FastQCFastQC Report
Thu 26 May 2016
SRR937514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416439
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36030.8651927413138539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.6253016648296629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.45048614562997225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8540.20507205136886794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8230.19762798393041955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.1690523702150855No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6070.14575964306897288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5820.1397563628766758No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4740.11382219244595246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.11238140519980117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.036.9516941
TAGAGTG1151.0236727E-733.080115
GGTATCA22350.032.7734871
CAACGCA45800.029.6944565
TATCAAC46000.029.665152
ATCAACG46250.029.5047933
TCAACGC46550.029.3181784
AACGCAG46850.029.0988856
GTACATG23750.028.2380681
ACTCGAG605.3783133E-627.70900238-39
TTAGGAC700.0083452127.1696723
TACATGG24900.026.1603572
CGCAGAG51600.026.1440418
ACGCAGA51750.026.068267
CGCGGAA559.4874944E-525.90347982-83
ACATGGG24300.024.849633
CTAGGTA1151.2702344E-424.8100814
GCAGAGT53050.024.8026719
GAGTACT28300.024.67076912-13
AGTACTT28450.024.54069312-13
Produced by FastQC (version 0.11.5)