##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937514_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 416439 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65513796738538 33.0 31.0 34.0 30.0 34.0 2 31.734645410252163 33.0 31.0 34.0 30.0 34.0 3 31.75713129654043 33.0 31.0 34.0 30.0 34.0 4 35.4085976577602 37.0 35.0 37.0 33.0 37.0 5 35.40830950031097 37.0 35.0 37.0 33.0 37.0 6 35.460427577628415 37.0 35.0 37.0 33.0 37.0 7 35.402431088346674 37.0 35.0 37.0 33.0 37.0 8 35.40338200792913 37.0 35.0 37.0 33.0 37.0 9 37.007859494427755 39.0 37.0 39.0 33.0 39.0 10-11 36.882906019849244 39.0 37.0 39.0 33.0 39.0 12-13 36.73033025244994 39.0 37.0 39.0 32.5 39.0 14-15 37.84068254894474 40.0 38.0 41.0 33.0 41.0 16-17 37.84301542362747 40.0 38.0 41.0 33.0 41.0 18-19 37.855749581571374 40.0 38.0 41.0 33.0 41.0 20-21 37.820943763672474 40.0 38.0 41.0 33.0 41.0 22-23 37.675900912258456 40.0 37.0 41.0 32.0 41.0 24-25 37.49752904987285 40.0 37.0 41.0 32.0 41.0 26-27 37.3358174906769 40.0 37.0 41.0 32.0 41.0 28-29 37.3490199044758 40.0 37.0 41.0 32.0 41.0 30-31 37.11980986410975 39.0 36.5 41.0 31.5 41.0 32-33 36.839849293654055 39.0 35.5 41.0 31.0 41.0 34-35 36.673804566815306 39.0 35.0 41.0 31.0 41.0 36-37 36.63965190580133 39.0 35.0 41.0 30.5 41.0 38-39 36.5254587106395 39.0 35.0 41.0 30.0 41.0 40-41 36.28937371379722 39.0 35.0 41.0 30.0 41.0 42-43 35.987004099039716 39.0 35.0 41.0 29.5 41.0 44-45 35.64359125826351 39.0 35.0 41.0 27.5 41.0 46-47 35.13881624919856 39.0 35.0 40.5 25.0 41.0 48-49 34.711054920408515 39.0 35.0 40.0 23.5 41.0 50-51 33.74585473502722 37.5 33.5 39.5 22.0 40.5 52-53 33.786985608936725 38.0 33.5 39.5 21.5 40.5 54-55 34.072717732969295 38.0 34.0 40.0 20.5 41.0 56-57 33.854796260676835 38.0 34.0 40.0 19.0 41.0 58-59 33.59252375497972 38.0 33.5 40.0 18.0 41.0 60-61 33.2853335062278 37.5 33.0 40.0 14.0 41.0 62-63 32.928892346778284 37.0 33.0 40.0 10.5 41.0 64-65 32.53929747213878 36.0 32.0 39.5 7.5 41.0 66-67 32.146458424883356 36.0 32.0 39.0 4.5 41.0 68-69 31.67117032746693 35.0 31.0 39.0 2.0 40.0 70-71 31.21429909302443 35.0 31.0 38.0 2.0 40.0 72-73 30.82301729665089 35.0 31.0 37.5 2.0 40.0 74-75 30.367900220680582 35.0 30.0 37.0 2.0 39.0 76-77 29.966428456508638 35.0 30.0 36.5 2.0 39.0 78-79 29.50487706482822 34.0 29.0 36.0 2.0 38.5 80-81 29.0331513138779 34.0 29.0 35.5 2.0 37.0 82-83 28.559667802487276 34.0 29.0 35.0 2.0 37.0 84-85 28.127981529107505 34.0 27.5 35.0 2.0 36.5 86-87 27.714862200706467 34.0 26.5 35.0 2.0 36.0 88-89 27.30164081654216 34.0 26.0 35.0 2.0 36.0 90-91 26.902725249076095 33.0 25.5 35.0 2.0 35.0 92-93 26.460180242484494 33.0 25.0 35.0 2.0 35.0 94-95 25.923812371079556 33.0 23.5 35.0 2.0 35.0 96-97 25.29726682659405 32.0 19.5 35.0 2.0 35.0 98-99 24.553668124263098 32.0 9.5 34.5 2.0 35.0 100-101 22.77789904403766 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03688554513535536 1101 2 0.14606732843841286 1101 3 0.19805644379275478 1101 4 0.06890152658815651 1101 5 -0.032762602760662674 1101 6 0.007156462590053536 1101 7 0.13780059804570044 1101 8 0.0891106867866327 1101 9 0.21462939935248215 1101 10-11 0.151438822696619 1101 12-13 0.08177856301058029 1101 14-15 0.12068600338229629 1101 16-17 0.35963012339125555 1101 18-19 0.30088418253327376 1101 20-21 0.20156295981424677 1101 22-23 0.14597379496407115 1101 24-25 0.09808964147674004 1101 26-27 0.016311099347213087 1101 28-29 0.06251893382187745 1101 30-31 0.15474014888877008 1101 32-33 0.1762873425247946 1101 34-35 0.15214160693037826 1101 36-37 0.3779825995510606 1101 38-39 0.4419211997051775 1101 40-41 0.25762917692750875 1101 42-43 0.4612788674722381 1101 44-45 0.5885823896608358 1101 46-47 0.703570891872225 1101 48-49 0.867897488822102 1101 50-51 0.725040330285232 1101 52-53 0.6944868320842446 1101 54-55 0.7559242028113715 1101 56-57 0.7416210841945485 1101 58-59 0.7438382425481009 1101 60-61 0.7851075584876526 1101 62-63 0.6637483577367647 1101 64-65 0.9707741014547011 1101 66-67 0.8594995285695965 1101 68-69 0.977858023541355 1101 70-71 1.1757886773289243 1101 72-73 0.9651519720558532 1101 74-75 1.1450391834596978 1101 76-77 1.1322053224035393 1101 78-79 0.7981713933207573 1101 80-81 1.028253021002886 1101 82-83 1.04665129690569 1101 84-85 1.1584479810246755 1101 86-87 1.2272125620887735 1101 88-89 1.2331362643591852 1101 90-91 1.4588725918596168 1101 92-93 1.7277018267388975 1101 94-95 1.8445328212405592 1101 96-97 1.914763723993186 1101 98-99 1.9740571689873363 1101 100-101 2.182226877494358 1102 1 0.02688737966112953 1102 2 -0.06215134703397496 1102 3 -0.02315296732447436 1102 4 -0.07121037948412834 1102 5 -0.16339230373296232 1102 6 -0.09607000961688783 1102 7 0.029941948256670514 1102 8 -0.1560966146259517 1102 9 -0.028858034145962108 1102 10-11 0.20011108466690075 1102 12-13 0.07986950962588679 1102 14-15 -0.04064907275052576 1102 16-17 0.24658893828760853 1102 18-19 0.3514425663160594 1102 20-21 0.08063904677077005 1102 22-23 0.0486980657400764 1102 24-25 0.21050594123825306 1102 26-27 -0.09341074068213828 1102 28-29 0.10317661131223588 1102 30-31 -0.0419668773449402 1102 32-33 0.11126486958405479 1102 34-35 0.04031579515272199 1102 36-37 0.2193649147225898 1102 38-39 0.4752866372395701 1102 40-41 0.5645166746343477 1102 42-43 0.573173473966456 1102 44-45 0.23694392882795512 1102 46-47 0.4864719650698035 1102 48-49 0.6475537445549904 1102 50-51 0.6589171418794351 1102 52-53 0.7220047187105507 1102 54-55 0.5672546926297528 1102 56-57 0.5795923280030237 1102 58-59 0.8748291157823687 1102 60-61 1.1120372502873295 1102 62-63 0.7814503848877408 1102 64-65 0.9451423366408989 1102 66-67 0.810918992888066 1102 68-69 0.7855710578469939 1102 70-71 0.8806765419409217 1102 72-73 0.9354154558203014 1102 74-75 1.1250772498695305 1102 76-77 1.1129600004442644 1102 78-79 1.2196250256817898 1102 80-81 1.1185460864954564 1102 82-83 1.1058606162966278 1102 84-85 1.2066673444442308 1102 86-87 1.186658076673254 1102 88-89 1.1339629475365847 1102 90-91 1.1929924781190202 1102 92-93 1.2323110727488 1102 94-95 1.121592076423095 1102 96-97 1.3580539459708056 1102 98-99 1.3644958499633049 1102 100-101 1.7138667142215631 1103 1 -0.12021522690852748 1103 2 -0.1273648806906671 1103 3 -0.08090366727320841 1103 4 -0.1349451482052899 1103 5 -0.026438953837462975 1103 6 -0.02996428778242688 1103 7 0.018057607391497754 1103 8 -0.0697170255254349 1103 9 -0.009077088525074828 1103 10-11 0.04578224677896969 1103 12-13 0.02198325622472197 1103 14-15 0.052700428412769895 1103 16-17 -0.008064242042848946 1103 18-19 0.04783240818679957 1103 20-21 -0.2676864984005718 1103 22-23 -0.33519916995533805 1103 24-25 -0.2592557804384725 1103 26-27 -0.01812188859806696 1103 28-29 -0.04957980652758209 1103 30-31 -0.004502367703402399 1103 32-33 -0.16509657999991845 1103 34-35 -0.03722077963335124 1103 36-37 0.07089198597933688 1103 38-39 0.19071064843875263 1103 40-41 0.23646992594551364 1103 42-43 0.049860744757886266 1103 44-45 0.2586817733801894 1103 46-47 0.46717901187764 1103 48-49 0.541560803192958 1103 50-51 0.41767882412242585 1103 52-53 0.24014916446945023 1103 54-55 0.3214124867068122 1103 56-57 0.4570865115276064 1103 58-59 0.41387836851315996 1103 60-61 0.3729825754184475 1103 62-63 0.3298774719179889 1103 64-65 0.3859720223540677 1103 66-67 0.4746034836014239 1103 68-69 0.6869744057708083 1103 70-71 0.6470021068291274 1103 72-73 0.5664192414585365 1103 74-75 0.44234380217837455 1103 76-77 0.7111154876480192 1103 78-79 0.6710254160757323 1103 80-81 0.7924833474284583 1103 82-83 0.767598066511539 1103 84-85 0.6572839894223428 1103 86-87 1.000153778396509 1103 88-89 0.8706269524263668 1103 90-91 1.19956065904098 1103 92-93 0.9828064590022016 1103 94-95 1.0228648001109377 1103 96-97 0.9835789075229151 1103 98-99 0.9214997736195976 1103 100-101 1.3720083010349242 1104 1 -0.002733341676439238 1104 2 0.11937834976547634 1104 3 0.17773568357646852 1104 4 0.1085153881733163 1104 5 0.13377466432007878 1104 6 0.11096633816543999 1104 7 -0.14453296644806102 1104 8 0.006925617740016321 1104 9 -0.08660278621908901 1104 10-11 0.008311573907697323 1104 12-13 0.11449090396216377 1104 14-15 0.16007498607541493 1104 16-17 0.08063330938494317 1104 18-19 0.12432598538357098 1104 20-21 0.05578930104723412 1104 22-23 0.03482331957763307 1104 24-25 0.01493910076235494 1104 26-27 -0.0609297748412061 1104 28-29 -0.09092133633431843 1104 30-31 0.04981844191978979 1104 32-33 -0.032931687003433296 1104 34-35 0.19948803600433251 1104 36-37 0.2855536060034467 1104 38-39 0.3620904493383321 1104 40-41 0.5952873459359296 1104 42-43 0.7438159536448197 1104 44-45 0.9487410109396563 1104 46-47 0.8275916008311981 1104 48-49 0.6893857348169945 1104 50-51 0.8378353110888597 1104 52-53 0.8643746690256933 1104 54-55 1.1753836391819519 1104 56-57 1.1382163675168258 1104 58-59 1.0696783348112646 1104 60-61 1.1944832494563258 1104 62-63 1.011406727754256 1104 64-65 1.1877627595181792 1104 66-67 1.2155104534123033 1104 68-69 1.2031521249303374 1104 70-71 1.2594643542269495 1104 72-73 1.1800124466382584 1104 74-75 1.2150820486429161 1104 76-77 1.319177045453248 1104 78-79 1.3811008240630827 1104 80-81 1.39594005993834 1104 82-83 1.2640350634135586 1104 84-85 1.4175492946189188 1104 86-87 1.1321817574494872 1104 88-89 1.1169448585017818 1104 90-91 1.3505978024166119 1104 92-93 1.2327521795098058 1104 94-95 1.5675473541959946 1104 96-97 1.2758237453001477 1104 98-99 1.2114859363997468 1104 100-101 1.3833446135292533 1105 1 -0.12107818656663127 1105 2 -0.01686690796097423 1105 3 0.08156365375561236 1105 4 -0.04112323490044645 1105 5 -0.09259528064282563 1105 6 0.12936624337805114 1105 7 0.0694379514795358 1105 8 0.06861873427043008 1105 9 0.17872524087895414 1105 10-11 -0.13150215028422707 1105 12-13 -0.15106363712050808 1105 14-15 -0.0362626893567608 1105 16-17 -0.16805813675002668 1105 18-19 -0.060681344376639856 1105 20-21 -0.01693214250895636 1105 22-23 0.049522334764525056 1105 24-25 0.1594039121052475 1105 26-27 0.08133959326608675 1105 28-29 -0.05156598351845787 1105 30-31 -0.0312805342370055 1105 32-33 -0.008736508614958893 1105 34-35 0.08445261851001362 1105 36-37 0.06629736176793699 1105 38-39 0.21524433713143054 1105 40-41 -0.07671691949664705 1105 42-43 0.03727934085363671 1105 44-45 0.04959897621679943 1105 46-47 0.45816587767078687 1105 48-49 0.5837616829812973 1105 50-51 0.21586856566502632 1105 52-53 0.48516121850912697 1105 54-55 0.32997652966302127 1105 56-57 0.3662683240168505 1105 58-59 0.5335499350686703 1105 60-61 0.5786927853465897 1105 62-63 0.6583295062328673 1105 64-65 0.8315840388226974 1105 66-67 0.608994284960243 1105 68-69 0.6536350062811458 1105 70-71 0.8696907055861516 1105 72-73 1.0095519611316703 1105 74-75 0.7790379817992239 1105 76-77 0.700597555307187 1105 78-79 0.749383522808774 1105 80-81 1.013500817958679 1105 82-83 1.1912055901108474 1105 84-85 1.2393757175333064 1105 86-87 1.2708501340063307 1105 88-89 1.2634076393500813 1105 90-91 1.2146487552854417 1105 92-93 1.3601575837849929 1105 94-95 1.161316012559471 1105 96-97 1.166065522842505 1105 98-99 1.328649423739357 1105 100-101 1.545262617790037 1106 1 -0.0013198202670494652 1106 2 0.03624617518525497 1106 3 -0.32897485438794405 1106 4 0.010421723936289595 1106 5 -0.02701560923615176 1106 6 -0.23204720685182423 1106 7 -0.12895472110323425 1106 8 -0.08676812912455745 1106 9 -0.18959508415044724 1106 10-11 -0.3773038538374536 1106 12-13 -0.3909492353242925 1106 14-15 -0.5612635803364796 1106 16-17 -0.35072679921127303 1106 18-19 -0.10025866359685409 1106 20-21 -0.2381552794703623 1106 22-23 -0.34449028150833527 1106 24-25 -0.3220441081378169 1106 26-27 -0.43717140142367583 1106 28-29 0.10951245833621215 1106 30-31 -0.2987134434639884 1106 32-33 -0.1547217539907777 1106 34-35 -0.22778053306345925 1106 36-37 -0.020398529033585078 1106 38-39 -0.25610531895038946 1106 40-41 -0.03606686069198872 1106 42-43 -0.2136633156914982 1106 44-45 -0.2366353870890876 1106 46-47 -0.3432703816399538 1106 48-49 -0.07917890649088122 1106 50-51 -0.3517211559929052 1106 52-53 -0.28493037420622613 1106 54-55 -0.5637759205312136 1106 56-57 -0.8351037847539544 1106 58-59 -0.902606022021736 1106 60-61 -0.6332338692027548 1106 62-63 -0.5402930391180014 1106 64-65 -0.3571379398650656 1106 66-67 -0.30394095863361414 1106 68-69 -0.43555740969946655 1106 70-71 -0.1153711284065082 1106 72-73 -0.13871597429011828 1106 74-75 -0.28078952452673533 1106 76-77 -0.14379105440805517 1106 78-79 -0.2464805493249429 1106 80-81 -0.10315693891077515 1106 82-83 -0.377793661466189 1106 84-85 -0.5320951117906212 1106 86-87 -0.4916164542541175 1106 88-89 -0.34091361229823036 1106 90-91 -0.48222746981167575 1106 92-93 -0.16221491701688606 1106 94-95 0.3398612208705316 1106 96-97 0.4595756265525459 1106 98-99 0.09710874160528249 1106 100-101 0.13774453090169558 1107 1 0.023810936854768983 1107 2 0.06736890915163585 1107 3 -0.031503671734210315 1107 4 -0.1361328893780822 1107 5 -0.2992290861061022 1107 6 -0.03312880945173191 1107 7 0.033534488260499984 1107 8 0.25061063490326774 1107 9 0.1784493986607032 1107 10-11 0.18881862623160117 1107 12-13 0.2915851677631025 1107 14-15 0.1045567619171095 1107 16-17 0.06782293927448535 1107 18-19 -0.06829299051505444 1107 20-21 -0.015025233261049209 1107 22-23 0.14137779344215318 1107 24-25 -0.06435609716945123 1107 26-27 -0.19049491739381352 1107 28-29 -0.11040832569872805 1107 30-31 -0.13259858031464233 1107 32-33 -0.19034862648353368 1107 34-35 -0.21149809768032668 1107 36-37 -0.2714674367673027 1107 38-39 -0.23040075387650205 1107 40-41 -0.2792090939728453 1107 42-43 -0.37430923048356135 1107 44-45 -0.06438382562054557 1107 46-47 0.04623350298910367 1107 48-49 0.12232162669349833 1107 50-51 -0.17163178413488112 1107 52-53 -0.3047089757397643 1107 54-55 -0.1532950389997083 1107 56-57 -0.27058339679884114 1107 58-59 0.05490172184664033 1107 60-61 0.016268492042357252 1107 62-63 0.14954852895187543 1107 64-65 0.3103301960207858 1107 66-67 0.3933296451342194 1107 68-69 0.30605382271814463 1107 70-71 0.16284702017420827 1107 72-73 0.20641935298353076 1107 74-75 -0.2318178086886178 1107 76-77 -0.2530989303030431 1107 78-79 -0.10276704706525663 1107 80-81 -0.026754156196105328 1107 82-83 0.07769052073053118 1107 84-85 -0.07869034867682245 1107 86-87 0.06410639142708874 1107 88-89 -0.03313673726649213 1107 90-91 0.37754757715932996 1107 92-93 0.36163873656560597 1107 94-95 0.3789055513432871 1107 96-97 0.544113279525682 1107 98-99 0.5937877646083791 1107 100-101 0.647334232826239 1108 1 -0.45357182358288384 1108 2 -0.02678228571554797 1108 3 -0.30369162697705576 1108 4 -0.1506448690451947 1108 5 -0.32856299801758126 1108 6 -0.5379051786672449 1108 7 -0.5389851162400134 1108 8 -0.6713495051952947 1108 9 -0.4821897235535957 1108 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-1.653922377198338 2316 88-89 -2.420068298899725 2316 90-91 -2.4027852616460628 2316 92-93 -2.256493237890954 2316 94-95 -3.048005369123718 2316 96-97 -2.341689468329573 2316 98-99 -2.1320459449962144 2316 100-101 -3.0157632321805323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1737.0 3 381.0 4 301.0 5 372.0 6 421.0 7 507.0 8 571.0 9 761.0 10 988.0 11 1272.0 12 1718.0 13 2404.0 14 2794.0 15 3659.0 16 4729.0 17 6445.0 18 5221.0 19 3350.0 20 3218.0 21 3215.0 22 3473.0 23 3787.0 24 4256.0 25 4598.0 26 5314.0 27 5900.0 28 7018.0 29 8372.0 30 10117.0 31 12465.0 32 15763.0 33 20273.0 34 27627.0 35 39056.0 36 55630.0 37 77093.0 38 62821.0 39 8812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04075929851609 19.00028907303912 15.399402582385816 21.559549046058972 2 14.949033281880988 20.66794936318693 40.73051091937554 23.65250643555654 3 18.712571606070895 26.15406472057078 30.33448673587082 24.79887693748751 4 12.135127455560319 16.950891201641294 38.00646307810114 32.907518264697245 5 13.018915632186676 37.73951573091881 33.70955895632909 15.532009680565423 6 30.142152037945714 36.94003720451171 17.553261892328315 15.364548865214264 7 27.040768447824902 34.330868570301085 20.891693997223232 17.736668984650773 8 26.503858453586666 33.48637209112063 19.883007163465123 20.126762291827585 9 26.864034032396223 16.72144637683949 20.66991655518234 35.744603035581946 10-11 24.611719121336648 27.10439391842702 26.843995740251614 21.43989121998472 12-13 26.182778556517448 24.87658145984611 27.777383394596416 21.163256589040024 14-15 22.833441613128326 25.657859910007456 27.427753314564836 24.08094516229938 16-17 20.90245393163257 29.351289048024682 27.582547680579815 22.16370933976293 18-19 20.925350221610515 26.842543443841826 31.507312425107198 20.724793909440464 20-21 21.95924161493672 25.70148212309321 31.514724989172805 20.82455127279727 22-23 21.496078148308552 25.679587122852602 31.732832876184975 21.091501852653867 24-25 21.37355545477838 26.025210599566712 31.605031534300394 20.996202411354513 26-27 20.919878985450588 26.447813979225437 31.632072464378297 21.00023457094568 28-29 20.55879122267312 26.66102814408791 31.757354241584622 21.022826391654345 30-31 20.742634716849455 26.34608558588368 31.611909589156472 21.299370108110384 32-33 20.66361704738781 26.61747330781022 31.33580062131381 21.383109023488164 34-35 21.38901857351193 26.552806241547106 31.017620530493 21.04055465444797 36-37 21.254332346462604 26.394802026353176 31.13731538279236 21.21355024439186 38-39 20.915416373942584 26.660226736856284 31.128920497743216 21.295436391457912 40-41 20.94461459253075 26.742868416050964 31.092070029904896 21.220446961513385 42-43 21.0177121567014 26.827366924234603 30.42321263370134 21.731708285362657 44-45 21.04297526825706 26.86059535682791 30.412692989284157 21.683736385630876 46-47 21.397788659359698 27.35878340171084 29.750472225898434 21.49295571303103 48-49 21.419409952834727 27.440321493887765 29.361223409375302 21.779045143902202 50-51 21.288464176306523 27.794556117329122 29.201610399736253 21.715369306628098 52-53 21.588573381533916 27.94919684417766 28.76330369735683 21.698926076931592 54-55 21.08121050529163 27.609718886712454 29.053787401157948 22.25528320683797 56-57 21.390672156605188 27.828358828348005 28.78031705477549 22.000651960271323 58-59 21.631280749660174 27.436094838267312 28.968375215022075 21.96424919705044 60-61 21.336268336250296 27.46080147231294 29.59637695541562 21.60655323602115 62-63 21.141504338443067 27.648266727936978 29.490105125894843 21.720123807725113 64-65 20.995355696398953 27.65134884481819 29.26260102653424 22.09069443224862 66-67 21.050694392656013 27.69668043503886 29.010770122712522 22.2418550495926 68-69 20.972504718064805 27.521984558185856 29.275577083375932 22.229933640373403 70-71 21.186712877028068 27.552386646472694 29.033010895363848 22.227889581135393 72-73 21.194481935653055 27.315573326789412 29.227865551313236 22.262079186244296 74-75 20.78852744772764 27.988586153011475 28.743935491554502 22.47895090770639 76-77 20.988967231317616 27.432388051262496 28.94456668322805 22.634078034191838 78-79 21.00903922450816 27.46689988523529 28.93610468851126 22.58795620174529 80-81 20.99521089044235 27.897976688428784 28.66199665327737 22.44481576785149 82-83 21.05660749578081 27.643353169233066 28.80487801944135 22.495161315544777 84-85 21.004182288603175 27.33222431116131 28.714412356575885 22.949181043659628 86-87 21.08407577272033 27.3739717409948 28.60337197987445 22.938580506410418 88-89 20.869689686944433 27.10987136646964 28.651934073812118 23.368504872773812 90-91 20.90656587831539 27.058400385895563 29.007966914988696 23.02706682080035 92-93 21.088401006558026 27.06556099250855 28.999167616930578 22.846870384002848 94-95 20.96788937857239 27.181443775139048 28.484825157329535 23.365841688959026 96-97 20.93218504173783 27.310856647982874 28.38196733142486 23.374990978854434 98-99 21.168253754396773 27.521737247561628 27.882380679713027 23.427628318328576 100-101 21.39045987822861 26.91864507080879 28.168834511675573 23.52206053928703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 804.0 1 509.0 2 227.0 3 259.5 4 256.0 5 240.5 6 358.5 7 747.0 8 1203.5 9 1383.5 10 1262.5 11 1098.5 12 1072.5 13 1102.5 14 1144.5 15 1193.5 16 1244.5 17 1294.0 18 1331.0 19 1405.0 20 1491.5 21 1588.0 22 1786.5 23 2080.0 24 2373.0 25 2775.0 26 3288.0 27 3861.5 28 4427.0 29 4973.0 30 5594.0 31 6227.0 32 6945.0 33 7562.0 34 8067.0 35 8495.5 36 9214.5 37 10060.5 38 10610.5 39 10727.5 40 11021.0 41 11844.0 42 12595.0 43 13271.5 44 13634.0 45 14003.5 46 14215.0 47 14911.0 48 16801.0 49 16704.5 50 14900.0 51 13944.5 52 13319.5 53 13253.5 54 13089.0 55 11934.5 56 10629.5 57 9490.0 58 8526.0 59 7512.0 60 6491.5 61 5552.0 62 4513.5 63 3499.0 64 2694.5 65 2083.0 66 1505.5 67 1207.5 68 881.0 69 558.5 70 449.0 71 357.0 72 280.0 73 231.5 74 184.0 75 146.0 76 100.5 77 68.5 78 41.5 79 23.0 80 16.5 81 15.0 82 11.5 83 9.0 84 5.0 85 2.5 86 2.0 87 3.0 88 4.0 89 3.0 90 1.5 91 1.0 92 1.5 93 0.5 94 0.5 95 2.0 96 4.5 97 4.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31673306294559345 2 0.28047325058411915 3 0.2751903640148977 4 0.27783180729950846 5 0.28191403783027047 6 0.2156378245073108 7 0.20363126412271665 8 0.2060325761996355 9 0.2005095584227222 10-11 0.22128090788807006 12-13 0.21167565958039475 14-15 0.20387139533040854 16-17 0.20675296982271113 18-19 0.20291087049964102 20-21 0.19666745909965205 22-23 0.19666745909965205 24-25 0.18742240760351456 26-27 0.18886319484966585 28-29 0.20303093610348696 30-31 0.21407697165731357 32-33 0.20843388827655432 34-35 0.21635821813038644 36-37 0.19582699987273047 38-39 0.19282535977658194 40-41 0.1892233916612037 42-43 0.19702765591118987 44-45 0.20159014885733564 46-47 0.20507205136886794 48-49 0.211075331561165 50-51 0.21371677484577573 52-53 0.22884504093036434 54-55 0.21059506914578124 56-57 0.18478096431890384 58-59 0.18826286683043614 60-61 0.1840605706958282 62-63 0.1907842445112009 64-65 0.18454083311121197 66-67 0.1805786681842959 68-69 0.1799783401650662 70-71 0.1929454253804279 72-73 0.1887431292458199 74-75 0.1929454253804279 76-77 0.19534673745734668 78-79 0.19354575339965757 80-81 0.19414608141888728 82-83 0.1869421451881308 84-85 0.18189938982660125 86-87 0.1797382089573743 88-89 0.18297998026121473 90-91 0.18790267001889832 92-93 0.1837003738842904 94-95 0.180338536976604 96-97 0.17985827456122025 98-99 0.1904240476996631 100-101 0.17817735610737706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 416439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.38501389313313 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40184516326734 54.532308047131735 2 10.074205719854847 13.174041619099807 3 2.829596424557744 5.550396045950038 4 1.1776834507452645 3.0801139515476836 5 0.6371301073125649 2.082938045918273 6 0.39874850754257435 1.5643306023322399 7 0.2688675756355229 1.2305937117839183 8 0.19265284984638195 1.00772874110059 9 0.1534955333056712 0.9032676819952672 >10 0.7927018536083007 9.359345049431584 >50 0.047736009368284565 2.0909770618376307 >100 0.022399204381002326 2.626146181009592 >500 0.0018360003590985514 0.8571349301704374 >1k 0.001101600215459131 1.940678330691217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3603 0.8651927413138539 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.6253016648296629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1876 0.45048614562997225 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 854 0.20507205136886794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 823 0.19762798393041955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.1690523702150855 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 607 0.14575964306897288 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 582 0.1397563628766758 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 474 0.11382219244595246 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.11238140519980117 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4013120769188285E-4 0.0 10-11 0.0 0.0 0.0 2.4013120769188285E-4 0.0 12-13 0.0 0.0 0.0 2.4013120769188285E-4 0.0 14-15 0.0 0.0 0.0 2.4013120769188285E-4 0.0 16-17 0.0 0.0 0.0 2.4013120769188285E-4 0.0 18-19 0.0 0.0 0.0 2.4013120769188285E-4 0.0 20-21 0.0 0.0 0.0 2.4013120769188285E-4 0.0 22-23 0.0 0.0 0.0 3.6019681153782426E-4 0.0 24-25 0.0 0.0 0.0 9.605248307675314E-4 0.0 26-27 0.0 0.0 0.0 0.0018009840576891213 0.0 28-29 0.0 0.0 0.0 0.002881574492302594 0.0 30-31 0.0 0.0 0.0 0.005763148984605188 0.0 32-33 0.0 0.0 0.0 0.008044395457678075 0.0 34-35 0.0 0.0 0.0 0.011766429176902259 0.0 36-37 0.0 0.0 0.0 0.01608879091535615 0.0 38-39 0.0 0.0 0.0 0.0202910870499641 0.0 40-41 0.0 0.0 0.0 0.027134826469182763 0.0 42-43 0.0 0.0 0.0 0.04070223970377414 0.0 44-45 0.0 0.0 0.0 0.0680771973806488 0.0 46-47 0.0 0.0 0.0 0.09040939969599389 0.0 48-49 0.0 0.0 0.0 0.14395865901128374 0.0 50-51 0.0 0.0 0.0 0.20615264180348142 0.0 52-53 0.0 0.0 0.0 0.27903246333796783 0.0 54-55 0.0 0.0 0.0 0.39477570544545537 0.0 56-57 0.0 0.0 0.0 0.5099186195337132 0.0 58-59 0.0 0.0 0.0 0.6668443637603587 0.0 60-61 0.0 0.0 0.0 0.8579888050830974 0.0 62-63 0.0 0.0 0.0 1.1032828337403557 0.0 64-65 0.0 0.0 0.0 1.3025917361246186 0.0 66-67 0.0 0.0 0.0 1.5794630185933594 0.0 68-69 0.0 0.0 0.0 1.9377387804696484 0.0 70-71 0.0 0.0 0.0 2.2391034461229617 0.0 72-73 0.0 0.0 0.0 2.6493676144645435 0.0 74-75 0.0 0.0 0.0 3.1205050439560176 0.0 76-77 0.0 0.0 0.0 3.5246458665014564 0.0 78-79 0.0 0.0 0.0 3.9359906252776518 0.0 80-81 0.0 0.0 0.0 4.453833574665197 0.0 82-83 1.2006560384594143E-4 0.0 0.0 5.164982146244708 0.0 84-85 2.4013120769188285E-4 0.0 0.0 5.885135638112665 0.0 86-87 2.4013120769188285E-4 0.0 0.0 6.652835109103615 0.0 88-89 2.4013120769188285E-4 0.0 0.0 7.673512807397962 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3720 0.0 36.951694 1 TAGAGTG 115 1.0236727E-7 33.08011 5 GGTATCA 2235 0.0 32.773487 1 CAACGCA 4580 0.0 29.694456 5 TATCAAC 4600 0.0 29.66515 2 ATCAACG 4625 0.0 29.504793 3 TCAACGC 4655 0.0 29.318178 4 AACGCAG 4685 0.0 29.098885 6 GTACATG 2375 0.0 28.238068 1 ACTCGAG 60 5.3783133E-6 27.709002 38-39 TTAGGAC 70 0.00834521 27.169672 3 TACATGG 2490 0.0 26.160357 2 CGCAGAG 5160 0.0 26.144041 8 ACGCAGA 5175 0.0 26.06826 7 CGCGGAA 55 9.4874944E-5 25.903479 82-83 ACATGGG 2430 0.0 24.84963 3 CTAGGTA 115 1.2702344E-4 24.810081 4 GCAGAGT 5305 0.0 24.802671 9 GAGTACT 2830 0.0 24.670769 12-13 AGTACTT 2845 0.0 24.540693 12-13 >>END_MODULE