##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1438056 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07771324621572 31.0 31.0 34.0 30.0 34.0 2 32.26090430414393 33.0 31.0 34.0 30.0 34.0 3 32.270879576316915 34.0 31.0 34.0 30.0 34.0 4 35.982664096530314 37.0 35.0 37.0 35.0 37.0 5 35.90551758763219 37.0 35.0 37.0 35.0 37.0 6 35.96980785171092 37.0 35.0 37.0 35.0 37.0 7 35.903429351847215 37.0 35.0 37.0 35.0 37.0 8 35.88883743053122 37.0 35.0 37.0 35.0 37.0 9 37.54210058579082 39.0 37.0 39.0 35.0 39.0 10-11 37.46319927735777 39.0 37.0 39.0 34.5 39.0 12-13 37.351070820607816 39.0 37.0 39.0 34.0 39.0 14-15 38.394665784920754 40.0 38.0 41.0 33.5 41.0 16-17 38.398317937549024 40.0 38.0 41.0 33.5 41.0 18-19 38.509169670722144 40.0 38.0 41.0 34.0 41.0 20-21 38.46689558681999 40.0 38.0 41.0 34.0 41.0 22-23 38.3217851043353 40.0 38.0 41.0 34.0 41.0 24-25 38.229063054568115 40.0 38.0 41.0 33.5 41.0 26-27 38.122025150620004 40.0 37.0 41.0 33.5 41.0 28-29 37.97925498033456 40.0 37.0 41.0 33.0 41.0 30-31 37.887665709819366 40.0 37.0 41.0 33.0 41.0 32-33 37.675397550582176 40.0 36.0 41.0 33.0 41.0 34-35 37.46671652564295 40.0 35.5 41.0 33.0 41.0 36-37 37.22363419783374 40.0 35.0 41.0 33.0 41.0 38-39 37.0472293846693 39.0 35.0 41.0 32.0 41.0 40-41 36.8036693285936 39.0 35.0 41.0 31.0 41.0 42-43 36.470905166419115 39.0 35.0 41.0 31.0 41.0 44-45 35.986238366238865 39.0 35.0 41.0 30.0 41.0 46-47 35.3683173673348 39.0 35.0 41.0 27.0 41.0 48-49 34.814602143449214 39.0 35.0 40.0 24.0 41.0 50-51 34.474540977541906 38.5 34.0 40.0 23.0 41.0 52-53 34.20295558726503 38.0 34.0 40.0 21.5 41.0 54-55 33.948731481945075 38.0 34.0 40.0 20.0 41.0 56-57 33.65188560111706 38.0 33.5 40.0 18.5 41.0 58-59 33.3660573718965 37.5 33.0 40.0 17.5 41.0 60-61 33.01943178846999 37.0 33.0 40.0 13.5 41.0 62-63 32.6594023459448 36.0 32.5 39.5 9.5 41.0 64-65 32.257786205822306 36.0 32.0 39.0 7.5 41.0 66-67 31.826715023615215 35.0 31.5 39.0 4.5 40.0 68-69 31.387166076981703 35.0 31.0 38.5 2.0 40.0 70-71 30.9656272078417 35.0 31.0 37.5 2.0 40.0 72-73 30.503817306141066 35.0 30.0 37.0 2.0 39.0 74-75 30.04478580806311 34.5 30.0 36.5 2.0 39.0 76-77 28.811546281925043 33.5 28.5 35.0 2.0 37.5 78-79 29.39115270893484 34.0 29.0 35.5 2.0 37.5 80-81 29.192131599882064 34.0 29.0 35.0 2.0 37.0 82-83 28.84136813865385 34.0 29.0 35.0 2.0 37.0 84-85 28.48578984406727 34.0 29.0 35.0 2.0 36.0 86-87 28.09125131427427 34.0 28.5 35.0 2.0 36.0 88-89 27.613785207252015 33.5 27.0 35.0 2.0 35.5 90-91 27.09535859521465 33.0 26.0 35.0 2.0 35.0 92-93 26.767158233059074 33.0 25.5 35.0 2.0 35.0 94-95 26.335479285924887 33.0 25.0 35.0 2.0 35.0 96-97 25.855993438364017 33.0 23.5 35.0 2.0 35.0 98-99 25.144241253469964 32.5 19.0 34.5 2.0 35.0 100-101 22.85288646617378 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0343173217818773 1101 2 0.060680522453488095 1101 3 0.06816414951924088 1101 4 0.04112654724121967 1101 5 -0.027535373594155033 1101 6 -0.02429978492741469 1101 7 -0.008806676428399385 1101 8 -0.008690261882755124 1101 9 -0.04831183462189159 1101 10-11 0.0026389643628306203 1101 12-13 -0.05659412017729437 1101 14-15 -0.13947414035501282 1101 16-17 -0.1829864563633734 1101 18-19 -0.10410790975831219 1101 20-21 -0.006887849688240522 1101 22-23 0.012162734973642841 1101 24-25 -0.11765264803462117 1101 26-27 -0.0732307239302159 1101 28-29 -0.2931958508410233 1101 30-31 -0.17025303629866784 1101 32-33 -0.10532123600484766 1101 34-35 -0.019958374454056127 1101 36-37 -0.1104719162745127 1101 38-39 -0.1040223911118403 1101 40-41 0.022482146295992322 1101 42-43 -0.05227161206909159 1101 44-45 -0.11887115065010079 1101 46-47 -0.19007491982524272 1101 48-49 -0.19793141246042723 1101 50-51 -0.12644899903316542 1101 52-53 -0.3007162713929077 1101 54-55 -0.35754414850499927 1101 56-57 -0.4071025965728836 1101 58-59 -0.37263047737582156 1101 60-61 -0.3034222154458419 1101 62-63 -0.2972477983218269 1101 64-65 -0.28640098071962683 1101 66-67 -0.2117603973445945 1101 68-69 -0.18770059736001699 1101 70-71 -0.09790980908853086 1101 72-73 -0.19625908033995287 1101 74-75 -0.2537362319462986 1101 76-77 -0.009846296181773795 1101 78-79 -0.1559594298340663 1101 80-81 -0.2235457413872446 1101 82-83 -0.1474214739900006 1101 84-85 -0.050915827564796956 1101 86-87 -0.11571856741698028 1101 88-89 0.00924538698257038 1101 90-91 0.06032806392200385 1101 92-93 0.04035155276655544 1101 94-95 0.1184967636614438 1101 96-97 0.10843113473235277 1101 98-99 0.28983151864210654 1101 100-101 0.7668726878675329 1102 1 -0.0747471695812294 1102 2 0.042453538748389974 1102 3 0.013667936280612025 1102 4 0.006703966285137142 1102 5 0.02893600152741982 1102 6 0.013055235999807735 1102 7 -0.09633738088577104 1102 8 -0.043581624328247415 1102 9 0.0158506237208087 1102 10-11 -0.10852723546951637 1102 12-13 -0.12023376302713729 1102 14-15 -0.09947546929942064 1102 16-17 -0.08259857984129582 1102 18-19 -0.1824853270497826 1102 20-21 -0.11862213674607602 1102 22-23 0.028293345382635948 1102 24-25 -0.026348690701041733 1102 26-27 -0.09595713370657677 1102 28-29 -0.09870812240132665 1102 30-31 -0.15034953810807394 1102 32-33 -0.03300584974935106 1102 34-35 -0.11667120855214108 1102 36-37 -0.3376487564244073 1102 38-39 -0.07821348747637558 1102 40-41 0.02518056061704499 1102 42-43 0.13385690999869126 1102 44-45 0.10755797572229397 1102 46-47 0.0020104860285456994 1102 48-49 0.18969136885957738 1102 50-51 0.10704251762828676 1102 52-53 0.0519092700424153 1102 54-55 0.047224206261823554 1102 56-57 -0.002060785937239018 1102 58-59 0.22339853450289127 1102 60-61 0.14843713842430617 1102 62-63 0.22658010191679523 1102 64-65 0.1303592718674338 1102 66-67 0.24732819505500814 1102 68-69 0.03471905991836266 1102 70-71 0.1817081266957068 1102 72-73 0.23996874746389807 1102 74-75 0.208084363479216 1102 76-77 0.014339396782414582 1102 78-79 0.07595341769949826 1102 80-81 0.07557372341325319 1102 82-83 0.17072635720193574 1102 84-85 0.17665129873165242 1102 86-87 0.23457511469367276 1102 88-89 0.3001593502452913 1102 90-91 0.05658255823675162 1102 92-93 0.10170424292973834 1102 94-95 0.27268863402656507 1102 96-97 0.1955324879190421 1102 98-99 0.24501651938690117 1102 100-101 0.7728901773601109 1103 1 0.035742789114109996 1103 2 0.09998502591952985 1103 3 -0.029452701805126935 1103 4 -3.9717925782412067E-4 1103 5 -0.015563154067024243 1103 6 -0.0828916173177916 1103 7 -0.02093094712582655 1103 8 -0.05079467904165824 1103 9 -0.12453943060600636 1103 10-11 -0.09148143409812093 1103 12-13 -0.09200213139143187 1103 14-15 -0.06780771745291503 1103 16-17 -0.12793705421409385 1103 18-19 -0.16915033203482466 1103 20-21 -0.30123755579504774 1103 22-23 -0.09639890439862597 1103 24-25 -0.07900449878698623 1103 26-27 -0.21195098887575625 1103 28-29 -0.24201035855001862 1103 30-31 -0.21907702910447568 1103 32-33 -0.16361656861315765 1103 34-35 -0.06926241452605097 1103 36-37 -0.12273991153052322 1103 38-39 -0.054211513805547895 1103 40-41 -0.005195148988832443 1103 42-43 -0.07736910583136591 1103 44-45 -0.07490229652219682 1103 46-47 0.11448683249933111 1103 48-49 -0.07006253270104423 1103 50-51 0.10896277725756676 1103 52-53 0.13072639011078024 1103 54-55 -0.08157561875292885 1103 56-57 -0.1928201405265355 1103 58-59 -0.08212621777089879 1103 60-61 -0.14153478254747398 1103 62-63 0.10522223673682873 1103 64-65 -0.07200557237535321 1103 66-67 0.17926452891058986 1103 68-69 0.09816392939157126 1103 70-71 0.13103870638520831 1103 72-73 0.30212157469648915 1103 74-75 0.3144489432094133 1103 76-77 0.3944516111596883 1103 78-79 0.23600133517157218 1103 80-81 0.08105981615543456 1103 82-83 0.1269240905325546 1103 84-85 0.13560606707966727 1103 86-87 0.2704877848464804 1103 88-89 0.4637552970986043 1103 90-91 0.516460577240963 1103 92-93 0.49805091719266414 1103 94-95 0.4119341627465296 1103 96-97 0.5037761243566727 1103 98-99 0.7139286605145401 1103 100-101 1.2415098286104396 1104 1 -0.01586411318879044 1104 2 0.01889093238076356 1104 3 0.026595201795537093 1104 4 -0.03286301350696874 1104 5 0.007297076343405706 1104 6 -0.05766954962988535 1104 7 -0.051962619924125875 1104 8 -0.11626279229987802 1104 9 -0.09967581516570334 1104 10-11 -0.11767858353922378 1104 12-13 -0.05865215017757208 1104 14-15 -0.12440281585634239 1104 16-17 -0.18301921626270712 1104 18-19 -0.1146210041556941 1104 20-21 -0.12894432846301385 1104 22-23 -0.20134769542414688 1104 24-25 -0.04733910215270498 1104 26-27 -0.19532102508754434 1104 28-29 -0.1655992303876559 1104 30-31 -0.09618042477865174 1104 32-33 -0.13614192637810874 1104 34-35 -0.07631655738108378 1104 36-37 -0.18198040161968976 1104 38-39 -0.02902081509505905 1104 40-41 -0.019610251494896147 1104 42-43 -0.042954165457103954 1104 44-45 -0.10445945115152 1104 46-47 0.11750688961202371 1104 48-49 0.13492457077217068 1104 50-51 0.024778310752232358 1104 52-53 0.19677024746582106 1104 54-55 0.26909712245159767 1104 56-57 0.15721447108676045 1104 58-59 -0.0019901116549334574 1104 60-61 0.14502910901033061 1104 62-63 0.14287100146879084 1104 64-65 0.15038144467457215 1104 66-67 0.3101963496717737 1104 68-69 0.5007729471619236 1104 70-71 0.3821452558752334 1104 72-73 0.28679271855038735 1104 74-75 0.3260011859503109 1104 76-77 0.3754087033330258 1104 78-79 0.1999418144754479 1104 80-81 0.14237312597730778 1104 82-83 0.14876077588197134 1104 84-85 0.25043079908639143 1104 86-87 0.0788564640856464 1104 88-89 0.30670633541176073 1104 90-91 0.3450648903696205 1104 92-93 0.3381144235112217 1104 94-95 0.552180102578717 1104 96-97 0.5402069621057457 1104 98-99 0.6857550892657223 1104 100-101 0.8960231900325191 1105 1 -0.03534138225935379 1105 2 -0.11012852769863457 1105 3 -0.07963178983378327 1105 4 -0.1111660769808509 1105 5 -0.1306321324780555 1105 6 -0.12007698326745242 1105 7 -0.11711696251558834 1105 8 -0.13671265278853184 1105 9 -0.04126841728264452 1105 10-11 -0.060756029182059024 1105 12-13 1.6178335479821726E-4 1105 14-15 -0.09155164125194659 1105 16-17 -0.10287703020448902 1105 18-19 -0.05551553385325292 1105 20-21 -0.0070584087263867445 1105 22-23 -0.13004924978268662 1105 24-25 -0.15538751100827142 1105 26-27 -0.08218842811375282 1105 28-29 -0.04570232714073086 1105 30-31 -0.035302699700579865 1105 32-33 -0.09763689805641462 1105 34-35 -0.09739005902647335 1105 36-37 -0.10044000796253982 1105 38-39 -0.3384891592899706 1105 40-41 -0.391051130725792 1105 42-43 -0.31263616347409595 1105 44-45 -0.23636196235574403 1105 46-47 -0.3993113097083736 1105 48-49 -0.21019153512168742 1105 50-51 -0.12258475979004402 1105 52-53 -0.20409934346133696 1105 54-55 0.05581786323003257 1105 56-57 0.015960092878302135 1105 58-59 0.013493997528343016 1105 60-61 -0.08363129328291308 1105 62-63 0.04933840779541043 1105 64-65 0.016448661777324958 1105 66-67 0.09300953510978971 1105 68-69 0.16018545931502004 1105 70-71 0.04812439311891836 1105 72-73 0.24798188603413962 1105 74-75 0.21500096729773333 1105 76-77 0.3955266313316912 1105 78-79 0.16176098329311728 1105 80-81 0.13796208525979736 1105 82-83 -0.07540848184065752 1105 84-85 -0.047602462233694354 1105 86-87 0.14313602447019846 1105 88-89 0.26134400471280017 1105 90-91 0.3634485527590421 1105 92-93 0.3471692530515966 1105 94-95 0.45678242992225293 1105 96-97 0.4049210085398549 1105 98-99 0.4873060442549182 1105 100-101 0.7830666152595249 1106 1 -0.2386146311155919 1106 2 -0.15880210751574708 1106 3 -0.063723622887494 1106 4 -0.025810523079464076 1106 5 -0.17582432454988606 1106 6 -0.10930071396378338 1106 7 -0.0578303034453711 1106 8 -0.10731410832926258 1106 9 -0.11983583767739248 1106 10-11 -0.24830076329679684 1106 12-13 -0.2296706462301259 1106 14-15 -0.2502410819967764 1106 16-17 -0.11182743188105349 1106 18-19 0.04762916705087861 1106 20-21 -0.030611456944747317 1106 22-23 -0.10160551026835662 1106 24-25 -0.05343595841240756 1106 26-27 0.03262006693042707 1106 28-29 -0.046714391752537665 1106 30-31 -0.05652313224010186 1106 32-33 -0.09266532748100786 1106 34-35 -0.05570931602503748 1106 36-37 -0.15565478897293872 1106 38-39 -0.20569325564653695 1106 40-41 -0.017445403613912447 1106 42-43 -0.21999216972685076 1106 44-45 -0.018897620813284277 1106 46-47 -0.3771010006779463 1106 48-49 -0.5262971047694762 1106 50-51 -0.39947103636568926 1106 52-53 -0.4003675878226929 1106 54-55 -0.2954107860716846 1106 56-57 -0.10231046477359484 1106 58-59 -0.2845010552364471 1106 60-61 -0.25417849528928826 1106 62-63 -0.19924441544971927 1106 64-65 -0.24170511478399703 1106 66-67 -0.17996375898158945 1106 68-69 -0.2176904074531869 1106 70-71 -0.25220542256221634 1106 72-73 -0.3261221433286181 1106 74-75 -0.22792044953546053 1106 76-77 -0.1682606375539777 1106 78-79 -0.08835138880310112 1106 80-81 -0.0978124662538633 1106 82-83 -0.17220093845214635 1106 84-85 -0.2502129304459899 1106 86-87 0.0030745848476563253 1106 88-89 0.265457396031092 1106 90-91 0.10218557628703806 1106 92-93 0.28258893777987026 1106 94-95 0.36971409623581764 1106 96-97 0.4993858596933478 1106 98-99 0.3936664480500909 1106 100-101 1.0770499126147008 1107 1 -0.009916804889762432 1107 2 -0.05106874475738721 1107 3 -0.03726722904119839 1107 4 0.008121667710888403 1107 5 -0.12725414875468033 1107 6 -0.07581135053174393 1107 7 -0.07317536033053784 1107 8 -0.02782587156617211 1107 9 -0.09105786695916862 1107 10-11 -0.0029221386459710175 1107 12-13 -0.05664856003414798 1107 14-15 -0.0405057040109611 1107 16-17 -0.0765791001060876 1107 18-19 -0.06783842960164321 1107 20-21 0.06904357328365762 1107 22-23 0.09274052809645639 1107 24-25 0.028360062239322303 1107 26-27 -0.22226627888725403 1107 28-29 -0.08898335202294305 1107 30-31 -0.04821177088717121 1107 32-33 -0.06613779087879124 1107 34-35 -0.0964617740798488 1107 36-37 -0.08882705897857335 1107 38-39 0.014787582269939037 1107 40-41 0.10533029634369484 1107 42-43 0.06871094138942624 1107 44-45 0.1801605362118508 1107 46-47 0.1287639210069429 1107 48-49 0.11202764303671131 1107 50-51 0.059425147478812335 1107 52-53 0.03727143092995533 1107 54-55 0.08825846690033501 1107 56-57 0.2642794451509687 1107 58-59 0.09979251171614578 1107 60-61 0.17952879727876336 1107 62-63 0.25436978174057145 1107 64-65 0.13326584735643365 1107 66-67 0.1749405903090171 1107 68-69 0.4666571699181894 1107 70-71 0.6138726950875757 1107 72-73 0.6246180317115986 1107 74-75 0.7033977531371818 1107 76-77 0.6060658292934953 1107 78-79 0.5187119253458832 1107 80-81 0.5876400016738224 1107 82-83 0.4088843805868194 1107 84-85 0.3854991359461728 1107 86-87 0.3865761441807116 1107 88-89 0.41716932304152365 1107 90-91 0.4771804946616136 1107 92-93 0.4812441945035886 1107 94-95 0.321698543558238 1107 96-97 0.5760501646141059 1107 98-99 0.6916420736707742 1107 100-101 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0.2878120053080693 2316 8 0.17588587644902276 2316 9 0.30443137950792476 2316 10-11 0.2722021716398899 2316 12-13 0.214872369381645 2316 14-15 0.22888138620411524 2316 16-17 0.24238422604898346 2316 18-19 0.19954305971877062 2316 20-21 0.23880336329632001 2316 22-23 0.4470028921176379 2316 24-25 0.44468630048992 2316 26-27 0.6348207937672612 2316 28-29 0.6886537358815303 2316 30-31 0.41093450858453195 2316 32-33 0.6002496782747002 2316 34-35 0.4150531015636503 2316 36-37 0.4677433634915502 2316 38-39 0.43616479235894445 2316 40-41 0.5201153643215477 2316 42-43 0.49281928080733906 2316 44-45 0.02305128179629179 2316 46-47 -0.23323202157926204 2316 48-49 -0.785920081946422 2316 50-51 -0.560360983375098 2316 52-53 -0.1529279276474469 2316 54-55 -0.2363196175197544 2316 56-57 -0.3267986346479148 2316 58-59 0.00742049585618787 2316 60-61 -0.10057832214229023 2316 62-63 -0.17401085727899357 2316 64-65 -0.13880038368508707 2316 66-67 -0.22245374594799472 2316 68-69 0.031573479982238695 2316 70-71 0.012525153103975839 2316 72-73 -0.09063747444846015 2316 74-75 -0.23389140460286484 2316 76-77 -0.3768207109987465 2316 78-79 -0.6062487944522594 2316 80-81 -0.4776679551087142 2316 82-83 -0.5142280901840905 2316 84-85 -0.6561748476208393 2316 86-87 -1.11285660349251 2316 88-89 -0.7252526017807917 2316 90-91 -0.7180721463543129 2316 92-93 -0.5887947160022478 2316 94-95 -0.6771045655852674 2316 96-97 -0.48339695499180024 2316 98-99 -0.17136475646314508 2316 100-101 -0.533911624017307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 15.0 5 60.0 6 120.0 7 267.0 8 580.0 9 1210.0 10 2000.0 11 3374.0 12 5219.0 13 7310.0 14 9411.0 15 13386.0 16 19438.0 17 27805.0 18 23654.0 19 12706.0 20 9819.0 21 9832.0 22 10101.0 23 10637.0 24 12075.0 25 13738.0 26 16492.0 27 19870.0 28 24129.0 29 29320.0 30 35882.0 31 44697.0 32 55404.0 33 70957.0 34 96753.0 35 138088.0 36 196336.0 37 280436.0 38 218417.0 39 18510.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33988128788037 20.021988880306733 16.43035578843357 19.20777404337933 2 15.816073922016946 22.372007766039708 38.97010964802483 22.84180866391851 3 19.108296199869823 27.66644692557174 31.409347062979464 21.815909811578965 4 12.613208386877842 18.472159637733164 37.44979333210946 31.464838643279535 5 12.372558523462583 39.298392990883954 32.77592066274783 15.553127822905635 6 28.679550726814533 38.08759881395439 16.991827856495156 16.241022602735917 7 26.01630256401698 36.582859081982896 20.159785154402886 17.241053199597232 8 27.473617160945054 32.782589829603296 19.31253024916971 20.43126276028194 9 27.961428484008966 16.311673537052798 20.405116351518995 35.32178162741924 10-11 25.001321229939904 27.956877836298222 25.41573543135376 21.626065502408114 12-13 27.44082655065175 26.04007299148247 26.834662052774178 19.6844384050916 14-15 23.706900148533855 25.996901372408303 28.056730753183462 22.239467725874377 16-17 20.30060025478841 29.720678471492068 28.12866814644214 21.85005312727738 18-19 20.329562965559063 26.39692056498495 33.369423722024735 19.904092747431253 20-21 21.975952257770214 24.61225432111128 33.7760490551133 19.635744366005216 22-23 21.433838983872935 24.999956538322596 33.981086173380106 19.585118304424366 24-25 21.118082393253328 25.206556829857252 34.0407533157052 19.634607461184213 26-27 20.401481172420986 25.463196689962103 34.2525642362922 19.88275790132471 28-29 20.213988827685863 26.115023514988327 33.975559432803045 19.69542822452276 30-31 20.34011334793644 25.51124787038003 33.90163763429644 20.247001147387085 32-33 20.158985463709342 25.728692067624625 33.72778946021573 20.384533008450298 34-35 20.946402643568817 25.627027042062338 33.522408028616404 19.904162285752435 36-37 20.94481054451654 25.3329235206847 33.59261864371716 20.129647291081604 38-39 20.52849436475499 25.86764345405837 33.3871794916371 20.216682689549543 40-41 20.536120340404462 25.952293863790928 33.192425319216106 20.3191604765885 42-43 20.55271630709228 26.14452313665844 32.4515841198208 20.851176436428478 44-45 20.824406332729044 26.04538914768788 32.26070249149371 20.869502028089368 46-47 21.24353988996256 26.75090538894174 31.424158725390388 20.58139599570531 48-49 21.263462618980068 26.967899720177797 30.663444260863276 21.105193399978862 50-51 21.106479858920654 27.48641221204181 30.390923580166557 21.016184348870976 52-53 21.582643513187246 27.805801721212525 29.41384062929399 21.197714136306235 54-55 20.99055947751687 27.658241403672733 29.65308722330702 21.698111895503374 56-57 21.48129141007026 27.638735904582294 29.40257542126315 21.477397264084292 58-59 21.901441946627948 27.117546187352925 29.52138164299582 21.459630223023304 60-61 21.4137349310458 27.445732294152663 30.31728249803902 20.82325027676252 62-63 21.429415822471448 27.565651129024182 30.18950583287438 20.815427215629988 64-65 21.168681887214404 27.791998364458685 29.919766685024783 21.119553063302124 66-67 21.446487480320656 27.570762195630767 29.454868238789032 21.527882085259545 68-69 21.402295877212012 27.56120067646879 29.715915096491376 21.320588349827823 70-71 21.788963712122477 27.769954716645252 29.27775413474858 21.163327436483698 72-73 21.510879965731515 27.805175876321925 29.364329344615232 21.31961481333133 74-75 20.933746669114417 28.65823723137347 29.035899853691372 21.372116245820745 76-77 21.075882997602317 27.897627074328117 29.491375857407498 21.535114070662058 78-79 21.073727309645797 28.097793131839094 29.282726124712806 21.5457534338023 80-81 21.29257180964156 28.245884807644767 29.004548155478005 21.456995227235666 82-83 21.14943112220329 28.02846631602993 29.233535261800615 21.58856729996617 84-85 20.898667710672292 28.245451656443336 28.963854550360175 21.892026082524193 86-87 20.894492286809417 28.16660130064476 28.66557352425775 22.273332888288078 88-89 20.88659968735571 27.96629616649143 28.54829714559099 22.59880700056187 90-91 20.85503091905966 27.88268421389369 28.753682330654517 22.508602536392136 92-93 21.138453642945958 27.81600570788005 28.954695788352254 22.090844860821747 94-95 20.69995883331386 27.65997986170219 28.95593078433663 22.68413052064732 96-97 20.489640180910897 27.77861223763192 29.205190896599298 22.52655668485789 98-99 20.46094219655792 27.990350852488554 28.582053044582107 22.96665390637142 100-101 20.120612262142334 27.673187573522327 29.20290406898607 23.003296095349267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2272.0 1 1563.5 2 1114.5 3 1423.5 4 1158.5 5 1031.5 6 1963.5 7 4255.5 8 7110.5 9 8547.0 10 7126.0 11 5169.5 12 4790.5 13 4936.0 14 5142.5 15 5215.0 16 5292.5 17 5412.5 18 5431.0 19 5516.0 20 5816.0 21 6196.5 22 6812.5 23 7593.5 24 8403.0 25 9440.5 26 10944.5 27 12702.5 28 14243.5 29 16439.0 30 18882.0 31 20994.0 32 23924.0 33 25878.0 34 27358.5 35 29097.5 36 31611.5 37 34946.0 38 37217.0 39 38164.0 40 39301.0 41 42297.0 42 44608.5 43 46427.0 44 49282.0 45 51294.5 46 50632.5 47 54393.5 48 62760.0 49 60434.5 50 51074.5 51 44733.5 52 40368.5 53 39206.0 54 38918.0 55 36162.5 56 32623.5 57 29595.0 58 27859.5 59 25549.5 60 21587.5 61 18484.5 62 16001.0 63 11894.5 64 7990.5 65 6030.0 66 4436.5 67 3493.5 68 2735.5 69 1905.0 70 1339.5 71 1027.5 72 891.0 73 789.0 74 595.0 75 450.0 76 330.0 77 195.5 78 117.0 79 78.0 80 56.0 81 39.5 82 28.0 83 18.0 84 10.0 85 6.5 86 3.0 87 0.5 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06717401825798161 2 0.0 3 0.0 4 0.0 5 0.002990147810655496 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3907664235606956E-4 12-13 0.005563065694242783 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.21537408835261E-4 24-25 9.387673359034697E-4 26-27 4.1722992706820874E-4 28-29 0.002572917883587287 30-31 4.1722992706820874E-4 32-33 0.0 34-35 0.0 36-37 3.476916058901739E-5 38-39 5.910757300132957E-4 40-41 1.3907664235606956E-4 42-43 0.012203975366745105 44-45 2.0861496353410437E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.476916058901739E-5 82-83 3.8246076647919136E-4 84-85 3.4769160589017393E-4 86-87 0.0 88-89 0.0 90-91 0.007510138687227758 92-93 0.003059686131833531 94-95 0.0 96-97 0.0 98-99 1.0430748176705218E-4 100-101 0.009909210767869958 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1438056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.11191419756715 #Duplication Level Percentage of deduplicated Percentage of total 1 68.13908238047206 28.013281083269266 2 15.21525983961075 12.510569140395331 3 6.182744075345758 7.625533317933943 4 3.21629294866062 5.289118389583026 5 1.9114580427845567 3.929184952360417 6 1.1714262713426487 2.8895745813728992 7 0.8179925669700829 2.3540468159265227 8 0.5561733125730145 1.8292279604382775 9 0.42761445216523325 1.5822043800351075 >10 2.1183357276738004 15.775406489460122 >50 0.15515607168936757 4.392087624683352 >100 0.07780802587245453 5.780708606935687 >500 0.006427600379535032 1.6916928919935954 >1k 0.0035520949465851492 2.5518297514941004 >5k 1.6914737840881665E-4 0.4095194409894473 >10k+ 5.074421352264499E-4 3.376014573128831 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20560 1.4297078834203953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17189 1.1952942027292401 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10799 0.7509443304015978 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5889 0.40951117341744686 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3850 0.26772253653543393 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3028 0.21056203652708932 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2917 0.20284328287632747 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2883 0.2004789799562743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2468 0.17162057666738986 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1697 0.11800653103912503 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1670 0.1161289963673181 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1631 0.11341700184137474 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1515 0.10535055658472271 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1513 0.10521147994236664 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1451 0.10090010402932847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.95383211780348E-5 2 0.0 0.0 0.0 0.0 6.95383211780348E-5 3 0.0 0.0 0.0 0.0 6.95383211780348E-5 4 0.0 0.0 0.0 0.0 6.95383211780348E-5 5 0.0 0.0 0.0 0.0 6.95383211780348E-5 6 0.0 0.0 0.0 0.0 6.95383211780348E-5 7 0.0 0.0 0.0 0.0 6.95383211780348E-5 8 0.0 0.0 0.0 0.0 6.95383211780348E-5 9 0.0 0.0 0.0 0.0 6.95383211780348E-5 10-11 0.0 0.0 0.0 0.0 6.95383211780348E-5 12-13 0.0 0.0 0.0 0.0 6.95383211780348E-5 14-15 0.0 0.0 0.0 0.0 6.95383211780348E-5 16-17 0.0 0.0 0.0 0.0 6.95383211780348E-5 18-19 0.0 0.0 0.0 6.95383211780348E-5 6.95383211780348E-5 20-21 0.0 0.0 0.0 6.95383211780348E-5 6.95383211780348E-5 22-23 0.0 0.0 0.0 1.73845802945087E-4 6.95383211780348E-5 24-25 0.0 0.0 0.0 4.519990876572261E-4 6.95383211780348E-5 26-27 0.0 0.0 0.0 0.0013212281023826612 6.95383211780348E-5 28-29 0.0 0.0 0.0 0.002572917883587287 6.95383211780348E-5 30-31 0.0 0.0 0.0 0.0058064498183659045 6.95383211780348E-5 32-33 0.0 0.0 0.0 0.010152594891993081 6.95383211780348E-5 34-35 0.0 0.0 0.0 0.013838125914428925 6.95383211780348E-5 36-37 0.0 0.0 0.0 0.01641104379801621 6.95383211780348E-5 38-39 0.0 0.0 0.0 0.019018730842192515 6.95383211780348E-5 40-41 0.0 0.0 0.0 0.022912876828162464 6.95383211780348E-5 42-43 0.0 0.0 0.0 0.027884866792391952 6.95383211780348E-5 44-45 0.0 0.0 0.0 0.03292639507779947 1.390766423560696E-4 46-47 0.0 0.0 0.0 0.04026268796208214 1.390766423560696E-4 48-49 0.0 0.0 0.0 0.05031097537230817 1.390766423560696E-4 50-51 0.0 0.0 0.0 0.0669306341338585 1.390766423560696E-4 52-53 0.0 0.0 0.0 0.10197794800758803 1.390766423560696E-4 54-55 0.0 0.0 0.0 0.14557847538621582 1.390766423560696E-4 56-57 0.0 0.0 0.0 0.1951245292255656 1.390766423560696E-4 58-59 0.0 0.0 0.0 0.2682440739442692 1.390766423560696E-4 60-61 0.0 0.0 0.0 0.37067402103951447 1.390766423560696E-4 62-63 0.0 0.0 0.0 0.449669553897762 1.390766423560696E-4 64-65 0.0 0.0 0.0 0.5307164672307616 1.390766423560696E-4 66-67 0.0 0.0 0.0 0.6651688112284918 1.390766423560696E-4 68-69 0.0 0.0 0.0 0.8408921488453858 1.390766423560696E-4 70-71 0.0 0.0 0.0 0.995406298502979 1.390766423560696E-4 72-73 0.0 0.0 0.0 1.1487035275399566 1.390766423560696E-4 74-75 0.0 0.0 0.0 1.3884716589618207 1.390766423560696E-4 76-77 0.0 0.0 0.0 1.587038335085699 1.390766423560696E-4 78-79 0.0 0.0 0.0 1.7718016544557376 1.390766423560696E-4 80-81 6.95383211780348E-5 0.0 0.0 1.9903953670788899 1.390766423560696E-4 82-83 6.95383211780348E-5 0.0 0.0 2.345110343408045 1.390766423560696E-4 84-85 6.95383211780348E-5 0.0 0.0 2.702606852584322 1.390766423560696E-4 86-87 6.95383211780348E-5 0.0 0.0 3.0894137641371406 1.390766423560696E-4 88-89 6.95383211780348E-5 0.0 0.0 3.5630740388413247 1.390766423560696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14825 0.0 29.819807 1 GTATCAA 21035 0.0 29.106497 1 GAGTACT 14265 0.0 25.075682 12-13 GTACATG 12560 0.0 24.221827 1 GTACTTT 14750 0.0 24.183382 14-15 TATCAAC 25555 0.0 23.923912 2 ATCAACG 25805 0.0 23.692137 3 TACATGG 12740 0.0 23.67739 2 TCAACGC 25990 0.0 23.54177 4 CAACGCA 26295 0.0 23.268707 5 AGTACTT 14395 0.0 23.034208 12-13 AACGCAG 26705 0.0 22.94385 6 ACATGGG 12645 0.0 22.315014 3 AGAGTAC 21675 0.0 21.664423 10-11 ACTTTTT 16565 0.0 21.663055 16-17 GTATAGA 310 1.0913936E-11 21.467571 1 GTATAGG 400 0.0 21.390902 1 TACTTTT 15565 0.0 21.07093 14-15 CATGGGG 4160 0.0 21.011354 4 GGTAAGG 525 0.0 20.811247 3 >>END_MODULE