-85 -0.28069047257198676 1105 86-87 -0.22950756553909812 1105 88-89 -0.20827889273474298 1105 90-91 -0.23652849670298437 1105 92-93 -0.02809196139084591 1105 94-95 -0.03489659903890541 1105 96-97 0.03532548459374141 1105 98-99 -0.09650545564666047 1105 100-101 0.12684016842948864 1106 1 -0.16228603012766385 1106 2 -0.14484761115284073 1106 3 -0.148065521835683 1106 4 -0.022231293960572884 1106 5 -0.17188335386707365 1106 6 -0.12815890813869402 1106 7 -0.1576082795277145 1106 8 -0.13114522839695297 1106 9 -0.13093685883630002 1106 10-11 -0.36330382649803283 1106 12-13 -0.43888493413909657 1106 14-15 -0.29491543934437914 1106 16-17 -0.23658773965940583 1106 18-19 -0.1436977218577411 1106 20-21 -0.18014625902371506 1106 22-23 -0.07894117489693286 1106 24-25 -0.18787275271083814 1106 26-27 -0.11069550454151766 1106 28-29 -0.09317016629358932 1106 30-31 -0.1968802651526076 1106 32-33 -0.21523956678969114 1106 34-35 -0.34235230846645237 1106 36-37 -0.2659188043311431 1106 38-39 -0.09308844045031606 1106 40-41 -0.22143565920728037 1106 42-43 -0.49620472377106495 1106 44-45 -0.48081319006154644 1106 46-47 -0.0854561112788943 1106 48-49 0.23415623316512324 1106 50-51 0.2602898490160044 1106 52-53 0.28368257855625245 1106 54-55 0.11884221526010919 1106 56-57 0.26442532092465143 1106 58-59 0.11889912972696237 1106 60-61 0.19024258327139876 1106 62-63 0.16234673026836077 1106 64-65 0.07983114867179353 1106 66-67 0.12073107484083678 1106 68-69 0.31669119542630497 1106 70-71 0.38740249277134 1106 72-73 0.41308451997128515 1106 74-75 0.3198574423567315 1106 76-77 0.31886322757077323 1106 78-79 0.3431004027091653 1106 80-81 0.3449070025139278 1106 82-83 0.10138350655001105 1106 84-85 0.18535399354935933 1106 86-87 0.32347234245348133 1106 88-89 0.34840983201591413 1106 90-91 -0.03493076671542994 1106 92-93 0.12671920845915352 1106 94-95 -0.29053050373310185 1106 96-97 0.2919819695464341 1106 98-99 0.32468353156902197 1106 100-101 0.6547693400733436 1107 1 0.07563589145534522 1107 2 0.027711145160118633 1107 3 0.08341413094014172 1107 4 0.019695179532078555 1107 5 0.030911744365766936 1107 6 0.05421843012273797 1107 7 0.07879006899432994 1107 8 0.0362691176422274 1107 9 -0.03513044666276244 1107 10-11 -0.04638652265806087 1107 12-13 -0.08659628287745136 1107 14-15 -0.05424262195683838 1107 16-17 -0.09078555963326096 1107 18-19 -0.0866060535452462 1107 20-21 -0.007183097515564896 1107 22-23 -0.03624351010181215 1107 24-25 -4.7796859738724606E-4 1107 26-27 8.827102446673507E-4 1107 28-29 -0.07875265084624772 1107 30-31 -0.24881341603337148 1107 32-33 -0.10537302050411057 1107 34-35 0.01953861498811449 1107 36-37 -0.11558352421001672 1107 38-39 -0.08959927004524815 1107 40-41 -0.022035683659694882 1107 42-43 0.09951234306865331 1107 44-45 0.09520558186333261 1107 46-47 -0.06346775438660046 1107 48-49 -0.009403849220674942 1107 50-51 0.024991858815774037 1107 52-53 -0.10933343777043092 1107 54-55 -0.1055180251258605 1107 56-57 0.0737388666177381 1107 58-59 -0.04865628789193721 1107 60-61 0.09773724606330347 1107 62-63 -0.16903981526484557 1107 64-65 -0.12612865533275652 1107 66-67 0.0740222747956949 1107 68-69 0.05117097046415964 1107 70-71 -0.04800083087507545 1107 72-73 -0.20768215704062953 1107 74-75 -0.15303330240716306 1107 76-77 -0.1906308897443516 1107 78-79 -0.2698273968563356 1107 80-81 -0.13464232081272698 1107 82-83 -0.19244508207586009 1107 84-85 -0.12312722982337831 1107 86-87 -0.21069989654058574 1107 88-89 -0.21599404651205134 1107 90-91 0.01052719865620233 1107 92-93 0.05607056133560562 1107 94-95 -0.0986073219106487 1107 96-97 -0.12456478627746748 1107 98-99 0.13820571272373883 1107 100-101 0.6697934633134786 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2316 70-71 -0.4281496599988728 2316 72-73 -0.7354594163842236 2316 74-75 -0.7134910235067515 2316 76-77 -0.6335228459861391 2316 78-79 -0.7232189784923904 2316 80-81 -0.7294091276343728 2316 82-83 -0.7587818468510115 2316 84-85 -0.49939269064175207 2316 86-87 -0.8172807816055006 2316 88-89 -0.6428561658295315 2316 90-91 -0.4467775242199892 2316 92-93 -0.35873663002793066 2316 94-95 -0.7703336099900078 2316 96-97 -0.5808127921574027 2316 98-99 -0.9605213387869966 2316 100-101 -0.8228970590282216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 11.0 5 49.0 6 126.0 7 264.0 8 583.0 9 1093.0 10 1908.0 11 3271.0 12 5044.0 13 6772.0 14 8836.0 15 12953.0 16 18391.0 17 25818.0 18 22085.0 19 11754.0 20 8979.0 21 9033.0 22 9189.0 23 9827.0 24 10778.0 25 12611.0 26 14979.0 27 17658.0 28 21730.0 29 26818.0 30 32680.0 31 40333.0 32 50501.0 33 65032.0 34 88855.0 35 126558.0 36 179540.0 37 259827.0 38 207745.0 39 18850.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.374432705634106 20.05712235961368 16.528711204961432 19.039733729790782 2 15.825170914732038 22.424929800193613 39.104223412762835 22.645675872311514 3 19.260451804150055 27.669847454467835 31.427641587146972 21.642059154235138 4 12.657010059466902 18.540565566919806 37.502405132552866 31.300019241060422 5 12.321413402273429 39.35772347333595 32.81700929984657 15.503853824544048 6 28.598980223797582 38.15276800692678 16.984294484429775 16.263957284845862 7 25.925449910108174 36.70239791715521 20.165458087558854 17.20669408517777 8 27.448424938819443 32.82772937448515 19.31013282344523 20.413712863250176 9 27.871502786947346 16.358584218722754 20.487069406112646 35.282843588217254 10-11 25.00676443530494 27.98258984673293 25.423867032246818 21.586778685715316 12-13 27.396858205870195 26.113908827840206 26.934608591078206 19.554624375211393 14-15 23.72625683207966 26.02627757634793 28.14850641268467 22.098959178887746 16-17 20.196612070157716 29.71887006872666 28.277068263674682 21.80744959744094 18-19 20.228818298248463 26.42902453836487 33.567608276061115 19.77454888732555 20-21 21.982686052035042 24.534193468862554 34.03848813367727 19.444632345425138 22-23 21.389071468272714 24.98369938777343 34.16258822530208 19.46464091865177 24-25 21.048054692878683 25.210449731075308 34.271964187468626 19.46953138857739 26-27 20.357490613268936 25.372866775876318 34.49897536093489 19.77066724991986 28-29 20.13439944920353 26.146108383937005 34.09477487472161 19.62471729213785 30-31 20.261137266927623 25.51947105122331 34.11398674771963 20.10540493412943 32-33 20.11122986453091 25.764493930046722 33.851489077691795 20.272787127730577 34-35 20.85798594200023 25.643861500441943 33.72875215710326 19.769400400454572 36-37 20.915446061896088 25.27677814456049 33.7851224513111 20.022653342232324 38-39 20.446482794283 25.859609406473417 33.56982550763329 20.124082291610296 40-41 20.420439718358978 26.038679041073653 33.34235258040658 20.198528660160783 42-43 20.485929841454904 26.192696394201224 32.56565829684763 20.755715467496245 44-45 20.75700795497592 26.085278484285467 32.41179176362357 20.745921797115045 46-47 21.218041801203768 26.790809086590784 31.51926210533278 20.471887006872667 48-49 21.308685234289975 26.98739109259159 30.771604103156136 20.9323195699623 50-51 21.0533653817246 27.519752151090426 30.54011009494261 20.886772372242365 52-53 21.57524156550078 27.855794264961425 29.458739951055552 21.11022421848224 54-55 20.911086759144013 27.74617283282525 29.72420645657834 21.6185339514524 56-57 21.440178340578793 27.661504650925075 29.541980085502463 21.35633692299367 58-59 21.872500916956785 27.271196733829996 29.48673719717878 21.369565152034443 60-61 21.429543145071584 27.48630577652711 30.377650907035616 20.706500171365693 62-63 21.371406581645232 27.638731052065108 30.225300791889893 20.76456157439977 64-65 21.14385849402625 27.84500874866966 29.975242166783918 21.03589059052017 66-67 21.443598138427404 27.624676058709284 29.50146863406509 21.430257168798214 68-69 21.36960273223058 27.626517488320072 29.75885539741809 21.245024382031254 70-71 21.73198856359471 27.790968426622413 29.345360499305517 21.131682510477358 72-73 21.47915985524076 27.825529788009636 29.48158002532911 21.213730331420496 74-75 20.936829193498927 28.64035601974614 29.108755584417146 21.31405920233779 76-77 21.108495529459866 27.843731022000952 29.56340079730144 21.48437265123774 78-79 21.059603694283602 28.096908803386427 29.313567653372296 21.529919848957675 80-81 21.266369933437957 28.298526555669802 29.04471893019704 21.390384580695205 82-83 21.150203760129756 28.053696168167118 29.325148855380668 21.470951216322458 84-85 20.868920788309964 28.24667137428864 29.06668450502136 21.817723332380034 86-87 20.90278153579739 28.184094858427883 28.757004948560226 22.156118657214495 88-89 20.77237825519659 27.94050002705774 28.6726749884253 22.61444672932037 90-91 20.870866920632245 27.82343779597163 28.871421656027973 22.43427362736815 92-93 21.11544666683954 27.751289674374835 29.077887049816546 22.055376608969077 94-95 20.62051668260067 27.63914443422263 29.06411031140454 22.67622857177216 96-97 20.438966755055286 27.729524805935867 29.330065359477125 22.50144307953172 98-99 20.45574289714969 27.953072822207208 28.788030247571 22.8031540330721 100-101 19.95306443604973 27.74606687265496 29.345175968901057 22.955692722394254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2288.0 1 1582.0 2 1129.5 3 1350.0 4 1078.0 5 1029.0 6 1930.0 7 4101.5 8 6681.5 9 7984.0 10 6712.0 11 4874.0 12 4577.5 13 4742.5 14 4831.5 15 4973.5 16 5061.5 17 5122.5 18 5234.5 19 5353.5 20 5602.5 21 5916.5 22 6441.0 23 7198.5 24 7993.5 25 8913.0 26 10123.0 27 11786.5 28 13395.0 29 15355.0 30 17615.0 31 19512.0 32 22114.0 33 24020.0 34 25606.5 35 27128.5 36 29227.5 37 32345.0 38 34622.0 39 35598.5 40 36646.5 41 39095.5 42 40908.5 43 42664.5 44 45404.0 45 47397.0 46 46772.0 47 50043.0 48 57989.5 49 55822.5 50 46826.0 51 41034.0 52 37184.5 53 35971.0 54 35612.0 55 33113.5 56 30013.0 57 27378.0 58 25388.5 59 23327.5 60 19812.5 61 16834.0 62 14509.0 63 10812.5 64 7429.5 65 5535.0 66 4030.0 67 3147.5 68 2518.0 69 1828.5 70 1244.0 71 927.5 72 812.5 73 716.5 74 556.5 75 428.5 76 294.0 77 170.5 78 114.0 79 80.5 80 41.5 81 21.0 82 13.0 83 8.0 84 4.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05343903890903187 2 0.0 3 0.0 4 0.0 5 0.018865258461556962 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0028560949065305364 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012401464725724696 24-25 3.006415691084775E-4 26-27 0.0014656276494038278 28-29 0.0038331800061330882 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01296516766780309 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.2548117683135813E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5032078455423875E-4 84-85 0.0016159484339580664 86-87 0.0 88-89 0.0 90-91 0.009319888642362802 92-93 0.0012401464725724696 94-95 0.0 96-97 0.0 98-99 7.516039227711938E-5 100-101 0.011537120214537825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1330488.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.456466985015496 #Duplication Level Percentage of deduplicated Percentage of total 1 69.05905035598997 29.320032914556105 2 15.118096310919467 12.837219138016739 3 6.024169345337842 7.6729484076743555 4 3.032563405163405 5.150077123651451 5 1.7679463005114897 3.753037686947317 6 1.1482974091077272 2.925159062325663 7 0.7275752064331883 2.162319091173254 8 0.5182135913779663 1.760121458681995 9 0.38564421740970706 1.4735781885975627 >10 1.9943513661495134 15.3230882643663 >50 0.14125148853042044 4.1441483418965355 >100 0.07294955118561754 5.625391214528315 >500 0.005828891288592909 1.5985328990533472 >1k 0.0033560283176747056 2.4460943001183186 >5k 1.7663306935130027E-4 0.40698245651916476 >10k+ 5.298992080539008E-4 3.401269451893627 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19240 1.4460859474117767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16003 1.2027917576107412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10112 0.760021886706231 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5427 0.4078954488879269 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3628 0.27268190318138913 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2888 0.21706321289632075 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2814 0.2115013438678139 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2752 0.2068413995466325 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2147 0.1613693622189753 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1633 0.12273692058853594 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1570 0.11800181587507741 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1520 0.11424379626122144 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1481 0.1113125409624138 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1426 0.10717871938717223 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.10304489781193067 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1348 0.10131620878955691 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 0.10109072761272556 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.758019613855969E-5 0.0 14-15 0.0 0.0 0.0 7.516039227711938E-5 0.0 16-17 0.0 0.0 0.0 7.516039227711938E-5 0.0 18-19 0.0 0.0 0.0 7.516039227711938E-5 0.0 20-21 0.0 0.0 0.0 1.1274058841567906E-4 0.0 22-23 0.0 0.0 0.0 2.2548117683135813E-4 0.0 24-25 0.0 0.0 0.0 3.758019613855968E-4 0.0 26-27 0.0 0.0 0.0 9.770850996025518E-4 0.0 28-29 0.0 0.0 0.0 0.0022172315721750214 0.0 30-31 0.0 0.0 0.0 0.005449128440091155 0.0 32-33 0.0 0.0 0.0 0.009131987661670005 0.0 34-35 0.0 0.0 0.0 0.011912922175923421 0.0 36-37 0.0 0.0 0.0 0.01439321512106836 0.0 38-39 0.0 0.0 0.0 0.01676076747779762 0.0 40-41 0.0 0.0 0.0 0.02044362669937647 0.0 42-43 0.0 0.0 0.0 0.023525202782738362 0.0 44-45 0.0 0.0 0.0 0.027057741219762975 0.0 46-47 0.0 0.0 0.0 0.03235654887529989 0.0 48-49 0.0 0.0 0.0 0.040361130652813104 0.0 50-51 0.0 0.0 0.0 0.05411548243952595 0.0 52-53 0.0 0.0 0.0 0.08647203131482584 0.0 54-55 0.0 0.0 0.0 0.12097065137002364 0.0 56-57 0.0 0.0 0.0 0.1648267402637228 0.0 58-59 0.0 0.0 0.0 0.23085514487917214 0.0 60-61 0.0 0.0 0.0 0.3378459632856516 0.0 62-63 0.0 0.0 0.0 0.4128560347782167 0.0 64-65 0.0 0.0 0.0 0.4901209180390954 7.516039227711938E-5 66-67 0.0 0.0 0.0 0.6219522460931628 7.516039227711938E-5 68-69 7.516039227711938E-5 0.0 0.0 0.7985415877482548 7.516039227711938E-5 70-71 7.516039227711938E-5 0.0 0.0 0.9505159009325902 7.516039227711938E-5 72-73 7.516039227711938E-5 0.0 0.0 1.0928321037093156 7.516039227711938E-5 74-75 7.516039227711938E-5 0.0 0.0 1.3345855054686702 7.516039227711938E-5 76-77 7.516039227711938E-5 0.0 0.0 1.525192260283445 7.516039227711938E-5 78-79 7.516039227711938E-5 0.0 0.0 1.6879896699556856 7.516039227711938E-5 80-81 7.516039227711938E-5 0.0 0.0 1.894041885383408 7.516039227711938E-5 82-83 7.516039227711938E-5 0.0 0.0 2.247032667712899 1.1274058841567906E-4 84-85 7.516039227711938E-5 0.0 0.0 2.601413917299517 1.5032078455423875E-4 86-87 7.516039227711938E-5 0.0 0.0 2.9748859065245234 1.5032078455423875E-4 88-89 7.516039227711938E-5 0.0 0.0 3.475566859678554 1.5032078455423875E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14030 0.0 30.287413 1 GTATCAA 19830 0.0 28.4039 1 GAGTACT 13275 0.0 25.333982 12-13 CCCTATA 175 1.4834404E-7 24.433792 2 GTACTTT 13850 0.0 24.074623 14-15 TAACGTG 100 0.0016517267 23.755074 5 TATCAAC 24035 0.0 23.40421 2 ATCAACG 24075 0.0 23.38506 3 TCAACGC 24190 0.0 23.254244 4 CAACGCA 24390 0.0 23.083036 5 AGTACTT 13370 0.0 22.79135 12-13 GTACATG 11295 0.0 22.766348 1 AACGCAG 24795 0.0 22.738338 6 TACATGG 11560 0.0 21.946732 2 ACTTTTT 15505 0.0 21.64316 16-17 ATGGGAG 2575 0.0 21.587135 5 AGAGTAC 20035 0.0 21.433046 10-11 TAAGGTG 500 0.0 20.904467 5 GGACCGA 365 0.0 20.820887 6 TACTTTT 14585 0.0 20.695755 14-15 >>END_MODULE