##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937516_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1438056 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63926022352398 33.0 31.0 34.0 30.0 34.0 2 31.678172477288783 33.0 31.0 34.0 30.0 34.0 3 31.685910701669474 33.0 31.0 34.0 30.0 34.0 4 35.35189172048933 37.0 35.0 37.0 33.0 37.0 5 35.38578678438114 37.0 35.0 37.0 33.0 37.0 6 35.44210030763753 37.0 35.0 37.0 33.0 37.0 7 35.34101523167387 37.0 35.0 37.0 33.0 37.0 8 35.34689469672947 37.0 35.0 37.0 33.0 37.0 9 36.918005279349345 39.0 37.0 39.0 33.0 39.0 10-11 36.80741883487153 39.0 37.0 39.0 33.0 39.0 12-13 36.6402236769639 39.0 37.0 39.0 32.0 39.0 14-15 37.67474076113865 40.0 37.0 41.0 32.0 41.0 16-17 37.647499123817155 40.0 37.0 41.0 32.0 41.0 18-19 37.71606147465745 40.0 37.0 41.0 32.0 41.0 20-21 37.66025592883726 40.0 37.0 41.0 32.0 41.0 22-23 37.510200228642 40.0 37.0 41.0 32.0 41.0 24-25 37.414588861629866 40.0 37.0 41.0 32.0 41.0 26-27 37.396693870057916 40.0 37.0 41.0 32.0 41.0 28-29 37.296394577123564 40.0 37.0 41.0 32.0 41.0 30-31 36.99421302091156 39.0 36.0 41.0 31.0 41.0 32-33 36.6583457111545 39.0 35.0 41.0 30.5 41.0 34-35 36.57768960318653 39.0 35.0 41.0 30.0 41.0 36-37 36.37352022452533 39.0 35.0 41.0 30.0 41.0 38-39 36.20890076603415 39.0 35.0 41.0 30.0 41.0 40-41 35.928779894524276 39.0 35.0 41.0 29.5 41.0 42-43 35.552450669514954 38.5 35.0 40.0 27.5 41.0 44-45 35.148213977758864 38.0 35.0 40.0 25.5 41.0 46-47 34.574616357082064 38.0 34.0 40.0 23.5 41.0 48-49 34.06376142514617 38.0 34.0 40.0 20.0 41.0 50-51 32.99341124406838 37.5 32.0 39.5 16.5 40.5 52-53 33.08067314485667 37.5 33.0 39.5 15.0 40.5 54-55 33.41163835066229 38.0 33.0 40.0 13.5 41.0 56-57 33.170829925955594 38.0 33.0 40.0 9.5 41.0 58-59 32.88793899542159 37.0 33.0 40.0 8.0 41.0 60-61 32.550272729295656 37.0 32.0 40.0 6.5 41.0 62-63 32.237241108830254 36.0 32.0 39.5 2.0 41.0 64-65 31.807439696367876 36.0 31.0 39.0 2.0 41.0 66-67 31.433081882763954 35.0 31.0 39.0 2.0 40.5 68-69 30.950169882118637 35.0 30.5 38.5 2.0 40.0 70-71 30.466998851226933 35.0 30.0 38.0 2.0 40.0 72-73 30.01299636453657 35.0 29.5 37.0 2.0 39.5 74-75 29.544021234221756 34.5 29.0 36.5 2.0 39.0 76-77 29.077931248852618 34.0 29.0 36.0 2.0 39.0 78-79 28.581120276261842 34.0 28.0 35.5 2.0 37.5 80-81 28.089213146080542 34.0 27.0 35.0 2.0 37.0 82-83 27.61351957086511 34.0 26.0 35.0 2.0 36.5 84-85 27.088767405441793 33.0 25.5 35.0 2.0 36.0 86-87 26.650651295916152 33.0 25.0 35.0 2.0 36.0 88-89 26.17958549597512 33.0 24.0 35.0 2.0 35.0 90-91 25.65799558570737 32.0 21.5 35.0 2.0 35.0 92-93 25.144685951033896 32.0 19.5 35.0 2.0 35.0 94-95 24.607139777588635 32.0 14.0 34.0 2.0 35.0 96-97 23.87488317562042 31.0 2.0 34.0 2.0 35.0 98-99 22.97702905867365 31.0 2.0 34.0 2.0 35.0 100-101 20.5624645354562 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09980856791117887 1101 2 0.19819719170091687 1101 3 0.09131769042469529 1101 4 0.05198242924210206 1101 5 0.02577056859181681 1101 6 0.08622451067842718 1101 7 -0.043912157227488535 1101 8 -0.030996976688058453 1101 9 0.09539547616785882 1101 10-11 -0.11266301299725257 1101 12-13 -0.06866578511997545 1101 14-15 0.02630081098107695 1101 16-17 -0.1363256530167689 1101 18-19 0.03673791426583506 1101 20-21 0.10003954433144457 1101 22-23 0.073042984276249 1101 24-25 0.09549811829828769 1101 26-27 0.057950024798067545 1101 28-29 -0.0550308976951257 1101 30-31 0.06322906760745894 1101 32-33 -0.23605551863100516 1101 34-35 0.07579415578216242 1101 36-37 0.06387172328668811 1101 38-39 0.07490667106862503 1101 40-41 0.07272816689788186 1101 42-43 0.2795886628769608 1101 44-45 0.29451436446665724 1101 46-47 0.3120901862223562 1101 48-49 0.3643588940755009 1101 50-51 0.1785957423937603 1101 52-53 0.11433291472729223 1101 54-55 0.050551392843999565 1101 56-57 0.14579516285641603 1101 58-59 0.11567331803777847 1101 60-61 0.17990484179490096 1101 62-63 0.2960943517223953 1101 64-65 0.2781513626450618 1101 66-67 0.13594048340073428 1101 68-69 0.10466344631393554 1101 70-71 0.11331007399847337 1101 72-73 0.08423874750331706 1101 74-75 0.16888809338991706 1101 76-77 0.27404354015546417 1101 78-79 0.1540124032532475 1101 80-81 0.15517047756202018 1101 82-83 0.25988172647425856 1101 84-85 0.24450568801885098 1101 86-87 0.36068476672874894 1101 88-89 0.3584333104565651 1101 90-91 0.22477114667619702 1101 92-93 0.5327479627313991 1101 94-95 0.3917792249930159 1101 96-97 0.5604285457198799 1101 98-99 0.45027449738302394 1101 100-101 0.5839567609602376 1102 1 -0.029416672102023256 1102 2 -0.060647590512839855 1102 3 0.09238011576284677 1102 4 0.008107548628046857 1102 5 0.02730396598708751 1102 6 -0.15815668315176623 1102 7 -0.05244952977295725 1102 8 -0.06673900959330581 1102 9 0.080354308098741 1102 10-11 0.06423518694024466 1102 12-13 0.17742551190055877 1102 14-15 0.2961758317914729 1102 16-17 0.2790435890760179 1102 18-19 0.1954529418523805 1102 20-21 0.1474182379936977 1102 22-23 0.01838211336494311 1102 24-25 0.27270299771483764 1102 26-27 0.1789551594887726 1102 28-29 0.2377574613359954 1102 30-31 0.21881852016998238 1102 32-33 0.0755782943879808 1102 34-35 0.21170573862798392 1102 36-37 0.08969815146381421 1102 38-39 0.3342198696771703 1102 40-41 0.30616491936665824 1102 42-43 0.2220811492297159 1102 44-45 0.36204701122738925 1102 46-47 0.3745819715934573 1102 48-49 0.4848664048019842 1102 50-51 0.4610457171292168 1102 52-53 0.3877457023853452 1102 54-55 0.538076474844253 1102 56-57 0.5936906844597942 1102 58-59 0.34235508988577124 1102 60-61 0.66773149194956 1102 62-63 0.6416250094476439 1102 64-65 0.4949839769853952 1102 66-67 0.5082687209867878 1102 68-69 0.5271450966584403 1102 70-71 0.563734240925573 1102 72-73 0.6993107295581815 1102 74-75 0.48863357862986945 1102 76-77 0.434723506975665 1102 78-79 0.2928352505823639 1102 80-81 0.48953800224971644 1102 82-83 0.4327646158038583 1102 84-85 0.3924420272005129 1102 86-87 0.3836718540623032 1102 88-89 0.35150381460842084 1102 90-91 0.45368620230201273 1102 92-93 0.5692577896181348 1102 94-95 0.438303962343948 1102 96-97 0.8967814549398341 1102 98-99 0.6509914701936985 1102 100-101 0.7437342138849381 1103 1 -0.06887482824583913 1103 2 -0.10023291616852603 1103 3 -0.1081662044601579 1103 4 -0.03346670819333042 1103 5 -0.21455209847259482 1103 6 -0.19011183809509902 1103 7 -0.1990912373034277 1103 8 -0.08238085955362351 1103 9 -0.28287742527257365 1103 10-11 -0.07583694360689464 1103 12-13 -0.17294175364973086 1103 14-15 -0.25027664750533063 1103 16-17 -0.3450611375603998 1103 18-19 -0.45591692812950413 1103 20-21 -0.30083648762536797 1103 22-23 -0.1835198338546249 1103 24-25 -0.44491201439169714 1103 26-27 -0.3840471600831208 1103 28-29 -0.42882951243656464 1103 30-31 -0.4597071273771789 1103 32-33 -0.4399923406456949 1103 34-35 -0.2551589733585118 1103 36-37 -0.22667670356293712 1103 38-39 -0.2795434322537602 1103 40-41 -0.17257996841896528 1103 42-43 -0.26882877406163175 1103 44-45 -0.30807781836546866 1103 46-47 -0.047442770506769705 1103 48-49 -0.14220645824153877 1103 50-51 -0.24726048670824952 1103 52-53 -0.25365662125597765 1103 54-55 -0.2809943738342042 1103 56-57 -0.27621236150434925 1103 58-59 -0.21654219784287676 1103 60-61 -0.1168255512066878 1103 62-63 -0.32210624576629954 1103 64-65 -0.19870536315743692 1103 66-67 -0.10972678229927268 1103 68-69 0.06169450964397427 1103 70-71 0.035398330290135505 1103 72-73 -0.01407971136394437 1103 74-75 -0.12410473703538116 1103 76-77 -0.04563897700097996 1103 78-79 -0.2132027578413478 1103 80-81 -0.32192047944804614 1103 82-83 -0.1514648386898081 1103 84-85 -0.1474637656250941 1103 86-87 0.011665424371784638 1103 88-89 -0.10859547934645164 1103 90-91 -0.023662364243250522 1103 92-93 -0.1545783550092068 1103 94-95 -0.5831978587234588 1103 96-97 -0.2738291621883775 1103 98-99 0.32555705914113986 1103 100-101 0.6436187439681547 1104 1 -0.13090151795871563 1104 2 -0.07861616612147415 1104 3 -0.05293565657453314 1104 4 -0.16404236769964342 1104 5 -0.04527980940429188 1104 6 -0.12200626351059896 1104 7 -0.09982639924842118 1104 8 -0.23337878074206486 1104 9 -0.11372955473269997 1104 10-11 -0.0684872626893096 1104 12-13 -0.0942507352576385 1104 14-15 0.02189593262478695 1104 16-17 -0.008272164778098556 1104 18-19 0.012821771108264102 1104 20-21 -0.09947426334634457 1104 22-23 -0.1954548486121368 1104 24-25 -0.13369651233650615 1104 26-27 -0.04407793692703166 1104 28-29 0.009474229494735198 1104 30-31 -0.12937417211638547 1104 32-33 -0.06702612538683894 1104 34-35 0.18930295703901834 1104 36-37 0.08413628869209333 1104 38-39 -0.03997326478572205 1104 40-41 0.1966087522836446 1104 42-43 0.17390345682250086 1104 44-45 0.22068612408761368 1104 46-47 0.18920514761526164 1104 48-49 0.23849978785056436 1104 50-51 0.1748933425274899 1104 52-53 0.33639137680408027 1104 54-55 0.3640066095303709 1104 56-57 0.22657877256524728 1104 58-59 0.288992664369772 1104 60-61 0.17231694078717652 1104 62-63 0.38189890297552154 1104 64-65 0.4092106159176865 1104 66-67 0.3796886550441947 1104 68-69 0.36814104598017394 1104 70-71 0.5548805800309005 1104 72-73 0.4409669579203275 1104 74-75 0.49117431645331067 1104 76-77 0.40142182030678697 1104 78-79 0.3809559861025029 1104 80-81 0.5255089209674324 1104 82-83 0.625548785315285 1104 84-85 0.7276640893924302 1104 86-87 0.8147012012192256 1104 88-89 0.6362409754938518 1104 90-91 0.7695406480244884 1104 92-93 0.8212936144479812 1104 94-95 0.8595176302906964 1104 96-97 0.8219302807549766 1104 98-99 1.0450288760534576 1104 100-101 1.186779637215647 1105 1 -0.002454817618637861 1105 2 -0.03535286578771846 1105 3 -0.056004356114261356 1105 4 -0.10843276018789538 1105 5 -0.024059507993783313 1105 6 -0.08611286145526265 1105 7 -0.07541225751479175 1105 8 -0.09498098156964829 1105 9 -0.21272313404789145 1105 10-11 -0.2003670835317095 1105 12-13 -0.14040086844318722 1105 14-15 -0.20912822092158478 1105 16-17 -0.16916126941642062 1105 18-19 -0.05614472416595362 1105 20-21 -0.21597139404695298 1105 22-23 -0.06620487508423167 1105 24-25 -0.19169187387647213 1105 26-27 -0.2097143121898668 1105 28-29 -0.3284811763808264 1105 30-31 -0.29095602037699564 1105 32-33 -0.2972335978042935 1105 34-35 -0.17891864116005962 1105 36-37 -0.1435114702565059 1105 38-39 -0.1234022872711833 1105 40-41 -0.11149077864470058 1105 42-43 -0.11803812993512963 1105 44-45 -0.0549388578632275 1105 46-47 -0.22516795894280506 1105 48-49 -0.31947408660435883 1105 50-51 -0.2533293076792589 1105 52-53 -0.0977959163182689 1105 54-55 -0.11782992772788958 1105 56-57 -0.27401066115208295 1105 58-59 -0.09206617792671068 1105 60-61 0.15907333293713322 1105 62-63 0.09548620104849448 1105 64-65 0.06480495900421346 1105 66-67 -0.03436713024172633 1105 68-69 -0.049706608180205336 1105 70-71 -0.07843818792370882 1105 72-73 0.09653912633506323 1105 74-75 0.3151627732474225 1105 76-77 0.33833264443448385 1105 78-79 0.2906471427746382 1105 80-81 0.34284050691370993 1105 82-83 0.39599348890352815 1105 84-85 0.5906667324757855 1105 86-87 0.6763821307264557 1105 88-89 0.6269126583348523 1105 90-91 0.7267828958279914 1105 92-93 0.859278827207941 1105 94-95 0.9556996675001415 1105 96-97 1.0683568925613898 1105 98-99 1.2217204425272818 1105 100-101 1.4719722165047138 1106 1 -0.08504902408554216 1106 2 -0.07028842003266433 1106 3 -0.09606894501848373 1106 4 0.12547584193185912 1106 5 0.04795168054512544 1106 6 -0.03844848326661321 1106 7 -0.4223027713057803 1106 8 -0.3701380162060204 1106 9 -0.4666110654279052 1106 10-11 -0.8223015441170176 1106 12-13 -0.4785127595582068 1106 14-15 -0.5437544298162962 1106 16-17 -0.38406610385009543 1106 18-19 -0.48054621749806614 1106 20-21 -0.21842711800955072 1106 22-23 -0.3370624244850049 1106 24-25 -0.19919074299804862 1106 26-27 -0.6311483232867872 1106 28-29 -0.41647139070376227 1106 30-31 -0.47316630077758504 1106 32-33 -0.32570310416870996 1106 34-35 0.04630867971738439 1106 36-37 -0.29231860824657474 1106 38-39 -0.25155228469449753 1106 40-41 -0.4138218196827026 1106 42-43 -0.3647728974322533 1106 44-45 -0.20401797927090115 1106 46-47 -0.3696333826929674 1106 48-49 -0.17203831763566058 1106 50-51 -0.3464285157780722 1106 52-53 -0.0923791518330006 1106 54-55 -0.3204131975984268 1106 56-57 -0.23773022048168713 1106 58-59 -0.3098032186326094 1106 60-61 -0.3658804298818765 1106 62-63 -0.4513622268873547 1106 64-65 -0.6728913700785455 1106 66-67 -0.5427578186747013 1106 68-69 -0.5688621139834744 1106 70-71 -0.25545358504812654 1106 72-73 -0.5675363018240915 1106 74-75 -0.198183023606731 1106 76-77 -0.007664618553441471 1106 78-79 0.03750645004718933 1106 80-81 -0.21735872809713896 1106 82-83 -0.1593057501155002 1106 84-85 -0.3435547937233743 1106 86-87 -0.4831984135839349 1106 88-89 -0.29548331516537374 1106 90-91 -0.2615075022984712 1106 92-93 -0.11701638645650547 1106 94-95 0.02196413272348252 1106 96-97 0.09704326991404955 1106 98-99 0.02768460942775519 1106 100-101 0.37470164167491404 1107 1 -0.09531390744045964 1107 2 -0.1268323920633989 1107 3 -0.05868560461927075 1107 4 -0.04000946781498982 1107 5 -0.07219681906867237 1107 6 -0.15500064397374302 1107 7 -0.09273468042889732 1107 8 -0.026524312659333305 1107 9 0.0623440362336396 1107 10-11 -0.0926839436895861 1107 12-13 0.018417212739670674 1107 14-15 -0.024042804799606188 1107 16-17 -0.011220713029544527 1107 18-19 -0.16121614995600453 1107 20-21 -0.015262281695967772 1107 22-23 -3.1472283159672543E-4 1107 24-25 0.08863905449191378 1107 26-27 -0.0813425865672528 1107 28-29 -0.14606866462950308 1107 30-31 0.025929169897082716 1107 32-33 0.01708229443399034 1107 34-35 -0.17595508277614869 1107 36-37 -0.16537379760245585 1107 38-39 -0.02258150258878544 1107 40-41 -0.0918180912215334 1107 42-43 -0.04295065559334432 1107 44-45 0.04533955808630452 1107 46-47 0.07563460771292085 1107 48-49 0.12642949970458517 1107 50-51 -0.03951503698441883 1107 52-53 -0.15235612481036753 1107 54-55 -0.27606948226237904 1107 56-57 -0.09519933871804653 1107 58-59 -0.05351071446567346 1107 60-61 0.19384681750326394 1107 62-63 0.30003013847861126 1107 64-65 0.2855351263875505 1107 66-67 0.12136779329818737 1107 68-69 0.09046789548287038 1107 70-71 0.351075432484933 1107 72-73 0.3799066242629401 1107 74-75 0.4095147677736719 1107 76-77 0.33050376037900975 1107 78-79 0.5154812335699432 1107 80-81 0.5824513643013312 1107 82-83 0.5926543054048885 1107 84-85 0.33791803555143574 1107 86-87 0.6683509014129569 1107 88-89 0.5055666938363608 1107 90-91 0.7034568682478479 1107 92-93 0.616757257620641 1107 94-95 0.8541376455495069 1107 96-97 0.9569022039922999 1107 98-99 1.2744831854111247 1107 100-101 1.4034578339984698 1108 1 -0.1398182055967787 1108 2 -0.09630134353947639 1108 3 -0.0637034193461119 1108 4 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0.092070869870156 2316 22-23 0.14407929918380802 2316 24-25 0.2924420712114184 2316 26-27 0.27784366628633705 2316 28-29 0.34970524777101986 2316 30-31 0.34683455544381303 2316 32-33 0.07036588824897194 2316 34-35 -0.06114829515726683 2316 36-37 -0.07197240939391492 2316 38-39 0.11573101993955959 2316 40-41 -0.0931871217861513 2316 42-43 -0.3772725406733315 2316 44-45 -0.5987610177152405 2316 46-47 -0.30092042616048076 2316 48-49 -0.15584265201865577 2316 50-51 -0.3123842015638445 2316 52-53 -0.3553342572909344 2316 54-55 -0.43330004318365667 2316 56-57 -0.12126983974856387 2316 58-59 -0.2425755683895403 2316 60-61 -0.4398317965063612 2316 62-63 -0.6331392192669867 2316 64-65 -0.5148590619910607 2316 66-67 -0.4316119566024561 2316 68-69 -0.2711467980853435 2316 70-71 -0.034475922913092205 2316 72-73 -0.3268643582966497 2316 74-75 -0.18611981544028566 2316 76-77 -0.12474338140865626 2316 78-79 -0.07924528380114637 2316 80-81 -0.07791237036783727 2316 82-83 -0.25493735610143986 2316 84-85 -0.2895046286629146 2316 86-87 -0.27322557482944987 2316 88-89 -0.3205456290561379 2316 90-91 -0.36899777467201034 2316 92-93 0.13926019208278007 2316 94-95 0.1341731363671812 2316 96-97 -0.07208542967591569 2316 98-99 0.1573426916820928 2316 100-101 0.5484479908420035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5653.0 3 1231.0 4 1038.0 5 1084.0 6 1304.0 7 1593.0 8 2037.0 9 2698.0 10 3562.0 11 5182.0 12 7069.0 13 9762.0 14 11237.0 15 14981.0 16 19675.0 17 26407.0 18 21427.0 19 13324.0 20 12155.0 21 12433.0 22 13113.0 23 14586.0 24 16138.0 25 17876.0 26 20513.0 27 23795.0 28 28006.0 29 32969.0 30 39547.0 31 48197.0 32 59723.0 33 76869.0 34 102720.0 35 142485.0 36 191494.0 37 242920.0 38 174671.0 39 18580.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.145624636993006 19.366253405206105 15.80691967164993 19.681202286150963 2 15.153777361412871 21.70776062828349 40.03313725613203 23.10532475417161 3 19.066848339332946 27.783303073915455 30.632311482665013 22.51753710408659 4 12.158616959586046 17.607015010259612 38.08630341689714 32.148064613257205 5 12.49776086373157 39.29804384873545 32.63012256875107 15.57407271878191 6 28.700671483461814 37.49249627166669 17.70524309290958 16.101589151961914 7 26.383061204284342 36.16241565254496 20.380255548908625 17.07426759426207 8 27.251103225671518 32.372649763320624 19.915855078323794 20.460391932684065 9 28.261780330993197 16.26203964919545 20.621234396468253 34.8549456233431 10-11 24.829055538823635 27.730196376981336 25.803062499302836 21.637685584892193 12-13 27.10483760487717 25.861283439646975 27.020060912332468 20.013818043143388 14-15 23.662023767036203 25.877043786665478 27.89486488144364 22.566067564854677 16-17 20.4801469652683 29.785878994180297 27.71407307478462 22.019900965766784 18-19 20.523047173441448 26.922956272969103 32.49702342778431 20.05697312580514 20-21 22.392705923109123 25.12488294130214 32.54428713474811 19.938124000840627 22-23 21.63569695700254 25.625612298989058 32.71920173114851 20.01948901285989 24-25 21.221474550086967 25.783141935229807 32.86270530151779 20.132678213165434 26-27 20.587417286401475 26.023494739718352 33.013678196092485 20.37540977778769 28-29 20.34191943099877 26.75764950957666 32.6865815237738 20.21384953565077 30-31 20.443171851622555 26.11723703272999 32.68313972539325 20.756451390254206 32-33 20.315237526205305 26.480307763741965 32.393172642324345 20.81128206772838 34-35 21.077764735781038 26.332999943176095 32.22996181642671 20.35927350461616 36-37 21.0165272723344 26.03033194647648 32.34441076241811 20.60873001877101 38-39 20.566789597937376 26.58601901333889 32.14702682206518 20.700164566658557 40-41 20.631341600901916 26.71617682165018 31.99242362198962 20.660057955458285 42-43 20.589419227483784 26.803132054205577 31.275409080754567 21.332039637556072 44-45 20.742877998634217 26.77962450338675 31.205979333359128 21.271518164619906 46-47 21.242724944963197 27.38087560374895 30.47053635392282 20.90586309736503 48-49 21.271183870294106 27.585442321965893 29.7517742488226 21.391599558917402 50-51 20.993986089563688 28.097024421705857 29.606637962591737 21.30235152613872 52-53 21.51093840064357 28.273717884564892 28.733871015548473 21.481472699243064 54-55 20.874122509768483 28.13860510778104 29.120055445389916 21.867216937060558 56-57 21.37344987911993 28.154304093612087 28.74554824346643 21.726697783801548 58-59 21.806236475962493 27.674754084651326 28.82890981884323 21.690099620542945 60-61 21.37450461568909 27.914239541964196 29.6808286010354 21.030427241311305 62-63 21.335221976559218 28.099840231933754 29.551621462931895 21.013316328575137 64-65 21.03851640314732 28.329950055714328 29.28228521413604 21.349248327002318 66-67 21.234790728790646 28.194330234567726 28.883120322234458 21.687758714407167 68-69 21.178655168725022 28.03159889708406 29.21305560888786 21.576690325303055 70-71 21.54012871573475 28.226691615358078 28.798560223562575 21.434619445344595 72-73 21.23653150637006 28.271318123894286 28.925102613360803 21.56704775637485 74-75 20.759347048238197 29.06885655384092 28.63211735401488 21.539679043906002 76-77 20.93449266634045 28.435269876719794 28.850014428928116 21.78022302801164 78-79 20.985400696718397 28.5458338386563 28.774623168465617 21.694142296159686 80-81 21.148373045258527 28.73797098924075 28.406150629437644 21.707505336063075 82-83 21.08092286141398 28.461096185408586 28.702369876331552 21.75561107684588 84-85 20.900890290022893 28.660515816943633 28.42261961802101 22.015974275012468 86-87 20.971641690137705 28.61379044528408 28.162177721925886 22.252390142652327 88-89 20.918436865437016 28.371166224617916 27.97131858071859 22.739078329226476 90-91 20.828812753002925 28.241147184751604 28.34260103126737 22.5874390309781 92-93 21.331081271799132 28.12197373423467 28.496511964628652 22.05043302933755 94-95 20.929242256924347 27.897095612666583 28.56277657575218 22.610885554656893 96-97 20.624335441681446 27.858598369616928 28.901713153366902 22.615353035334724 98-99 20.71398025152108 28.157806595117847 28.356992216948168 22.77122093641291 100-101 20.863642746315918 27.40092885567615 28.998185703539008 22.73724269446892 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3569.0 1 2259.0 2 1123.0 3 1275.5 4 1129.0 5 1172.0 6 1970.5 7 3730.0 8 6135.5 9 7299.0 10 6021.5 11 4638.0 12 4605.0 13 4829.5 14 4882.0 15 4951.0 16 5005.0 17 5065.0 18 5263.5 19 5425.0 20 5583.5 21 6007.0 22 6635.5 23 7403.5 24 8314.0 25 9478.5 26 10839.5 27 12540.5 28 14342.0 29 16483.5 30 19060.5 31 21372.0 32 24261.5 33 26576.0 34 28094.0 35 29480.5 36 31831.0 37 35138.0 38 37419.5 39 38498.5 40 39546.0 41 42301.0 42 44529.5 43 46094.0 44 48640.5 45 50856.0 46 50881.5 47 54083.0 48 61764.5 49 60119.0 50 51201.5 51 44835.0 52 40846.5 53 39725.0 54 39075.5 55 36270.0 56 32929.0 57 29993.0 58 27900.0 59 25367.5 60 21662.0 61 18826.0 62 16204.0 63 12069.5 64 8263.0 65 6293.5 66 4742.0 67 3669.5 68 2855.5 69 2097.5 70 1517.0 71 1112.0 72 942.5 73 807.5 74 601.5 75 477.0 76 346.0 77 226.0 78 154.5 79 93.5 80 62.5 81 43.0 82 26.5 83 21.5 84 17.5 85 11.0 86 5.0 87 5.0 88 8.0 89 5.5 90 2.5 91 2.0 92 0.5 93 0.5 94 2.0 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26807022814132414 2 0.23761244346534488 3 0.22537369893801074 4 0.22975461317222695 5 0.23302291426759458 6 0.26208993252001317 7 0.2649410036883125 8 0.25214595259155415 9 0.24512258215257263 10-11 0.2553447153657437 12-13 0.2572570191981397 14-15 0.2408807445607125 16-17 0.25753517248285185 18-19 0.24477489054668247 20-21 0.2372995210200437 22-23 0.23653459948708533 24-25 0.22992845897517206 26-27 0.23900320988890555 28-29 0.25610963689870214 30-31 0.2582653248552212 32-33 0.25791763324933104 34-35 0.2642803896371212 36-37 0.23622167704178418 38-39 0.23496998726057955 40-41 0.23180599364697896 42-43 0.23688229109297554 44-45 0.2583696323369883 46-47 0.2634459297829848 48-49 0.2682440739442692 50-51 0.26987822449195303 52-53 0.29105264329066466 54-55 0.26786161317779 56-57 0.23430937320938822 58-59 0.21633371718486624 60-61 0.21271772448360843 62-63 0.2199844790467131 64-65 0.21257864784125238 66-67 0.21389987594363502 68-69 0.21782879109019399 70-71 0.21633371718486624 72-73 0.22109709218556162 74-75 0.22377431755091598 76-77 0.23907274821008362 78-79 0.23156260952285584 80-81 0.22554754474095584 82-83 0.22050601645554835 84-85 0.22158386043380787 86-87 0.2301023047781171 88-89 0.22666015787980437 90-91 0.23736905934122177 92-93 0.2240872399962171 94-95 0.22318324182090266 96-97 0.23382260496114196 98-99 0.24091551372130154 100-101 0.21310018525008764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1438056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.83174134538245 #Duplication Level Percentage of deduplicated Percentage of total 1 70.27214694325374 30.801505686014057 2 14.437939938413477 12.65680097881413 3 5.771370785966322 7.589076944963175 4 2.966223427293914 5.200589521510427 5 1.731363386024946 3.7944336055555494 6 1.089725156964405 2.8658730730572053 7 0.7126273187895303 2.1864987418985264 8 0.5112944785116891 1.7928741866757252 9 0.3759540452358809 1.483084842167641 >10 1.9127307386310473 15.140920628328642 >50 0.1387512212389006 4.18016134561849 >100 0.07147329478598548 5.714604494293897 >500 0.0053882076653275195 1.638842812144288 >1k 0.00237715044058567 1.9863666789334995 >5k 3.1695339207808937E-4 0.900033328400523 >10k+ 3.1695339207808937E-4 2.068333131624139 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17022 1.1836813030925082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12754 0.8868917483046558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7842 0.5453195146781488 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5115 0.35568851282564795 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3468 0.24115889784542466 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2981 0.2072937354317217 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2883 0.2004789799562743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2535 0.1762796441863182 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2182 0.15173261681047193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2107 0.1465172427221193 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1750 0.12169206206156089 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1605 0.11160900549074584 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1522 0.10583732483296895 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1509 0.10493332665765451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.95383211780348E-5 0.0 20-21 0.0 0.0 0.0 6.95383211780348E-5 0.0 22-23 0.0 0.0 0.0 1.73845802945087E-4 0.0 24-25 0.0 0.0 0.0 4.519990876572261E-4 0.0 26-27 0.0 0.0 0.0 0.0013212281023826612 0.0 28-29 0.0 0.0 0.0 0.002572917883587287 0.0 30-31 0.0 0.0 0.0 0.005841218978954923 0.0 32-33 0.0 0.0 0.0 0.010187364052582097 0.0 34-35 0.0 0.0 0.0 0.013872895075017941 0.0 36-37 0.0 0.0 0.0 0.016341505476838176 0.0 38-39 0.0 0.0 0.0 0.018914423360425463 0.0 40-41 0.0 0.0 0.0 0.02270426186462836 0.0 42-43 0.0 0.0 0.0 0.027537175186501776 0.0 44-45 0.0 0.0 0.0 0.032370088508375194 0.0 46-47 0.0 0.0 0.0 0.03946299726853474 0.0 48-49 0.0 0.0 0.0 0.04951128467876077 3.47691605890174E-5 50-51 0.0 0.0 0.0 0.06592232847677698 6.95383211780348E-5 52-53 0.0 0.0 0.0 0.10096964235050651 6.95383211780348E-5 54-55 0.0 0.0 0.0 0.14432678560501122 6.95383211780348E-5 56-57 0.0 0.0 0.0 0.193872839444361 6.95383211780348E-5 58-59 0.0 0.0 0.0 0.2664013084330513 6.95383211780348E-5 60-61 0.0 0.0 0.0 0.3687964863677075 6.95383211780348E-5 62-63 0.0 0.0 0.0 0.4480006341894891 6.95383211780348E-5 64-65 0.0 0.0 0.0 0.5287693942377765 6.95383211780348E-5 66-67 0.0 0.0 0.0 0.6633260457172738 6.95383211780348E-5 68-69 0.0 0.0 0.0 0.8377281552317852 6.95383211780348E-5 70-71 0.0 0.0 0.0 0.9909210767869958 6.95383211780348E-5 72-73 0.0 0.0 0.0 1.1428275394004128 1.0430748176705218E-4 74-75 0.0 0.0 0.0 1.3803356753839906 1.390766423560696E-4 76-77 0.0 0.0 0.0 1.576955278514884 1.390766423560696E-4 78-79 0.0 0.0 0.0 1.7579982977018975 1.390766423560696E-4 80-81 0.0 0.0 0.0 1.9734627858720382 1.390766423560696E-4 82-83 0.0 0.0 0.0 2.322267004901061 1.390766423560696E-4 84-85 0.0 0.0 0.0 2.673783218456027 1.390766423560696E-4 86-87 0.0 0.0 0.0 3.0550270643146025 1.390766423560696E-4 88-89 0.0 0.0 0.0 3.51936920398093 1.390766423560696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18880 0.0 31.184452 1 GGTATCA 12775 0.0 26.819035 1 GTACATG 13235 0.0 25.815096 1 TATCAAC 22805 0.0 25.79102 2 ATCAACG 22895 0.0 25.730238 3 TCAACGC 22965 0.0 25.672497 4 CAACGCA 23070 0.0 25.513578 5 TACATGG 13430 0.0 25.293444 2 AACGCAG 23515 0.0 25.009682 6 GAGTACT 11220 0.0 24.95748 12-13 AGTACTT 11340 0.0 24.06505 12-13 ACATGGG 13540 0.0 23.823957 3 GTACTTT 11825 0.0 23.799854 14-15 CGACCGT 70 1.7828159E-5 23.746332 18-19 ACGCAGA 25685 0.0 22.600819 7 AGAGTAC 18635 0.0 22.584688 10-11 CGCAGAG 25820 0.0 22.462685 8 CATGGGG 4100 0.0 21.89983 4 GCAGAGT 26110 0.0 21.849342 9 TACTTTT 12480 0.0 21.751474 14-15 >>END_MODULE