FastQCFastQC Report
Thu 26 May 2016
SRR937516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438056
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205601.4297078834203953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171891.1952942027292401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107990.7509443304015978No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58890.40951117341744686No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38500.26772253653543393No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30280.21056203652708932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.20284328287632747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28830.2004789799562743No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24680.17162057666738986No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16970.11800653103912503No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16700.1161289963673181No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16310.11341700184137474No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.10535055658472271No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15130.10521147994236664No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14510.10090010402932847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148250.029.8198071
GTATCAA210350.029.1064971
GAGTACT142650.025.07568212-13
GTACATG125600.024.2218271
GTACTTT147500.024.18338214-15
TATCAAC255550.023.9239122
ATCAACG258050.023.6921373
TACATGG127400.023.677392
TCAACGC259900.023.541774
CAACGCA262950.023.2687075
AGTACTT143950.023.03420812-13
AACGCAG267050.022.943856
ACATGGG126450.022.3150143
AGAGTAC216750.021.66442310-11
ACTTTTT165650.021.66305516-17
GTATAGA3101.0913936E-1121.4675711
GTATAGG4000.021.3909021
TACTTTT155650.021.0709314-15
CATGGGG41600.021.0113544
GGTAAGG5250.020.8112473
Produced by FastQC (version 0.11.5)