##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 458759 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09364829899795 31.0 31.0 34.0 30.0 34.0 2 32.29534461449258 33.0 31.0 34.0 31.0 34.0 3 32.33547243759796 34.0 31.0 34.0 30.0 34.0 4 36.01183845984493 37.0 35.0 37.0 35.0 37.0 5 35.93264655298316 37.0 35.0 37.0 35.0 37.0 6 35.98139981994904 37.0 35.0 37.0 35.0 37.0 7 35.91509703351869 37.0 35.0 37.0 35.0 37.0 8 35.894707242800685 37.0 35.0 37.0 35.0 37.0 9 37.55478802595698 39.0 37.0 39.0 35.0 39.0 10-11 37.46611510618865 39.0 37.0 39.0 35.0 39.0 12-13 37.34471258329537 39.0 37.0 39.0 34.0 39.0 14-15 38.47528440858926 40.0 38.0 41.0 34.0 41.0 16-17 38.44636072534817 40.0 38.0 41.0 34.0 41.0 18-19 38.4855196301326 40.0 38.0 41.0 34.0 41.0 20-21 38.41198864763416 40.0 38.0 41.0 34.0 41.0 22-23 38.345906020372354 40.0 38.0 41.0 34.0 41.0 24-25 38.33732744207743 40.0 38.0 41.0 34.0 41.0 26-27 38.144755743211576 40.0 37.5 41.0 33.5 41.0 28-29 37.971956953433065 40.0 37.0 41.0 33.0 41.0 30-31 37.926027609267614 40.0 37.0 41.0 33.0 41.0 32-33 37.734385592435245 40.0 36.5 41.0 33.0 41.0 34-35 37.5502257176426 40.0 36.0 41.0 33.0 41.0 36-37 37.328533064201466 40.0 36.0 41.0 33.0 41.0 38-39 37.15901050442607 40.0 35.5 41.0 32.0 41.0 40-41 36.898394363925284 39.0 35.0 41.0 31.0 41.0 42-43 36.60048413219141 39.0 35.0 41.0 31.0 41.0 44-45 36.15202753515462 39.0 35.0 41.0 30.0 41.0 46-47 35.59744114011932 39.0 35.0 40.5 28.0 41.0 48-49 35.10351731519164 39.0 35.0 40.0 26.0 41.0 50-51 34.78520530387415 38.5 34.5 40.0 24.0 41.0 52-53 34.48287336052263 38.0 34.0 40.0 23.5 41.0 54-55 34.22659827927082 38.0 34.0 40.0 23.0 41.0 56-57 33.87527547143489 38.0 33.5 40.0 22.0 41.0 58-59 33.58427845557253 37.5 33.0 40.0 20.5 41.0 60-61 33.19902061866906 37.0 33.0 40.0 18.5 41.0 62-63 32.79896743170161 36.0 32.5 39.5 17.0 41.0 64-65 32.354975052260556 36.0 32.0 39.0 12.5 40.5 66-67 31.9131210504862 35.0 31.0 39.0 8.0 40.0 68-69 31.442565704433044 35.0 31.0 38.0 7.0 40.0 70-71 30.974901854786502 35.0 30.5 37.5 3.5 40.0 72-73 30.49045577307475 35.0 30.0 37.0 2.0 39.0 74-75 30.000794534821114 34.0 29.5 36.5 2.0 39.0 76-77 28.7225351437247 32.5 28.0 35.0 2.0 37.5 78-79 29.36098583352043 34.0 29.0 35.0 2.0 37.5 80-81 29.18198771032285 34.0 29.0 35.0 2.0 37.0 82-83 28.77698857134138 34.0 29.0 35.0 2.0 37.0 84-85 28.36781185764203 34.0 29.0 35.0 2.0 36.0 86-87 28.00871481540417 34.0 27.5 35.0 2.0 36.0 88-89 27.552791334883892 33.0 26.5 35.0 2.0 35.0 90-91 26.982496256204236 33.0 25.5 35.0 2.0 35.0 92-93 26.450087082760227 33.0 25.0 35.0 2.0 35.0 94-95 25.94357494893833 32.0 24.0 34.5 2.0 35.0 96-97 25.234028106260585 32.0 20.0 34.0 2.0 35.0 98-99 24.254041882557072 31.5 9.0 34.0 2.0 35.0 100-101 21.510557831018026 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18987511270556467 1101 2 0.25615162730949237 1101 3 0.2182516783504198 1101 4 0.07197483398164906 1101 5 0.13592885271175703 1101 6 0.182046412295918 1101 7 0.13702260907289343 1101 8 0.12344764655327367 1101 9 0.2011853782153139 1101 10-11 0.26551947404259835 1101 12-13 0.284583430108853 1101 14-15 0.31322049662249185 1101 16-17 0.22342514200099117 1101 18-19 0.34650079512812226 1101 20-21 0.2040858592353345 1101 22-23 0.23671330786706335 1101 24-25 0.3503292047219304 1101 26-27 0.3329010581013918 1101 28-29 0.31178280569431394 1101 30-31 0.535222589897991 1101 32-33 0.4630302247103444 1101 34-35 0.4357355213308267 1101 36-37 0.5394801210243614 1101 38-39 0.3053270157924004 1101 40-41 0.5154681537797146 1101 42-43 0.4193609363564761 1101 44-45 0.4023776578554319 1101 46-47 0.5269652396030367 1101 48-49 0.5801676015827724 1101 50-51 0.6450720198418267 1101 52-53 0.6207020466805133 1101 54-55 0.5434673771348884 1101 56-57 0.627490446433292 1101 58-59 0.5971680206456682 1101 60-61 0.6820278540850566 1101 62-63 0.8742620697514738 1101 64-65 0.8395027671730837 1101 66-67 0.7273734480715994 1101 68-69 0.8666452528285333 1101 70-71 0.9330586529547169 1101 72-73 0.7820676640577311 1101 74-75 0.806971738643675 1101 76-77 0.6716968345019758 1101 78-79 0.9563033829311927 1101 80-81 0.8818988360450213 1101 82-83 0.7100800374088792 1101 84-85 0.9964169403277268 1101 86-87 0.9747888912457938 1101 88-89 1.1488587024352768 1101 90-91 1.1376053787065565 1101 92-93 1.2125649108620031 1101 94-95 1.3046853540367067 1101 96-97 1.7324370286722193 1101 98-99 1.6564508332503287 1101 100-101 2.12140106367994 1102 1 0.13816217377854656 1102 2 0.1907079602319044 1102 3 0.2690760709483868 1102 4 0.07568225637393056 1102 5 0.05880257392548316 1102 6 -0.028455587790993775 1102 7 0.13425519322177593 1102 8 0.056437301907081405 1102 9 0.1754861482888046 1102 10-11 0.03420353349859084 1102 12-13 0.031193345691228558 1102 14-15 0.06251320459607967 1102 16-17 0.1813804333687159 1102 18-19 0.14588258644667462 1102 20-21 0.1191141852231965 1102 22-23 0.0065020465429554974 1102 24-25 -0.03408486930301535 1102 26-27 -0.05086518903113557 1102 28-29 0.02966248485704881 1102 30-31 0.23017617278667046 1102 32-33 0.3861046407512916 1102 34-35 0.430367649427879 1102 36-37 0.28841375107346323 1102 38-39 0.47469057876026 1102 40-41 0.7252022531459517 1102 42-43 0.8175875030912252 1102 44-45 0.8049880628816055 1102 46-47 1.011120068514991 1102 48-49 1.0410950060897761 1102 50-51 1.1679203540175749 1102 52-53 1.39000934648314 1102 54-55 1.185542161402246 1102 56-57 1.327738713367701 1102 58-59 1.237196403811545 1102 60-61 1.4833331137761618 1102 62-63 1.5165146776406644 1102 64-65 1.3770521403877005 1102 66-67 1.6207964898961684 1102 68-69 1.5839405726937805 1102 70-71 1.3242946357647192 1102 72-73 1.4368308212904068 1102 74-75 1.6081066078546868 1102 76-77 1.3587032453008412 1102 78-79 1.3495308759497568 1102 80-81 1.4344528635499323 1102 82-83 1.440006552225988 1102 84-85 1.5656086261871422 1102 86-87 1.4292477470908693 1102 88-89 1.3592091807865394 1102 90-91 1.9163201770625768 1102 92-93 1.8163264236550134 1102 94-95 1.8368828651921376 1102 96-97 2.0097240920323216 1102 98-99 2.46509660630851 1102 100-101 2.790550780877485 1103 1 0.09398163017374372 1103 2 0.12719566079373124 1103 3 -0.012166020462252902 1103 4 -0.023483545620301527 1103 5 -0.00313591622990117 1103 6 -0.12048626046397004 1103 7 -0.02334084868741826 1103 8 0.03136865535142874 1103 9 0.13499100732610714 1103 10-11 0.0307000284117791 1103 12-13 0.18922515087420066 1103 14-15 0.08812279866400274 1103 16-17 -0.02627856091179126 1103 18-19 -0.055458235793544475 1103 20-21 -0.0657593722501062 1103 22-23 -0.029389504552860046 1103 24-25 0.1032414580746277 1103 26-27 -0.14224735737652594 1103 28-29 -0.06498830347113227 1103 30-31 0.029575594510617975 1103 32-33 -0.01686962153549132 1103 34-35 0.19163343497223906 1103 36-37 0.31807412461196094 1103 38-39 0.17060927213475452 1103 40-41 0.38353853901931245 1103 42-43 0.352495225670566 1103 44-45 0.12163216370662866 1103 46-47 0.19522728367923747 1103 48-49 0.3075836240479717 1103 50-51 0.1284193227375212 1103 52-53 0.14667075130168428 1103 54-55 0.4862860433175129 1103 56-57 0.4687472799516996 1103 58-59 0.33952168725539167 1103 60-61 0.50562030945364 1103 62-63 0.634050551001998 1103 64-65 0.49281648973223113 1103 66-67 0.5294483284850244 1103 68-69 0.7729780024655035 1103 70-71 0.7590748718835592 1103 72-73 0.3095365395168592 1103 74-75 0.2853206965321178 1103 76-77 0.13848620197442685 1103 78-79 0.02863590834333607 1103 80-81 0.24831155920210435 1103 82-83 0.2025725098612554 1103 84-85 0.45336043567238704 1103 86-87 0.4112438434111638 1103 88-89 0.5489038143341425 1103 90-91 0.6837396359885268 1103 92-93 0.7007423901479015 1103 94-95 0.6594797612289867 1103 96-97 0.9614880238033372 1103 98-99 0.8721350926677722 1103 100-101 1.7765272702230348 1104 1 0.011505822622972062 1104 2 0.019394447763886546 1104 3 0.026133011839775122 1104 4 -0.03747512937387398 1104 5 0.002635345866323746 1104 6 0.06920298582197404 1104 7 -0.008058717025974715 1104 8 0.08781628380992856 1104 9 0.14143948763140912 1104 10-11 0.08744580325163298 1104 12-13 -0.04227912539675316 1104 14-15 0.23639951257977287 1104 16-17 0.22615346171325967 1104 18-19 0.16112594370761713 1104 20-21 0.09791234302861795 1104 22-23 0.08069252829435669 1104 24-25 -0.22871046369176895 1104 26-27 0.043174136422031495 1104 28-29 0.006833030180217747 1104 30-31 -6.915370803994847E-6 1104 32-33 0.007365420034631143 1104 34-35 -0.27211409467203396 1104 36-37 -0.28990597624670755 1104 38-39 -0.20872875771395627 1104 40-41 -0.45863128515950535 1104 42-43 -0.2935773346401831 1104 44-45 -0.3615949727251504 1104 46-47 -0.2678749720111071 1104 48-49 -0.33640617082732405 1104 50-51 -0.06566758267340589 1104 52-53 0.20750326118310625 1104 54-55 0.25143400910734925 1104 56-57 0.050486240017121986 1104 58-59 -0.11684033059932375 1104 60-61 0.13578037146809407 1104 62-63 0.031722453687031305 1104 64-65 0.11009449845514396 1104 66-67 0.4219047991133458 1104 68-69 0.460512076337654 1104 70-71 0.40093930898319385 1104 72-73 0.4315401683842417 1104 74-75 0.20179285817856396 1104 76-77 0.3447034058724796 1104 78-79 0.4984784018693027 1104 80-81 0.8364904811047928 1104 82-83 0.29110281857053977 1104 84-85 0.40190037025055503 1104 86-87 0.5181186730418048 1104 88-89 0.5545792436649286 1104 90-91 0.7616623223726045 1104 92-93 0.9103265185382519 1104 94-95 0.6658366680028678 1104 96-97 0.8614471351567516 1104 98-99 1.014676747976484 1104 100-101 1.9370578004124859 1105 1 -0.13685256424641068 1105 2 -0.031590405496707774 1105 3 -0.08310632727411615 1105 4 -0.2108717699267899 1105 5 -0.01744568890298126 1105 6 -0.05433492114277527 1105 7 0.054127158625199456 1105 8 0.10402037222608129 1105 9 0.03027456984100496 1105 10-11 0.04186517824089009 1105 12-13 -0.06924868864570755 1105 14-15 -0.19207899372133852 1105 16-17 -0.05084189053583543 1105 18-19 0.03338729516583783 1105 20-21 -0.01817397562026457 1105 22-23 0.033978620000041815 1105 24-25 -0.08834953230769571 1105 26-27 -0.10469569216708408 1105 28-29 0.10283544861534466 1105 30-31 -0.028675687183998377 1105 32-33 0.09405657669994127 1105 34-35 0.08749202133262202 1105 36-37 0.01948895650131277 1105 38-39 -0.1117721462739496 1105 40-41 -0.26241567765662666 1105 42-43 -0.6015065925473877 1105 44-45 -0.3601608177733766 1105 46-47 -0.6037279861721316 1105 48-49 -0.3033289127138161 1105 50-51 -0.25795323310064333 1105 52-53 -0.1084349139872458 1105 54-55 -0.17466474557048883 1105 56-57 -0.009049099509390146 1105 58-59 -0.134726434912956 1105 60-61 -0.11906796055669133 1105 62-63 0.03478131564710196 1105 64-65 0.1589474610381707 1105 66-67 0.07579922467769507 1105 68-69 0.18842093228030166 1105 70-71 0.2211742181199483 1105 72-73 0.17916758037211622 1105 74-75 0.2636635349392371 1105 76-77 0.20316334409349324 1105 78-79 0.08785591271501048 1105 80-81 0.14469052351336842 1105 82-83 0.01754026878344206 1105 84-85 0.358629293473836 1105 86-87 0.36351934814051745 1105 88-89 0.5810616813558411 1105 90-91 0.9935197607798791 1105 92-93 0.9026614546101825 1105 94-95 0.7993993324077451 1105 96-97 1.0080661972394722 1105 98-99 1.1291111378947036 1105 100-101 1.7249862559952298 1106 1 -0.0399697764758713 1106 2 -0.004677740740795855 1106 3 -0.01344479284077238 1106 4 0.07026645437969847 1106 5 0.13537111701818105 1106 6 -0.17739163130796243 1106 7 -0.11828969779227805 1106 8 -0.05854502751301993 1106 9 -0.22514326377363858 1106 10-11 -0.12491058539895761 1106 12-13 -0.44556545014881266 1106 14-15 -0.18273717576055049 1106 16-17 -0.11283546628263963 1106 18-19 0.02298645730108717 1106 20-21 -0.05261199373347836 1106 22-23 -0.11806437667561909 1106 24-25 -0.07187043450849018 1106 26-27 -0.09029722949929209 1106 28-29 -0.16589143646346116 1106 30-31 -0.054064111371737056 1106 32-33 -0.15321872639483303 1106 34-35 -0.49886368778990686 1106 36-37 -0.164023062088809 1106 38-39 -0.27516278668876737 1106 40-41 -0.46236938936943517 1106 42-43 -0.5644245313626826 1106 44-45 -0.3804458414084593 1106 46-47 -0.052135248290078096 1106 48-49 0.15273228133697359 1106 50-51 0.12781989818509487 1106 52-53 -0.01849069371110801 1106 54-55 0.22441743717940454 1106 56-57 0.08455610348111264 1106 58-59 0.0379312141096122 1106 60-61 0.042744670067456525 1106 62-63 -0.032109934931504824 1106 64-65 0.01079922630512442 1106 66-67 0.13378816224461687 1106 68-69 -0.05167033513040664 1106 70-71 0.13027851638484123 1106 72-73 0.2298929973173678 1106 74-75 0.17750420036843195 1106 76-77 0.02079918151406801 1106 78-79 0.2748395528446146 1106 80-81 0.5393121985882949 1106 82-83 0.3712505789149674 1106 84-85 0.5455918934729027 1106 86-87 0.5585823983983786 1106 88-89 0.7972653872241686 1106 90-91 0.5719269761419774 1106 92-93 0.6055537712220698 1106 94-95 0.4596316154489344 1106 96-97 0.8425621926012923 1106 98-99 0.9115772282176451 1106 100-101 1.1094876282793003 1107 1 -0.1330494710639556 1107 2 -0.17727778678904826 1107 3 -0.06881470342004548 1107 4 -0.11676411792538488 1107 5 -0.18448376876349215 1107 6 -0.2226495948551701 1107 7 -0.15868148129082726 1107 8 -0.07840800285231353 1107 9 -0.3338482500525046 1107 10-11 -0.15244619500935386 1107 12-13 -0.22114556477162495 1107 14-15 -0.3761709440631691 1107 16-17 -0.25036416865536637 1107 18-19 -0.167920879927685 1107 20-21 -0.062051885208951774 1107 22-23 -0.09951439876633827 1107 24-25 -0.3426652646588906 1107 26-27 -0.35964527338203567 1107 28-29 -0.11523524888529124 1107 30-31 -0.2386709277574468 1107 32-33 -0.009616633066222846 1107 34-35 -0.0750891002017795 1107 36-37 0.08767107796010265 1107 38-39 -0.21247182645828389 1107 40-41 -0.09189254106108535 1107 42-43 0.14042795866537716 1107 44-45 0.12978433761967523 1107 46-47 0.05385351344755662 1107 48-49 0.5268774328917161 1107 50-51 0.45846500929205547 1107 52-53 0.4619823587279015 1107 54-55 0.6706405471787207 1107 56-57 0.7259242343577128 1107 58-59 0.7926433814265508 1107 60-61 0.9699355400682563 1107 62-63 1.084737502234411 1107 64-65 1.2556794697385776 1107 66-67 1.3868233020383904 1107 68-69 1.2962708724538636 1107 70-71 1.1533990943526184 1107 72-73 1.1796257307788913 1107 74-75 1.096098359707824 1107 76-77 1.0739382410096532 1107 78-79 1.0887143887199393 1107 80-81 0.8054990922603942 1107 82-83 1.069491411268217 1107 84-85 1.1451712364763686 1107 86-87 0.9745785651926084 1107 88-89 1.7186068847879632 1107 90-91 1.2639886310165593 1107 92-93 1.026733477633357 1107 94-95 0.881414927313827 1107 96-97 1.046857747700514 1107 98-99 1.421182022213948 1107 100-101 1.5136543727301763 1108 1 0.024165576662042554 1108 2 0.05159644117277651 1108 3 0.017934313985904282 1108 4 -0.15590411418217087 1108 5 -0.0350826832310176 1108 6 -0.12090432066464274 1108 7 -0.03616136150792926 1108 8 -0.023780452786809292 1108 9 0.019815422041823183 1108 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2316 6 0.21480602162464635 2316 7 -0.3289628582567943 2316 8 -0.3451980702570552 2316 9 -0.1597484351536096 2316 10-11 -0.06756162126577436 2316 12-13 0.03576080200797094 2316 14-15 0.06349075164923335 2316 16-17 -0.025413936405868753 2316 18-19 0.10007991169969443 2316 20-21 0.22301546132593586 2316 22-23 0.3853864731879426 2316 24-25 0.42142977719257857 2316 26-27 0.6342894639257395 2316 28-29 0.1599018307081863 2316 30-31 -0.009576293361234889 2316 32-33 -0.004348818539860133 2316 34-35 0.08075671872509815 2316 36-37 0.06989438672970039 2316 38-39 0.14579194844014864 2316 40-41 -0.024668242757257985 2316 42-43 -0.426476196214189 2316 44-45 -0.33911518391142437 2316 46-47 -0.5768173907538809 2316 48-49 -0.8773647585191284 2316 50-51 -0.8417771355524692 2316 52-53 -0.8810134852283724 2316 54-55 -0.4577311451576307 2316 56-57 -0.37683598403831553 2316 58-59 -0.9759394891540083 2316 60-61 -0.6138896499805142 2316 62-63 -0.3420195030857869 2316 64-65 -0.5926773213765664 2316 66-67 -0.5914593322181183 2316 68-69 -0.39561727525449086 2316 70-71 -0.5200720752768326 2316 72-73 -0.09209454535936246 2316 74-75 -0.010218361092181993 2316 76-77 -0.22224099274161446 2316 78-79 -0.059721565132843324 2316 80-81 -0.027269624488344135 2316 82-83 -0.0602830913453154 2316 84-85 -0.09884208149008344 2316 86-87 0.377877788699287 2316 88-89 0.675928855940132 2316 90-91 0.5461863283122206 2316 92-93 0.25457664228020604 2316 94-95 0.7925896790571514 2316 96-97 0.5888023570673298 2316 98-99 1.2102715343153712 2316 100-101 0.821812791338072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 18.0 6 30.0 7 91.0 8 183.0 9 327.0 10 576.0 11 966.0 12 1529.0 13 2092.0 14 2704.0 15 3812.0 16 5477.0 17 7774.0 18 6973.0 19 3929.0 20 3406.0 21 3454.0 22 3429.0 23 3640.0 24 4138.0 25 4706.0 26 5728.0 27 6683.0 28 8150.0 29 9849.0 30 11996.0 31 14830.0 32 18864.0 33 24422.0 34 32669.0 35 46398.0 36 66887.0 37 89617.0 38 59318.0 39 4089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.683269364968595 19.807440683879975 15.927468597348222 20.58182135380321 2 15.844048836099129 21.709437852990348 39.411542879812714 23.034970431097808 3 19.35068303837091 26.073166956942533 30.84059386300868 23.735556141677876 4 12.804980392755239 18.007494130905332 36.90412613158543 32.283399344754 5 13.149691775148446 38.3853290202199 33.09973057573089 15.365248628900765 6 30.294991487905413 37.595774687799036 16.708555036522444 15.400678787773101 7 27.204479912110717 34.99201105591389 20.2701200412417 17.533388990733698 8 27.126007337185754 34.138185844855364 19.085184159874792 19.650622658084092 9 27.07761591598203 16.81841664141739 20.342271214297703 35.76169622830288 10-11 25.12877131565811 27.52839726305097 26.05747244195754 21.285358979333374 12-13 26.693690875460106 25.205869754068072 27.38451946647715 20.715919903994664 14-15 23.051645853269363 25.9654851457955 27.307366177012334 23.675502823922802 16-17 21.039260265193708 29.513862398339867 27.663326496046942 21.78355084041948 18-19 21.00667234866237 26.76339864722 31.883189212636697 20.346739791480932 20-21 21.986295621770495 25.619143841476504 32.193408950460864 20.201151586292134 22-23 21.512050562991753 25.609449062625 32.450580866236734 20.427919508146513 24-25 21.63194618525195 25.863470798393056 32.323834518777836 20.18074849757716 26-27 21.093692949404737 26.159649568336462 32.459397687403886 20.287259794854915 28-29 20.67194925229978 26.616057025766228 32.33509177311767 20.37690194881632 30-31 21.019360970271908 26.013606302233423 32.31583972377594 20.65119300371874 32-33 20.886152518154976 26.27831418927388 32.202673083986454 20.63286020858469 34-35 21.438925448874027 26.13736188281865 31.840029296427975 20.583683371879353 36-37 21.596306557473532 25.89921941585887 31.958937045376768 20.54553698129083 38-39 21.302361370567116 26.357194082295933 31.765589339936657 20.574855207200297 40-41 21.050159233933286 26.42182496692163 31.810275111768927 20.71774068737616 42-43 21.175724240769384 26.585743109125808 31.10150434539011 21.137028304714693 44-45 21.34334149302793 26.733535472873555 30.862827759237422 21.060295274861094 46-47 21.768728242933655 27.205242839922487 30.096085308408117 20.92994360873574 48-49 22.010794338639677 27.25984667330777 29.589065282642956 21.140293705409594 50-51 21.76676642856053 27.5457266233469 29.411412092187838 21.276094855904734 52-53 22.034663080179353 28.007188959780628 28.788100069971378 21.17004789006864 54-55 21.55369158970178 27.76904649282085 28.92466414827829 21.752597769199078 56-57 21.995099823654684 27.903757746442032 28.68227108351008 21.418871346393203 58-59 22.048504770478615 27.45068761593778 28.953873384500355 21.546934229083245 60-61 21.7778833766749 27.53079503617368 29.66306928038469 21.028252306766735 62-63 21.352932585518758 27.76239812188971 29.542199717062772 21.342469575528764 64-65 21.41440276920998 27.582020189249697 29.410431185001272 21.593145856539053 66-67 21.44121423230934 27.598259652671665 29.10929267872674 21.851233436292258 68-69 21.367428213942397 27.53777037616701 29.26754570482541 21.827255705065188 70-71 21.590639093728953 27.652754496369553 28.901776313925176 21.854830095976318 72-73 21.629401154204565 27.47573609150804 28.999634883353405 21.89522787093399 74-75 21.28154434027452 28.155850893388468 28.387018020354915 22.175586745982095 76-77 21.404702687031754 27.595752889861565 28.88150423206956 22.118040191037124 78-79 21.48818878757692 27.751935111899712 28.618185147321363 22.14169095320201 80-81 21.444047964181628 28.04936796880279 28.413284535017297 22.09329953199828 82-83 21.5505778645822 27.86828785547064 28.399940709480813 22.181193570466345 84-85 21.25717698655936 27.82000531870834 28.314165638528376 22.608652056203923 86-87 21.141165622908762 27.597714704234683 28.28761942544996 22.97350024740659 88-89 21.032611894262565 27.359136278525327 28.207947963963647 23.40030386324846 90-91 21.197188241539163 27.433392956215876 28.428124996593755 22.941293805651203 92-93 21.027896305742203 27.2390886153209 28.619789537931673 23.113225541005225 94-95 20.996209338672376 27.391397225994474 28.234432458000825 23.377960977332325 96-97 21.16209164288875 27.53864229366617 28.048823020365816 23.250443043079265 98-99 21.202028084523867 27.807035517636752 27.31756176459048 23.673374633248905 100-101 21.140952399638547 27.17195928969522 27.620723145292885 24.066365165373348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 547.0 1 375.5 2 239.0 3 301.5 4 283.5 5 266.5 6 446.0 7 908.5 8 1374.0 9 1596.0 10 1476.5 11 1252.0 12 1235.5 13 1312.0 14 1383.0 15 1463.0 16 1527.0 17 1551.5 18 1573.5 19 1610.0 20 1750.5 21 1864.0 22 2006.0 23 2280.0 24 2639.5 25 3074.0 26 3553.5 27 4140.0 28 4753.0 29 5380.5 30 6114.0 31 6795.5 32 7512.0 33 8136.0 34 8797.0 35 9344.0 36 10078.5 37 11062.0 38 11688.0 39 11825.5 40 11943.5 41 12694.5 42 13820.0 43 14779.5 44 15110.5 45 15540.0 46 15649.5 47 16305.5 48 18460.0 49 18498.0 50 16557.0 51 15378.5 52 14769.5 53 14451.0 54 14149.0 55 13221.5 56 11653.5 57 10329.0 58 9547.0 59 8500.0 60 7164.5 61 5973.0 62 5044.0 63 4008.5 64 2908.5 65 2205.5 66 1650.5 67 1401.0 68 1072.0 69 643.5 70 487.0 71 370.5 72 278.5 73 244.0 74 220.5 75 175.0 76 119.0 77 80.5 78 44.0 79 25.5 80 19.0 81 9.0 82 3.0 83 3.5 84 2.5 85 2.0 86 2.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043377895583519885 2 0.0 3 0.0 4 0.0 5 6.539381243746717E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007520288430308724 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.089896873957786E-4 22-23 9.809071865620074E-4 24-25 0.0 26-27 0.0022887834353113507 28-29 0.004141608121039588 30-31 2.179793747915572E-4 32-33 1.089896873957786E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01187987592613987 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.269690621873358E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.179793747915572E-4 84-85 2.179793747915572E-4 86-87 0.0 88-89 0.0 90-91 0.00926412342864118 92-93 0.001416865936145122 94-95 0.0 96-97 0.0 98-99 2.179793747915572E-4 100-101 0.01187987592613987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 458759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.74058288247879 #Duplication Level Percentage of deduplicated Percentage of total 1 81.64551888022888 50.408419254077586 2 10.800866051097415 13.337035312606629 3 3.1889865679052978 5.906696685206059 4 1.4304815804063624 3.5327506630775303 5 0.7738831402603347 2.388999808129808 6 0.4625919329481351 1.7136417346170245 7 0.3134449853123334 1.3546593266341427 8 0.2153235746333125 1.063536240495971 9 0.1614076347888184 0.8968861308189517 >10 0.9187800492499598 10.185383550223419 >50 0.055844881715489694 2.4303299178243103 >100 0.030043132511405536 3.435456931071542 >500 0.0014137944711249662 0.700490170154332 >1k 0.0014137944711249662 2.6457142750626548 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4573 0.9968196809217912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3673 0.8006382436093896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2667 0.5813509925690831 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1211 0.2639730228725758 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 978 0.21318382854614298 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.20359273605531444 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.14691809860950955 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 624 0.1360191298699317 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 492 0.10724585239744616 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.1797937479155723E-4 0.0 8 0.0 0.0 0.0 2.1797937479155723E-4 0.0 9 0.0 0.0 0.0 2.1797937479155723E-4 0.0 10-11 0.0 0.0 0.0 2.1797937479155723E-4 0.0 12-13 0.0 0.0 0.0 2.1797937479155723E-4 0.0 14-15 0.0 0.0 0.0 3.269690621873358E-4 0.0 16-17 0.0 0.0 0.0 5.44948436978893E-4 0.0 18-19 0.0 0.0 0.0 6.539381243746717E-4 0.0 20-21 0.0 0.0 0.0 6.539381243746717E-4 0.0 22-23 0.0 0.0 0.0 6.539381243746717E-4 0.0 24-25 0.0 0.0 0.0 8.719174991662289E-4 0.0 26-27 0.0 0.0 0.0 0.0011988865613535647 0.0 28-29 0.0 0.0 0.0 0.0027247421848944652 0.0 30-31 0.0 0.0 0.0 0.005558474057184709 0.0 32-33 0.0 0.0 0.0 0.009700082178224295 0.0 34-35 0.0 0.0 0.0 0.015476535610200563 0.0 36-37 0.0 0.0 0.0 0.0188552159194697 0.0 38-39 0.0 0.0 0.0 0.02452267966405019 0.0 40-41 0.0 0.0 0.0 0.03149801965738002 0.0 42-43 0.0 0.0 0.0 0.04730152432976792 0.0 44-45 0.0 0.0 0.0 0.06964441024590254 0.0 46-47 0.0 0.0 0.0 0.09144234772505826 0.0 48-49 0.0 0.0 0.0 0.1488799129826336 0.0 50-51 0.0 0.0 0.0 0.21590857073103742 0.0 52-53 0.0 0.0 0.0 0.2820653109802751 0.0 54-55 0.0 0.0 0.0 0.39628650337105104 0.0 56-57 0.0 0.0 0.0 0.5209707057518218 0.0 58-59 0.0 0.0 0.0 0.676934948415181 0.0 60-61 0.0 0.0 0.0 0.8613454994888383 0.0 62-63 0.0 0.0 0.0 1.0913137398939312 0.0 64-65 0.0 0.0 0.0 1.284879424708834 0.0 66-67 0.0 0.0 0.0 1.5454737672721408 0.0 68-69 0.0 0.0 0.0 1.9090633644244581 0.0 70-71 0.0 0.0 0.0 2.2120546953847224 0.0 72-73 0.0 0.0 0.0 2.5985321268901536 0.0 74-75 0.0 0.0 0.0 3.059776483949089 0.0 76-77 0.0 0.0 0.0 3.4718664919925275 0.0 78-79 0.0 0.0 0.0 3.886245283471278 0.0 80-81 0.0 0.0 0.0 4.403946298601227 0.0 82-83 0.0 0.0 0.0 5.080227308892033 0.0 84-85 0.0 0.0 0.0 5.787243411028449 0.0 86-87 0.0 0.0 0.0 6.528373285319743 0.0 88-89 0.0 0.0 0.0 7.497836554705193 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGTA 40 5.3858705E-4 47.49804 2 GGACCAT 110 6.999471E-8 34.54403 6 GGTATCA 3290 0.0 32.786495 1 ACGCGAA 30 0.003951339 31.66536 78-79 GTATCAA 4715 0.0 27.916637 1 GTACAAC 70 0.008388097 27.141735 6 AGTGTCG 70 0.008388097 27.141735 5 AGCGCTC 110 9.467124E-5 25.90802 8 AGTATAC 100 0.0016525337 23.74902 5 GTATATA 315 0.0 22.627977 1 ATCAACG 5850 0.0 22.571716 3 TATCAAC 5815 0.0 22.54421 2 TCAACGC 5890 0.0 22.41843 4 GAGTACT 3530 0.0 22.336187 12-13 CAACGCA 5940 0.0 22.229723 5 CCATATA 150 3.0290556E-5 22.16575 2 GTACATG 2590 0.0 22.01641 1 AACGCAG 6030 0.0 21.897936 6 GTGTAGG 175 4.026666E-6 21.72286 1 AGGTAAG 175 4.0419236E-6 21.713388 2 >>END_MODULE