##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 416439 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.066912561023344 31.0 31.0 34.0 30.0 34.0 2 32.282002886377114 33.0 31.0 34.0 31.0 34.0 3 32.329546464188034 34.0 31.0 34.0 30.0 34.0 4 36.006296240265684 37.0 35.0 37.0 35.0 37.0 5 35.852043156380645 37.0 35.0 37.0 35.0 37.0 6 35.95323204598993 37.0 35.0 37.0 35.0 37.0 7 35.909199186435465 37.0 35.0 37.0 35.0 37.0 8 35.89823959811641 37.0 35.0 37.0 35.0 37.0 9 37.55985390417324 39.0 37.0 39.0 35.0 39.0 10-11 37.472942015517276 39.0 37.0 39.0 35.0 39.0 12-13 37.37819824752245 39.0 37.0 39.0 34.0 39.0 14-15 38.495684842197775 40.0 38.0 41.0 34.0 41.0 16-17 38.48158313702607 40.0 38.0 41.0 34.0 41.0 18-19 38.550423951647176 40.0 38.0 41.0 34.0 41.0 20-21 38.49945610281458 40.0 38.0 41.0 34.0 41.0 22-23 38.3677477373637 40.0 38.0 41.0 34.0 41.0 24-25 38.20182907940899 40.0 37.5 41.0 33.5 41.0 26-27 37.9521538568674 40.0 37.0 41.0 33.0 41.0 28-29 37.874045178285414 40.0 37.0 41.0 33.0 41.0 30-31 37.896927281066375 40.0 37.0 41.0 33.0 41.0 32-33 37.749551554969635 40.0 36.5 41.0 33.0 41.0 34-35 37.57581302423644 40.0 36.0 41.0 33.0 41.0 36-37 37.36134463871059 40.0 36.0 41.0 33.0 41.0 38-39 37.16712651792939 40.0 35.5 41.0 32.0 41.0 40-41 36.94623222128571 39.5 35.0 41.0 31.5 41.0 42-43 36.64894138157089 39.0 35.0 41.0 31.0 41.0 44-45 36.207274054543404 39.0 35.0 41.0 30.0 41.0 46-47 35.65192981445062 39.0 35.0 41.0 28.0 41.0 48-49 35.15460247479223 39.0 35.0 40.5 26.0 41.0 50-51 34.85309853303845 39.0 35.0 40.0 24.0 41.0 52-53 34.58101666750713 38.0 34.5 40.0 23.5 41.0 54-55 34.33867985467259 38.0 34.0 40.0 23.0 41.0 56-57 34.04996410038445 38.0 34.0 40.0 22.0 41.0 58-59 33.77152356047344 38.0 34.0 40.0 21.0 41.0 60-61 33.413008868045495 37.0 33.0 40.0 19.5 41.0 62-63 33.03082204116329 36.5 33.0 40.0 18.0 41.0 64-65 32.66973914546908 36.0 32.5 39.0 14.0 41.0 66-67 32.2533060064019 35.5 32.0 39.0 9.5 40.5 68-69 31.787558321867067 35.0 31.5 38.5 7.5 40.0 70-71 31.37489284144857 35.0 31.0 38.0 4.0 40.0 72-73 30.93789366509861 35.0 31.0 37.0 2.0 39.5 74-75 30.50876598973679 35.0 30.5 37.0 2.0 39.0 76-77 29.199206846620992 33.5 28.5 35.0 2.0 37.5 78-79 29.806474657752993 34.0 30.0 36.0 2.0 38.0 80-81 29.631246112876077 34.0 30.0 35.5 2.0 37.0 82-83 29.29692463962309 34.0 30.0 35.0 2.0 37.0 84-85 28.968057746752827 34.0 29.0 35.0 2.0 36.5 86-87 28.635043787925724 34.0 29.0 35.0 2.0 36.0 88-89 28.27498625248836 34.0 29.0 35.0 2.0 35.5 90-91 27.81535470981344 34.0 27.5 35.0 2.0 35.0 92-93 27.390913194969734 33.5 27.0 35.0 2.0 35.0 94-95 26.953080763329083 33.0 26.0 35.0 2.0 35.0 96-97 26.51463719776486 33.0 25.0 35.0 2.0 35.0 98-99 25.86568621094566 33.0 24.0 35.0 2.0 35.0 100-101 24.395419257082068 31.5 17.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11758108321479455 1101 2 0.12904597252800443 1101 3 0.0056582170179737545 1101 4 0.06268015356906176 1101 5 -0.018633828192015756 1101 6 -0.015433400891907922 1101 7 0.05460668997942264 1101 8 0.1160676814688486 1101 9 0.0414744002041445 1101 10-11 0.05669275596741841 1101 12-13 0.11548209569264856 1101 14-15 0.23123944232182225 1101 16-17 0.09325003987070346 1101 18-19 0.023751648941626513 1101 20-21 0.007120627051321549 1101 22-23 -0.2442415535935396 1101 24-25 0.03075148253844162 1101 26-27 -0.04977501479925195 1101 28-29 -0.060393759102645106 1101 30-31 0.25734740330818084 1101 32-33 0.34862440273810336 1101 34-35 0.298211717878651 1101 36-37 0.43176079901833475 1101 38-39 0.2900879794353415 1101 40-41 0.47159803525503463 1101 42-43 0.5361914592754289 1101 44-45 0.7793984010968487 1101 46-47 0.9069312082924057 1101 48-49 0.9302438387855503 1101 50-51 1.0117347281207358 1101 52-53 1.1476493477157064 1101 54-55 0.9176288863331479 1101 56-57 0.9412569011150538 1101 58-59 1.1757655834369416 1101 60-61 1.2303188394048234 1101 62-63 0.9971457261920449 1101 64-65 1.226600208021523 1101 66-67 1.2124806152711933 1101 68-69 1.3760545961829713 1101 70-71 1.3056999321256448 1101 72-73 1.4413751042598975 1101 74-75 1.3842514734579012 1101 76-77 1.2346375783752919 1101 78-79 1.4929834937450401 1101 80-81 1.3719500288773219 1101 82-83 1.4033110943809959 1101 84-85 1.435102456546609 1101 86-87 0.8986578505591467 1101 88-89 1.1360912982633735 1101 90-91 1.4278570574292644 1101 92-93 1.6638065644378202 1101 94-95 1.293141507749624 1101 96-97 1.3407076649180851 1101 98-99 1.419922503538519 1101 100-101 1.5262713979174478 1102 1 0.033399281710487116 1102 2 0.04507055517338898 1102 3 0.021698851763893856 1102 4 -0.007169415317378025 1102 5 -0.24957769170329414 1102 6 -0.1094528468651248 1102 7 -0.08043733863917879 1102 8 -0.09700102152142875 1102 9 0.19116023766515866 1102 10-11 0.04140886310371883 1102 12-13 -0.10936770250951611 1102 14-15 0.05085396259333663 1102 16-17 0.08516092778117468 1102 18-19 0.026996466406309594 1102 20-21 -0.05267918032347296 1102 22-23 -0.035122813912742856 1102 24-25 -0.08501282676260047 1102 26-27 0.07587280211507164 1102 28-29 -0.04676667232957499 1102 30-31 -0.07199010357128799 1102 32-33 0.23124341759776001 1102 34-35 0.4745096448645256 1102 36-37 0.3736464621994031 1102 38-39 0.1404136077604221 1102 40-41 0.27113023118239 1102 42-43 0.39215825435890395 1102 44-45 0.375854326615098 1102 46-47 0.6897233565220873 1102 48-49 0.9047324647257469 1102 50-51 0.5863139337710805 1102 52-53 0.5435228113003703 1102 54-55 0.7869303906442866 1102 56-57 0.962795380293187 1102 58-59 1.0857935599550146 1102 60-61 1.1217378450918503 1102 62-63 1.207812346995567 1102 64-65 1.2442460528206425 1102 66-67 1.3065975204262124 1102 68-69 1.3517872599143992 1102 70-71 1.2006045367853382 1102 72-73 1.1907731504899495 1102 74-75 1.1098121503979144 1102 76-77 0.9806876150658042 1102 78-79 0.966956159310989 1102 80-81 1.2506936080371105 1102 82-83 1.471566919000331 1102 84-85 1.4278618126280591 1102 86-87 1.3741841301408755 1102 88-89 1.5379785666544947 1102 90-91 1.2864437846352814 1102 92-93 1.2470940346267732 1102 94-95 0.9749774094190435 1102 96-97 1.4421397416012596 1102 98-99 1.5618517367067462 1102 100-101 1.6274168299482668 1103 1 0.06231899463291768 1103 2 -0.13023209871674624 1103 3 -0.03586453795967515 1103 4 0.06008974316679172 1103 5 -0.10247508513363357 1103 6 0.06696873915956303 1103 7 -0.10254487442529125 1103 8 -0.06100295377427756 1103 9 -0.10437586391613252 1103 10-11 -0.06663956151869854 1103 12-13 -0.045965655898847047 1103 14-15 -0.07352742074196783 1103 16-17 -0.11737318239666195 1103 18-19 -0.143118187168362 1103 20-21 -0.043091854324813994 1103 22-23 -0.21664860357321913 1103 24-25 0.02730183469881098 1103 26-27 -0.0834715135125137 1103 28-29 -0.12430324711564822 1103 30-31 -0.05651975773746187 1103 32-33 0.20115301576803546 1103 34-35 0.23722702331004797 1103 36-37 0.02564624531387949 1103 38-39 0.21720094164582804 1103 40-41 0.16294132389109706 1103 42-43 0.1586095142666295 1103 44-45 0.21687723761316846 1103 46-47 0.16657329145643018 1103 48-49 0.32556924851059676 1103 50-51 0.37377844341262545 1103 52-53 0.39511396237099206 1103 54-55 0.3724744775562314 1103 56-57 0.48423993646654395 1103 58-59 0.3875527973066397 1103 60-61 0.7827704173541647 1103 62-63 0.6289646006333314 1103 64-65 0.5192149987570005 1103 66-67 0.5591681068057959 1103 68-69 0.7036864870132611 1103 70-71 0.4655942840151184 1103 72-73 0.5773420215022114 1103 74-75 0.5603104941811843 1103 76-77 0.5322003915948486 1103 78-79 0.8695326607503233 1103 80-81 0.78872251296238 1103 82-83 0.5666169998394821 1103 84-85 0.6892039396835443 1103 86-87 0.6731016742080698 1103 88-89 0.6772585067152264 1103 90-91 0.8071193830421564 1103 92-93 0.8438570181829128 1103 94-95 0.7942674050813316 1103 96-97 1.0566725504659615 1103 98-99 1.01173392843355 1103 100-101 1.4182233483440996 1104 1 -0.058510396010916566 1104 2 0.01253106770579393 1104 3 -0.08526697745829637 1104 4 -0.05014623490105663 1104 5 -0.1301511451230084 1104 6 -0.11902844802852997 1104 7 -0.07446374359712848 1104 8 -0.05373598292641191 1104 9 0.005907102369015149 1104 10-11 -0.1339153467080152 1104 12-13 0.022330097662539572 1104 14-15 0.04614589772796762 1104 16-17 -0.025024322428038204 1104 18-19 -0.03877598980488273 1104 20-21 -0.18965782578190016 1104 22-23 -0.09845784437324312 1104 24-25 -0.1994057819291868 1104 26-27 -0.1253047993175329 1104 28-29 -0.00843354382193695 1104 30-31 -0.03336509845064484 1104 32-33 -0.056241147323206064 1104 34-35 -0.08386128861393871 1104 36-37 -0.035622339336207176 1104 38-39 0.04362322637497584 1104 40-41 0.20247704789850474 1104 42-43 0.18697338454025925 1104 44-45 0.05549377487320584 1104 46-47 0.010476003162658287 1104 48-49 0.13561856993592158 1104 50-51 0.252128379249406 1104 52-53 0.35156236606783864 1104 54-55 0.40049160719186006 1104 56-57 0.4136401399741274 1104 58-59 0.2515648457336681 1104 60-61 0.22357498525700237 1104 62-63 0.37494791819590034 1104 64-65 0.31172880357118515 1104 66-67 0.4504722080670298 1104 68-69 0.31503640988777093 1104 70-71 0.6760415470324723 1104 72-73 0.6091303606469651 1104 74-75 0.5906997186487573 1104 76-77 0.39184739666309554 1104 78-79 0.5708569183364887 1104 80-81 0.6708825451088032 1104 82-83 0.4664543233906251 1104 84-85 0.5830721393543001 1104 86-87 0.8452729835266446 1104 88-89 0.5826517818911867 1104 90-91 0.6949949647238824 1104 92-93 0.7833240421760657 1104 94-95 0.8292088156352087 1104 96-97 0.7655748733041818 1104 98-99 0.8543728191227551 1104 100-101 0.8017549503506629 1105 1 -0.14559166458215955 1105 2 -0.09369818303240862 1105 3 -0.25201147200686336 1105 4 -0.0059237354412076115 1105 5 -0.033354169026623026 1105 6 -0.09235787835167031 1105 7 0.11185852888409187 1105 8 0.015205581111992217 1105 9 -0.18728260948196862 1105 10-11 -0.12373383350166733 1105 12-13 -0.16133905717313013 1105 14-15 -0.22502967500393822 1105 16-17 -0.2474912571943335 1105 18-19 -0.18605189777747455 1105 20-21 -0.20748273395661698 1105 22-23 -0.4194177060951745 1105 24-25 -0.319197993100623 1105 26-27 -0.229639463856131 1105 28-29 -0.23985765466742492 1105 30-31 -0.38241617909659453 1105 32-33 -0.16649421571649015 1105 34-35 -0.11816363715740152 1105 36-37 -0.09386779685961244 1105 38-39 -0.11007406265919428 1105 40-41 0.01533119380139425 1105 42-43 -0.5511474089788848 1105 44-45 -0.00442788613641909 1105 46-47 -0.012612160726604316 1105 48-49 -0.1823617616805464 1105 50-51 -0.40237158241745163 1105 52-53 -0.23646096835741304 1105 54-55 -0.06969450599078897 1105 56-57 0.04759702565078072 1105 58-59 0.2001310702915049 1105 60-61 0.062122786677413444 1105 62-63 0.23929624327018928 1105 64-65 -0.15134477049424078 1105 66-67 -0.24903666314989792 1105 68-69 0.002909431956037878 1105 70-71 -0.055353523558576256 1105 72-73 -0.12915189668686367 1105 74-75 0.3887781830930308 1105 76-77 0.3170582869908287 1105 78-79 0.5249094277629567 1105 80-81 0.2658511467857032 1105 82-83 -0.03903196147800969 1105 84-85 0.0702642255364836 1105 86-87 0.19467370883603863 1105 88-89 0.27443246059678117 1105 90-91 -0.007769899820750936 1105 92-93 0.4652867916608905 1105 94-95 0.5519127188777269 1105 96-97 0.41903635547230067 1105 98-99 0.5590078760532684 1105 100-101 0.22121122385874514 1106 1 -0.27573529051323575 1106 2 -0.12602649124011123 1106 3 -0.21819700962402777 1106 4 -0.08292576687497188 1106 5 -0.18413644922192418 1106 6 -0.04400262691719092 1106 7 -0.14920922121391555 1106 8 -0.18610237711887834 1106 9 -0.332612093782906 1106 10-11 -0.12632836645010315 1106 12-13 -0.2631584377640337 1106 14-15 -0.3768953865403404 1106 16-17 -0.17382327187767999 1106 18-19 -0.2513678391866492 1106 20-21 -0.4118182412804714 1106 22-23 -0.15051468032804394 1106 24-25 -0.2750321283157078 1106 26-27 -0.05748970798457265 1106 28-29 -0.01007576650319919 1106 30-31 0.001968510307875704 1106 32-33 -0.0815000961826513 1106 34-35 -0.021800616379749727 1106 36-37 -0.16841072426258563 1106 38-39 -0.1557260816192354 1106 40-41 -0.11972173039405476 1106 42-43 -0.2657666042459965 1106 44-45 -0.05884358163519465 1106 46-47 0.04418066866859505 1106 48-49 -0.2465176474925883 1106 50-51 -0.0177730578798716 1106 52-53 0.0023768376941220026 1106 54-55 0.08639623138402897 1106 56-57 -0.3238506382003905 1106 58-59 -0.3227350332759755 1106 60-61 -0.17755966814991098 1106 62-63 -0.3919789269069298 1106 64-65 -0.16524761806542898 1106 66-67 0.12065398869086508 1106 68-69 0.1060403346966936 1106 70-71 -0.08155723716205543 1106 72-73 0.13597693898083563 1106 74-75 -0.24931119999260787 1106 76-77 0.016265315038872075 1106 78-79 -0.13594805112082753 1106 80-81 -0.2167159439873103 1106 82-83 -0.07018952233192266 1106 84-85 0.06028168873902473 1106 86-87 -0.18530954074519812 1106 88-89 -0.04560389137584764 1106 90-91 -0.10701613097653251 1106 92-93 -0.4425841231970864 1106 94-95 -0.4455765002676415 1106 96-97 -0.29164470942665943 1106 98-99 -0.1316867317374566 1106 100-101 0.025120842875818283 1107 1 -0.12091378033195 1107 2 -0.11862792322101967 1107 3 -0.047100680979326626 1107 4 -0.1502491812861777 1107 5 -0.12895948278715252 1107 6 -0.28523555232215614 1107 7 -0.27569579004547506 1107 8 -0.17353445958472946 1107 9 -0.2626075236310754 1107 10-11 -0.2269427979739902 1107 12-13 -0.25599091630969895 1107 14-15 -0.3073325977321346 1107 16-17 -0.4443686433294616 1107 18-19 -0.18584201782881138 1107 20-21 -0.44423331262562016 1107 22-23 -0.4553971258670657 1107 24-25 -0.06636661265023491 1107 26-27 -0.0560907337297607 1107 28-29 -0.08109846492395434 1107 30-31 -0.08715706730853867 1107 32-33 -0.3182314220344793 1107 34-35 0.19609660598352008 1107 36-37 0.032102148331745184 1107 38-39 -0.22188410731364883 1107 40-41 0.2236820171304501 1107 42-43 0.41655706406253046 1107 44-45 0.5077511474563607 1107 46-47 0.16032092155033695 1107 48-49 -0.12454283410712463 1107 50-51 0.17855812578945063 1107 52-53 -0.012189251779297194 1107 54-55 0.14414517771013635 1107 56-57 0.2603493313319305 1107 58-59 0.4912445533791754 1107 60-61 0.38958347916131686 1107 62-63 0.22878225727018275 1107 64-65 0.5342750549655975 1107 66-67 0.7472954348900238 1107 68-69 0.42827412335255843 1107 70-71 0.3719160782292015 1107 72-73 0.4832957832402158 1107 74-75 0.44657312438661023 1107 76-77 0.530618130760196 1107 78-79 0.8657784506818444 1107 80-81 0.975761078304874 1107 82-83 0.4649117827003231 1107 84-85 0.7588903314039293 1107 86-87 0.9327095523335593 1107 88-89 0.9637801537163142 1107 90-91 0.7861819597155915 1107 92-93 0.9177799655654191 1107 94-95 0.27520499569539325 1107 96-97 0.13063731972075487 1107 98-99 0.5065298934593123 1107 100-101 0.22431344938574682 1108 1 -0.21828848200259543 1108 2 -0.3532992185128414 1108 3 -0.5061218507403566 1108 4 -0.41196693846933385 1108 5 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-0.6723158985929274 2316 52-53 -0.7822982629384185 2316 54-55 -0.6063869773566282 2316 56-57 -0.501567716981306 2316 58-59 -0.1904751137820142 2316 60-61 -0.35303407873070825 2316 62-63 0.005922165675002589 2316 64-65 -0.016598969416783405 2316 66-67 -0.1852402033735423 2316 68-69 -0.0922057837621928 2316 70-71 0.4316400026309317 2316 72-73 0.4127739487565698 2316 74-75 0.8348463618245283 2316 76-77 0.9543619222844164 2316 78-79 0.4156333174818201 2316 80-81 0.11287002496035825 2316 82-83 -0.004145819054176769 2316 84-85 -0.05748714392342791 2316 86-87 0.2484742430385829 2316 88-89 0.46601197981448905 2316 90-91 0.43757846361805974 2316 92-93 0.037318119471112965 2316 94-95 0.17181855436145455 2316 96-97 0.04728571035482432 2316 98-99 0.33674340052246166 2316 100-101 -0.196385770120159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 3.0 5 5.0 6 30.0 7 69.0 8 149.0 9 342.0 10 533.0 11 911.0 12 1386.0 13 1945.0 14 2436.0 15 3509.0 16 4915.0 17 7113.0 18 6180.0 19 3446.0 20 2924.0 21 2877.0 22 2830.0 23 3067.0 24 3366.0 25 3972.0 26 4481.0 27 5522.0 28 6643.0 29 7919.0 30 9812.0 31 12015.0 32 14920.0 33 19850.0 34 26717.0 35 38458.0 36 57468.0 37 84377.0 38 69234.0 39 7011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.797298660862296 19.819670476986722 16.070132952781822 20.31289790936916 2 15.67168300759535 21.876673414353604 39.35822533432267 23.093418243728372 3 19.21049661535063 26.221127223915147 30.955313983560618 23.613062177173607 4 12.807638093454262 18.029291204714255 37.02967301333449 32.133397688497 5 13.076368876080691 38.44644572526417 33.22814601344861 15.249039385206531 6 30.190736218269663 37.63192208222573 16.746030030808832 15.431311668695773 7 26.89613604873703 35.249580370714554 20.133320846510532 17.72096273403788 8 27.05054041528291 34.13393077977807 19.17615785265069 19.639370952288328 9 27.1691652318827 16.772684594862632 20.348958671017844 35.70919150223682 10-11 25.18856303084005 27.543529784674348 26.020257468680885 21.247649715804716 12-13 26.736268288436488 25.389663018485052 27.401225933107998 20.47284275997046 14-15 23.1266764159937 25.904274095365707 27.475932849709082 23.493116638931514 16-17 20.903901891993783 29.56135232290924 27.691810805424083 21.842934979672894 18-19 20.852033551132337 26.580003313810668 32.22164590732376 20.34631722773323 20-21 21.908850996184313 25.370102223855113 32.59120783596157 20.129838943999 22-23 21.3477574396811 25.56740888379269 32.725831917537654 20.35900175898855 24-25 21.525173014950603 25.82953524894462 32.622983493341145 20.02230824276363 26-27 20.961891508646723 26.161210961279156 32.716462210290096 20.16043531978402 28-29 20.593885602998846 26.530820527967684 32.68640121798058 20.188892651052885 30-31 20.82516881345513 25.954288293999557 32.64919459412731 20.571348298418005 32-33 20.696308462944152 26.194232528653654 32.518808276842464 20.590650731559723 34-35 21.288472021112337 26.128676708953773 32.082369806862474 20.50048146307142 36-37 21.409523855263142 25.8454729810044 32.32073883658691 20.424264327145544 38-39 21.088638751313223 26.33900645354945 32.13807594176797 20.434278853369353 40-41 20.917469305228376 26.367367129399504 32.11262633903165 20.602537226340473 42-43 21.011236764561083 26.5025780437607 31.443728521305186 21.04245667037303 44-45 21.21679285561631 26.747494831175757 31.074178931368102 20.961533381839835 46-47 21.67388260945781 27.135066600390452 30.315724511873288 20.875326278278454 48-49 21.900326338311256 27.292952869447866 29.77194739205502 21.03477340018586 50-51 21.61255503513724 27.554608903835742 29.670887778147314 21.161948282879706 52-53 21.90212732236894 28.064014177346504 28.873856675287378 21.16000182499718 54-55 21.47325298543124 27.86914770230454 29.139441790994596 21.51815752126962 56-57 21.811837988276793 27.902165743362172 28.976152569764118 21.309843698596914 58-59 21.915334538791996 27.375798136101565 29.189389082194513 21.519478242911926 60-61 21.61312941391176 27.45288025377066 29.9057004747394 21.02828985757818 62-63 21.262417785077766 27.673800964847196 29.85947521725871 21.20430603281633 64-65 21.33865944351994 27.49106111579367 29.59461049517456 21.57566894551183 66-67 21.312485141881524 27.63237833152034 29.29492674797509 21.76020977862304 68-69 21.366394598008352 27.51087194042825 29.432762061190232 21.689971400373164 70-71 21.544331822908035 27.71306241730482 29.03942714299093 21.70317861679622 72-73 21.520078570931155 27.585792877228116 29.01277257893713 21.88135597290359 74-75 21.344542658108388 28.239430024565422 28.511618748484175 21.904408568842015 76-77 21.35462816883145 27.63682075886264 28.939772691798797 22.06877838050711 78-79 21.35102620071607 27.626254985724202 28.80938144602211 22.213337367537623 80-81 21.30456081202769 28.064614505365732 28.64741294643393 21.983411736172645 82-83 21.29075326758541 27.836970120473826 28.601788016972474 22.270488594968292 84-85 20.95843684001359 27.903880164778233 28.582371706746613 22.55531128846156 86-87 21.017844150043583 27.699495004070222 28.377865665799796 22.9047951800864 88-89 20.827540167947767 27.36103006682852 28.353852545030605 23.457577220193112 90-91 20.98427305190205 27.459722058791748 28.6463874126781 22.909617476628107 92-93 20.994973981408773 27.24760045433479 28.576145502397743 23.181280061858693 94-95 20.834984235386216 27.50690977550133 28.322275291219125 23.33583069789333 96-97 20.926474225516824 27.690009821366395 28.028474758608102 23.35504119450868 98-99 21.02870672649186 27.875385263744324 27.41432946998995 23.681578539773867 100-101 20.757170594528652 27.420627249159775 27.540942662861077 24.281259493450495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 576.0 1 388.5 2 238.0 3 295.0 4 247.0 5 223.5 6 412.5 7 870.0 8 1378.5 9 1605.5 10 1455.0 11 1213.5 12 1214.0 13 1317.0 14 1373.5 15 1416.5 16 1423.5 17 1458.0 18 1522.5 19 1547.5 20 1637.5 21 1780.5 22 1923.0 23 2104.0 24 2360.5 25 2813.5 26 3365.0 27 3925.5 28 4394.0 29 4983.0 30 5639.5 31 6134.0 32 6792.5 33 7374.5 34 7861.0 35 8319.0 36 9120.5 37 9977.0 38 10534.0 39 10754.5 40 10976.0 41 11755.0 42 12522.0 43 13197.5 44 13667.0 45 14012.5 46 14132.5 47 14832.5 48 16716.5 49 16639.0 50 14926.0 51 13895.5 52 13417.5 53 13082.0 54 12633.0 55 11833.5 56 10445.0 57 9322.0 58 8586.5 59 7687.5 60 6541.5 61 5454.0 62 4577.5 63 3539.5 64 2535.0 65 1936.0 66 1459.5 67 1226.0 68 911.5 69 515.0 70 384.5 71 324.5 72 268.0 73 215.5 74 180.5 75 152.5 76 97.5 77 59.0 78 36.5 79 25.5 80 17.5 81 10.5 82 6.5 83 3.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04826637274606845 2 0.0 3 0.0 4 0.0 5 0.00936511709998343 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005162820965375481 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015608528499972386 24-25 2.4013120769188283E-4 26-27 0.002281246473072887 28-29 0.002881574492302594 30-31 7.203936230756485E-4 32-33 0.0 34-35 0.0 36-37 1.2006560384594141E-4 38-39 3.6019681153782426E-4 40-41 0.0 42-43 0.009124985892291548 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.2006560384594141E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.2006560384594141E-4 86-87 0.0 88-89 0.0 90-91 0.004802624153837657 92-93 0.001440787246151297 94-95 0.0 96-97 0.0 98-99 8.404592269215899E-4 100-101 0.008044395457678075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 416439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.561657641124526 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1912098864548 51.420183340261936 2 10.588361858421575 13.248509391338231 3 3.1041452445313964 5.826014161700939 4 1.3293394058220913 3.326627071835903 5 0.7376140201048629 2.307317789854699 6 0.4304322903895737 1.615713455342256 7 0.2913963670621372 1.2761167827806217 8 0.19971198602367926 0.9995450317153973 9 0.16721196306751143 0.9414951828237006 >10 0.8730327359052356 9.853838972402821 >50 0.05682696425060831 2.4523598201736196 >100 0.027645550169864433 3.1348119941125074 >500 0.0015358638983258017 0.7417853975340554 >1k 0.0015358638983258017 2.8556816081233323 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4543 1.0909160765442238 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3539 0.8498243440215735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2718 0.6526766225065376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1088 0.2612627539687685 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 991 0.2379700268226559 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.22164110469960785 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.15296357929972937 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 537 0.12895045853054107 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.1022958944767421 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 426 0.1022958944767421 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.4013120769188285E-4 0.0 14-15 0.0 0.0 0.0 2.4013120769188285E-4 0.0 16-17 0.0 0.0 0.0 2.4013120769188285E-4 0.0 18-19 0.0 0.0 0.0 2.4013120769188285E-4 0.0 20-21 0.0 0.0 0.0 2.4013120769188285E-4 0.0 22-23 0.0 0.0 0.0 8.4045922692159E-4 0.0 24-25 0.0 0.0 0.0 0.001440787246151297 0.0 26-27 0.0 0.0 0.0 0.002281246473072887 0.0 28-29 2.4013120769188285E-4 0.0 0.0 0.00336183690768636 0.0 30-31 2.4013120769188285E-4 0.0 0.0 0.006243411399988954 0.0 32-33 2.4013120769188285E-4 0.0 0.0 0.008524657873061841 0.0 34-35 2.4013120769188285E-4 0.0 0.0 0.01200656038459414 0.0 36-37 2.4013120769188285E-4 0.0 0.0 0.01608879091535615 0.0 38-39 2.4013120769188285E-4 0.0 0.0 0.020531218257655984 0.0 40-41 2.4013120769188285E-4 0.0 0.0 0.027735154488412468 0.0 42-43 2.4013120769188285E-4 0.0 0.0 0.04154269893069573 0.0 44-45 2.4013120769188285E-4 0.0 0.0 0.0686775253998785 0.0 46-47 2.4013120769188285E-4 0.0 0.0 0.09112979331906954 0.0 48-49 2.4013120769188285E-4 0.0 0.0 0.14515931504974317 0.0 50-51 2.4013120769188285E-4 0.0 0.0 0.20687303542655705 0.0 52-53 2.4013120769188285E-4 0.0 0.0 0.2792725945456598 0.0 54-55 2.4013120769188285E-4 0.0 0.0 0.3959763614839148 0.0 56-57 2.4013120769188285E-4 0.0 0.0 0.5115995379875564 0.0 58-59 2.4013120769188285E-4 0.0 0.0 0.6694858070449694 0.0 60-61 2.4013120769188285E-4 0.0 0.0 0.8624312324253972 0.0 62-63 2.4013120769188285E-4 0.0 0.0 1.1112071635941878 0.0 64-65 2.4013120769188285E-4 0.0 0.0 1.3132775748669072 0.0 66-67 2.4013120769188285E-4 0.0 0.0 1.5912294477702615 0.0 68-69 2.4013120769188285E-4 0.0 0.0 1.9511861281003942 0.0 70-71 2.4013120769188285E-4 0.0 0.0 2.252670859357553 0.0 72-73 2.4013120769188285E-4 0.0 0.0 2.664135683737594 0.0 74-75 2.4013120769188285E-4 0.0 0.0 3.1389951469482926 0.0 76-77 2.4013120769188285E-4 0.0 0.0 3.546617872005264 0.0 78-79 2.4013120769188285E-4 0.0 0.0 3.9637257797660643 0.0 80-81 2.4013120769188285E-4 0.0 0.0 4.487451943742061 0.0 82-83 2.4013120769188285E-4 0.0 0.0 5.208325829233093 0.0 84-85 2.4013120769188285E-4 0.0 0.0 5.937003978974111 0.0 86-87 2.4013120769188285E-4 0.0 0.0 6.717910666388114 0.0 88-89 2.4013120769188285E-4 0.0 0.0 7.754557089993973 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCG 40 5.3822185E-4 47.50354 5 TACACCG 45 9.6144615E-4 42.225372 5 CTGGACG 90 1.19271135E-8 42.225372 4 GTATCAA 4240 0.0 30.036644 1 GGTATCA 2900 0.0 29.987179 1 AGGTAAG 160 1.897206E-9 29.689713 2 ATCGTCA 35 0.008392128 27.141623 68-69 TTAACCG 70 0.008387393 27.141623 9 GGTAAGG 180 6.666596E-9 26.390858 3 ATATACC 90 8.934035E-4 26.390858 4 CTTCGCG 55 9.468752E-5 25.911024 24-25 GTGTAAG 130 9.967365E-6 25.588047 1 AGGTCGT 95 0.0012255661 24.998861 6 GTACATG 2565 0.0 24.82561 1 CACGAGC 135 1.3405273E-5 24.628508 9 AAGGTAA 195 1.5565092E-8 24.369572 1 GTAAGGT 195 1.5625119E-8 24.36079 4 TATCAAC 5325 0.0 23.818678 2 ACGCTGG 100 0.001652319 23.748919 8 ATCAACG 5380 0.0 23.575178 3 >>END_MODULE