FastQCFastQC Report
Thu 26 May 2016
SRR937516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438056
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170221.1836813030925082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127540.8868917483046558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78420.5453195146781488No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51150.35568851282564795No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34680.24115889784542466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29810.2072937354317217No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.2004789799562743No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25350.1762796441863182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21820.15173261681047193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.1465172427221193No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17500.12169206206156089No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16050.11160900549074584No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15220.10583732483296895No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15090.10493332665765451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188800.031.1844521
GGTATCA127750.026.8190351
GTACATG132350.025.8150961
TATCAAC228050.025.791022
ATCAACG228950.025.7302383
TCAACGC229650.025.6724974
CAACGCA230700.025.5135785
TACATGG134300.025.2934442
AACGCAG235150.025.0096826
GAGTACT112200.024.9574812-13
AGTACTT113400.024.0650512-13
ACATGGG135400.023.8239573
GTACTTT118250.023.79985414-15
CGACCGT701.7828159E-523.74633218-19
ACGCAGA256850.022.6008197
AGAGTAC186350.022.58468810-11
CGCAGAG258200.022.4626858
CATGGGG41000.021.899834
GCAGAGT261100.021.8493429
TACTTTT124800.021.75147414-15
Produced by FastQC (version 0.11.5)