FastQCFastQC Report
Thu 26 May 2016
SRR937514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416439
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45431.0909160765442238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.8498243440215735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27180.6526766225065376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10880.2612627539687685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9910.2379700268226559No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.22164110469960785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.15296357929972937No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5370.12895045853054107No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.1022958944767421No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG4260.1022958944767421No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG405.3822185E-447.503545
TACACCG459.6144615E-442.2253725
CTGGACG901.19271135E-842.2253724
GTATCAA42400.030.0366441
GGTATCA29000.029.9871791
AGGTAAG1601.897206E-929.6897132
ATCGTCA350.00839212827.14162368-69
TTAACCG700.00838739327.1416239
GGTAAGG1806.666596E-926.3908583
ATATACC908.934035E-426.3908584
CTTCGCG559.468752E-525.91102424-25
GTGTAAG1309.967365E-625.5880471
AGGTCGT950.001225566124.9988616
GTACATG25650.024.825611
CACGAGC1351.3405273E-524.6285089
AAGGTAA1951.5565092E-824.3695721
GTAAGGT1951.5625119E-824.360794
TATCAAC53250.023.8186782
ACGCTGG1000.00165231923.7489198
ATCAACG53800.023.5751783
Produced by FastQC (version 0.11.5)