FastQCFastQC Report
Thu 26 May 2016
SRR937515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458759
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.8357329229508303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.6079444762936531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.44380600707561046No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8710.18986003544344635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8370.1824487367005334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8240.17961500482824314No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7000.15258556235409004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.14539224298596867No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5160.11247735739244352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.10332222365119813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA756.8982554E-638.0119442
GGTATCA25050.035.86921
GTATCAA38950.033.321371
CATATAG1102.7220267E-630.2367733
GTACATG28900.028.7877581
GTTCTAG1703.596142E-927.9652521
GGGAACG700.008373531527.1513887
TATCAAC47700.027.0944672
TACATGG30300.026.658542
ATCAACG48750.026.5108933
TCAACGC49000.026.4726054
ACATGGG30300.026.1880973
CAACGCA49500.026.0160685
AACGCAG50350.025.668446
GTAAGCG651.0031981E-525.57237694-95
GAGTACT27000.024.45336512-13
CATGGGG10450.023.1891064
ACGCAGA55150.023.1759137
AGTACTT28550.023.12577412-13
CGCAGAG55450.022.9648348
Produced by FastQC (version 0.11.5)