FastQCFastQC Report
Thu 26 May 2016
SRR937515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458759
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.9968196809217912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.8006382436093896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.5813509925690831No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12110.2639730228725758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9780.21318382854614298No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.20359273605531444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.14691809860950955No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6240.1360191298699317No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG4920.10724585239744616No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTA405.3858705E-447.498042
GGACCAT1106.999471E-834.544036
GGTATCA32900.032.7864951
ACGCGAA300.00395133931.6653678-79
GTATCAA47150.027.9166371
GTACAAC700.00838809727.1417356
AGTGTCG700.00838809727.1417355
AGCGCTC1109.467124E-525.908028
AGTATAC1000.001652533723.749025
GTATATA3150.022.6279771
ATCAACG58500.022.5717163
TATCAAC58150.022.544212
TCAACGC58900.022.418434
GAGTACT35300.022.33618712-13
CAACGCA59400.022.2297235
CCATATA1503.0290556E-522.165752
GTACATG25900.022.016411
AACGCAG60300.021.8979366
GTGTAGG1754.026666E-621.722861
AGGTAAG1754.0419236E-621.7133882
Produced by FastQC (version 0.11.5)